LOCUS       sequence-24694-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Kim DS, Matsuda T, Cepko CL
  TITLE     A core paired-type and POU homeodomain-containing transcription 
            factor program drives retinal bipolar cell gene expression.
  JOURNAL   J Neurosci. 2008 Jul 30. 28(31):7748-64.
  PUBMED    18667607
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Jul 30. 28(31):7748-64."
COMMENT     Sequence Label: SV40 basal promoter-GFP-IRES-AP
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..224
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      75..210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      259..268
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(265..267,268..270,271..729)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLE*NYNSHNVYIMA"
     primer_bind     complement(310..331)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(571..590)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 anggngacat tgctgagatc tgcgatctgc atctcaatta gtcagcaacc atagtcccgc
       61 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatc
      121 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
      181 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt ggtaccgcgg
      241 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
      301 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      361 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      421 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      481 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      541 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      601 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      661 gaggacggca acatcctggg gcacaagctg gagtaaaact acaacagcca caacgtctat
      721 atcatggccg acaagcanaa aacggcatca ggnnaanttc agatccccaa aaatcgagan
      781 gcggcgtgaa ctcgcnacat acanaaaanc cnttggaagg ccgtgtgtgc aaaacaaact
      841 gacc
//
