LOCUS       sequence-246989-       14251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Eukaryotic expression of the fusion protein used for sulfa-drugs 
            sensing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14251)
  AUTHORS   Scarabelli S, Tan KT, Griss R, Hovius R, D'Alessandro PL, Vorherr T,
            Johnsson K
  TITLE     Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins.
  JOURNAL   ACS Sens. 2017 Aug 25;2(8):1191-1197. doi: 
            10.1021/acssensors.7b00331. Epub 2017 Aug 2.
  PUBMED    28766337
REFERENCE   2  (bases 1 to 14251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.7b00331"; journalName: "ACS Sens"; date: 
            "2017-08-25- 25"; volume: "2"; issue: "8"; pages: "1191-1197"
COMMENT     Sequence Label: pEBTet-SNAP-HCA_sensor
FEATURES             Location/Qualifiers
     source          1..14251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..36
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             43..57
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             616..1329
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     CDS             join(1453..1455,1456..1458,1459..1971)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(1498..1519)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1759..1778)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2106..2127
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2962..2991
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(3032..3049)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3038..3149
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7985..8003)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8071..8175
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8176..8244,8245..9036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8394..8413)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9207..9795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9696..9715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(9984..10004)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10002..10299
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             10376..10975
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10376..10395)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10872..10892
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    11066..11200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11338..11357)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        11634..11895
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          12012..12584
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     12592..12611
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     12639..12658
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12639..12657
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             12668..13288
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(12741..12760)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    13330..13451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        13506..13885
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13886..14089
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     14040..14060
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    14091..14109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    14112..14130
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     14140..14164
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 accatggcaa gctggagcca cccgcagttc gaaaagggtg cagatgacga cgacaaggta
       61 ccaatggaca aggattgtga aatgaaacgc accacactgg acagcccttt ggggaagctg
      121 gagctgtctg gttgtgagca gggtctgcac gaaataattt ttctgggcaa ggggacgtct
      181 gcagctgatg ccgtggaggt cccagccccc gctgcggttc tcggaggtcc ggagcccctg
      241 atgcaggcta cagcctggct gaatgcctat ttccaccagc ccgaggctat cgaagagttc
      301 cccgtgccgg ctcttcacca tcccgttttc cagcaagagt cgttcaccag acaggtgtta
      361 tggaagctgc tgaaggttgt gaaattcgga gaagtgattt cttactctca cttagcagcc
      421 ctggcaggca accccgcagc cacggcagca gtgaagacgg cactgagtgg caatcctgtc
      481 cctatcctga tcccgtgcca cagagtggtc cagggggatt tggatgtggg cggttacgag
      541 ggtggactgg ccgtgaagga atggcttctg gcccatgaag gccaccggtt ggggaagcca
      601 ggcttgggaa ctagtatgtc taaaggtgaa gaattattca ctggtgttgt cccaattttg
      661 gttgaattag atggtgatgt taatggtcac aaattttctg tctccggtga aggtgaaggt
      721 gatgctacgt acggtaaatt gaccttaaaa ttactctgta ctactggtaa attgccagtt
      781 ccatggccaa ccttagtcac tactttaggt tatggtgttc aatgttttgc tagataccca
      841 gatcatatga aacaacatga ctttttcaag tctgccatgc cagaaggtta tgttcaagaa
      901 agaactattt ttttcaaaga tgacggtaac tacaagacca gagctgaagt caagtttgaa
      961 ggtgatacct tagttaatag aatcgaatta aaaggtattg attttaaaga agatggtaac
     1021 attttaggtc acaaattgga atacaactat aactctcaca atgtttacat cactgctgac
     1081 aaacaaaaga atggtatcaa agctaacttc aaaattagac acaacattga agatggtggt
     1141 gttcaattag ctgaccatta tcaacaaaat actccaattg gtgatggtcc agtcttgtta
     1201 ccagacaacc attacttatc ctatcaatct gccttattca aagatccaaa cgaaaagaga
     1261 gaccacatgg tcttgttaga atttttgact gctgctggta ttaccgaggg tatgaatgaa
     1321 ttgtacaaag aattccctcc tcctccgcca ccgccaccac cgccgccgcc gccaccacca
     1381 ggaggatctg gcggatctcc tcctccgcct cctccaccac caccgccacc gcctccacca
     1441 cctggcggcc gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1501 gtcgagctgg acggcgacgt aaacggccac aggttcagcg tgtccggcga gggcgagggc
     1561 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1621 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
     1681 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1741 cgtaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1801 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1861 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
     1921 aagcagaaga acggcatcaa ggcccacttc aagatccgcc acaacatcga ggacggcagc
     1981 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2041 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     2101 gatcacatgg tcctgctgga gttcgtgacc gccgccacta gtgccggagg catgtcccat
     2161 cactgggggt acggcaaaca caacggacct gagcactggc ataaggactt ccccattgcc
     2221 aagggagagc gccagtcccc tgttgacatc gacactcata cagccaagta tgacccttcc
     2281 ctgaagcccc tgtctgtttc ctatgatcaa gcaacttccc tgaggatcct caacaatggt
     2341 catgctttca acgtggagtt tgatgactct caggacaaag cagtgctcaa gggaggaccc
     2401 ctggatggca cttacagatt gattcagttt cactttcact ggggttcact tgatggacaa
     2461 ggttcagagc atactgtgga taaaaagaaa tatgctgcag aacttcactt ggttcactgg
     2521 aacaccaaat atggggattt tgggaaagct gtgcagcaac ctgatggact ggccgttcta
