LOCUS       sequence-246999-        8547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Eukaryotic expression of PTK2B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Haruki H, Gonzalez MR, Johnsson K
  TITLE     Exploiting ligand-protein conjugates to monitor ligand-receptor 
            interactions.
  JOURNAL   PLoS One. 2012;7(5):e37598. doi: 10.1371/journal.pone.0037598. Epub 
            2012 May 31.
  PUBMED    22701522
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(5):e37598. doi"; pages: " 
            10.1371/journal.pone.0037598. Epub 2012 May 31"
COMMENT     Sequence Label: pCDNA3.1-V5-PTK2B
FEATURES             Location/Qualifiers
     source          1..8547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             914..955
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             956..976
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    1007..1031
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4095..4119)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4138..4155)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4144..4368
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4414..4842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4501..4520)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4711..4732
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4851..4871)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4856..5185
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5036..5171
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5098..5117
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5252..6046
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5306..5325)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5916..5935
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6220..6341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6257..6276)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6311..6330
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6390..6406)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6390..6406)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6403..6425)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6414..6430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6438..6444,6445..6462,6463..6468))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6483..6504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6621..6638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6792..7380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6872..6891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7551..8342,8343..8411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8174..8193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8412..8516)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttacc atgggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacggaaaa
      961 cctgtatttt cagggcccgg atccgtcgac gaattctgca gatatcacaa gtttgtacaa
     1021 aaaagcaggc ttggaaggag ttcgaaccat gtctggggtg tccgagcccc tgagtcgagt
     1081 aaagttgggc acgttacgcc ggcctgaagg ccctgcagag cccatggtgg tggtaccagt
     1141 agatgtggaa aaggaggacg tgcgtatcct caaggtctgc ttctatagca acagcttcaa
     1201 tcctgggaaa aacttcaaac tggtcaaatg cactgtccag acggagatcc gggagatcat
     1261 cacctccatc ctgctgagcg ggcggatcgg gcccaacatc cggttggctg agtgctatgg
     1321 gctgaggctg aagcacatga agtccgatga gatccactgg ctgcacccac agatgacagt
     1381 gggtgaggtg caggacaagt atgagtgtct gcacgtggaa gccgagtgga ggtatgacct
     1441 tcaaatccgc tacttgccag aagacttcat ggagagcctg aaggaggaca ggaccacgct
     1501 gctctatttt taccaacagc tccggaacga ctacatgcag cgctacgcca gcaaggtcag
     1561 cgagggcatg gccctgcagc tgggctgcct ggagctcagg cggttcttca aggatatgcc
     1621 ccacaatgca cttgacaaga agtccaactt cgagctccta gaaaaggaag tggggctgga
     1681 cttgtttttc ccaaagcaga tgcaggagaa cttaaagccc aaacagttcc ggaagatgat
     1741 ccagcagacc ttccagcagt acgcctcgct cagggaggag gagtgcgtca tgaagttctt
     1801 caacactctc gccggcttcg ccaacatcga ccaggagacc taccgctgtg aactcattca
     1861 aggatggaac attactgtgg acctggtcat tggccctaaa gggatccgcc agctgactag
     1921 tcaggacgca aagcccacct gcctggccga gttcaagcag atcaggtcca tcaggtgcct
     1981 cccgctggag gagggccagg cagtacttca gctgggcatt gaaggtgccc cccaggcctt
     2041 gtccatcaaa acctcatccc tagcagaggc tgagaacatg gctgacctca tagacggcta
     2101 ctgccggctg cagggtgagc accaaggctc tctcatcatc catcctagga aagatggtga
     2161 gaagcggaac agcctgcccc agatccccat gctaaacctg gaggcccggc ggtcccacct
     2221 ctcagagagc tgcagcatag agtcagacat ctacgcagag attcccgacg aaaccctgcg
     2281 aaggcccgga ggtccacagt atggcattgc ccgtgaagat gtggtcctga atcgtattct
     2341 tggggaaggc ttttttgggg aggtctatga aggtgtctac acaaatcata aaggggagaa
     2401 aatcaatgta gctgtcaaga cctgcaagaa agactgcact ctggacaaca aggagaagtt
     2461 catgagcgag gcagtgatca tgaagaacct cgaccacccg cacatcgtga agctgatcgg
     2521 catcattgaa gaggagccca cctggatcat catggaattg tatccctatg gggagctggg
     2581 ccactacctg gagcggaaca agaactccct gaaggtgctc accctcgtgc tgtactcact
     2641 gcagatatgc aaagccatgg cctacctgga gagcatcaac tgcgtgcaca gggacattgc
     2701 tgtccggaac atcctggtgg cctcccctga gtgtgtgaag ctgggggact ttggtctttc
     2761 ccggtacatt gaggacgagg actattacaa agcctctgtg actcgtctcc ccatcaaatg
     2821 gatgtcccca gagtccatta acttccgacg cttcacgaca gccagtgacg tctggatgtt
     2881 cgccgtgtgc atgtgggaga tcctgagctt tgggaagcag cccttcttct ggctggagaa
     2941 caaggatgtc atcggggtgc tggagaaagg agaccggctg cccaagcctg atctctgtcc
     3001 accggtcctt tataccctca tgacccgctg ctgggactac gaccccagtg accggccccg
