LOCUS       sequence-246969-       16177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16177)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX090
FEATURES             Location/Qualifiers
     source          1..16177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6291..6315)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6344..6433
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6467..6719
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7148..7400)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7868..8893)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7868..8893)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8903..9247)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9389..9406)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9389..9405)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9398..9420)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9545..9564)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9755..9776
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10023..10047
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11643..12791)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13090..13884)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14076..14843
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15536..16167
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg atttgtactg aggatgatca
     4621 attattgggt tggaaagatt ttccaaaggg gcttagagtc ctacttcttg atgaagactg
     4681 caattctgct gctgaaatga gatcaaggct tgaaaaattg gattacatag tctactcatt
     4741 ctgcaatgag aacgaagctt tgtctgcaat cacaaacaaa tctgaggact ttcatgttgc
     4801 cattgtggag gtaactgctg gcaacagtga tggagttctc cgatttcttg agagtgccaa
     4861 agatctgcca acaataatga catcaaatgt tcattccctt agcacaatga tgaagtgtat
     4921 atcgcttggt gcagtcgagt ttcttcagaa accattatca gatgataaac tcaaaaatat
     4981 ctggcaacat gtagttcgca aggcattcaa tggtggagaa aaagatgtgc ccaaatcgca
     5041 taaaccagta aaagaatctc ttgtttccat gccacagcta caacaagtaa agagtgcagc
     5101 cgatgacaaa agttcaaatg aaacggaacc ttatacctca gtaccagaca acaataatga
     5161 acagtcatct ggctgcgata aataccctgc tccgtcaacc ccacaattga aacaaggagt
     5221 gaggtcagtg gatgatggtg attgccaaga tcataccatc ttttcaaccg aacaagatag
     5281 tggggagcat gatggggaaa ctaaatccgt cgaaactact tttaacaata caattgctga
     5341 gagaactgtc caaacaagtc ctcctgagca acaaggacag acaattgtga aagaggagaa
     5401 tggttcatct cctcatcaaa aggtggaggc tgatattgct acctcttcac tgagtaacaa
     5461 ctgccctgac aatagcagta gtcattctgc tgaacctagt aaagcatctg ctccccatag
     5521 ttcaagtggg actaaaacca ataagaagaa gatgaaggta gattggtcac ctgaactaca
     5581 caagaagttt gttcaagcag tagagcaact tggtattgat caagccattc cttctcgaat
     5641 actagagctg atgaaagtgg agggaataac gagacataac gtagctagcc atctccagaa
     5701 atacagaatg catcggaggc aaattttacc aaaggaagtg gaaaggagat ggcctcatcc
     5761 acaaccaaga gattcagtac aaaggagcta ttatcctcat catagaccta tcatgaccgt
     5821 cccaccatat cattctaatc atgtccccgc agctggtcaa ttgtatcctg cttgggtacc
     5881 accggcaagt tatccgactg gtttacaagt gtggggttca ccttactatc cagcatggca
     5941 gcctgcagat tcttggcact ggaatcctca tccagggctg catgccgata catggggctc
     6001 ccctgtcatg ccaccatcat taggatcata tccgccgtat cctcagaatg gtggagttta
     6061 ccggcctaac ggaatgcaga atagatatag catgctagag aagtcatttg atcttcaccc
     6121 agctgaggag gttattgata aagtagtaaa ggaggcaata accaaaccat ggttgcctct
     6181 tcctttgggc ctaaaacgtc cttccacgga gagtgttctc cacgagcttt caagacaagg
     6241 gatctcaacc gtcccaccac aaatcaacgg ctctcgtcgg agccccgggg acccagcttt
     6301 cttgtacaaa gtggttgata acagcgggtt aattaacatc ttttacccat acgatgttcc
     6361 tgactatgcg ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt
     6421 tccagattac gctgctcagt gcagcgctta gagctcgaat ttccccgatc gttcaaacat
     6481 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     6541 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     6601 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     6661 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     6721 ggaattagct tcatcaacgc aagacatgcg cacgaccgtc tgacaggaga ggaatttccg
     6781 acgagcacag aaaggacttg ctcttggacg taggcctatt tctcaggcac atgtatcaag
     6841 tgttcggacg tgggttttcg atggtgtatc agccgccgcc aactgggaga tgaggaggct
     6901 ttcttggggg gcagtcagca gttcatttca caagacagag gaacttgtaa ggagatgcac
     6961 tgatttatct tggcgcaaac cagcaggacg aattagtggg aatagcccgc gaatatctaa
     7021 gttatgcctg tcggcatgag cagaaacttc caattcgaaa cagtttggag aggttgtttt
     7081 tgggcatacc ttttgttagt cagcctctcg attgctcatc gtcattacac agtaccgaag
     7141 tttgatcgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
     7201 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
     7261 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
     7321 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
     7381 tgccaaatgt ttgaacgatc tgcttcgacg cactccttct ttactccacc atctcgtcct
     7441 tattgaaaac gtgggtagca ccaaaacgaa tcaagtcgct ggaactgaag ttaccaatca
     7501 cgctggatga tttgccagtt ggattaatct tgcctttccc cgcatgaata atattgatga
     7561 atgcatgcgt gaggggtatt tcgattttgg caatagctgc aattgccgcg acatcctcca
     7621 acgagcataa ttcttcagaa aaatagcgat gttccatgtt gtcagggcat gcatgatgca
     7681 cgttatgagg tgacggtgct aggcagtatt ccctcaaagt ttcatagtca gtatcatatt
     7741 catcattgca ttcctgcaag agagaattga gacgcaatcc acacgctgcg gcaaccttcc
     7801 ggcgttcgtg gtctatttgc tcttggacgt tgcaaacgta agtgttggat cccggtcggc
     7861 atctactcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     7921 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     7981 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     8041 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     8101 acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     8161 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     8221 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     8281 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     8341 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     8401 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     8461 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     8521 ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt
     8581 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
     8641 cgctgaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     8701 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     8761 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     8821 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     8881 caggcttttt catatcgggg tcgtcctctc caaatgaaat gaacttcctt atatagagga
     8941 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
     9001 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
     9061 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
     9121 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
     9181 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
     9241 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
     9301 agtgtcgtgc tccaccatgt tgacggatct ctaggacgcg tcgacgcgtc gacgcgtcga
     9361 cgcgtcgacg cgtcctagaa gctaattcac tggccgtcgt tttacaacgt cgtgactggg
     9421 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     9481 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     9541 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9601 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9661 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9721 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9781 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa
