LOCUS       sequence-247043-        2703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-conditional expression of the SthK channel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Bernal Sierra YA, Rost BR, Pofahl M, Fernandes AM, Kopton RA, Moser 
            S, Holtkamp D, Masala N, Beed P, Tukker JJ, Oldani S, Bonigk W, Kohl
            P, Baier H, Schneider-Warme F, Hegemann P, Beck H, Seifert R, 
            Schmitz D
  TITLE     Potassium channel-based optogenetic silencing.
  JOURNAL   Nat Commun. 2018 Nov 5;9(1):4611. doi: 10.1038/s41467-018-07038-8.
  PUBMED    30397200
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-5"; volume: "9(1):4611. doi"; pages: " 
            10.1038/s41467-018-07038-8"
COMMENT     Sequence Label: pAAV-Syn-FLEX-Sthk-P2A-bPAC_minWPRE
FEATURES             Location/Qualifiers
     source          1..2703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      159..614
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(246..265)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     456..477
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(631..650)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     750..772
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(810..828)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        896..1000
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1001..1069,1070..1861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1219..1238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2032..2620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2521..2540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcgctcact gaggccgccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg
       61 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
      121 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
      181 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
      241 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      301 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      361 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
      421 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
      481 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
      541 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
      601 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
      661 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
      721 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
      781 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      841 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
      901 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      961 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1021 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1081 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1141 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1201 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     1261 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1321 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     1381 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     1441 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     1501 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     1561 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     1621 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     1681 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     1741 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     1801 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     1861 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1921 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1981 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2041 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2101 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2161 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2221 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2281 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2341 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2401 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2461 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2521 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2581 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2641 tttacggttc ctggcctttt gctggccttt tgctcacatg tcctgcaggc agctgcgcgc
     2701 tcg
//
