LOCUS       sequence-246945-       16502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16502)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX084
FEATURES             Location/Qualifiers
     source          1..16502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6616..6640)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6669..6758
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6792..7044
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7473..7725)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8193..9218)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8193..9218)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9228..9572)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9714..9731)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9714..9730)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9723..9745)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9870..9889)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10080..10101
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10348..10372
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11968..13116)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13415..14209)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14401..15168
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15861..16492
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gttgtgaagt
     4621 tggttttgtc aatctgttgg tgttcaatgg caagtatgag tggcgacggt gccacgtgta
     4681 agtcggaagc tgcagataag tttccagtag ggttaagagt tcttgtggtt gatgacgaca
     4741 tcgtttgcct tagaattctg gagcaaatgc ttcgcaaatg caagtattct gttaccactt
     4801 gttcccaagc tgcggtggca ttaaacctac tacgggaaag gagggggtct tttgatgttg
     4861 tactgagtga tgttcatatg cctgatatgg atggttttaa acttcttgag cttgttgggt
     4921 tggaaatgga ccttccagta ataatgatgt caggtgatgg aagaaccaac ctcgtcatga
     4981 gaggagttca gcatggggct tgtgattatt tgattaagcc tatacgtgat gaggagctaa
     5041 agaatatctg gcagcatgtt gttaggaaaa gatgtagctt cagtaaagag cctgaatatt
     5101 ctggtagctt ggatgaaaat gatcggcata aacgaggaaa tgatgatgct gaatgtgctt
     5161 cttctgtaat tgaaggagca gatggagtgc taaaaccaca gaaaaagaaa agagatgcaa
     5221 aagaagaaga tgaaacggaa atggaaaatg atgacccaaa tactgcaaaa aagccacgtg
     5281 tagtctggtc agtggagctt catcagcaat ttgttagtgc tgtcaaccaa ctcgggattg
     5341 ataaggctgt acctaagaga atccttgaac tgatgaatgt tcctggtttg acaagagaaa
     5401 atgtggcaag ccatcttcag aaatttaggc tgtacttgaa gaggctaagt ggagtagctc
     5461 agcaacaggg tggcttcccc agtacctact gcgggccaat agaacagaat ccaaaacttg
     5521 gtttactagg gagatttgac attcaagcac tggcagcctc tggtcaaatt cctcctgaaa
     5581 ctttgacagc tcttcatgct gagcttttag gtcgatcgtc aggtaacctg gttttaccat
     5641 cagtagagca gccgcatctc ctacaagcat ccctgcagca agcaaagtgc gtacctgttg
     5701 atcatgttgt ggcctatggt cagcctctga tgaaatgccc tcccagcatt actaatccta
     5761 aacttctttc tcaacctatg atgtctgctg aggatgttca ttcaggattt ggatcccgga
     5821 gagcaaaaaa tatatgtatg gttcctagca gcaatctcac tgggctagct gctccagaca
     5881 gcaacatgtt aatggcaatg atgcaacatc agcaacaatg gcaaaagcag cagcagctgg
     5941 aacagcaaca gaaacaatcc ggtctttctg aggccaacca ttcaattaat gtgaaacctt
     6001 cttgccttgt attgccttct caatcgccag gtaatttcca agtgggagat agtcctggct
     6061 ccgttagaca ggcctgcagt tcgagtaaat cttcagtaat tgattatagc gttctctcgc
     6121 cacagtcaaa taattcatca gctgtggtgc aagtgttaga cggggaactc aaaccagcat
     6181 gtggcttaaa caggctctcc actgcagctc ctttcactcc ttcagtctca tcatgctctg
     6241 ttcatgctga caatagtgtt ggtctgcagc ttcataactc tagctcggca tttggtgcat
     6301 ctaaacaact gccaggcttt gttcctaacc atctgggctt tccagtgtca tgtaatagta
     6361 aaccaagcca gattcttgac cagggaccta tgagaagtcc tggagttggt aaagctgccg
     6421 ccattcccag tcgttttgct gtagacgagt ctgattcgcc aatgtgcaat ttcagtactg
     6481 taaaagttta tactgaggac actaaagtta agcaggagcc taacatgaat atcatggaaa
     6541 attctaaagt tggaccagct ctttttcaaa acttaccatc tggtgatctt atgagtgtct
     6601 tcagcgaccc cggggaccca gctttcttgt acaaagtggt tgataacagc gggttaatta
     6661 acatctttta cccatacgat gttcctgact atgcgggcta tccctatgac gtcccggact
     6721 atgcaggatc ctatccatat gacgttccag attacgctgc tcagtgcagc gcttagagct
     6781 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     6841 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     6901 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     6961 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     7021 cggtgtcatc tatgttacta gatcgggaat tagcttcatc aacgcaagac atgcgcacga
     7081 ccgtctgaca ggagaggaat ttccgacgag cacagaaagg acttgctctt ggacgtaggc
     7141 ctatttctca ggcacatgta tcaagtgttc ggacgtgggt tttcgatggt gtatcagccg
     7201 ccgccaactg ggagatgagg aggctttctt ggggggcagt cagcagttca tttcacaaga
     7261 cagaggaact tgtaaggaga tgcactgatt tatcttggcg caaaccagca ggacgaatta
     7321 gtgggaatag cccgcgaata tctaagttat gcctgtcggc atgagcagaa acttccaatt
     7381 cgaaacagtt tggagaggtt gtttttgggc ataccttttg ttagtcagcc tctcgattgc
     7441 tcatcgtcat tacacagtac cgaagtttga tcgatctagt aacatagatg acaccgcgcg
     7501 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa
     7561 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
     7621 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
