LOCUS       sequence-246982-       13492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Eukaryotic expression of the fusion protein used for drug diruption 
            the p53-HDM2 interaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Scarabelli S, Tan KT, Griss R, Hovius R, D'Alessandro PL, Vorherr T,
            Johnsson K
  TITLE     Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins.
  JOURNAL   ACS Sens. 2017 Aug 25;2(8):1191-1197. doi: 
            10.1021/acssensors.7b00331. Epub 2017 Aug 2.
  PUBMED    28766337
REFERENCE   2  (bases 1 to 13492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.7b00331"; journalName: "ACS Sens"; date: 
            "2017-08-25- 25"; volume: "2"; issue: "8"; pages: "1191-1197"
COMMENT     Sequence Label: pEBTet-p52-HDM2_sensor
FEATURES             Location/Qualifiers
     source          1..13492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     193..212
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     240..259
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        240..258
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             269..889
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(342..361)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    931..1052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        1107..1486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1487..1690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1641..1661
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1692..1710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1713..1731
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1741..1765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1865..1888
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1895..1909
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             1967..2680
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     CDS             join(2804..2806,2807..2809,2810..3322)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(2849..2870)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3110..3129)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3457..3478
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3899..3928
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(3969..3986)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3975..4086
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4627..6416
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(6718..8643)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGXGXGGGXGXGGGXGGXGGXGXGGGXGXGGGXGGXGGGGGG
                     GGXGGXGXGGGXGXGGGXGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGXGGAGGAGXGGGXGGXGAG
                     GGXGGXGXGGXGGXGXGGXGGXGXGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(9078..9096)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9164..9268
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9269..9337,9338..10129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9487..9506)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10300..10888
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10789..10808
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(11077..11097)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11095..11392
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             11469..12068
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11469..11488)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11965..11985
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    12159..12293
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12431..12450)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        12727..12988
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          join(13105..13492,1..185)
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg
       61 tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa
      121 aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc
      181 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgt
      241 aatacgactc actatagggc gaattgatat gtctagatta gataaaagta aagtgattaa
      301 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
      361 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
      421 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
      481 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
      541 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
      601 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
      661 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
      721 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
      781 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
      841 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg cgtacagcgg
      901 atcccgggaa ttcagatctt attaaagcag aacttgttta ttgcagctta taatggttac
      961 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     1021 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggtcgaatcc atggcgcaga
     1081 actggtaggt atggaagatc cgcgttgaca ttgattattg actagttatt aatagtaatc
     1141 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1201 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1261 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1321 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1381 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1441 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     1501 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     1561 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     1621 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     1681 aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg
     1741 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     1801 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttaccat
     1861 ggcctggagc cacccgcagt tcgaaaaggg tgcagatgac gacgacaagg taccgagcca
     1921 ggaaaccttt agcgatctgt ggaaactgct gccggaaaat agtactatgt ctaaaggtga
     1981 agaattattc actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca
     2041 caaattttct gtctccggtg aaggtgaagg tgatgctacg tacggtaaat tgaccttaaa
     2101 attactctgt actactggta aattgccagt tccatggcca accttagtca ctactttagg
     2161 ttatggtgtt caatgttttg ctagataccc agatcatatg aaacaacatg actttttcaa
     2221 gtctgccatg ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa
     2281 ctacaagacc agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt
     2341 aaaaggtatt gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta
     2401 taactctcac aatgtttaca tcactgctga caaacaaaag aatggtatca aagctaactt
     2461 caaaattaga cacaacattg aagatggtgg tgttcaatta gctgaccatt atcaacaaaa
     2521 tactccaatt ggtgatggtc cagtcttgtt accagacaac cattacttat cctatcaatc
     2581 tgccttattc aaagatccaa acgaaaagag agaccacatg gtcttgttag aatttttgac
     2641 tgctgctggt attaccgagg gtatgaatga attgtacaaa gaattccctc ctcctccgcc
     2701 accgccacca ccgccgccgc cgccaccacc aggaggatct ggcggatctc ctcctccgcc
     2761 tcctccacca ccaccgccac cgcctccacc acctggcggc cgcatggtga gcaagggcga
     2821 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2881 caggttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2941 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3001 ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3061 gtccgccatg cccgaaggct acgtccagga gcgtaccatc ttcttcaagg acgacggcaa
     3121 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3181 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3241 catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggcccactt
     3301 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3361 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3421 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3481 cgccgccact agtatgtgca ataccaacat gtctgtacct actgatggtg ctgtaaccac
     3541 ctcacagatt ccagcttcgg aacaagagac cctggttaga ccaaagccat tgcttttgaa
     3601 gttattaaag tctgttggtg cacaaaaaga cacttatact atgaaagagg ttctttttta
     3661 tcttggccag tatattatga ctaaacgatt atatgatgag aagcaacaac atattgtata
     3721 ttgttcaaat gatcttctag gagatttgtt tggcgtgcca agcttctctg tgaaagagca
     3781 caggaagata tataccatga tctacaggaa cttggtagta gtcaatcagc aggaatcatc
     3841 ggactcaggt acatctgtga gtgagaaccg agctccgggc ttctcctcaa tttccgctca
     3901 tcaccaccat catcaccatc accaccacta actcgagtct agagggcccg tttaaacccg
     3961 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4021 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4081 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4141 caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc cccggacgaa
     4201 ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt aatcccttca
     4261 gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg gggggaccaa
     4321 acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc acatttgcca
     4381 acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt
     4441 gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc
     4501 caggggccca ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc
     4561 taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta
     4621 accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta
     4681 attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag
     4741 ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta tttacatggg
     4801 gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa
     4861 ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct tcgtttagct
     4921 aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt
     4981 ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc attgtgctat
     5041 gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag gaatgaaaca
     5101 ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa agactggatg
