LOCUS       sequence-246965-       15410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15410)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX086
FEATURES             Location/Qualifiers
     source          1..15410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5524..5548)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5577..5666
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5700..5952
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6381..6633)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7101..8126)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7101..8126)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8136..8480)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8622..8639)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8622..8638)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8631..8653)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8778..8797)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8988..9009
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9256..9280
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10876..12024)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12323..13117)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13309..14076
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14769..15400
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtaaaaatga
     4621 gggaagagga ttcaaattgg tttgctaaat gggaagaaga attgccaaaa ccagaagagt
     4681 taatgccttt atctcaaacc cttatatctc ctgatcttgc tattgctttt gatatttcaa
     4741 accccactag cccaaataat gctcaaaaca aacagcagca gcagattcag attcctcaat
     4801 cctctcaaca gcagcccaat tcatcagctg aatttgagtc tactgaattg aatggattag
     4861 gaggtggtgg agatgaacca gctaggacaa taaaaaggcc taggcttgtg tggaccccac
     4921 agctacacaa gagatttgtg gatgcagttg ctcatttggg gatcaagaat gctgtcccaa
     4981 agactataat gcaactgatg agtgtcgatg gcctaacgcg cgaaaatgtt gctagtcatt
     5041 tgcaaaaata caggctttat ttgaaaagaa tgcaaggtct ttctaatggt ggtagtggtg
     5101 gaaacagtgg tcaatcttta tcaggggcag gtgttgattc tgcaatggac aatttgtttg
     5161 ctagttcgcc agtgccagca cattttttgc atccggccgg gaggggtaat cctgaccagt
     5221 tcttgccatt tgtgccagtg acatcaatgc agcatcataa tcatcatatg ggagtggttg
     5281 ttggacacca tcatcctcaa gttcaacagc agtataggca atttgggtca ccagcgaatg
     5341 gacattttga tcatcatcca tttttgtcta ggcaatcaca gcagcaggtt cagaggatgg
     5401 ggacatcagt gcataatggt agtcctgtgg tgtcacctta tgttgatgat gtggattctt
     5461 cagccgtgcc cgtcaatggg agaaaggtcc ttactctgtt cccaacaggg gatgatcccg
     5521 gggacccagc tttcttgtac aaagtggttg ataacagcgg gttaattaac atcttttacc
     5581 catacgatgt tcctgactat gcgggctatc cctatgacgt cccggactat gcaggatcct
     5641 atccatatga cgttccagat tacgctgctc agtgcagcgc ttagagctcg aatttccccg
     5701 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     5761 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     5821 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     5881 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     5941 tgttactaga tcgggaatta gcttcatcaa cgcaagacat gcgcacgacc gtctgacagg
     6001 agaggaattt ccgacgagca cagaaaggac ttgctcttgg acgtaggcct atttctcagg
     6061 cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt atcagccgcc gccaactggg
     6121 agatgaggag gctttcttgg ggggcagtca gcagttcatt tcacaagaca gaggaacttg
     6181 taaggagatg cactgattta tcttggcgca aaccagcagg acgaattagt gggaatagcc
     6241 cgcgaatatc taagttatgc ctgtcggcat gagcagaaac ttccaattcg aaacagtttg
     6301 gagaggttgt ttttgggcat accttttgtt agtcagcctc tcgattgctc atcgtcatta
     6361 cacagtaccg aagtttgatc gatctagtaa catagatgac accgcgcgcg ataatttatc
     6421 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
     6481 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
     6541 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
     6601 taagaaactt tattgccaaa tgtttgaacg atctgcttcg acgcactcct tctttactcc
     6661 accatctcgt ccttattgaa aacgtgggta gcaccaaaac gaatcaagtc gctggaactg
     6721 aagttaccaa tcacgctgga tgatttgcca gttggattaa tcttgccttt ccccgcatga
     6781 ataatattga tgaatgcatg cgtgaggggt atttcgattt tggcaatagc tgcaattgcc
     6841 gcgacatcct ccaacgagca taattcttca gaaaaatagc gatgttccat gttgtcaggg
     6901 catgcatgat gcacgttatg aggtgacggt gctaggcagt attccctcaa agtttcatag
     6961 tcagtatcat attcatcatt gcattcctgc aagagagaat tgagacgcaa tccacacgct
     7021 gcggcaacct tccggcgttc gtggtctatt tgctcttgga cgttgcaaac gtaagtgttg
     7081 gatcccggtc ggcatctact ctattccttt gccctcggac gagtgctggg gcgtcggttt
     7141 ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg
     7201 atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc
     7261 gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca
     7321 atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg
     7381 aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg
     7441 acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg
     7501 ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg
     7561 gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg
     7621 gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa
     7681 tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc
     7741 tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg
     7801 ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa
     7861 taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc
     7921 gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc
     7981 cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc
     8041 gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac
     8101 gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct ctccaaatga aatgaacttc
     8161 cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
     8221 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     8281 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     8341 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     8401 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     8461 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     8521 tggagtagac gagagtgtcg tgctccacca tgttgacgga tctctaggac gcgtcgacgc
     8581 gtcgacgcgt cgacgcgtcg acgcgtccta gaagctaatt cactggccgt cgttttacaa
     8641 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     8701 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     8761 agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8821 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8881 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     8941 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     9001 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     9061 gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
     9121 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
     9181 ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt tattaagttg
