LOCUS       sequence-246971-       16186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16186)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX092
FEATURES             Location/Qualifiers
     source          1..16186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6300..6324)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6353..6442
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6476..6728
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7157..7409)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7877..8902)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7877..8902)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8912..9256)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9398..9415)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9398..9414)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9407..9429)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9554..9573)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9764..9785
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10032..10056
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11652..12800)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13099..13893)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14085..14852
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15545..16176
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg tctttagtta gatctgtaag
     4621 aactattgga aatgggaaac tctactttcc aaatgatcac aacgacaact ctggcttgtc
     4681 aacatccatg ttcaccaaaa atgcacgtgg aattatgtat gcaaccgagt ctagcagcac
     4741 agacagttat gatcccaagt atttgctcga ttctccttca ccttcagaag aacttctaaa
     4801 cacatcactt tcagaagtct ccgggaatca atttcaccag aggcattcgt cttctttcca
     4861 gccatcaaga ggctacgata catgtcagct tagttatgat tgtgaagaca ttgtcagtca
     4921 aaggccagat tcattacaat acaatgacgg aagggtaact ctgaagctac aagaacttga
     4981 gagcgtactt tttgacgata acgaagttga tggggatgac gtatttgctc gtgggaagtg
     5041 tatggacata gatgaagaat ggtttagcca aatcggaact gtgttgcttc atgactctcc
     5101 taaggagtct acgtcggcag attctaatat cagtagcagt agtatctaca aggaaatatc
     5161 tgtaactgct ccacaaactc caaagcagat gctttttagt tgtgctgctg ctattcaaga
     5221 tggaaacatt gaacaagcat cgtcgatgat taatgagctg aggcaaatgg tctctatcca
     5281 gggtgaacct cttgaaagaa ctgcagctta catggttgaa gcgcttgcgg cccgagtggc
     5341 cacatctggc aaaggtcttt ataaagctct aaaatgtaag gaagcgctct tttctgagag
     5401 actctctgca atgcaggtcc tctttgaagt atgtccatac ttcaggtttg gttttatggc
     5461 ggcgaatggt gcaatcttag aggcttttaa agatgagaaa agagttcaca tcatagattt
     5521 tgacattaac caaggaagtc aatattacac tttattgcag acgttggaac gtatgcctgg
     5581 taagccgcca catttgaggt taaccggggt agacgaccct gtatctgttc aacgtgctat
     5641 tggaggcctt aatgtcattg gactgaggct tgaagagctg gcaaaagatt ttaagattcc
     5701 ttttgaattc caagcggtgg cttcaaacac tgcattagtc accccggcaa tgttaaactg
     5761 tcatcctggc gaagctctta tcgtgaactt tgcattccag cttcatcaca tgcctgatga
     5821 aagtgtatcc acggtcaacc agagagacca gcttctgcgg atggtgaaga gcttaaatcc
     5881 gaagctggta acagttgttg aacaagacat gaacactaac accaccccat ttctacaaag
     5941 ggttgcagaa gtctacaact attattctgc tgtattcgag tctctcgatg caactctttc
     6001 aagagatagc caagagaggg ttaatgtaga acggcaatgt cttgcgcgtg atattgtaaa
     6061 cattgttgcc tgtgagggag aggaaagaat agagcgttat gaagttgcag gaaaatggag
     6121 agctaggatg atgatggctg gtttcactcc atccccgatc agtcgaaacg tctactactc
     6181 gatccagaat cttatcaagc aatatagtga aaggtacaaa gcaaaggagg aggcaggtgc
     6241 actccatttt gggtgggaag acaagagctt gattgttgct tcagcttgga aacccgggga
     6301 cccagctttc ttgtacaaag tggttgataa cagcgggtta attaacatct tttacccata
     6361 cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag gatcctatcc
     6421 atatgacgtt ccagattacg ctgctcagtg cagcgcttag agctcgaatt tccccgatcg
     6481 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     6541 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     6601 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     6661 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     6721 actagatcgg gaattagctt catcaacgca agacatgcgc acgaccgtct gacaggagag
     6781 gaatttccga cgagcacaga aaggacttgc tcttggacgt aggcctattt ctcaggcaca
     6841 tgtatcaagt gttcggacgt gggttttcga tggtgtatca gccgccgcca actgggagat
     6901 gaggaggctt tcttgggggg cagtcagcag ttcatttcac aagacagagg aacttgtaag
     6961 gagatgcact gatttatctt ggcgcaaacc agcaggacga attagtggga atagcccgcg
     7021 aatatctaag ttatgcctgt cggcatgagc agaaacttcc aattcgaaac agtttggaga
     7081 ggttgttttt gggcatacct tttgttagtc agcctctcga ttgctcatcg tcattacaca
     7141 gtaccgaagt ttgatcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     7201 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     7261 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     7321 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     7381 aaactttatt gccaaatgtt tgaacgatct gcttcgacgc actccttctt tactccacca
     7441 tctcgtcctt attgaaaacg tgggtagcac caaaacgaat caagtcgctg gaactgaagt
     7501 taccaatcac gctggatgat ttgccagttg gattaatctt gcctttcccc gcatgaataa
