LOCUS       sequence-24696-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Kim DS, Matsuda T, Cepko CL
  TITLE     A core paired-type and POU homeodomain-containing transcription 
            factor program drives retinal bipolar cell gene expression.
  JOURNAL   J Neurosci. 2008 Jul 30. 28(31):7748-64.
  PUBMED    18667607
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Jul 30. 28(31):7748-64."
COMMENT     Sequence Label: SV40 basal promoter-tdTomato-IRES-AP
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..224
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      75..210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      263..272
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(402..420)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ggnnganatt gctcgagatc tgcgatctgc atctcaatta gtcagcaacc atagtcccgc
       61 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatc
      121 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
      181 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt ggtaccgcgg
      241 gcccgggatc caccgggatc ccgccaccat ggtgagcaag ggcgaggagg tcatcaaaga
      301 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
      361 gggcgagggc gagggccgcc cctacnaggg cacccagacc gccaagctga aggtgaccaa
      421 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
      481 ggcgtacgtg aagcaccccg ccgacatccc cgattacaan aagctgtcct tccccgaggg
      541 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccanga
      601 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
      661 acagagnncn gc
//