     5161 tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa tatgatactg
     5221 gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt gttacactct
     5281 atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact ggttgtctct
     5341 aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca aagacaagtg
     5401 gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac acgccgccct
     5461 gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc ccgctaacca
     5521 ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat acctgcataa
     5581 gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac
     5641 acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga
     5701 gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat
     5761 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagtatat
     5821 gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagyatat
     5881 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagtatat
     5941 gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat
     6001 gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata gcatatgcta
     6061 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gcataggcta
     6121 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     6181 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     6241 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
     6301 tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt gctatccttt
     6361 gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt ttcctgctgg
     6421 ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca tgtctgattg
     6481 ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc gcggagctga
     6541 gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa aatggtgaca
     6601 agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga tttattcttt
     6661 agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca agttacatca
     6721 ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat ctccgtcatc
     6781 accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat ctactccatc
     6841 gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg tcataacaag
     6901 gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa agaccatgaa
     6961 ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag gggccattcc
     7021 aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg cagttcctcg
     7081 ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg cgacccaagt
     7141 tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta aaccttctgc
     7201 aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa accaccctcc
     7261 ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc cttctcctgg
     7321 gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac catctgggcc
     7381 accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa atggccttct
     7441 acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac tgggactcct
     7501 gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac ttccccccct
     7561 ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc ggcctccact
     7621 acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc ctgctcctgc
     7681 ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctccts ctcctscccc
     7741 tcctscccct cctsctccts cccctcctsc ccctcctsct cctscccctc ctscccctcc
     7801 tcctgctcct scccctccts cccctcctcc tsctcctgcc cctcctgccc ctcctsctcc
     7861 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     7921 tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgcccctc ctgcccctcc
     7981 tcctgctcct gcccctcctg ctcctgcccc tcctgcccct cctgcccctc ctgctcctgc
     8041 ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     8101 tscccctcct cctsctccts cccctcctcc tsctcctscc cctcctsccc ctcctccccc
     8161 tcctcctcct cctscccctc ctscccctcc tcctsctcct scccctcctc ctsctcctsc
     8221 ccctcctscc cctcctsccc ctcctcctsc tcctscccct cctcctsctc ctscccctcc
     8281 tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc
     8341 tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca
     8401 gccaatgcaa cttggacgtt tttggggtct ccggacacca tctctatgtc ttggccctga
     8461 tcctgagccg cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc
     8521 tcgtccatgg ttatcacccc ctcttctttg aggtccactg ccgccggagc cttctggtcc
     8581 agatgtgtct cccttctctc ctaggccatt tccaggtcct gtacctggcc cctcgtcaga
     8641 catgattcac actaaaagag atcaatagac atctttatta gacgacgctc agtgaataca
     8701 gggagtgcag actcctgccc cctccaacag cccccccacc ctcatcccct tcatggtcgc
     8761 tgtcagacag atccaggtct gaaaattccc catcctccga accatcctcg tcctcatcac
     8821 caattactcg cagcccggaa aactcccgct gaacatcctc aagatttgcg tcctgagcct
     8881 caagccaggc ctcaaattcc tcgtccccct ttttgctgga cggtagggat ggggattctc
     8941 gggacccctc ctcttcctct tcaaggtcac cagacagaga tgctactggg gcaacggaag
     9001 aaaagctggg tgcggcctgt gaggatcagc ttatcgatga taagctgtca aacatgagaa
     9061 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     9121 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9181 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9241 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9301 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9361 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9421 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9481 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     9541 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     9601 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     9661 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     9721 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9781 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     9841 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9901 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9961 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    10021 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    10081 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    10141 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10201 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10261 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10321 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10381 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    10441 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    10501 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    10561 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    10621 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    10681 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    10741 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10801 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10861 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10921 ggccttttgc tggccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat
    10981 ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc
    11041 catccagcct cgcgtcgaac tagatgatcc ggctgtggaa tgtgtgtcag ttagggtgtg
    11101 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    11161 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    11221 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
    11281 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    11341 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggagggtgac
    11401 cgccacgacc ggtgccgcca ccatcccctg acccacgccc ctgacccctc acaaggagac
    11461 gaccttccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
    11521 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
    11581 accccgaccg ccacatcgaa cgcgtcaccg agctgcaaga actcttcctc acgcgcgtcg
    11641 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
    11701 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
    11761 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
    11821 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
    11881 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
    11941 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
    12001 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
    12061 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgacccggtc
    12121 cgacgcggcc cgacgggtcc gaggggggtc gacctcgaaa cttgtttatt gcagcttata
    12181 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    12241 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatccg
    12301 aaccccttcc tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
    12361 tgttgccatt gctcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgtatggt
    12421 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
    12481 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
    12541 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
    12601 tacgggccag atatacgcgt atctgagggg actagggtgt gtttaggcga aaagcggggc
    12661 ttcggttgta cgcggttagg agtcccctca ggatatagta gtttcgcttt tgcataggga
    12721 gggggaaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
    12781 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
    12841 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
    12901 tgaattccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgccat
    12961 ttgaccattc accacattgg tgtgcacctc caagctggcc ggggatcctc tagggcctct
    13021 gagctattcc agaagtagtg aagaggcttt tttggaggcc taggcttttg caaaaagctc
    13081 cggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc
    13141 gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg
    13201 ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta
    13261 ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta
    13321 aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt
    13381 aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca
    13441 gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt tc
//
