LOCUS       sequence-246940-       15127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15127)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX081
FEATURES             Location/Qualifiers
     source          1..15127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5241..5265)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5294..5383
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5417..5669
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6098..6350)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6818..7843)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6818..7843)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7853..8197)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8339..8356)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8339..8355)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8348..8370)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8495..8514)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8705..8726
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8973..8997
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10593..11741)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12040..12834)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLQGIKPDWEKIKYYILLDELF"
     mobile_element  13026..13793
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14486..15117
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg agaacccttt gtgatgtttg
     4621 tgaaagtgct gctgctatac tattttgtgc tgctgatgag gcttgtcttt gccatgcttg
     4681 tgatgagaag gtccatatgt gcaacaagct tgcaagtcga catgtacggg ttgggcttgc
     4741 tgaccccagt aaaatccaac gttgtgacat atgtgaaaat gcacctgctt tcttttattg
     4801 tgaaatcgat ggaagttccc tttgtttgca atgtgatatg attgtccatg ttggaggtaa
     4861 aagaacccat gaaagatatc tccttataag gcagagaatc gagtttccag gggataaatt
     4921 gggcccttca aatgaactag gactgccacc tactgaacaa ggtgatgtaa ggagggagcc
     4981 ggcacagccc tttaagctcc ctatgataga aaaccaccaa tcaaacaggg aaactgctaa
     5041 gctagggcta gaaaataatg tgaatagtag tgtgaaaatg gagaatgagt tgattgacct
     5101 taattccaga cctcaaagga tgcacggtca atcgtcaaat aatcaggaac aaggaatggg
     5161 catgcttagt ggcagcaatc atgagtcagt cggcgttgtt cctgatggac ccttcaaaag
     5221 agagccagag aagcccgggg acccagcttt cttgtacaaa gtggttgata acagcgggtt
     5281 aattaacatc ttttacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc
     5341 ggactatgca ggatcctatc catatgacgt tccagattac gctgctcagt gcagcgctta
     5401 gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     5461 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     5521 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     5581 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     5641 gcgcgcggtg tcatctatgt tactagatcg ggaattagct tcatcaacgc aagacatgcg
     5701 cacgaccgtc tgacaggaga ggaatttccg acgagcacag aaaggacttg ctcttggacg
     5761 taggcctatt tctcaggcac atgtatcaag tgttcggacg tgggttttcg atggtgtatc
     5821 agccgccgcc aactgggaga tgaggaggct ttcttggggg gcagtcagca gttcatttca
     5881 caagacagag gaacttgtaa ggagatgcac tgatttatct tggcgcaaac cagcaggacg
     5941 aattagtggg aatagcccgc gaatatctaa gttatgcctg tcggcatgag cagaaacttc
     6001 caattcgaaa cagtttggag aggttgtttt tgggcatacc ttttgttagt cagcctctcg
     6061 attgctcatc gtcattacac agtaccgaag tttgatcgat ctagtaacat agatgacacc
     6121 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     6181 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     6241 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
     6301 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttcgacg
     6361 cactccttct ttactccacc atctcgtcct tattgaaaac gtgggtagca ccaaaacgaa
     6421 tcaagtcgct ggaactgaag ttaccaatca cgctggatga tttgccagtt ggattaatct
     6481 tgcctttccc cgcatgaata atattgatga atgcatgcgt gaggggtatt tcgattttgg
     6541 caatagctgc aattgccgcg acatcctcca acgagcataa ttcttcagaa aaatagcgat
     6601 gttccatgtt gtcagggcat gcatgatgca cgttatgagg tgacggtgct aggcagtatt
     6661 ccctcaaagt ttcatagtca gtatcatatt catcattgca ttcctgcaag agagaattga
     6721 gacgcaatcc acacgctgcg gcaaccttcc ggcgttcgtg gtctatttgc tcttggacgt
     6781 tgcaaacgta agtgttggat cccggtcggc atctactcta ttcctttgcc ctcggacgag
     6841 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
     6901 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
     6961 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
     7021 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
     7081 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
     7141 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
     7201 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
     7261 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
     7321 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
     7381 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
     7441 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
     7501 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
     7561 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
     7621 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
     7681 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
     7741 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
     7801 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatcgggg tcgtcctctc
     7861 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
     7921 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     7981 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     8041 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     8101 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     8161 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     8221 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tgacggatct
     8281 ctaggacgcg tcgacgcgtc gacgcgtcga cgcgtcgacg cgtcctagaa gctaattcac
     8341 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     8401 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     8461 cttcccaaca gttgcgcagc ctgaatggcg cccgctcctt tcgctttctt cccttccttt
     8521 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8581 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     8641 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8701 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     8761 gatttataag ggattttgcc gatttcggaa ccaccatcaa acaggatttt cgcctgctgg
     8821 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     8881 agctgttgcc cgtctcactg gtgaaaagaa aaaccacccc agtacattaa aaacgtccgc
     8941 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     9001 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     9061 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     9121 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     9181 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaaatat
     9241 catctccctc gcagagatcc gaattatcag ccttcttatt catttctcgc ttaaccgtga
     9301 caggctgtcg atcttgagaa ctatgccgac ataataggaa atcgctggat aaagccgctg
     9361 aggaagctga gtggcgctat ttctttagaa gtgaacgttg acgatatcaa ctcccctatc