     9241 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     9301 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     9361 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     9421 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     9481 tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc ctcgcagaga
     9541 tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg tcgatcttga
     9601 gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc tgagtggcgc
     9661 tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct caccgaatgg
     9721 tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc tgatcgaccc
     9781 cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc
     9841 cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga
     9901 gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga
     9961 gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt
    10021 gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg
    10081 cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag
    10141 ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag
    10201 agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca
    10261 ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt gcgccgagga
    10321 tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc acgagcaata
    10381 aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc tgttcgggct
    10441 ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc tcctgtttga
    10501 agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca
    10561 accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca
    10621 tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg
    10681 ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct ctgtttatcg
    10741 gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc
    10801 agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc cagccggaac
    10861 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc
    10921 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt
    10981 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg
    11041 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta
    11101 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc
    11161 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag
    11221 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg
    11281 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag
    11341 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc
    11401 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt
    11461 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg
    11521 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc
    11581 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg
    11641 ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc
    11701 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga
    11761 cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg
    11821 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg
    11881 ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct
    11941 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc
    12001 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg
    12061 ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac
    12121 cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt
    12181 ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc
    12241 ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc
    12301 atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa tattttattt
    12361 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
    12421 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
    12481 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
    12541 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
    12601 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
    12661 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
    12721 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
    12781 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
    12841 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
    12901 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
    12961 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
    13021 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
    13081 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
    13141 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
    13201 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
    13261 ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg tgatgctgcc
    13321 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
    13381 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
    13441 acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt tcacttacac
    13501 cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg gcgttggatg
    13561 ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc acttaaaaaa
    13621 ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg
    13681 gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg gctgttttac
    13741 gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactatggcg
    13801 acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg gatgacggat
    13861 ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag caagcgatat
    13921 acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg gctgggacgg
    13981 aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg gcattatctg
    14041 aacataaaac actatcaata agttggagtc attacccaat tatgatagaa tttacaagct
    14101 ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg ctttgcccat
    14161 tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc cttacatacg
    14221 gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat attcaaggca
    14281 atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat atggcgcttc
    14341 atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa tcttacctat
    14401 cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc acccgcgacc
    14461 actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc cgtgaatgcc
    14521 ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact gcccggcacc
    14581 tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg cgtcgcgctc
    14641 gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac tgccagcgct
    14701 gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg
    14761 cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg
    14821 tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc
    14881 aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga
    14941 ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact
    15001 ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg
    15061 tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta
    15121 aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg
    15181 agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc
    15241 cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc
    15301 gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc
    15361 cgccagccca gcggcgaggg caaccagccc ggtgagcgtc ggaaaggcgc
//
