LOCUS       sequence-246987-        2787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression vector for Escherichia coli. BglBrick 
            compatible..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Jervis AJ, Carbonell P, Taylor S, Sung R, Dunstan MS, Robinson CJ, 
            Breitling R, Takano E, Scrutton NS
  TITLE     SelProm: A Queryable and Predictive Expression Vector Selection Tool
            for Escherichia coli.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00399.
  PUBMED    30870592
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00399."
COMMENT     Sequence Label: pBbA20k-RFP
FEATURES             Location/Qualifiers
     source          1..2787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..41
                     /label=BBa_J23114
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.1"
     CDS             87..764
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      797..868
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      884..911
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(939..956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1073..1618)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1153..1172)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1732..1826)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1857..2651)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1968..1987)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2578..2597
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gacgtcttta tagctagctc agtcctaggt acaatgctag caggtttaat cgaattcaaa
       61 agatctttta agaaggagat atacatatgg cgagtagcga agacgttatc aaagagttca
      121 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg
      181 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg
      241 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt
      301 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca
      361 aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct
      421 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg
      481 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc
      541 cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc
      601 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg
      661 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg
      721 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaaggatcc aaactcgagt
      781 aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      841 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      901 gcctttctgc gtttatacct agggatatat tccgcttcct cgctcactga ctcgctacgc
      961 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1021 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1081 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1141 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1201 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1261 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1321 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1381 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1441 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     1501 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     1561 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     1621 agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa
     1681 tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc
     1741 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     1801 cattactgga tctatcaaca ggagtccaag cgagctctcg aaccccagag tcccgctcag
     1861 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     1921 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     1981 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     2041 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     2101 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     2161 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     2221 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     2281 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     2341 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     2401 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     2461 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     2521 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     2581 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     2641 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcatga tcccctgcgc
     2701 catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta
     2761 ccagagggcg ccccagctgg caattcc
//
