LOCUS       sequence-246938-       14966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14966)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 14966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX079
FEATURES             Location/Qualifiers
     source          1..14966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5112..5136)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5165..5254
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5288..5540
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(5969..6221)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6689..7714)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6689..7714)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7724..8068)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8178..8195)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8178..8194)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8187..8209)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8334..8353)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8544..8565
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8812..8836
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10432..11580)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11879..12673)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12865..13632
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14325..14956
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgggctc
     4621 ggtttcttct cccgagggcc agcaacagac agctggaaat agccaggttt atggaacttc
     4681 acgatccagc gatgcagtaa atatgggatc ccaaggagtt acttccccat atcgtccagc
     4741 ttctgttcct atggggtact atgcattgca gggggagaat gtcttccctg aacgacctgg
     4801 gcagcctgaa tgccagtttt acatgaagac tggagactgc aagtttggtg ctgtttgtag
     4861 attccatcat ccaagggaaa ggctgatacc tcctcctgac tgcatgttaa gtcccattgg
     4921 acttccttta cgccctggag aaccgttgtg tattttctat tccagatatg ggatctgcaa
     4981 atttggccca agttgcaagt ttgatcaccc aatgacggta ttcacttata gcatctcagc
     5041 atcatctcct actgatgctc ctgcagttca gcgtctattg ggttcatcat caggaaccag
     5101 tgctcccggg gacccagctt tcttgtacaa agtggttgat aacagcgggt taattaacat
     5161 cttttaccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc cggactatgc
     5221 aggatcctat ccatatgacg ttccagatta cgctgctcag tgcagcgctt agagctcgaa
     5281 tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     5341 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     5401 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     5461 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     5521 gtcatctatg ttactagatc gggaattagc ttcatcaacg caagacatgc gcacgaccgt
     5581 ctgacaggag aggaatttcc gacgagcaca gaaaggactt gctcttggac gtaggcctat
     5641 ttctcaggca catgtatcaa gtgttcggac gtgggttttc gatggtgtat cagccgccgc
     5701 caactgggag atgaggaggc tttcttgggg ggcagtcagc agttcatttc acaagacaga
     5761 ggaacttgta aggagatgca ctgatttatc ttggcgcaaa ccagcaggac gaattagtgg
     5821 gaatagcccg cgaatatcta agttatgcct gtcggcatga gcagaaactt ccaattcgaa
     5881 acagtttgga gaggttgttt ttgggcatac cttttgttag tcagcctctc gattgctcat
     5941 cgtcattaca cagtaccgaa gtttgatcga tctagtaaca tagatgacac cgcgcgcgat
     6001 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     6061 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     6121 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     6181 ttcaatctta agaaacttta ttgccaaatg tttgaacgat ctgcttcgac gcactccttc
     6241 tttactccac catctcgtcc ttattgaaaa cgtgggtagc accaaaacga atcaagtcgc
     6301 tggaactgaa gttaccaatc acgctggatg atttgccagt tggattaatc ttgcctttcc
     6361 ccgcatgaat aatattgatg aatgcatgcg tgaggggtat ttcgattttg gcaatagctg
     6421 caattgccgc gacatcctcc aacgagcata attcttcaga aaaatagcga tgttccatgt
     6481 tgtcagggca tgcatgatgc acgttatgag gtgacggtgc taggcagtat tccctcaaag
     6541 tttcatagtc agtatcatat tcatcattgc attcctgcaa gagagaattg agacgcaatc
     6601 cacacgctgc ggcaaccttc cggcgttcgt ggtctatttg ctcttggacg ttgcaaacgt
     6661 aagtgttgga tcccggtcgg catctactct attcctttgc cctcggacga gtgctggggc
     6721 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc
     6781 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc
     6841 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
     6901 caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc
     6961 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga
     7021 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg
     7081 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc
     7141 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg
     7201 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc
     7261 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc
     7321 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca
     7381 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg
     7441 agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga
     7501 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc
     7561 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt
     7621 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
     7681 cgacagacgt cgcggtgagt tcaggctttt tcatatcggg gtcgtcctct ccaaatgaaa
     7741 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
     7801 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     7861 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     7921 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
     7981 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
     8041 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
     8101 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttgacggatc tctaggacgc
     8161 gtcctagaag ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     8221 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8281 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt
     8341 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8401 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8461 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8521 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8581 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
     8641 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     8701 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccacccca
     8761 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     8821 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     8881 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     8941 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     9001 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     9061 gctgcctgtg atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc
     9121 atttctcgct taaccgtgac aggctgtcga tcttgagaac tatgccgaca taataggaaa
     9181 tcgctggata aagccgctga ggaagctgag tggcgctatt tctttagaag tgaacgttga
