LOCUS       sequence-246933-       16490 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16490)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX075
FEATURES             Location/Qualifiers
     source          1..16490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6604..6628)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6657..6746
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6780..7032
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7461..7713)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8181..9206)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8181..9206)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9216..9560)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9702..9719)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9702..9718)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9711..9733)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9858..9877)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10068..10089
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10336..10360
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11956..13104)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13403..14197)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14389..15156
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15849..16480
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggcttcccat ggcacttgaa gctttgaatt ctccaactac aacaactcca
     5881 ccaactttcc aatttgagaa caacggccgg cttcgatacc ttgaaaattg ggctaaaggt
     5941 aaaagatcaa aaaggcctcg tagtatggaa cgacagccta ctgaagaaga gtatttggct
     6001 ctttgtttga ttatgctagc gcgtagcgat ggttctgcta atcaagaaca gtctctacca
     6061 ctaccgccag caccaatgat gaaaataaac gcgcatccgg aggagaagat ggtgtacaag
     6121 tgttcagttt gtggtaaggg ttttggatct tatcaagctt taggaggaca caaagctagt
     6181 caccggaagc tcgtcgccgg cggcggcgga ggagatgacc agtccactac ctccacaacc
     6241 actaacgcga caggaactac tagctctgtt aacggtaacg gtaacggaag gggaaagact
     6301 cacgaatgtt caatctgcca caaatgtttt cctactggac aggctttggg tggacacaaa
     6361 aggtgtcact acgacggcgg taacagtaac ggtagcgtta gcgttagtgc tagcgtagga
     6421 ttgacgtcat cagaaggtgt agggtccact gtgagtcacc gtgattttga cttgaacatc
     6481 ccggcgttgc aggaattctg gccgggattt ggttccggcg aggatgaggt ggagagtcct
     6541 catccggcga agaaatcgcg gctatctctg cctccaaaat ttgaattatt ccgggaatcg
     6601 aggacccagc tttcttgtac aaagtggttg ataacagcgg gttaattaac atcttttacc
     6661 catacgatgt tcctgactat gcgggctatc cctatgacgt cccggactat gcaggatcct
     6721 atccatatga cgttccagat tacgctgctc agtgcagcgc ttagagctcg aatttccccg
     6781 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     6841 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     6901 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     6961 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     7021 tgttactaga tcgggaatta gcttcatcaa cgcaagacat gcgcacgacc gtctgacagg
     7081 agaggaattt ccgacgagca cagaaaggac ttgctcttgg acgtaggcct atttctcagg
     7141 cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt atcagccgcc gccaactggg
     7201 agatgaggag gctttcttgg ggggcagtca gcagttcatt tcacaagaca gaggaacttg
     7261 taaggagatg cactgattta tcttggcgca aaccagcagg acgaattagt gggaatagcc
     7321 cgcgaatatc taagttatgc ctgtcggcat gagcagaaac ttccaattcg aaacagtttg
     7381 gagaggttgt ttttgggcat accttttgtt agtcagcctc tcgattgctc atcgtcatta
     7441 cacagtaccg aagtttgatc gatctagtaa catagatgac accgcgcgcg ataatttatc
     7501 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
     7561 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
     7621 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
     7681 taagaaactt tattgccaaa tgtttgaacg atctgcttcg acgcactcct tctttactcc
     7741 accatctcgt ccttattgaa aacgtgggta gcaccaaaac gaatcaagtc gctggaactg
     7801 aagttaccaa tcacgctgga tgatttgcca gttggattaa tcttgccttt ccccgcatga
     7861 ataatattga tgaatgcatg cgtgaggggt atttcgattt tggcaatagc tgcaattgcc
     7921 gcgacatcct ccaacgagca taattcttca gaaaaatagc gatgttccat gttgtcaggg
     7981 catgcatgat gcacgttatg aggtgacggt gctaggcagt attccctcaa agtttcatag
     8041 tcagtatcat attcatcatt gcattcctgc aagagagaat tgagacgcaa tccacacgct
     8101 gcggcaacct tccggcgttc gtggtctatt tgctcttgga cgttgcaaac gtaagtgttg
     8161 gatcccggtc ggcatctact ctattccttt gccctcggac gagtgctggg gcgtcggttt
     8221 ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg
     8281 atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc
     8341 gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca
     8401 atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg
     8461 aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg
     8521 acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg
     8581 ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg
     8641 gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg
     8701 gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa
     8761 tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc
     8821 tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg
     8881 ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa
     8941 taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc
     9001 gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc
     9061 cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc
     9121 gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac
     9181 gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct ctccaaatga aatgaacttc
     9241 cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
     9301 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     9361 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     9421 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     9481 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     9541 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     9601 tggagtagac gagagtgtcg tgctccacca tgttgacgga tctctaggac gcgtcgacgc
     9661 gtcgacgcgt cgacgcgtcg acgcgtccta gaagctaatt cactggccgt cgttttacaa
     9721 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9781 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9841 agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     9901 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     9961 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
    10021 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
    10081 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
    10141 gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
    10201 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
    10261 ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt tattaagttg
    10321 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
    10381 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
    10441 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
    10501 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
