LOCUS       sequence-246935-        2631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression vector for Escherichia coli. BglBrick 
            compatible..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Jervis AJ, Carbonell P, Taylor S, Sung R, Dunstan MS, Robinson CJ, 
            Breitling R, Takano E, Scrutton NS
  TITLE     SelProm: A Queryable and Predictive Expression Vector Selection Tool
            for Escherichia coli.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00399.
  PUBMED    30870592
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00399."
COMMENT     Sequence Label: pBbA11c-RFP
FEATURES             Location/Qualifiers
     source          1..2631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..41
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     promoter        7..41
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     promoter        7..41
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     CDS             86..763
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      796..867
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      883..910
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(938..955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1072..1617)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1152..1171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1731..1825)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1846..2505)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2420..2439
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2506..2608)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcttta cggctagctc agtcctaggt attatgctag caggtttaat cgaatcaaaa
       61 gatcttttaa gaaggagata tacatatggc gagtagcgaa gacgttatca aagagttcat
      121 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
      181 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
      241 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
      301 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
      361 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
      421 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
      481 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
      541 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
      601 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
      661 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
      721 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taaggatcca aactcgagta
      781 aggatctcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      841 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
      901 cctttctgcg tttataccta gggatatatt ccgcttcctc gctcactgac tcgctacgct
      961 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag
     1021 atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag
     1081 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
     1141 gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt
     1201 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac
     1261 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc
     1321 ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa
     1381 gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg
     1441 aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca
     1501 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc
     1561 aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa
     1621 gatcatctta ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat
     1681 gtagcacctg aagtcagccc catacgatat aagttgttac tagtgcttgg attctcacca
     1741 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1801 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     1861 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     1921 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1981 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     2041 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     2101 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     2161 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     2221 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     2281 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     2341 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     2401 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     2461 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     2521 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     2581 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccagatat c
//