     7681 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatctgctt cgacgcactc
     7741 cttctttact ccaccatctc gtccttattg aaaacgtggg tagcaccaaa acgaatcaag
     7801 tcgctggaac tgaagttacc aatcacgctg gatgatttgc cagttggatt aatcttgcct
     7861 ttccccgcat gaataatatt gatgaatgca tgcgtgaggg gtatttcgat tttggcaata
     7921 gctgcaattg ccgcgacatc ctccaacgag cataattctt cagaaaaata gcgatgttcc
     7981 atgttgtcag ggcatgcatg atgcacgtta tgaggtgacg gtgctaggca gtattccctc
     8041 aaagtttcat agtcagtatc atattcatca ttgcattcct gcaagagaga attgagacgc
     8101 aatccacacg ctgcggcaac cttccggcgt tcgtggtcta tttgctcttg gacgttgcaa
     8161 acgtaagtgt tggatcccgg tcggcatcta ctctattcct ttgccctcgg acgagtgctg
     8221 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
     8281 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
     8341 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
     8401 tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga
     8461 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
     8521 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
     8581 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
     8641 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
     8701 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
     8761 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
     8821 aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc
     8881 tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
     8941 cgggagatgc aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc
     9001 gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg
     9061 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
     9121 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
     9181 ttctcgacag acgtcgcggt gagttcaggc tttttcatat cggggtcgtc ctctccaaat
     9241 gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat
     9301 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
     9361 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
     9421 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
     9481 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
     9541 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
     9601 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttgacg gatctctagg
     9661 acgcgtcgac gcgtcgacgc gtcgacgcgt cgacgcgtcc tagaagctaa ttcactggcc
     9721 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9781 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     9841 caacagttgc gcagcctgaa tggcgcccgc tcctttcgct ttcttccctt cctttctcgc
     9901 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     9961 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
    10021 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    10081 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt
    10141 ataagggatt ttgccgattt cggaaccacc atcaaacagg attttcgcct gctggggcaa
    10201 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg
    10261 ttgcccgtct cactggtgaa aagaaaaacc accccagtac attaaaaacg tccgcaatgt
    10321 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag
    10381 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
    10441 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
    10501 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
    10561 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca aatatcatct
    10621 ccctcgcaga gatccgaatt atcagccttc ttattcattt ctcgcttaac cgtgacaggc
    10681 tgtcgatctt gagaactatg ccgacataat aggaaatcgc tggataaagc cgctgaggaa
    10741 gctgagtggc gctatttctt tagaagtgaa cgttgacgat atcaactccc ctatccattg
    10801 ctcaccgaat ggtacaggtc ggggacccga agttccgact gtcggcctga tgcatccccg
    10861 gctgatcgac cccagatctg gggctgagaa agcccagtaa ggaaacaact gtaggttcga
    10921 gtcgcgagat cccccggaac caaaggaagt aggttaaacc cgctccgatc aggccgagcc
    10981 acgccaggcc gagaacattg gttcctgtag gcatcgggat tggcggatca aacactaaag
    11041 ctactggaac gagcagaagt cctccggccg ccagttgcca ggcggtaaag gtgagcagag
    11101 gcacgggagg ttgccacttg cgggtcagca cggttccgaa cgccatggaa accgcccccg
    11161 ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac accaacagcg
    11221 ccacgcccgc agttccgcaa atagccccca ggaccgccat caatcgtatc gggctaccta
    11281 gcagagcggc agagatgaac acgaccatca gcggctgcac agcgcctacc gtcgccgcga
    11341 ccccgcccgg caggcggtag accgaaataa acaacaagct ccagaatagc gaaatattaa
    11401 gtgcgccgag gatgaagatg cgcatccacc agattcccgt tggaatctgt cggacgatca
    11461 tcacgagcaa taaacccgcc ggcaacgccc gcagcagcat accggcgacc cctcggcctc
    11521 gctgttcggg ctccacgaaa acgccggaca gatgcgcctt gtgagcgtcc ttggggccgt
    11581 cctcctgttt gaagaccgac agcccaatga tctcgccgtc gatgtaggcg ccgaatgcca
    11641 cggcatctcg caaccgttca gcgaacgcct ccatgggctt tttctcctcg tgctcgtaaa
    11701 cggacccgaa catctctgga gctttcttca gggccgacaa tcggatctcg cggaaatcct
    11761 gcacgtcggc cgctccaagc cgtcgaatct gagccttaat cacaattgtc aattttaatc
    11821 ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca
    11881 agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc
    11941 cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac
    12001 ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc
    12061 gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt
    12121 gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga
    12181 cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac
    12241 gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg
    12301 gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat
    12361 cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat
    12421 cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg
    12481 ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag
    12541 ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg
    12601 cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc
    12661 gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat
    12721 ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg
    12781 ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc
    12841 gatggtcatc gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac
    12901 ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt
    12961 ggccgtagct tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat
    13021 gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc
    13081 ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc
    13141 gactcacgcc ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc
    13201 cagataatcc accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc
    13261 gtacttggta ttccgaatct tgccctgcac gaataccagc gaccccttgc ccaaatactt
    13321 gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg
    13381 cttgtcgccg gcatcgttgc gccacatcta ggtactaaaa caattcatcc agtaaaatat
    13441 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
    13501 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
    13561 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
    13621 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
    13681 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
    13741 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
    13801 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
    13861 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
    13921 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
    13981 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
    14041 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
    14101 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
    14161 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
    14221 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
    14281 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
    14341 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cacaattatg
    14401 ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc
    14461 ttctgtttct atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa
    14521 aagcaccgcc ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca
    14581 gttcacttac accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa
    14641 tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt ggcctcaaca cgattttacg
    14701 tcacttaaaa aactcaggcc gcagtcggta acctcgcgca tacagccggg cagtgacgtc
    14761 atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg gggctaaatc gcgccagcgc
    14821 tggctgtttt acgcgtatga cagtctccgg aagacggttg ttgcgcacgt attcggtgaa
    14881 cgcactatgg cgacgctggg gcgtcttatg agcctgctgt caccctttga cgtggtgata
    14941 tggatgacgg atggctggcc gctgtatgaa tcccgcctga agggaaagct gcacgtaatc
    15001 agcaagcgat atacgcagcg aattgagcgg cataacctga atctgaggca gcacctggca
    15061 cggctgggac ggaagtcgct gtcgttctca aaatcggtgg agctgcatga caaagtcatc
    15121 gggcattatc tgaacataaa acactatcaa taagttggag tcattaccca attatgatag
    15181 aatttacaag ctataaggtt attgtcctgg gtttcaagca ttagtccatg caagttttta
    15241 tgctttgccc attctataga tatattgata agcgcgctgc ctatgccttg ccccctgaaa
    15301 tccttacata cggcgatatc ttctatataa aagatatatt atcttatcag tattgtcaat
    15361 atattcaagg caatctgcct cctcatcctc ttcatcctct tcgtcttggt agctttttaa
    15421 atatggcgct tcatagagta attctgtaaa ggtccaattc tcgttttcat acctcggtat
    15481 aatcttacct atcacctcaa atggttcgct gggtttatcg cacccccgaa cacgagcacg
    15541 gcacccgcga ccactatgcc aagaatgccc aaggtaaaaa ttgccggccc cgccatgaag
    15601 tccgtgaatg ccccgacggc cgaagtgaag ggcaggccgc cacccaggcc gccgccctca
    15661 ctgcccggca cctggtcgct gaatgtcgat gccagcacct gcggcacgtc aatgcttccg
    15721 ggcgtcgcgc tcgggctgat cgcccatccc gttactgccc cgatcccggc aatggcaagg
    15781 actgccagcg ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg
    15841 ggggatggga ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt
    15901 cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat
    15961 tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt
    16021 gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca
    16081 tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc
    16141 gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg
    16201 gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc
    16261 cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca
    16321 agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca
    16381 acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg
    16441 gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc
    16501 gc
//