    10561 tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc ctcgcagaga
    10621 tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg tcgatcttga
    10681 gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc tgagtggcgc
    10741 tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct caccgaatgg
    10801 tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc tgatcgaccc
    10861 cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc
    10921 cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga
    10981 gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga
    11041 gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt
    11101 gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg
    11161 cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag
    11221 ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag
    11281 agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca
    11341 ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt gcgccgagga
    11401 tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc acgagcaata
    11461 aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc tgttcgggct
    11521 ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc tcctgtttga
    11581 agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca
    11641 accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca
    11701 tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg
    11761 ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct ctgtttatcg
    11821 gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc
    11881 agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc cagccggaac
    11941 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc
    12001 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt
    12061 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg
    12121 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta
    12181 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc
    12241 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag
    12301 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg
    12361 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag
    12421 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc
    12481 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt
    12541 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg
    12601 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc
    12661 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg
    12721 ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc
    12781 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga
    12841 cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg
    12901 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg
    12961 ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct
    13021 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc
    13081 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg
    13141 ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac
    13201 cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt
    13261 ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc
    13321 ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc
    13381 atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa tattttattt
    13441 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
    13501 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
    13561 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
    13621 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
    13681 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
    13741 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
    13801 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
    13861 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
    13921 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
    13981 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
    14041 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
    14101 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
    14161 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
    14221 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
    14281 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
    14341 ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg tgatgctgcc
    14401 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
    14461 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
    14521 acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt tcacttacac
    14581 cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg gcgttggatg
    14641 ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc acttaaaaaa
    14701 ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg
    14761 gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg gctgttttac
    14821 gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactatggcg
    14881 acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg gatgacggat
    14941 ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag caagcgatat
    15001 acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg gctgggacgg
    15061 aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg gcattatctg
    15121 aacataaaac actatcaata agttggagtc attacccaat tatgatagaa tttacaagct
    15181 ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg ctttgcccat
    15241 tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc cttacatacg
    15301 gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat attcaaggca
    15361 atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat atggcgcttc
    15421 atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa tcttacctat
    15481 cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc acccgcgacc
    15541 actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc cgtgaatgcc
    15601 ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact gcccggcacc
    15661 tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg cgtcgcgctc
    15721 gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac tgccagcgct
    15781 gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg
    15841 cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg
    15901 tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc
    15961 aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga
    16021 ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact
    16081 ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg
    16141 tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta
    16201 aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg
    16261 agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc
    16321 cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc
    16381 gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc
    16441 cgccagccca gcggcgaggg caaccagccc ggtgagcgtc ggaaaggcgc
//