     9841 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
     9901 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
     9961 aaaccacccc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
    10021 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
    10081 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
    10141 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
    10201 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
    10261 gacagagcgt tgctgcctgt gatcaaatat catctccctc gcagagatcc gaattatcag
    10321 ccttcttatt catttctcgc ttaaccgtga caggctgtcg atcttgagaa ctatgccgac
    10381 ataataggaa atcgctggat aaagccgctg aggaagctga gtggcgctat ttctttagaa
    10441 gtgaacgttg acgatatcaa ctcccctatc cattgctcac cgaatggtac aggtcgggga
    10501 cccgaagttc cgactgtcgg cctgatgcat ccccggctga tcgaccccag atctggggct
    10561 gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc ggaaccaaag
    10621 gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa cattggttcc
    10681 tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca gaagtcctcc
    10741 ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc acttgcgggt
    10801 cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga cgccgacagg
    10861 atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc cgcaaatagc
    10921 ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga tgaacacgac
    10981 catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc ggtagaccga
    11041 aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga agatgcgcat
    11101 ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac ccgccggcaa
    11161 cgcccgcagc agcataccgg cgacccctcg gcctcgctgt tcgggctcca cgaaaacgcc
    11221 ggacagatgc gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga ccgacagccc
    11281 aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc gttcagcgaa
    11341 cgcctccatg ggctttttct cctcgtgctc gtaaacggac ccgaacatct ctggagcttt
    11401 cttcagggcc gacaatcgga tctcgcggaa atcctgcacg tcggccgctc caagccgtcg
    11461 aatctgagcc ttaatcacaa ttgtcaattt taatcctctg tttatcggca gttcgtagag
    11521 cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt
    11581 tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg
    11641 ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg
    11701 cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa
    11761 tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag
    11821 ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg
    11881 aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg
    11941 caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat
    12001 tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg
    12061 cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc
    12121 tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc
    12181 tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc
    12241 acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg
    12301 ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc
    12361 ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt
    12421 ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt
    12481 tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct
    12541 gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca
    12601 gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag
    12661 ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg
    12721 gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac
    12781 gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct
    12841 tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg
    12901 aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc
    12961 tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc ctgagagcca
    13021 aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac
    13081 atctaggtac taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg
    13141 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct
    13201 gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag
    13261 atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc
    13321 gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg
    13381 cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt
    13441 attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc
    13501 cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc
    13561 agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag
    13621 cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc
    13681 ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg
    13741 gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat ataccttagc
    13801 aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc
    13861 cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag
    13921 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
    13981 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
    14041 gtatcgacgg agccgatttt gaaaccacaa ttatgggtga tgctgccaac ttactgattt
    14101 agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc
    14161 tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat
    14221 ctctgctctc actgccgtaa aacatggcaa ctgcagttca cttacaccgc ttctcaaccc
    14281 ggtacgcacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg ggcaacagcc
    14341 cgcattatgg gcgttggcct caacacgatt ttacgtcact taaaaaactc aggccgcagt
    14401 cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac
    14461 agtggggcta tgtcggggct aaatcgcgcc agcgctggct gttttacgcg tatgacagtc
    14521 tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc
    14581 ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc tggccgctgt
    14641 atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg cagcgaattg
    14701 agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag tcgctgtcgt
    14761 tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac ataaaacact
    14821 atcaataagt tggagtcatt acccaattat gatagaattt acaagctata aggttattgt
    14881 cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat
    14941 tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg atatcttcta
    15001 tataaaagat atattatctt atcagtattg tcaatatatt caaggcaatc tgcctcctca
    15061 tcctcttcat cctcttcgtc ttggtagctt tttaaatatg gcgcttcata gagtaattct
    15121 gtaaaggtcc aattctcgtt ttcatacctc ggtataatct tacctatcac ctcaaatggt
    15181 tcgctgggtt tatcgcaccc ccgaacacga gcacggcacc cgcgaccact atgccaagaa
    15241 tgcccaaggt aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg acggccgaag
    15301 tgaagggcag gccgccaccc aggccgccgc cctcactgcc cggcacctgg tcgctgaatg
    15361 tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg ctgatcgccc
    15421 atcccgttac tgccccgatc ccggcaatgg caaggactgc cagcgctgcc atttttgggg
    15481 tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg
    15541 gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg
    15601 gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca
    15661 ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg
    15721 gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt
    15781 gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg
    15841 gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt
    15901 tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca
    15961 tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg
    16021 tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca
    16081 cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg
    16141 gcgagggcaa ccagcccggt gagcgtcgga aaggcgc
//
