LOCUS       sequence-246972-       14760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14760)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 14760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX093
FEATURES             Location/Qualifiers
     source          1..14760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    2911..2935
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4874..4898)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4927..5016
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5050..5302
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(5731..5983)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6451..7476)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6451..7476)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7486..7830)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(7972..7989)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7972..7988)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7981..8003)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8128..8147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8338..8359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8606..8630
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10226..11374)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11673..12467)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12659..13426
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14119..14750
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag aacacggggg acaagtttgt acaaaaaagc aggctccacc
     2941 actagtatgt actatcaagg aacctcggat aatattcatc aagccgctga tggtaatatt
     3001 cagacacttt atctgatgaa ccctaactat attcatcaag ggtacagtat tgacacacaa
     3061 caacaacaac aacaacaaca ccatcagcaa caaattatac gccacgcgcc gctgcaggca
     3121 cagcagcact ttatcggcgt ccctcttccg gcagttagtt tgcacgatca gaatcatgcc
     3181 cagcttatcc accgtctgtg gaaccaaagt ggtggtggtg gtggaaatgg aagtcatcac
     3241 caatcccaga ttataccgtc atccacggtg gtttccgcca cctcctgtgg cggcagaacc
     3301 acggacttgg cctctcagtt ggggttccag aggccaatga acatgtcgcc gacacaacaa
     3361 caacaaggtg gtctatcgct aagcctttct cctcaacaac aaattagttt caataacatt
     3421 tcagctcaga atcaagttgg atcaaatatt aatagaggat tagatggaat ttccagcatg
     3481 gttttaggtt ctaagtacct gaaagctgca caagagcttc tggatgaagt tgttaatgtt
     3541 ggaaaatgta tcaaaggaag tgaattggag aagggggaat taatgaataa agaatcaata
     3601 ttgcctttgg ctggtgatgg gaacactagt aatagtggag gtgaaagtag cagcaagcag
     3661 aaaaatggag ttgctgaact tacaactgct caaagacaag aatttcaaat gaagaaagcc
     3721 aagcttgtta ccatgcttga agaggtggag caaagataca gacagtacca tcaccaaatg
     3781 caaataattg tatcatcatt ggagcaagta gcaggaattg gatccgcaaa atcatacaca
     3841 caactggctt tgcatgcaat ttcgaagcaa ttccgatgct taaaagatgc tattgttgga
     3901 caaataaagg ccatgagcaa gagtttgggt gaagaagatc aaagcttagg agggaaaatt
     3961 gaagggtcac gatttaaatt tgtagaccat catcttaggc aacaacgtgc acttcaacag
     4021 ataggaatga tgcaaccaaa tgcttggaga ccccaaagag gtttacctga aagagctgtc
     4081 tctgtccttc gtgcttggct tttcgagcat tttcttcatc cttatcccaa ggattccgac
     4141 aaaatcattc ttgcgaagca aaccgggcta acaaggagcc aggtctccaa ctggttcata
     4201 aatgctcgag ttcgactatg gaagccaatg gtggaagaaa tgtacttgga agaggtgaag
     4261 aataatcaag aacaaaatag ctcatcagaa gaaaacaaaa acaaaaatga atccaacaaa
     4321 gagaaaatgg tctcaaaatc aagtgctcca caagaaaatg gtgctccaac agaaattgca
     4381 acctcaacta ttacaactcc tcctactgca tgtggctcat catttcaagc agtacaagct
     4441 cacaactttt cctttcttgg ttcattaaac atggaaaatt gtactactgc taaaaagcca
     4501 agaaatcatg acatgcataa taattctcca agtagtattc tttcatcggt agaaatggac
     4561 gaaaccaaag ctagagatca tcaatcaaac aaagggttca atcctttaat ggatgcagca
     4621 gcagtttatg caatgggaga atttggaagg tttaatccgc atgaacaaat ggcagcaaca
     4681 aattttcatg gaaataatgg tgtctctctt actttagggc ttcctcctaa ttctgaaaac
     4741 ctgagccaac agagttacca aaatactatt gagttcaata gaatgagcta tgaaaacatt
     4801 gattttcaga ataagcggtt cccttctcaa ctattaccag atttcgttgc aggtaatctt
     4861 ggaacacccg gggacccagc tttcttgtac aaagtggttg ataacagcgg gttaattaac
     4921 atcttttacc catacgatgt tcctgactat gcgggctatc cctatgacgt cccggactat
     4981 gcaggatcct atccatatga cgttccagat tacgctgctc agtgcagcgc ttagagctcg
     5041 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     5101 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     5161 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     5221 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     5281 gtgtcatcta tgttactaga tcgggaatta gcttcatcaa cgcaagacat gcgcacgacc
     5341 gtctgacagg agaggaattt ccgacgagca cagaaaggac ttgctcttgg acgtaggcct
     5401 atttctcagg cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt atcagccgcc
     5461 gccaactggg agatgaggag gctttcttgg ggggcagtca gcagttcatt tcacaagaca
     5521 gaggaacttg taaggagatg cactgattta tcttggcgca aaccagcagg acgaattagt
     5581 gggaatagcc cgcgaatatc taagttatgc ctgtcggcat gagcagaaac ttccaattcg
     5641 aaacagtttg gagaggttgt ttttgggcat accttttgtt agtcagcctc tcgattgctc
     5701 atcgtcatta cacagtaccg aagtttgatc gatctagtaa catagatgac accgcgcgcg
     5761 ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt
     5821 gcgggactct aatcataaaa acccatctca taaataacgt catgcattac atgttaatta
     5881 ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag
     5941 gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttcg acgcactcct
     6001 tctttactcc accatctcgt ccttattgaa aacgtgggta gcaccaaaac gaatcaagtc
     6061 gctggaactg aagttaccaa tcacgctgga tgatttgcca gttggattaa tcttgccttt
     6121 ccccgcatga ataatattga tgaatgcatg cgtgaggggt atttcgattt tggcaatagc
     6181 tgcaattgcc gcgacatcct ccaacgagca taattcttca gaaaaatagc gatgttccat
     6241 gttgtcaggg catgcatgat gcacgttatg aggtgacggt gctaggcagt attccctcaa
     6301 agtttcatag tcagtatcat attcatcatt gcattcctgc aagagagaat tgagacgcaa
     6361 tccacacgct gcggcaacct tccggcgttc gtggtctatt tgctcttgga cgttgcaaac
     6421 gtaagtgttg gatcccggtc ggcatctact ctattccttt gccctcggac gagtgctggg
     6481 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     6541 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     6601 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     6661 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     6721 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     6781 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     6841 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     6901 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     6961 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     7021 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     7081 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     7141 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     7201 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     7261 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     7321 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     7381 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     7441 ctcgacagac gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct ctccaaatga
     7501 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
     7561 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
     7621 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
     7681 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
     7741 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
     7801 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
