LOCUS       sequence-246974-       11476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A self-assembling split-GFP based Golden Gate vector to determine 
            protein targeting to mitochondria in plant cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11476)
  AUTHORS   Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB
  TITLE     Targeting specificity of nuclear-encoded organelle proteins with a 
            self-assembling split-fluorescent protein toolkit.
  JOURNAL   J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 
            10.1242/jcs.230839.
  PUBMED    31085714
REFERENCE   2  (bases 1 to 11476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-06-3"; volume: "132(11). pii: jcs.230839. doi"; 
            pages: " 10.1242/jcs.230839"
COMMENT     Sequence Label: pMiNGo11-1
FEATURES             Location/Qualifiers
     source          1..11476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1030..1375
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1769..2410
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     terminator      2441..3148
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        4165..4510
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(4640..4945)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4742..4761)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5265..5948)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     5863..5882
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5949..6051)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6082..6129
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     misc_feature    6834..6858
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7138..7767
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8196..9269
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9335..9529
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9591..9695
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9696..10490
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10661..11249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11150..11169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11385..11409
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgatgt atcacctctc gcaagaattc aagcttggag gaattccaat cccacaaaaa
       61 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
      121 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
      181 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
      241 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
      301 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
      361 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
      421 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
      481 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
      541 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
      601 gcaagtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
      661 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
      721 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
      781 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
      841 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tcaagactgg
      901 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
      961 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca
     1021 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     1081 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     1141 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     1201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac
     1261 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     1321 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
     1381 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
     1441 aattacattt acaattatcg ataccatgct tcgagtagca ggtagaaggc tttcttcttc
     1501 agccgctaga tcttcatcta ccttctttac aagaagctct ttcaccgtta ccgatgattc
     1561 gtctccggca agatctcctt ctccgtcact cacctcttcg tttctcgatc aaatcagagg
     1621 tttctcatct aattcggttt ctcccgcaca tcagttgggt ttagtctcag atcttccagc
     1681 cacagtggct gctattaaga atcccagttc aaaaattgta tatgatgact ccaaccatga
     1741 gcgttatcca cctggagatc ctagcgcaat gtccaaagga gaagaactgt ttaccggtgt
     1801 tgtgccaatt ttggttgaac tcgatggtga tgtcaacgga cataagttct cagtgagagg
     1861 cgaaggagaa ggtgacgcca ccattggaaa attgactctt aaattcatct gtactactgg
     1921 taaacttcct gtaccatggc cgactctcgt aacaacgctt acgtacggag ttcagtgctt
     1981 ttcgagatac ccagaccata tgaaaagaca tgactttttt aagtcggcta tgcctgaagg
     2041 ttacgtgcaa gaaagaacaa tttcgttcaa agatgatgga aaatataaaa ctagagcagt
     2101 tgttaaattt gaaggagata ctttggttaa ccgcattgaa ctgaaaggaa cagattttaa
     2161 agaagatggt aatattcttg gacacaaact cgaatacaat tttaatagtc ataacgtata
     2221 catcactgct gataagcaaa agaacggaat taaagcgaat ttcacagtac gccataatgt
     2281 agaagatggc agtgttcaac ttgccgacca ttaccaacaa aacaccccta ttggagacgg
     2341 tccggtactt cttcctgata atcactacct ctcaacacaa acagtcctga gcaaagatcc
     2401 aaatgaaaaa ggaacaggtg gcggcggaag ttagcttgtc ctgctttaat gagatatgcg
     2461 agaagcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg taaaaaacct
     2521 gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga atatatcacc
     2581 cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt gtaccctact
     2641 acttatatgt acaatattaa aatgaaaaca atatattgtg ctgaataggt ttatagcgac
     2701 atctatgata gagcgccaca ataacaaaca attgcgtttt attattacaa atccaatttt
     2761 aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt acataaatct tattcaaatt
     2821 tcaaaagtgc cccaggggct agtatctacg acacaccgag cggcgaacta ataacgctca
     2881 ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga agttgagtat
     2941 tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc acggcggccg
     3001 ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg tgggccccaa
     3061 atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat
     3121 tttgcgacaa catgtcgagg ctcagcagga ccgctactag aattcgagct cggaggaatt
     3181 ccaatcccac aaaaatctga gcttaacagc acagttgctc ctctcagagc agaatcgggt
     3241 attcaacacc ctcatatcaa ctactacgtt gtgtataacg gtccacatgc cggtatatac
     3301 gatgactggg gttgtacaaa ggcggcaaca aacggcgttc ccggagttgc acacaagaaa
     3361 tttgccacta ttacagaggc aagagcagca gctgacgcgt acacaacaag tcagcaaaca
     3421 gacaggttga acttcatccc caaaggagaa gctcaactca agcccaagag ctttgctaag
     3481 gccctaacaa gcccaccaaa gcaaaaagcc cactggctca cgctaggaac caaaaggccc
     3541 agcagtgatc cagccccaaa agagatctcc tttgccccgg agattacaat ggacgatttc
     3601 ctctatcttt acgatctagg aaggaagttc gaaggtgaag gtgacgacac tatgttcacc
     3661 actgataatg agaaggttag cctcttcaat ttcagaaaga atgctgaccc acagatggtt
     3721 agagaggcct acgcagcaag tctcatcaag acgatctacc cgagtaacaa tctccaggag
     3781 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taattgcatc
     3841 aagaacacag agaaagacat atttctcaag atcagaagta ctattccagt atggacgatt
     3901 caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
     3961 cctactgaat ctaaggccat gcatggagtc taagattcaa atcgaggatc taacagaact
     4021 cgccgtcaag actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa
     4081 aatcttcgtc aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
     4141 agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
     4201 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
     4261 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
     4321 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt
     4381 tccaaccacg tctacaaagc aagtggattg atgtgacatc tccactgacg taagggatga
     4441 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     4501 ggagaggaca cgctcgagta taagagctca tttttacaac aattaccaac aacaacaaac