     7561 tattgatgaa tgcatgcgtg aggggtattt cgattttggc aatagctgca attgccgcga
     7621 catcctccaa cgagcataat tcttcagaaa aatagcgatg ttccatgttg tcagggcatg
     7681 catgatgcac gttatgaggt gacggtgcta ggcagtattc cctcaaagtt tcatagtcag
     7741 tatcatattc atcattgcat tcctgcaaga gagaattgag acgcaatcca cacgctgcgg
     7801 caaccttccg gcgttcgtgg tctatttgct cttggacgtt gcaaacgtaa gtgttggatc
     7861 ccggtcggca tctactctat tcctttgccc tcggacgagt gctggggcgt cggtttccac
     7921 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
     7981 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
     8041 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
     8101 ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt
     8161 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
     8221 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
     8281 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
     8341 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
     8401 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
     8461 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
     8521 taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca
     8581 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
     8641 tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
     8701 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
     8761 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
     8821 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
     8881 cggtgagttc aggctttttc atatcggggt cgtcctctcc aaatgaaatg aacttcctta
     8941 tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
     9001 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
     9061 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
     9121 agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct
     9181 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
     9241 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
     9301 gtagacgaga gtgtcgtgct ccaccatgtt gacggatctc taggacgcgt cgacgcgtcg
     9361 acgcgtcgac gcgtcgacgc gtcctagaag ctaattcact ggccgtcgtt ttacaacgtc
     9421 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     9481 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     9541 tgaatggcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     9601 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     9661 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     9721 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     9781 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     9841 atttcggaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     9901 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     9961 tgaaaagaaa aaccacccca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
    10021 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
    10081 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
    10141 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
    10201 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
    10261 tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc atctccctcg cagagatccg
    10321 aattatcagc cttcttattc atttctcgct taaccgtgac aggctgtcga tcttgagaac
    10381 tatgccgaca taataggaaa tcgctggata aagccgctga ggaagctgag tggcgctatt
    10441 tctttagaag tgaacgttga cgatatcaac tcccctatcc attgctcacc gaatggtaca
    10501 ggtcggggac ccgaagttcc gactgtcggc ctgatgcatc cccggctgat cgaccccaga
    10561 tctggggctg agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg
    10621 gaaccaaagg aagtaggtta aacccgctcc gatcaggccg agccacgcca ggccgagaac
    10681 attggttcct gtaggcatcg ggattggcgg atcaaacact aaagctactg gaacgagcag
    10741 aagtcctccg gccgccagtt gccaggcggt aaaggtgagc agaggcacgg gaggttgcca
    10801 cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac
    10861 gccgacagga tctagcgctg cgtttggtgt caacaccaac agcgccacgc ccgcagttcc
    10921 gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta cctagcagag cggcagagat
    10981 gaacacgacc atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg
    11041 gtagaccgaa ataaacaaca agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa
    11101 gatgcgcatc caccagattc ccgttggaat ctgtcggacg atcatcacga gcaataaacc
    11161 cgccggcaac gcccgcagca gcataccggc gacccctcgg cctcgctgtt cgggctccac
    11221 gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac
    11281 cgacagccca atgatctcgc cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg
    11341 ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg taaacggacc cgaacatctc
    11401 tggagctttc ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc
    11461 aagccgtcga atctgagcct taatcacaat tgtcaatttt aatcctctgt ttatcggcag
    11521 ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg tcgagcagtg
    11581 cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc cggaactgac
    11641 cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc
    11701 aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg
    11761 cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc
    11821 cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc
    11881 tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc
    11941 aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc
    12001 gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg
    12061 cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc
    12121 tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac
    12181 tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag
    12241 gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg
    12301 attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc
    12361 atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc
    12421 ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg
    12481 ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc
    12541 gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg
    12601 ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg
    12661 accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg
    12721 atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc
    12781 cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca cgccggggca
    12841 atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata atccacctta
    12901 tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt ggtattccga
    12961 atcttgccct gcacgaatac cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc
    13021 tgagagccaa aacacttgat gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg
    13081 ttgcgccaca tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
    13141 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
    13201 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
    13261 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
    13321 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
    13381 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
    13441 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
    13501 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
    13561 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
    13621 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
    13681 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
    13741 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
    13801 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
    13861 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
    13921 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    13981 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    14041 tataacatag tatcgacgga gccgattttg aaaccacaat tatgggtgat gctgccaact
    14101 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
    14161 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat
    14221 cagcgctatc tctgctctca ctgccgtaaa acatggcaac tgcagttcac ttacaccgct
    14281 tctcaacccg gtacgcacca gaaaatcatt gatatggcca tgaatggcgt tggatgccgg
    14341 gcaacagccc gcattatggg cgttggcctc aacacgattt tacgtcactt aaaaaactca
    14401 ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa
    14461 tggacgaaca gtggggctat gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt
    14521 atgacagtct ccggaagacg gttgttgcgc acgtattcgg tgaacgcact atggcgacgc
    14581 tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt gatatggatg acggatggct
    14641 ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt aatcagcaag cgatatacgc
    14701 agcgaattga gcggcataac ctgaatctga ggcagcacct ggcacggctg ggacggaagt
    14761 cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt catcgggcat tatctgaaca
    14821 taaaacacta tcaataagtt ggagtcatta cccaattatg atagaattta caagctataa
    14881 ggttattgtc ctgggtttca agcattagtc catgcaagtt tttatgcttt gcccattcta
    14941 tagatatatt gataagcgcg ctgcctatgc cttgccccct gaaatcctta catacggcga
    15001 tatcttctat ataaaagata tattatctta tcagtattgt caatatattc aaggcaatct
    15061 gcctcctcat cctcttcatc ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag
    15121 agtaattctg taaaggtcca attctcgttt tcatacctcg gtataatctt acctatcacc
    15181 tcaaatggtt cgctgggttt atcgcacccc cgaacacgag cacggcaccc gcgaccacta
    15241 tgccaagaat gcccaaggta aaaattgccg gccccgccat gaagtccgtg aatgccccga
    15301 cggccgaagt gaagggcagg ccgccaccca ggccgccgcc ctcactgccc ggcacctggt
    15361 cgctgaatgt cgatgccagc acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc
    15421 tgatcgccca tcccgttact gccccgatcc cggcaatggc aaggactgcc agcgctgcca
    15481 tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg
    15541 cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac
    15601 gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt
    15661 taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt
    15721 ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc
    15781 ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa
    15841 taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat
    15901 caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc
    15961 tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc
    16021 cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg
    16081 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc
    16141 agcccagcgg cgagggcaac cagcccggtg agcgtcggaa aggcgc
//
