LOCUS       sequence-246941-       16879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16879)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX082
FEATURES             Location/Qualifiers
     source          1..16879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6993..7017)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7046..7135
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      7169..7421
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7850..8102)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8570..9595)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8570..9595)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9605..9949)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10091..10108)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10091..10107)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10100..10122)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10247..10266)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10457..10478
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10725..10749
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12345..13493)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13792..14586)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14778..15545
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16238..16869
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgtccga
     4621 cccacagccc tcctcgatcc aacacgatac aaccaatacc cactcggaaa acaaagctga
     4681 aaacaactat aacaacagta acagtaatag taatgctaat aacactagta gtaataacaa
     4741 tatagttgtc aggagagaga ggccggcgag ggagtgtacg aaaagggcag cggcgaggtt
     4801 acaagcggca gcagcggcgg aggcggaggc tgctgctgca gtgaggaaga aaaagtctgg
     4861 agttaggaaa gaaaggttag cggctaggtt attgagggag gaggaggatg aggaggagaa
     4921 gtttctagaa ggtgataatg atgatgagga aggcgaagaa gatgggtcgc cgtcttcgtc
     4981 gaggcaacaa tgcagcaaga ttgtcacaca gcttgtggga gagcccgagc cttcgcagtt
     5041 gccacggtgg aacctgcggt ccatgtggca gttagcttcc atattgaatt tcttgaatgt
     5101 ttttaggcct ttattgaata ttagagtgga gttctcagcg gaggaatttg aaacagcatt
     5161 aattactcca aatgacacac ttggtgatat acatatgcct ttgctaaagg caattccacc
     5221 agttacccga gtggcacttg gacacaatac atggattacg gttttgtgca ggaaattaag
     5281 agattggtgg cattgggttg ctgaagggga actacctata gttgcgtcac atggggctga
     5341 aattgaagct tataatgcac tagatcctgc agtccgggtg gtgattttga aagcactatg
     5401 tgatattcgt gttgagcaag aagatattcg taactacata gacgactcct tgaaacaagg
     5461 tgttcgcctt tcacttttcc gaaaagagcg tattggcggt gattctcatg gaatttcgta
     5521 ctggtatgag gacgatccta tcattgggca tcgattatac cgagagatta gaaaggttga
     5581 agtcaagaaa ggaaagggca agaatgttcc ggcaatacct cactcatgtt accagtggga
     5641 aactgttgca actaatttcg atgaattcgg ggatgtttct gagaagctat tcacgagtaa
     5701 aaatagaaca gaggcttctg ttggaaagaa gttgaaaaat gacatgcttc ctgaggttga
     5761 gagggtccac aagaggaaag agaaattgct gaagaaacaa catagacaag cattgatgct
     5821 tgataacatg ctaaatatgg atggtcttgg tggtggacgc gccttgcggg gtaggaagcc
     5881 tgtgacctac acatttgatg attacgatcg gtcaattaat gaggctatca agctaaccaa
     5941 gaagaatcaa ccatccccag aacctacttt gaggagagaa gctgctacga agtatgaaac
     6001 ttctataaat ggtagattgg gtagtcctcc acacttgtct caacatgtgt ctttcagagt
     6061 gcgttctcct aaatcacctg aatacgatga atatgacgaa gacaatgaag atgaactatt
     6121 agatcgaagc aatcgtacga ggcagagacc ccaacgctat tctgcatcag agtttgttga
     6181 agcaatatct gaaaatgagg cagatgttga cagtgatgat gatataatgg gtgaagcagt
     6241 atatgatgaa gaatatttaa ggaaacgaaa acatcggaag gtgtcaagta gctcggaagg
     6301 agatgatgag taccactggg atgaagaaaa tcctgaagat gaagaagagg aggatgaaga
     6361 tgaagcagat tccctaagtg ccagtgacga gagcgatgag ccacggagat ttaagaaact
     6421 gcctggtcgt acaagaagag aaaccaagtt gaggtcagtg ggcaaccttc agtcaggttt
     6481 aagacgcagt aacagggcaa ccagaaaccg tattaattat agccatttag agctgtcaga
     6541 atctgagccc gagtcaatga aacctgagaa gtcaaatgga tccgaagagc aatatgatgc
     6601 aattgacagg gcaaccagaa accgtatcaa ttatagccgt ttagaactgt cagaatctga
     6661 gcccgagtca atgaaacctg agaagtcaaa tggatccgaa gagcaatatt atgcaagtga
     6721 taatgctgag ttctcgatgg gaagtgaaaa cacagaggaa aactatgaga atcaggaggt
     6781 gaaagctgag caacctgttg atgtaccatc tgagatggca acaacagatc aaaacaatgt
     6841 accaaagaga acaagcagtc caggtcaaga tgatgttgaa agtggaggga aaaggcgctt
     6901 tcttgatctt aatgagcttg ctccatgttc tggatttgac gatggtccca attcagtgat
     6961 gaaagatgat ggtccagatg actttcccgg ggacccagct ttcttgtaca aagtggttga
     7021 taacagcggg ttaattaaca tcttttaccc atacgatgtt cctgactatg cgggctatcc
     7081 ctatgacgtc ccggactatg caggatccta tccatatgac gttccagatt acgctgctca
     7141 gtgcagcgct tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     7201 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     7261 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     7321 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     7381 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattag cttcatcaac
     7441 gcaagacatg cgcacgaccg tctgacagga gaggaatttc cgacgagcac agaaaggact
     7501 tgctcttgga cgtaggccta tttctcaggc acatgtatca agtgttcgga cgtgggtttt
     7561 cgatggtgta tcagccgccg ccaactggga gatgaggagg ctttcttggg gggcagtcag
     7621 cagttcattt cacaagacag aggaacttgt aaggagatgc actgatttat cttggcgcaa