     2581 ggtatttttt tgaaggttgg cagcgctaaa ccgggccttc agaaagttgt tgacgtgctg
     2641 gattccatta aaacaaaggg caagagtgct gacttcacta acttcgatcc tcgtggcctc
     2701 cttcctgaat ccctggatta ctggacctac ccaggctcac tgaccacccc tcctcttctg
     2761 gaatgtgtga cctggattgt gctcaaggaa cccatcagcg tcagcagcga gcaggtgttg
     2821 aaattccgta aacttaactt caatggggag ggtgaacccg aagaactgat ggtggacaac
     2881 tggcgcccag ctcagccact gaagaacagg caaatcaaag cttccttcaa acgagctccg
     2941 ggcttctcct caatttccgc tcatcaccac catcatcacc atcaccacca ctaactcgag
     3001 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3061 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3121 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3181 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggctagag
     3241 gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg
     3301 gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca
     3361 catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta
     3421 taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag
     3481 tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc
     3541 aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg
     3601 tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata
     3661 gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt
     3721 atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc
     3781 tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc
     3841 tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca
     3901 caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct
     3961 tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta
     4021 tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg
     4081 gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata
     4141 aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg
     4201 ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat
     4261 aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca
     4321 ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc
     4381 agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg
     4441 gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg
     4501 cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg
     4561 gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg
     4621 caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc
     4681 gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc
     4741 tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact
     4801 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc
     4861 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     4921 taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
     4981 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5041 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5101 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     5161 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5221 ctgtatccgg gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta
     5281 gtagagtggg agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc
     5341 gctgcttgtc cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc
     5401 taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga
     5461 gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg
     5521 ttgggaggac gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga
     5581 tgtctactgg ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg
     5641 gcgtctccta acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct
     5701 tcatctccgt catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac
     5761 cagggaggca aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta
     5821 ggagcgggct ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata
     5881 tgagtttgta aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt
     5941 gggccgggtc caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt
     6001 ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat
     6061 acgaacacac cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc
     6121 aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc tccttgacca
     6181 cgatgctttc caaaccaccc tccttttttg cgcctgcctc catcaccctg accccggggt
     6241 ccagtgcttg ggccttctcc tgggtcatct gcggggccct gctctatcgc tcccgggggc
     6301 acgtcaggct caccatctgg gccaccttct tggtggtatt caaaataatc ggcttcccct
     6361 acagggtgga aaaatggcct tctacctgga gggggcctgc gcggtggaga cccggatgat
     6421 gatgactgac tactgggact cctgggcctc ttttctccac gtccacgacc tctccccctg
     6481 gctctttcac gacttccccc cctggctctt tcacgtcctc taccccggcg gcctccacta
     6541 cctcctcgac cccggcctcc actacctcct cgaccccggc ctccactgcc tcctcgaccc
     6601 cggcctccac ctcctgctcc tgcccctcct gctcctgccc ctcctcctgc tcctgcccct
     6661 cctgcccctc ctsctcctsc ccctcctccy sctcctsccc ctccycctcc tsccccyccc
     6721 sctcctsctc ctsctccycy tcctcctccw ccyccycctc cyscycctcc ycctcstcct
     6781 yyyycycctc cycckcctcc ycctccwcct cctcctcctc cycctcctcc gccscctccc
     6841 cctcctgctc ctcccccacc tcctgctcct gcccctcctg ctcctgcccc tcctgctcct
     6901 gcccctccts ctcctgcccc tcctscysct cctgcccctc ctgctcckcc tccacctcct
     6961 gctcctgccc ctcctgctcc tgcccctcct cctgctccts cccctcctgc ccctcctcct
     7021 cctccccctc ctcctyctcc tcctccycct cctccycctc ccccyccycc tcctcctccy
     7081 cctccyccyc cyccyccycc wccyccyccy ccyccycccc csccyccccc yccyccyccm
     7141 cccccsccyc cyccyccycc ycccccyccy ccysctccts cccctcctcc tsctcctgcc
     7201 cctcctgccc ctcctccngc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc
     7261 cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt
     7321 ggacgttttt ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc
     7381 ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta
     7441 tcaccccctc ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc
     7501 ttctctccta ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact
     7561 aaaagagatc aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact
     7621 cctgccccct ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc
     7681 caggtctgaa aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag
     7741 cccggaaaac tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc
     7801 aaattcctcg tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc
     7861 ttcctcttca aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc
     7921 ggcctgtgag gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