     9241 cgatatcaac tcccctatcc attgctcacc gaatggtaca ggtcggggac ccgaagttcc
     9301 gactgtcggc ctgatgcatc cccggctgat cgaccccaga tctggggctg agaaagccca
     9361 gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta
     9421 aacccgctcc gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg
     9481 ggattggcgg atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt
     9541 gccaggcggt aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc
     9601 cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg
     9661 cgtttggtgt caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg
     9721 ccatcaatcg tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct
     9781 gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca
     9841 agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc
     9901 ccgttggaat ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca
     9961 gcataccggc gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg
    10021 ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc
    10081 cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg
    10141 gctttttctc ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg
    10201 acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct
    10261 taatcacaat tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg
    10321 tcccgagcga tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc
    10381 cagtaaagcg ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt
    10441 gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc
    10501 ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca
    10561 catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc
    10621 gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta
    10681 gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt
    10741 tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc
    10801 aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc
    10861 cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt
    10921 gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac
    10981 cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt
    11041 gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt
    11101 gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc
    11161 aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc
    11221 ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt
    11281 ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg
    11341 ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag
    11401 ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg
    11461 gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc
    11521 ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat
    11581 tcaccctcct tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt
    11641 tgacccgcct ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc
    11701 gtagaccgtc tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac
    11761 cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat
    11821 gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggtact
    11881 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
    11941 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
    12001 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
    12061 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
    12121 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
    12181 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
    12241 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
    12301 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
    12361 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
    12421 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
    12481 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
    12541 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
    12601 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
    12661 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
    12721 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
    12781 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
    12841 gccgattttg aaaccacaat tatgggtgat gctgccaact tactgattta gtgtatgatg
    12901 gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac
    12961 tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca
    13021 ctgccgtaaa acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca
    13081 gaaaatcatt gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg
    13141 cgttggcctc aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg
    13201 cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat
    13261 gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg
    13321 gttgttgcgc acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg
    13381 ctgtcaccct ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc
    13441 ctgaagggaa agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac
    13501 ctgaatctga ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg
    13561 gtggagctgc atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt
    13621 ggagtcatta cccaattatg atagaattta caagctataa ggttattgtc ctgggtttca
    13681 agcattagtc catgcaagtt tttatgcttt gcccattcta tagatatatt gataagcgcg
    13741 ctgcctatgc cttgccccct gaaatcctta catacggcga tatcttctat ataaaagata
    13801 tattatctta tcagtattgt caatatattc aaggcaatct gcctcctcat cctcttcatc
    13861 ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag agtaattctg taaaggtcca
    13921 attctcgttt tcatacctcg gtataatctt acctatcacc tcaaatggtt cgctgggttt
    13981 atcgcacccc cgaacacgag cacggcaccc gcgaccacta tgccaagaat gcccaaggta
    14041 aaaattgccg gccccgccat gaagtccgtg aatgccccga cggccgaagt gaagggcagg
    14101 ccgccaccca ggccgccgcc ctcactgccc ggcacctggt cgctgaatgt cgatgccagc
    14161 acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc tgatcgccca tcccgttact
    14221 gccccgatcc cggcaatggc aaggactgcc agcgctgcca tttttggggt gaggccgttc
    14281 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt
    14341 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg
    14401 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg
    14461 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc
    14521 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg
    14581 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc
    14641 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg
    14701 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc
    14761 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc
    14821 caagttttcc gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga
    14881 cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac
    14941 cagcccggtg agcgtcggaa aggcgc
//
