LOCUS       sequence-246976-       16432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16432)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX094
FEATURES             Location/Qualifiers
     source          1..16432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6546..6570)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6599..6688
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6722..6974
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7403..7655)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8123..9148)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8123..9148)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9158..9502)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9644..9661)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9644..9660)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9653..9675)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9800..9819)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10010..10031
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10278..10302
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11898..13046)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13345..14139)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14331..15098
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15791..16422
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg gcaacttatt ttcctaatcc
     4621 aaacaatcaa agagaagctg agacatttca atattttagg caatctttgc ctggttctta
     4681 ttcagaagct tcaaatgctc aagaaaacat gatggtgttt gtgaactatt cttcttctgg
     4741 gacatattca gatatgttga ccagtacttc gcaacagcaa aacagctgca tcgatatccc
     4801 accagttgga gactccgatt ccaacagcac atcacaacag gaaatattat ctaatcttgg
     4861 aggatcgcgg atgggaattc atgatttttc tgcttggaga gatagcagga atgagatgct
     4921 atacctcagt cttggctcta atataccatc tggaattgga atttcacatg tccaatctca
     4981 gaatcctagc caaggtctta cgtcattctt gaatcctgct tcatctattc caggtgaagt
     5041 tagtggtggc aatgtgtcct tcggagatgg tgataattcc caaccaaaac aacaaagaaa
     5101 tgcagattat ttgcctccag attatcctgg aagcaactca gatgctataa atggagggta
     5161 taattctcca tatggtacgt cgagtattgg aagaaatgtt cccaactcaa agtatttgaa
     5221 agcagctcag tatttgcttg atgaggttgt taatgtcaga aaggctatca aggagcataa
     5281 ttctaagaaa gagttgacaa cggagtctag agagtttgat ggcgactcga aaaataaatc
     5341 gtcagatcct cctgcaaatg ggagtacaaa tcctcaagat tcgaaaaatc accaaagtga
     5401 actttcacaa accgagaagc aagaggtgca gaacaaactg accaagcttc tgtcgatgct
     5461 ggatgagatt gatagaaagt acagacaata ttatcatcag atgcaaatag tggtctcatc
     5521 atttgatgtg gtagctggag atggagcagc taagccctac acagctcttg ctctccagac
     5581 aatttcccgt cactttcgtt gcttgcgtga tgcagtctgc gatcagattc gagtatcacg
     5641 aagatgtctt ggagagcaag atgcttcaga aaacagcaaa gcggttggaa tatcacgctt
     5701 gcgctatgtg gatcagcata ttagacagca gagagccctg caacagcttg gtatgatgca
     5761 acaacatgcc tggaggcctc agaggggtct gcctgaaagc tctgtttcaa ttttgcgcgc
     5821 ttggcttttc gagcattttc ttcatcccta tccgaaagat tctgacaaaa ttatgctagc
     5881 aagacaaact ggtttaacga gaagtcaggt atcaaattgg tttataaatg cacgggtacg
     5941 tctttggaag cctatggttg aggaaatgta caaagaagaa gctggtgata ttgaaatgga
     6001 ctcaaattct ccatcagaat ttgcctccag acttgcaaca aaagactcaa aagtcgagga
     6061 aacagaagaa ttgaaacata atgaaacctc agaatatgag cagtacaata gtgaccaaat
     6121 cttggagtca aaatccaacc atgttactga tgtagaaatg gaaggaggaa ataatgcaga
     6181 aactcaaatt caatctcgaa tggaaaatca aactcaaaga gacgaaccac ggcctgctat
     6241 ggataattgc accctttttc aggacacatt tgttcaaggc aatgatagat tcttagattt
     6301 tggtagattg ggaggtggaa atgtacttcc taatggagtt tcacttacac tggggttgca
     6361 gcaaggtgaa ggaagcagct taccgatgtc tatcgagtct cacggtagtt atgtaccgtt
     6421 aagggtagat gagatctata gcgcagcacc tactaccatg gtctctgaga cagcagaatt
     6481 caactgtttg gattctggga acaggcagca tcctttttgg ctcctaccat ctgctacacc
     6541 cggggaccca gctttcttgt acaaagtggt tgataacagc gggttaatta acatctttta
     6601 cccatacgat gttcctgact atgcgggcta tccctatgac gtcccggact atgcaggatc
     6661 ctatccatat gacgttccag attacgctgc tcagtgcagc gcttagagct cgaatttccc
     6721 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     6781 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     6841 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     6901 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     6961 tatgttacta gatcgggaat tagcttcatc aacgcaagac atgcgcacga ccgtctgaca
     7021 ggagaggaat ttccgacgag cacagaaagg acttgctctt ggacgtaggc ctatttctca
     7081 ggcacatgta tcaagtgttc ggacgtgggt tttcgatggt gtatcagccg ccgccaactg
     7141 ggagatgagg aggctttctt ggggggcagt cagcagttca tttcacaaga cagaggaact
     7201 tgtaaggaga tgcactgatt tatcttggcg caaaccagca ggacgaatta gtgggaatag
     7261 cccgcgaata tctaagttat gcctgtcggc atgagcagaa acttccaatt cgaaacagtt
     7321 tggagaggtt gtttttgggc ataccttttg ttagtcagcc tctcgattgc tcatcgtcat
     7381 tacacagtac cgaagtttga tcgatctagt aacatagatg acaccgcgcg cgataattta
     7441 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
     7501 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
     7561 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