     9421 cattgctcac cgaatggtac aggtcgggga cccgaagttc cgactgtcgg cctgatgcat
     9481 ccccggctga tcgaccccag atctggggct gagaaagccc agtaaggaaa caactgtagg
     9541 ttcgagtcgc gagatccccc ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc
     9601 gagccacgcc aggccgagaa cattggttcc tgtaggcatc gggattggcg gatcaaacac
     9661 taaagctact ggaacgagca gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag
     9721 cagaggcacg ggaggttgcc acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc
     9781 ccccgccagg cccgctgcga cgccgacagg atctagcgct gcgtttggtg tcaacaccaa
     9841 cagcgccacg cccgcagttc cgcaaatagc ccccaggacc gccatcaatc gtatcgggct
     9901 acctagcaga gcggcagaga tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc
     9961 cgcgaccccg cccggcaggc ggtagaccga aataaacaac aagctccaga atagcgaaat
    10021 attaagtgcg ccgaggatga agatgcgcat ccaccagatt cccgttggaa tctgtcggac
    10081 gatcatcacg agcaataaac ccgccggcaa cgcccgcagc agcataccgg cgacccctcg
    10141 gcctcgctgt tcgggctcca cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg
    10201 gccgtcctcc tgtttgaaga ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa
    10261 tgccacggca tctcgcaacc gttcagcgaa cgcctccatg ggctttttct cctcgtgctc
    10321 gtaaacggac ccgaacatct ctggagcttt cttcagggcc gacaatcgga tctcgcggaa
    10381 atcctgcacg tcggccgctc caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt
    10441 taatcctctg tttatcggca gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg
    10501 aagcaagtgc gtcgagcagt gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct
    10561 gaacccccag ccggaactga ccccacaagg ccctagcgtt tgcaatgcac caggtcatca
    10621 ttgacccagg cgtgttccac caggccgctg cctcgcaact cttcgcaggc ttcgccgacc
    10681 tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc
    10741 agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc
    10801 ggcgacagct tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc
    10861 acgacgattt cctcgtcgat caggacctgg caacgggacg ttttcttgcc acggtccagg
    10921 acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag
    10981 cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta ataccggcca
    11041 ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg
    11101 ataggggtgc gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc
    11161 cgcagctcga cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg
    11221 ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc
    11281 gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa caaggaaagc
    11341 tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg
    11401 acctgttttg ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat agttcctcgc
    11461 gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct gttcgagacg acgcgaacgc
    11521 tccacggcgg ccgatggcgc gggcagggca gggggagcca gttgcacgct gtcgcgctcg
    11581 atcttggccg tagcttgctg gaccatcgag ccgacggact ggaaggtttc gcggggcgca
    11641 cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc
    11701 agttcttgcc tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt
    11761 gccccgactc acgccggggc aatgtgccct tattcctgat ttgacccgcc tggtgccttg
    11821 gtgtccagat aatccacctt atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc
    11881 ttctcgtact tggtattccg aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa
    11941 tacttgccgt gggcctcggc ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc
    12001 tcctgcttgt cgccggcatc gttgcgccac atctaggtac taaaacaatt catccagtaa
    12061 aatataatat tttattttct cccaatcagg cttgatcccc tgtaagtcaa aaaatagctc
    12121 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
    12181 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
    12241 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
    12301 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
    12361 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
    12421 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
    12481 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
    12541 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
    12601 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
    12661 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
    12721 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
    12781 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
    12841 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
    12901 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
    12961 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccacaa
    13021 ttatgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca
    13081 gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac
    13141 ggcaaaagca ccgccggaca tcagcgctat ctctgctctc actgccgtaa aacatggcaa
    13201 ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc
    13261 atgaatggcg ttggatgccg ggcaacagcc cgcattatgg gcgttggcct caacacgatt
    13321 ttacgtcact taaaaaactc aggccgcagt cggtaacctc gcgcatacag ccgggcagtg
    13381 acgtcatcgt ctgcgcggaa atggacgaac agtggggcta tgtcggggct aaatcgcgcc
    13441 agcgctggct gttttacgcg tatgacagtc tccggaagac ggttgttgcg cacgtattcg
    13501 gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg
    13561 tgatatggat gacggatggc tggccgctgt atgaatcccg cctgaaggga aagctgcacg
    13621 taatcagcaa gcgatatacg cagcgaattg agcggcataa cctgaatctg aggcagcacc
    13681 tggcacggct gggacggaag tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag
    13741 tcatcgggca ttatctgaac ataaaacact atcaataagt tggagtcatt acccaattat
    13801 gatagaattt acaagctata aggttattgt cctgggtttc aagcattagt ccatgcaagt
    13861 ttttatgctt tgcccattct atagatatat tgataagcgc gctgcctatg ccttgccccc
    13921 tgaaatcctt acatacggcg atatcttcta tataaaagat atattatctt atcagtattg
    13981 tcaatatatt caaggcaatc tgcctcctca tcctcttcat cctcttcgtc ttggtagctt
    14041 tttaaatatg gcgcttcata gagtaattct gtaaaggtcc aattctcgtt ttcatacctc
    14101 ggtataatct tacctatcac ctcaaatggt tcgctgggtt tatcgcaccc ccgaacacga
    14161 gcacggcacc cgcgaccact atgccaagaa tgcccaaggt aaaaattgcc ggccccgcca
    14221 tgaagtccgt gaatgccccg acggccgaag tgaagggcag gccgccaccc aggccgccgc
    14281 cctcactgcc cggcacctgg tcgctgaatg tcgatgccag cacctgcggc acgtcaatgc
    14341 ttccgggcgt cgcgctcggg ctgatcgccc atcccgttac tgccccgatc ccggcaatgg
    14401 caaggactgc cagcgctgcc atttttgggg tgaggccgtt cgcggccgag gggcgcagcc
    14461 cctgggggga tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc
    14521 cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata
    14581 aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa
    14641 cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc
    14701 cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta
    14761 gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc
    14821 tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac
    14881 gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc
    14941 cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat
    15001 ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt
    15061 gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt gagcgtcgga
    15121 aaggcgc
//