     7861 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttgacgga tctctaggac
     7921 gcgtcgacgc gtcgacgcgt cgacgcgtcg acgcgtccta gaagctaatt cactggccgt
     7981 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8041 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8101 acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8161 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8221 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     8281 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8341 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     8401 aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac
     8461 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     8521 gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt
     8581 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
     8641 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
     8701 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     8761 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     8821 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc
     8881 ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg
     8941 tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc
     9001 tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct
     9061 caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc
     9121 tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt
     9181 cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac
     9241 gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct
     9301 actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc
     9361 acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc
     9421 aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc
     9481 acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc
     9541 agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc
     9601 ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt
     9661 gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc
     9721 acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc
     9781 tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc
     9841 tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg
     9901 gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg
     9961 gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc
    10021 acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct
    10081 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
    10141 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
    10201 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
    10261 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
    10321 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
    10381 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
    10441 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
    10501 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
    10561 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
    10621 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
    10681 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
    10741 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
    10801 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
    10861 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
    10921 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
    10981 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
    11041 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
    11101 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
    11161 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
    11221 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
    11281 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
    11341 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
    11401 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
    11461 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
    11521 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
    11581 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
    11641 tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa
    11701 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    11761 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    11821 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    11881 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    11941 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    12001 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    12061 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    12121 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    12181 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    12241 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
    12301 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    12361 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    12421 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    12481 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    12541 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    12601 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg
    12661 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
    12721 ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
    12781 gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt
    12841 tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg
    12901 gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc
    12961 acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat
    13021 cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg
    13081 gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg
    13141 cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg
    13201 gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag
    13261 caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg
    13321 gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg
    13381 gcattatctg aacataaaac actatcaata agttggagtc attacccaat tatgatagaa
    13441 tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg
    13501 ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc
    13561 cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat
    13621 attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat
    13681 atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa
    13741 tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc
    13801 acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc
    13861 cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact
    13921 gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg
    13981 cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac
    14041 tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg
    14101 ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg
    14161 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
    14221 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
    14281 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
    14341 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
    14401 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
    14461 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
    14521 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
    14581 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
    14641 gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc
    14701 catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc ggaaaggcgc
//