     3061 cttcaccgag ctggtgtgca gcctcagtga cgtttatcag atggagaagg acattgccat
     3121 ggagcaagag aggaatgctc gctaccgaac ccccaaaatc ttggagccca cagccttcca
     3181 ggaaccccca cccaagccca gccgacctaa gtacagaccc cctccgcaaa ccaacctcct
     3241 ggctccaaag ctgcagttcc aggttcctga gggtctgtgt gccagctctc ctacgctcac
     3301 cagccctatg gagtatccat ctcccgttaa ctcactgcac accccacctc tccaccggca
     3361 caatgtcttc aaacgccaca gcatgcggga ggaggacttc atccaaccca gcagccgaga
     3421 agaggcccag cagctgtggg aggctgaaaa ggtcaaaatg cggcaaatcc tggacaaaca
     3481 gcagaagcag atggtggagg actaccagtg gctcaggcag gaggagaagt ccctggaccc
     3541 catggtttat atgaatgata agtccccatt gacgccagag aaggaggtcg gctacctgga
     3601 gttcacaggg cccccacaga agcccccgag gctgggcgca cagtccatcc agcccacagc
     3661 taacctggac cggaccgatg acctggtgta cctcaatgtc atggagctgg tgcgggccgt
     3721 gctggagctc aagaatgagc tctgtcagct gccccccgag ggctacgtgg tggtggtgaa
     3781 gaatgtgggg ctgaccctgc ggaagctcat cgggagcgtg gatgatctcc tgccttcctt
     3841 gccgtcatct tcacggacag agatcgaggg cacccagaaa ctgctcaaca aagacctggc
     3901 agagctcatc aacaagatgc ggctggcgca gcagaacgcc gtgacctccc tgagtgagga
     3961 gtgcaagagg cagatgctga cggcttcaca caccctggct gtggacgcca agaacctgct
     4021 cgacgctgtg gaccaggcca aggttctggc caatctggcc cacccacctg cagagtagct
     4081 cgagtgcggc cgcaacccag ctttcttgta caaagtggtg ataaacccgc tgatcagcct
     4141 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     4201 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     4261 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     4321 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     4381 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     4441 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4501 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4561 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4621 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4681 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4741 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4801 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     4861 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4921 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4981 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5041 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5101 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     5161 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     5221 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     5281 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5341 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5401 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     5461 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     5521 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     5581 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     5641 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     5701 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     5761 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     5821 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     5881 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     5941 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6001 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6061 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     6121 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6181 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     6241 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6301 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     6361 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6421 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6481 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6541 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6601 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6661 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6961 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7021 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7081 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7141 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7201 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7261 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7321 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7381 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7441 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7501 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7561 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7621 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7681 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7741 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7801 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7861 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7921 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7981 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8041 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8101 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8161 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8221 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8281 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8341 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8401 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8461 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8521 tttccccgaa aagtgccacc tgacgtc
//