     7981 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     8041 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8101 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8161 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8221 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8281 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8341 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8401 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     8461 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8521 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8581 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8641 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8701 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8761 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8821 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8881 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8941 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9001 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9061 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9121 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9181 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9241 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9301 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     9361 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9421 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9481 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9541 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9601 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9661 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9721 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9781 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9841 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
     9901 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
     9961 gtcgaactag atgatccggc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
    10021 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
    10081 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    10141 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    10201 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    10261 ctctgagcta ttccagaagt agtgaggagg cttttttgga gggtgaccgc cacgaccggt
    10321 gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac cttccatgac
    10381 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
    10441 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc ccgaccgcca
    10501 catcgaacgc gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
    10561 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
    10621 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
    10681 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
    10741 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
    10801 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
    10861 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
    10921 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
    10981 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga cccggtccga cgcggcccga
    11041 cgggtccgag gggggtcgac ctcgaaactt gtttattgca gcttataatg gttacaaata
    11101 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    11161 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatccgaac cccttcctcg
    11221 accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct
    11281 cgaccaattc tcatgtttga cagcttatca tcgcagatcc gtatggtgca ctctcagtac
    11341 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
    11401 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
    11461 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
    11521 tacgcgtatc tgaggggact agggtgtgtt taggcgaaaa gcggggcttc ggttgtacgc
    11581 ggttaggagt cccctcagga tatagtagtt tcgcttttgc atagggaggg ggaaatgtag
    11641 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
    11701 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
    11761 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attccgcatt
    11821 gcagagatat tgtatttaag tgcctagctc gatacaataa acgccatttg accattcacc
    11881 acattggtgt gcacctccaa gctggccggg gatcctctag ggcctctgag ctattccaga
    11941 agtagtgaag aggctttttt ggaggcctag gcttttgcaa aaagctccgg atcgatcctg
    12001 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
    12061 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
    12121 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
    12181 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
    12241 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
    12301 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
    12361 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
    12421 gtggaaatat tcttattggt agaaacaact acatcctggt catcatcctg cctttctctt
    12481 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
    12541 gcccctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
    12601 tggttattgt gctgtctcat cattttggca aagaattgta atacgactca ctatagggcg
    12661 aattgatatg tctagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa
    12721 tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca
    12781 gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga
    12841 gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt
    12901 tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
    12961 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
    13021 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg
    13081 gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag
    13141 ggaaacacct actactgata gtatgccgcc attattacga caagctatcg aattatttga
    13201 tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga
    13261 aaaacaactt aaatgtgaaa gtgggtccgc gtacagcgga tcccgggaat tcagatctta
    13321 ttaaagcaga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    13381 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    13441 aatgtatctt atcatgtctg gtcgaatcca tggcgcagaa ctggtaggta tggaagatcc
    13501 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
    13561 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
    13621 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
    13681 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
    13741 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
    13801 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
    13861 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
    13921 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
    13981 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
    14041 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag
    14101 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
    14161 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
    14221 cagcctccgg actctagcgt ttaaacttaa g
//
