LOCUS       sequence-246973-       11854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A self-assembling split-GFP based Golden Gate vector to determine 
            protein targeting to mitochondria in plant cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB
  TITLE     Targeting specificity of nuclear-encoded organelle proteins with a 
            self-assembling split-fluorescent protein toolkit.
  JOURNAL   J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 
            10.1242/jcs.230839.
  PUBMED    31085714
REFERENCE   2  (bases 1 to 11854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-06-3"; volume: "132(11). pii: jcs.230839. doi"; 
            pages: " 10.1242/jcs.230839"
COMMENT     Sequence Label: pMiNGo11-7
FEATURES             Location/Qualifiers
     source          1..11854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1030..1375
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1769..2410
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     terminator      2441..3148
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        4165..4510
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(4640..4945)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4742..4761)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5265..5948)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     5863..5882
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5949..6051)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6082..6507
                     /codon_start=1
                     /product="seven tandem copies of the 11th beta-strand of 
                     superfolder GFP"
                     /label=GFP11x7
                     /note="each GFP11 associates with GFP(1-10), consisting of 
                     the first 10 beta-strands, to reconstitute fluorescent GFP 
                     (Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDH
                     MVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSG
                     GRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGIT"
     misc_feature    7212..7236
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7516..8145
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8574..9647
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9713..9907
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9969..10073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10074..10868
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11039..11627
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11528..11547
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11763..11787
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgatgt atcacctctc gcaagaattc aagcttggag gaattccaat cccacaaaaa
       61 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
      121 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
      181 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
      241 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
      301 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
      361 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
      421 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
      481 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
      541 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
      601 gcaagtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
      661 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
      721 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
      781 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
      841 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tcaagactgg
      901 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
      961 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca
     1021 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     1081 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     1141 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     1201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac
     1261 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     1321 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
     1381 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
     1441 aattacattt acaattatcg ataccatgct tcgagtagca ggtagaaggc tttcttcttc
     1501 agccgctaga tcttcatcta ccttctttac aagaagctct ttcaccgtta ccgatgattc
     1561 gtctccggca agatctcctt ctccgtcact cacctcttcg tttctcgatc aaatcagagg
     1621 tttctcatct aattcggttt ctcccgcaca tcagttgggt ttagtctcag atcttccagc
     1681 cacagtggct gctattaaga atcccagttc aaaaattgta tatgatgact ccaaccatga
     1741 gcgttatcca cctggagatc ctagcgcaat gtccaaagga gaagaactgt ttaccggtgt
     1801 tgtgccaatt ttggttgaac tcgatggtga tgtcaacgga cataagttct cagtgagagg
     1861 cgaaggagaa ggtgacgcca ccattggaaa attgactctt aaattcatct gtactactgg
     1921 taaacttcct gtaccatggc cgactctcgt aacaacgctt acgtacggag ttcagtgctt
     1981 ttcgagatac ccagaccata tgaaaagaca tgactttttt aagtcggcta tgcctgaagg
     2041 ttacgtgcaa gaaagaacaa tttcgttcaa agatgatgga aaatataaaa ctagagcagt
     2101 tgttaaattt gaaggagata ctttggttaa ccgcattgaa ctgaaaggaa cagattttaa
     2161 agaagatggt aatattcttg gacacaaact cgaatacaat tttaatagtc ataacgtata
     2221 catcactgct gataagcaaa agaacggaat taaagcgaat ttcacagtac gccataatgt
     2281 agaagatggc agtgttcaac ttgccgacca ttaccaacaa aacaccccta ttggagacgg
     2341 tccggtactt cttcctgata atcactacct ctcaacacaa acagtcctga gcaaagatcc
     2401 aaatgaaaaa ggaacaggtg gcggcggaag ttagcttgtc ctgctttaat gagatatgcg
     2461 agaagcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg taaaaaacct
     2521 gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga atatatcacc
     2581 cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt gtaccctact
     2641 acttatatgt acaatattaa aatgaaaaca atatattgtg ctgaataggt ttatagcgac
     2701 atctatgata gagcgccaca ataacaaaca attgcgtttt attattacaa atccaatttt
     2761 aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt acataaatct tattcaaatt
     2821 tcaaaagtgc cccaggggct agtatctacg acacaccgag cggcgaacta ataacgctca
     2881 ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga agttgagtat
     2941 tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc acggcggccg
     3001 ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg tgggccccaa
     3061 atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat
     3121 tttgcgacaa catgtcgagg ctcagcagga ccgctactag aattcgagct cggaggaatt
     3181 ccaatcccac aaaaatctga gcttaacagc acagttgctc ctctcagagc agaatcgggt
     3241 attcaacacc ctcatatcaa ctactacgtt gtgtataacg gtccacatgc cggtatatac
     3301 gatgactggg gttgtacaaa ggcggcaaca aacggcgttc ccggagttgc acacaagaaa
     3361 tttgccacta ttacagaggc aagagcagca gctgacgcgt acacaacaag tcagcaaaca
     3421 gacaggttga acttcatccc caaaggagaa gctcaactca agcccaagag ctttgctaag
     3481 gccctaacaa gcccaccaaa gcaaaaagcc cactggctca cgctaggaac caaaaggccc
     3541 agcagtgatc cagccccaaa agagatctcc tttgccccgg agattacaat ggacgatttc
     3601 ctctatcttt acgatctagg aaggaagttc gaaggtgaag gtgacgacac tatgttcacc
     3661 actgataatg agaaggttag cctcttcaat ttcagaaaga atgctgaccc acagatggtt
     3721 agagaggcct acgcagcaag tctcatcaag acgatctacc cgagtaacaa tctccaggag
     3781 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taattgcatc
     3841 aagaacacag agaaagacat atttctcaag atcagaagta ctattccagt atggacgatt
     3901 caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
     3961 cctactgaat ctaaggccat gcatggagtc taagattcaa atcgaggatc taacagaact
     4021 cgccgtcaag actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa
     4081 aatcttcgtc aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
     4141 agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
     4201 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
     4261 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
     4321 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt
     4381 tccaaccacg tctacaaagc aagtggattg atgtgacatc tccactgacg taagggatga
     4441 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     4501 ggagaggaca cgctcgagta taagagctca tttttacaac aattaccaac aacaacaaac