     7621 cttaagaaac tttattgcca aatgtttgaa cgatctgctt cgacgcactc cttctttact
     7681 ccaccatctc gtccttattg aaaacgtggg tagcaccaaa acgaatcaag tcgctggaac
     7741 tgaagttacc aatcacgctg gatgatttgc cagttggatt aatcttgcct ttccccgcat
     7801 gaataatatt gatgaatgca tgcgtgaggg gtatttcgat tttggcaata gctgcaattg
     7861 ccgcgacatc ctccaacgag cataattctt cagaaaaata gcgatgttcc atgttgtcag
     7921 ggcatgcatg atgcacgtta tgaggtgacg gtgctaggca gtattccctc aaagtttcat
     7981 agtcagtatc atattcatca ttgcattcct gcaagagaga attgagacgc aatccacacg
     8041 ctgcggcaac cttccggcgt tcgtggtcta tttgctcttg gacgttgcaa acgtaagtgt
     8101 tggatcccgg tcggcatcta ctctattcct ttgccctcgg acgagtgctg gggcgtcggt
     8161 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg
     8221 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
     8281 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
     8341 caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct
     8401 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
     8461 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc
     8521 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt
     8581 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
     8641 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga
     8701 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
     8761 tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag
     8821 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
     8881 aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg
     8941 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta
     9001 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
     9061 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
     9121 acgtcgcggt gagttcaggc tttttcatat cggggtcgtc ctctccaaat gaaatgaact
     9181 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
     9241 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
     9301 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
     9361 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
     9421 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
     9481 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
     9541 cttggagtag acgagagtgt cgtgctccac catgttgacg gatctctagg acgcgtcgac
     9601 gcgtcgacgc gtcgacgcgt cgacgcgtcc tagaagctaa ttcactggcc gtcgttttac
     9661 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     9721 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     9781 gcagcctgaa tggcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9841 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     9901 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     9961 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    10021 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
    10081 ttgccgattt cggaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
    10141 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
    10201 cactggtgaa aagaaaaacc accccagtac attaaaaacg tccgcaatgt gttattaagt
    10261 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
    10321 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
    10381 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
    10441 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
    10501 gttgattgta acgatgacag agcgttgctg cctgtgatca aatatcatct ccctcgcaga
    10561 gatccgaatt atcagccttc ttattcattt ctcgcttaac cgtgacaggc tgtcgatctt
    10621 gagaactatg ccgacataat aggaaatcgc tggataaagc cgctgaggaa gctgagtggc
    10681 gctatttctt tagaagtgaa cgttgacgat atcaactccc ctatccattg ctcaccgaat
    10741 ggtacaggtc ggggacccga agttccgact gtcggcctga tgcatccccg gctgatcgac
    10801 cccagatctg gggctgagaa agcccagtaa ggaaacaact gtaggttcga gtcgcgagat
    10861 cccccggaac caaaggaagt aggttaaacc cgctccgatc aggccgagcc acgccaggcc
    10921 gagaacattg gttcctgtag gcatcgggat tggcggatca aacactaaag ctactggaac
    10981 gagcagaagt cctccggccg ccagttgcca ggcggtaaag gtgagcagag gcacgggagg
    11041 ttgccacttg cgggtcagca cggttccgaa cgccatggaa accgcccccg ccaggcccgc
    11101 tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc
    11161 agttccgcaa atagccccca ggaccgccat caatcgtatc gggctaccta gcagagcggc
    11221 agagatgaac acgaccatca gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg
    11281 caggcggtag accgaaataa acaacaagct ccagaatagc gaaatattaa gtgcgccgag
    11341 gatgaagatg cgcatccacc agattcccgt tggaatctgt cggacgatca tcacgagcaa
    11401 taaacccgcc ggcaacgccc gcagcagcat accggcgacc cctcggcctc gctgttcggg
    11461 ctccacgaaa acgccggaca gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt
    11521 gaagaccgac agcccaatga tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg
    11581 caaccgttca gcgaacgcct ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa
    11641 catctctgga gctttcttca gggccgacaa tcggatctcg cggaaatcct gcacgtcggc
    11701 cgctccaagc cgtcgaatct gagccttaat cacaattgtc aattttaatc ctctgtttat
    11761 cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga
    11821 gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga
    11881 actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt
    11941 tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc
    12001 ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac
    12061 ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg
    12121 tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg
    12181 tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc
    12241 agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc
    12301 tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc
    12361 aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc
    12421 gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg
    12481 gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg
    12541 cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc
    12601 gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc
    12661 tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg
    12721 tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc
    12781 gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat
    12841 ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct
    12901 tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg
    12961 cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat
    13021 gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc
    13081 ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc
    13141 accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta
    13201 ttccgaatct tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc
    13261 tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg
    13321 gcatcgttgc gccacatcta ggtactaaaa caattcatcc agtaaaatat aatattttat
    13381 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc
    13441 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct
    13501 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct
    13561 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa
    13621 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac
    13681 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt
    13741 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag
    13801 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc
    13861 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg
    13921 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt
    13981 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag
    14041 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat
    14101 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt
    14161 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
    14221 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
    14281 ttggcgtata acatagtatc gacggagccg attttgaaac cacaattatg ggtgatgctg
    14341 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
    14401 atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc
    14461 ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca gttcacttac
    14521 accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa tggcgttgga
    14581 tgccgggcaa cagcccgcat tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa
    14641 aactcaggcc gcagtcggta acctcgcgca tacagccggg cagtgacgtc atcgtctgcg
    14701 cggaaatgga cgaacagtgg ggctatgtcg gggctaaatc gcgccagcgc tggctgtttt
    14761 acgcgtatga cagtctccgg aagacggttg ttgcgcacgt attcggtgaa cgcactatgg
    14821 cgacgctggg gcgtcttatg agcctgctgt caccctttga cgtggtgata tggatgacgg
    14881 atggctggcc gctgtatgaa tcccgcctga agggaaagct gcacgtaatc agcaagcgat
    14941 atacgcagcg aattgagcgg cataacctga atctgaggca gcacctggca cggctgggac
    15001 ggaagtcgct gtcgttctca aaatcggtgg agctgcatga caaagtcatc gggcattatc
    15061 tgaacataaa acactatcaa taagttggag tcattaccca attatgatag aatttacaag
    15121 ctataaggtt attgtcctgg gtttcaagca ttagtccatg caagttttta tgctttgccc
    15181 attctataga tatattgata agcgcgctgc ctatgccttg ccccctgaaa tccttacata
    15241 cggcgatatc ttctatataa aagatatatt atcttatcag tattgtcaat atattcaagg
    15301 caatctgcct cctcatcctc ttcatcctct tcgtcttggt agctttttaa atatggcgct
    15361 tcatagagta attctgtaaa ggtccaattc tcgttttcat acctcggtat aatcttacct
    15421 atcacctcaa atggttcgct gggtttatcg cacccccgaa cacgagcacg gcacccgcga
    15481 ccactatgcc aagaatgccc aaggtaaaaa ttgccggccc cgccatgaag tccgtgaatg
    15541 ccccgacggc cgaagtgaag ggcaggccgc cacccaggcc gccgccctca ctgcccggca
    15601 cctggtcgct gaatgtcgat gccagcacct gcggcacgtc aatgcttccg ggcgtcgcgc
    15661 tcgggctgat cgcccatccc gttactgccc cgatcccggc aatggcaagg actgccagcg
    15721 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
    15781 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
    15841 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
    15901 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
    15961 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
    16021 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
    16081 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
    16141 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
    16201 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
    16261 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
    16321 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
    16381 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gc
//
