LOCUS       sequence-247028-       11893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To make virus expressing Doc2beta Halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Courtney NA, Briguglio JS, Bradberry MM, Greer C, Chapman ER
  TITLE     Excitatory and Inhibitory Neurons Utilize Different Ca(2+) Sensors 
            and Sources to Regulate Spontaneous Release.
  JOURNAL   Neuron. 2018 Jun 6;98(5):977-991.e5. doi: 
            10.1016/j.neuron.2018.04.022. Epub 2018 May 10.
  PUBMED    29754754
REFERENCE   2  (bases 1 to 11893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2018.04.022"; journalName: "Neuron"; date: 
            "2018-06-6- 6"; volume: "98"; issue: "5"; pages: "977-991.e5"
COMMENT     Sequence Label: pFSW Doc2beta Halotag
FEATURES             Location/Qualifiers
     source          1..11893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             4343..4363
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             4379..5260
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     misc_feature    5269..5840
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5290..5842
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5434..5451)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5661..5680
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5842..5844,5845..5847,5848..6561)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5842..5844,5845..5847,5848..6558)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5887..5908)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6148..6167)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6495..6516
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6585..7173
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6638..6658)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7056..7067)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7176..7192)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7177..7193)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7698..7878
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7904..7921)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7910..8134
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8180..8608
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8267..8286)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8477..8498
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8617..8637)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8622..8951
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8802..8937
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8864..8883
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8999..9046
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9065..9439
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9569..9690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9606..9625)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9660..9679
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9739..9755)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9739..9755)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9752..9774)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9763..9779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9787..9793,9794..9811,9812..9817))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9832..9853
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9970..9987)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10141..10729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10221..10240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10900..11691,11692..11760))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11523..11542
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11761..11865)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctaggc
     3061 caccatgacc ctccgaagac gtggagagaa ggctacaatc agcatccaag agcatatggc
     3121 aatcgacgtg tgtcctggac ctatccgacc tatcaagcag atctccgatt acttcccacg
     3181 attccctcgt ggactcccac caacagctgc accacgtgct tcagcacctc cagacgctcc
     3241 tgcacgatcg ccagcagcta ccgcaggtcc tcgtagcccc tccgacggag cacgcgacga
     3301 cgacgaagat gtggaccagc tcttcggagc ctacggtgcc agcccaggcc ccagccccgg
     3361 acccagcccc gtgaggccgc cggccaagcc ccccgaggac gaaccggacg ccgacggcta
     3421 cgagtcagac gactgcaccg ccctgggtac actggacttc agtctgctct atgaccagga
     3481 gaacaacgca ctgcactgca ccatcagcaa agccaagggc ctgaagccga tggatcacaa
     3541 tggactggct gatccctacg tcaaactaca cttgctgcct ggagccagca aggcaaataa
     3601 gctcagaaca aaaaccctac ggaacactct gaacccctcc tggaacgaga ccctcaccta
     3661 ttacgggatc acggatgagg acatgatccg aaagaccctg aggatctccg tgtgtgacga
     3721 ggacaaattc cgccacaacg agttcatcgg agagactcga gtgcccctga agaagctgaa
     3781 acccaaccac accaagacat tcagcatctg cctggagaag cagctgccgg tggacaaggc
     3841 agaggacaag tccctggagg agcgtggccg catcctcatc tctctcaagt acagctcaca
     3901 gaagcagggc ctgctggtgg gcatcgttcg ctgcgcacac ctggcggcca tggatgctaa
     3961 cggctactca gacccctacg tgaaaacata tctgaaacca gatgtagaca agaaatccaa
     4021 gcataagacc gcagtcaaga agaaaacact aaaccctgaa ttcaatgagg agttctgtta
     4081 cgagatcaag catggagacc tagccaaaaa gaccctggag gtcactgtct gggactatga
     4141 tattggaaaa tccaatgatt tcatcggtgg ggtggttctg ggcatcaatg ccaagggtga
     4201 gcgcctgaag cactggtttg actgtctgaa gaacaaggac aagaggattg agcgttggca
     4261 cacgctcacc aatgagatcc caggggctgt actcagcgac ggatccggaa gttcggggtc
     4321 cagcggttct tcaggcagtt ccgaggatct gtactttcag agcgataacg atggatccga
     4381 aatcggtact ggctttccat tcgaccccca ttatgtggaa gtcctgggcg agcgcatgca
     4441 ctacgtcgat gttggtccgc gcgatggcac ccctgtgctg ttcctgcacg gtaacccgac
     4501 ctcctcctac gtgtggcgca acatcatccc gcatgttgca ccgacccatc gctgcattgc
     4561 tccagacctg atcggtatgg gcaaatccga caaaccagac ctgggttatt tcttcgacga
     4621 ccacgtccgc ttcatggatg ccttcatcga agccctgggt ctggaagagg tcgtcctggt
     4681 cattcacgac tggggctccg ctctgggttt ccactgggcc aagcgcaatc cagagcgcgt
     4741 caaaggtatt gcatttatgg agttcatccg ccctatcccg acctgggacg aatggccaga
     4801 atttgcccgc gagaccttcc aggccttccg caccaccgac gtcggccgca agctgatcat
     4861 cgatcagaac gtttttatcg agggtacgct gccgatgggt gtcgtccgcc cgctgactga
     4921 agtcgagatg gaccattacc gcgagccgtt cctgaatcct gttgaccgcg agccactgtg
     4981 gcgcttccca aacgagctgc caatcgccgg tgagccagcg aacatcgtcg cgctggtcga
     5041 agaatacatg gactggctgc accagtcccc tgtcccgaag ctgctgttct ggggcacccc
     5101 aggcgttctg atcccaccgg ccgaagccgc tcgcctggcc aaaagcctgc ctaactgcaa
     5161 ggctgtggac atcggcccgg gtctgaatct gctgcaagaa gacaacccgg acctgatcgg
     5221 cagcgagatc gcgcgctggc tgtctactct ggagatttcc tgaaattccc cctctccctc
     5281 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     5341 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     5401 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     5461 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     5521 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     5581 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     5641 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     5701 cccagaaggt accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat
     5761 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     5821 tttgaaaaac acgatgataa tatggtgagc aagggcgagg agctgttcac cggggtggtg
     5881 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     5941 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     6001 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     6061 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     6121 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     6181 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     6241 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     6301 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     6361 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     6421 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     6481 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     6541 atggacgagc tgtacaagta aaattcgata tcaagcttat cgataatcaa cctctggatt
     6601 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6661 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6721 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6781 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6841 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6901 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6961 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     7021 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7081 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7141 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     7201 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     7261 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     7321 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7381 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     7441 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     7501 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     7561 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     7621 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     7681 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7741 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7801 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7861 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     7921 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7981 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     8041 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     8101 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     8161 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8221 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8281 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8341 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     8401 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8461 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8521 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8581 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     8641 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8701 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8761 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     8821 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8881 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8941 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     9001 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     9061 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     9121 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     9181 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     9241 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     9301 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     9361 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     9421 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     9481 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     9541 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     9601 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9661 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     9721 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9781 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9841 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9901 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9961 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10021 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10081 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10141 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10201 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10261 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10321 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10381 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10441 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10501 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10561 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    10621 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10681 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10741 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10801 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10861 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10921 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10981 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    11041 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    11101 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    11161 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    11221 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    11281 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    11341 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    11401 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    11461 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11521 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11581 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11641 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11701 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11761 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11821 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11881 agtgccacct gac
//