     4561 aacaaacaac attacaatta catttacaat tatcgataca atgtgagacc gactggctgt
     4621 gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt
     4681 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggaaacc ggcacactgg
     4741 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
     4801 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
     4861 caggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
     4921 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg
     4981 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
     5041 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
     5101 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
     5161 gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat
     5221 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
     5281 acgcggcgtt tacgcccccc ctgccactca tcgcagtact gttgtaattc attaagcatt
     5341 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     5401 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     5461 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa
     5521 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     5581 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     5641 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     5701 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     5761 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     5821 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     5881 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     5941 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     6001 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aaggtctcat
     6061 tcgatgggcg gcaaattcat gcgtgaccac atggtccttc atgagtatgt aaatgctgct
     6121 gggattacat aagcttagag ctttcgttcg tatcatcggt ttcgacaacg ttcgtcaagt
     6181 tcaatgcatc agtttcattg cgcacacacc agaatcctac tgagtttgag tattatggca
     6241 ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt
     6301 cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg
     6361 gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg
     6421 aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt
     6481 ggcctctaat gaccgaagtt aatatgagga gtaaaacact tgtagttgta ccattatgct
     6541 tattcactag gcaacaaata tattttcaga cctagaaaag ctgcaaatgt tactgaatac
     6601 aagtatgtcc tcttgtgttt tagacattta tgaactttcc tttatgtaat tttccagaat
     6661 ccttgtcaga ttctaatcat tgctttataa ttatagttat actcatggat ttgtagttga
     6721 gtatgaaaat attttttaat gcattttatg acttgccaat tgattgacaa cgctttacga
     6781 ggatgcacat gtgaccgagg gacacgaagt gatccgttta aactatcagt gtttgacagg
     6841 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     6901 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg ccagccgtgc ggctgcatga
     6961 aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga
     7021 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca
     7081 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg
     7141 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat
     7201 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag
     7261 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc
     7321 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc
     7381 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg
     7441 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg
     7501 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg
     7561 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg
     7621 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc
     7681 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc
     7741 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca
     7801 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca
     7861 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca
     7921 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat
     7981 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg
     8041 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc
     8101 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc
     8161 aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc
     8221 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
     8281 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
     8341 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgtcg tcgattagga
     8401 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
     8461 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
     8521 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
     8581 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
     8641 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
     8701 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
     8761 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
     8821 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
     8881 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
     8941 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
     9001 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
     9061 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
     9121 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
     9181 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
     9241 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
     9301 tagcagggga aaaaggtcga aaaagcttct ttcctgtgga tagcacgtac attgggaacc
     9361 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
     9421 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
     9481 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
     9541 agcgcacagc cgaacagctg caaaaagcgc ccacatcaag gctccgagtg cgcggaaccc
     9601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9661 gataaatgct tcaataatat tgaaaaagga agagtatggc taaaatgaga atatcaccgg
     9721 aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg
     9781 ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc
     9841 ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag
     9901 gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc
     9961 tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg
    10021 aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg
    10081 attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata
    10141 acgatctggc cgatgtggat tgcgaaaact gggaagagga cactccattt aaagatccgc
    10201 gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg
    10261 gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc
    10321 ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgctca
    10381 gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc
    10441 ctgattggga gaaaataaaa tattatattt tactggatga attgttttag ctgtcagacc
    10501 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    10561 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10621 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    10681 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10741 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10801 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    10861 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    10921 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    10981 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    11041 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    11101 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    11161 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    11221 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    11281 tgctcggatc tgttggaccg gacagtagtc atggttgatg ggctgcctgt atcgagtggt
    11341 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
    11401 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac
    11461 tggggttgaa cactct
//
