LOCUS       sequence-24698-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Kim DS, Matsuda T, Cepko CL
  TITLE     A core paired-type and POU homeodomain-containing transcription 
            factor program drives retinal bipolar cell gene expression.
  JOURNAL   J Neurosci. 2008 Jul 30. 28(31):7748-64.
  PUBMED    18667607
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Jul 30. 28(31):7748-64."
COMMENT     Sequence Label: Chx10 (164bp crit)+SV40p-tdTomato-IRES-AP
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        195..391
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      242..377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      430..439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(569..587)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     gap             778..790
                     /estimated_length=13
ORIGIN
        1 gcagggngac attcgagaga gcactggctg gggctgcttg cccgctaatc ccagctgcca
       61 ttaaaatatt aaagataaat ctaatcgtct ctttatccaa aataagcgac ttttgtgtgg
      121 ggagaaaacg tctaacccct taggaggaga attagttcta atgcatcaaa tggaattgct
      181 cgagatctgc gatctgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
      241 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatcgct gactaatttt
      301 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
      361 aggctttttt ggaggcctag gcttttgcaa aaagcttggt accgcgggcc cgggatccac
      421 cgggatcccg ccaccatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc
      481 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
      541 ggccgcccct acnagggcac ccanaccgcc aagctgaagg tgaccaaggg cggccccctg
      601 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccnaggc gtacgtgaag
      661 caccccgccg acatccccga ttacaanaag ctgtccttcc ccgaggctnn ngtgggacgc
      721 gtgatgactt ngaggacggc ggtctggtga cgtgacccag gatctcngng accgcccnnn
      781 nnnnnnnnnn aaaannnnnn ncnntncccc nnanccnnnn naaaaaanng gntgg
//
