LOCUS       sequence-247053-        5732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination. May not contain 
            entire coding region of gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5732)
  TITLE     SGC Toronto Q3-Q4 2018
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 6N31
FEATURES             Location/Qualifiers
     source          1..5732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             181..201
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    1338..1472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1375..1394)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1429..1448
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1501..1666
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1850..2304
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1937..1956)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2146..2167
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2330..2434
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2435..2503,2504..3295)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2653..2672)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3466..4054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3955..3974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4208..4225
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4359..4583
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4650..5183)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5353..5358,5359..5375,5376..5381))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg catcatcatc atcatcacag cagcggcaga
      181 gaaaacttgt atttccaggg caccgcaccc cagccagagc aatgcatgtt ccatgatgac
      241 tggatcagtt caattaaagg ggcagaggaa tggatcttga ctggttctta tgataagact
      301 tctcggatct ggtccttgga aggaaagtca ataatgacaa ttgtgggaca tacggatgtt
      361 gtaaaagatg tggcctgggt gaaaaaagat agtttgtcct gcttattatt gagtgcttct
      421 atggatcaga ctattctctt atgggagtgg aatgtagaga gaaacaaagt gaaagcccta
      481 cactgctgta gaggtcatgc tggaagtgta gattctatag ctgttgatgg ctcaggaact
      541 aaattttgca gtggctcctg ggataagatg ctaaagatct ggtctacagt ccctacagat
      601 gaagaagatg aaatggagga gtccacaaat cgaccaagaa agaaacagaa gacagaacag
      661 ttgggactaa caaggactcc catagtgacc ctctctggcc acatggaggc agtttcctca
      721 gttctgtggt cagatgctga agaaatctgc agtgcatctt gggaccatac aattagagtg
      781 tgggatgttg agtctggcag tcttaagtca actttgacag gaaataaagt gtttaattgt
      841 atttcctatt ctccactttg taaacgttta gcatctggaa gcacagatag gcatatcaga
      901 ctgtgggatc cccgaactaa agatggttct ttggtgtcgc tgtccctaac gtcacatact
      961 ggttgggtga catcagtaaa atggtctcct acccatgaac agcagctgat ttcaggatct
     1021 ttagataaca ttgttaagct gtgggataca agaagttgta aggctcctct ctatgatctg
     1081 gctgctcatg aagacaaagt tctgagtgta gactggacag acacagggct acttctgagt
     1141 ggaggagcag acaataaatt gtattcctac agatattcac ctaccacttc ccatgttggg
     1201 tgatgacgaa gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag
     1261 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     1321 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1381 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1441 aactcatcaa tgtatcttat catgtctgga tctgatcact gatatcgcct aggagatccg
     1501 aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct
     1561 tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa
     1621 ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg ggcatttttc
     1681 ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc gtcatcttcg
     1741 gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt attaatgttt
     1801 gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatggga cgcgccctgt
     1861 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     1921 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     1981 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2041 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     2101 tagacggttt ttcgcccttt gacgttggag tccacgttct taatagtgga ctcttgttcc
     2161 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     2221 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     2281 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2341 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2401 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2461 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2521 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2581 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2641 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2701 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2761 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2821 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2881 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2941 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     3001 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     3061 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     3121 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     3181 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3241 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3301 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3361 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3421 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3481 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3541 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3601 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3661 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3721 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3781 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3841 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3901 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3961 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4021 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4081 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4141 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4201 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     4261 tacgcatctg tgcggtattt cacaccgcat agaccagccg cgtaacctgg caaaatcggt
     4321 tacggttgag taataaatgg atgccctgcg taagcgggtg tgggcggaca ataaagtctt
     4381 aaactgaaca aaatagatct aaactatgac aataaagtct taaactagac agaatagttg
     4441 taaactgaaa tcagtccagt tatgctgtga aaaagcatac tggacttttg ttatggctaa
     4501 agcaaactct tcattttctg aagtgcaaat tgcccgtcgt attaaagagg ggcgtggcca
     4561 agggcatggt aaagactata ttcgcggcgt tgtgacaatt taccgaacaa ctccgcggcc
     4621 gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag
     4681 tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg
     4741 taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttgagcaga
     4801 ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc gagatcatag
     4861 atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca
     4921 acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc
     4981 ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga
     5041 ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg
     5101 cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta
     5161 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     5221 gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact
     5281 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
     5341 tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg tgccttcatc
     5401 cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg
     5461 tcctggctgg cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc attggcggcc
     5521 ttgctgttct tctacggcaa ggtgctgtgc acggatctgc cctggcttca ggagatcgga
     5581 agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt ggttcgcatc
     5641 ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg actctagcta tagttctagt
     5701 ggttggctac gtatactccg gaatattaat ag
//