     7681 accagcagga cgaattagtg ggaatagccc gcgaatatct aagttatgcc tgtcggcatg
     7741 agcagaaact tccaattcga aacagtttgg agaggttgtt tttgggcata ccttttgtta
     7801 gtcagcctct cgattgctca tcgtcattac acagtaccga agtttgatcg atctagtaac
     7861 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc
     7921 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc
     7981 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc
     8041 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
     8101 tctgcttcga cgcactcctt ctttactcca ccatctcgtc cttattgaaa acgtgggtag
     8161 caccaaaacg aatcaagtcg ctggaactga agttaccaat cacgctggat gatttgccag
     8221 ttggattaat cttgcctttc cccgcatgaa taatattgat gaatgcatgc gtgaggggta
     8281 tttcgatttt ggcaatagct gcaattgccg cgacatcctc caacgagcat aattcttcag
     8341 aaaaatagcg atgttccatg ttgtcagggc atgcatgatg cacgttatga ggtgacggtg
     8401 ctaggcagta ttccctcaaa gtttcatagt cagtatcata ttcatcattg cattcctgca
     8461 agagagaatt gagacgcaat ccacacgctg cggcaacctt ccggcgttcg tggtctattt
     8521 gctcttggac gttgcaaacg taagtgttgg atcccggtcg gcatctactc tattcctttg
     8581 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
     8641 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
     8701 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
     8761 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg
     8821 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
     8881 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
     8941 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
     9001 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
     9061 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
     9121 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
     9181 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
     9241 tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
     9301 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt
     9361 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
     9421 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
     9481 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
     9541 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatcgg
     9601 ggtcgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
     9661 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
     9721 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     9781 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     9841 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     9901 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     9961 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
    10021 gttgacggat ctctaggacg cgtcgacgcg tcgacgcgtc gacgcgtcga cgcgtcctag
    10081 aagctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
    10141 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
    10201 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc
    10261 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    10321 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
    10381 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    10441 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    10501 ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt
    10561 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
    10621 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt
    10681 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
    10741 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
    10801 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
    10861 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
    10921 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
    10981 gtgatcaaat atcatctccc tcgcagagat ccgaattatc agccttctta ttcatttctc
    11041 gcttaaccgt gacaggctgt cgatcttgag aactatgccg acataatagg aaatcgctgg
    11101 ataaagccgc tgaggaagct gagtggcgct atttctttag aagtgaacgt tgacgatatc
    11161 aactccccta tccattgctc accgaatggt acaggtcggg gacccgaagt tccgactgtc
    11221 ggcctgatgc atccccggct gatcgacccc agatctgggg ctgagaaagc ccagtaagga
    11281 aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg ttaaacccgc
    11341 tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca tcgggattgg
    11401 cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca gttgccaggc
    11461 ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg ttccgaacgc
    11521 catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg ctgcgtttgg
    11581 tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga ccgccatcaa
    11641 tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg gctgcacagc
    11701 gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca acaagctcca
    11761 gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga ttcccgttgg
    11821 aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca gcagcatacc
    11881 ggcgacccct cggcctcgct gttcgggctc cacgaaaacg ccggacagat gcgccttgtg
    11941 agcgtccttg gggccgtcct cctgtttgaa gaccgacagc ccaatgatct cgccgtcgat
    12001 gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg aacgcctcca tgggcttttt
    12061 ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct ttcttcaggg ccgacaatcg
    12121 gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt cgaatctgag ccttaatcac
    12181 aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag
    12241 cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa
    12301 gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc
    12361 accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag
    12421 gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg
    12481 cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc
    12541 cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg
    12601 ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg
    12661 ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg
    12721 tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg
    12781 taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg
    12841 atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg
    12901 tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg
    12961 aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg
    13021 gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg
    13081 aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc
    13141 ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc
    13201 atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga
    13261 cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg
    13321 ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt
    13381 tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac
    13441 cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct
    13501 ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg
    13561 cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc
    13621 gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac
    13681 cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag
    13741 aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctaggt actaaaacaa
    13801 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
    13861 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
    13921 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
    13981 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
    14041 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
    14101 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
    14161 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
    14221 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
    14281 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
    14341 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
    14401 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
    14461 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
    14521 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
    14581 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    14641 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    14701 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    14761 ttgaaaccac aattatgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt
    14821 tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg
    14881 gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc tcactgccgt
    14941 aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc
    15001 attgatatgg ccatgaatgg cgttggatgc cgggcaacag cccgcattat gggcgttggc
    15061 ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
    15121 agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg
    15181 ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg
    15241 cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac
    15301 cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg
    15361 gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc
    15421 tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc
    15481 tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca
    15541 ttacccaatt atgatagaat ttacaagcta taaggttatt gtcctgggtt tcaagcatta
    15601 gtccatgcaa gtttttatgc tttgcccatt ctatagatat attgataagc gcgctgccta
    15661 tgccttgccc cctgaaatcc ttacatacgg cgatatcttc tatataaaag atatattatc
    15721 ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct catcctcttc atcctcttcg
    15781 tcttggtagc tttttaaata tggcgcttca tagagtaatt ctgtaaaggt ccaattctcg
    15841 ttttcatacc tcggtataat cttacctatc acctcaaatg gttcgctggg tttatcgcac
    15901 ccccgaacac gagcacggca cccgcgacca ctatgccaag aatgcccaag gtaaaaattg
    15961 ccggccccgc catgaagtcc gtgaatgccc cgacggccga agtgaagggc aggccgccac
    16021 ccaggccgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg
    16081 gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga
    16141 tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg
    16201 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa
    16261 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa
    16321 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa
    16381 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc
    16441 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct
    16501 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt
    16561 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct
    16621 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg
    16681 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt
    16741 tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt
    16801 tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg
    16861 gtgagcgtcg gaaaggcgc
//