     4561 aacaaacaac attacaatta catttacaat tatcgataca atgtgagacc gactggctgt
     4621 gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt
     4681 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggaaacc ggcacactgg
     4741 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
     4801 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
     4861 caggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
     4921 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg
     4981 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
     5041 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
     5101 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
     5161 gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat
     5221 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
     5281 acgcggcgtt tacgcccccc ctgccactca tcgcagtact gttgtaattc attaagcatt
     5341 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     5401 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     5461 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa
     5521 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     5581 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     5641 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     5701 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     5761 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     5821 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     5881 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     5941 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     6001 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aaggtctcat
     6061 tcgatgggcg gcaaattcat gcgtgaccac atggtccttc atgagtatgt aaatgctgct
     6121 gggattacag gtggctctgg aggtagagat catatggttc tccacgaata cgttaacgcc
     6181 gcaggcatca ctggcggtag tggaggacgc gaccatatgg tactacatga atatgtcaat
     6241 gcagccggaa taaccggagg gtccggaggc cgggatcaca tggtgctgca tgagtatgtg
     6301 aacgcggcgg gtataactgg tgggtcgggc ggacgagatc atatggtgct tcacgaatac
     6361 gtaaacgcag ctggcattac tggcggatca ggtggcaggg atcacatggt actccatgag
     6421 tacgtgaacg ctgctggaat cacaggcggt agcggcggtc gggaccatat ggtcctgcac
     6481 gaatatgtca atgctgccgg tatcacctaa gcttagagct ttcgttcgta tcatcggttt
     6541 cgacaacgtt cgtcaagttc aatgcatcag tttcattgcg cacacaccag aatcctactg
     6601 agtttgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa
     6661 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
     6721 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct
     6781 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt
     6841 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacacttg
     6901 tagttgtacc attatgctta ttcactaggc aacaaatata ttttcagacc tagaaaagct
     6961 gcaaatgtta ctgaatacaa gtatgtcctc ttgtgtttta gacatttatg aactttcctt
     7021 tatgtaattt tccagaatcc ttgtcagatt ctaatcattg ctttgtaatt atagttatac
     7081 tcatggattt gtagttgagt atgaaaatat tttttaatgc attttatgac ttgccaattg
     7141 attgacaacg ctttacgagg atgcacatgt gaccgaggga cacgaagtga tccgtttaaa
     7201 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     7261 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgcc
     7321 agccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     7381 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     7441 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
     7501 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     7561 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     7621 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     7681 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     7741 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     7801 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     7861 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     7921 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     7981 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     8041 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     8101 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     8161 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     8221 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     8281 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     8341 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     8401 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     8461 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
     8521 gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
     8581 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     8641 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     8701 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     8761 gtgcgtcgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     8821 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     8881 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     8941 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     9001 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     9061 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     9121 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     9181 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     9241 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     9301 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     9361 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     9421 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     9481 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     9541 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     9601 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     9661 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aagcttcttt cctgtggata
     9721 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     9781 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     9841 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     9901 gtgcataact gtctggccag cgcacagccg aacagctgca aaaagcgccc acatcaaggc
     9961 tccgagtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    10021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatggcta
    10081 aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata
    10141 cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt
    10201 taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca
    10261 tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg
    10321 atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg
    10381 aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc
    10441 actccatcga catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat
    10501 tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg gaagaggaca
    10561 ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg
    10621 aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag
    10681 taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct
    10741 tctgcgtccg gtcgctcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg
    10801 acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat
    10861 tgttttagct gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    10921 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    10981 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11041 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11101 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11161 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    11221 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11281 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11341 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    11401 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    11461 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    11521 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    11581 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    11641 cggccttttt acggttcctg ctcggatctg ttggaccgga cagtagtcat ggttgatggg
    11701 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
    11761 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
    11821 cggacgtttt taatgtactg gggttgaaca ctct
//
