LOCUS       sequence-247065-        8713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET His6 MBP TEV CbAgo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Hegge JW, Swarts DC, Chandradoss SD, Cui TJ, Kneppers J, Jinek M, 
            Joo C, van der Oost J
  TITLE     DNA-guided DNA cleavage at moderate temperatures by Clostridium 
            butyricum Argonaute.
  JOURNAL   Nucleic Acids Res. 2019 May 9. pii: 5487266. doi: 
            10.1093/nar/gkz306.
  PUBMED    31069393
REFERENCE   2  (bases 1 to 8713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2019 May 9. pii: 5487266. doi: 10.1093/nar/gkz306."
COMMENT     Sequence Label: pJH-MBP-CbAgo
FEATURES             Location/Qualifiers
     source          1..8713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     106..125
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     209..228
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        209..227
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    228..252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             267..289
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             308..325
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             335..1435
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1409..1432
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1448..1468
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             3785..3802
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3855..3873)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3869..3916
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3953..4408
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4040..4059)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4250..4271
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4501..5316)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4501..5316)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5224..5243
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5308..5327
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5438..6026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5927..5946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6180..6197
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6212..6351
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6277..6296)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6437..6459
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6453..6644)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7453..8535)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8497..8516
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8536..8613)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
      181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
      241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat ataccatggg
      301 ttcttctcac catcaccatc accatggttc ttctatgaaa atcgaagaag gtaaactggt
      361 aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta agaaattcga
      421 gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag agaaattccc
      481 acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg accgctttgg
      541 tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt tccaggacaa
      601 gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat
      661 cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac
      721 ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga gcgcgctgat
      781 gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg ggggttatgc
      841 gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata acgctggcgc
      901 gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga atgcagacac
      961 cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg
     1021 cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa cggtactgcc
     1081 gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc
     1141 cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa aactatctgc tgactgatga
     1201 aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga agtcttacga
     1261 ggaagagttg gcgaaagatc cacgtattgc cgccactatg gaaaacgccc agaaaggtga
     1321 aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta ctgcggtgat
     1381 caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc agactaatgg
     1441 gatcgaggaa aacctgtact tccaatccaa tgcaaataat ctgacctttg aggcttttga
     1501 agggattggt caactgaatg aactgaattt ttataagtat cgtctgattg ggaaagggca
     1561 gattgataat gtgcatcaag ctatttggtc tgttaaatat aaactccagg ctaataattt
     1621 ttttaaaccg gtgtttgtga aaggtgagat tctatatagc ctagatgaac tgaaggtgat
     1681 tccggaattt gaaaatgttg aggtgattct cgatggtaat attattctgt ctatttctga
     1741 gaataccgat atttataagg atgtgattgt gttctatatt aacaatgctc tcaaaaatat
     1801 taaagatatt actaattatc gtaagtacat taccaaaaat accgatgaga ttatatgtaa
     1861 atctattctg actaccaatc tgaaatatca gtatatgaaa tctgaaaaag ggtttaaact
     1921 gcagaggaaa tttaaaatta gcccggttgt ttttcgtaat gggaaggtga ttctgtatct
     1981 caattgtagc agcgattttt ctaccgataa gtctatatat gagatgctaa atgatggtct
     2041 gggggtggtg ggtctacaag tgaaaaataa atggaccaat gcaaatggga atattttcat
     2101 tgaaaaagta ctggataata ccatttctga cccgggtact tctggtaagc taggtcagtc
     2161 tctaattgat tactatatta atggtaatca aaagtatcgt gttgaaaagt ttaccgatga
     2221 agataaaaat gcaaaggtga ttcaggctaa gattaagaat aaaacctata attatattcc
     2281 gcaagctctg accccggtga ttaccaggga atatctgagc cataccgata agaagttttc
     2341 taagcagatt gagaatgtga ttaaaatgga catgaattat cgttatcaga ccctaaaatc
     2401 tttcgttgaa gatattggtg tgattaaaga actcaataat ctgcatttta aaaatcagta
     2461 ttatactaat tttgatttta tggggtttga atctggtgtt ctggaagaac cggtactgat
     2521 gggggctaac ggtaaaatta aagataagaa gcaaattttt attaatgggt ttttcaagaa
     2581 tccgaaggag aatgtgaagt ttggtgttct gtatccggaa gggtgtatgg aaaatgctca
     2641 gtcgattgct cgttctatac tagattttgc aaccgctggg aaatataata aacaagagaa
     2701 taaatatatt agcaagaatc taatgaatat tggttttaag ccgtctgaat gtattttcga
     2761 atcttataaa ctcggtgata ttaccgaata taaagcaacc gctcgtaagc taaaagaaca
     2821 tgaaaaggtg ggttttgtga ttgcagtgat tccggatatg aatgaactgg aagtggaaaa
     2881 tccgtataat ccgtttaaaa aagtatgggc aaaactgaat attccgagcc agatgattac
     2941 cctgaaaacc accgaaaaat ttaaaaatat tgttgataag tctggtctat attatctaca
     3001 caatattgct ctcaatattc tagggaaaat tggggggatt ccgtggatta ttaaagatat
     3061 gccgggtaat attgattgtt tcattgggct agatgtgggt acccgtgaaa aaggtattca
     3121 ttttccggca tgtagcgttc tatttgataa atatgggaaa ctgattaatt attataagcc
     3181 gaccattccg cagtctggtg agaaaattgc agaaaccatt ctgcaagaga ttttcgataa
     3241 tgttctgatt agctataaag aagaaaatgg ggaatatccg aaaaatattg tgattcatcg
     3301 tgatgggttt tctcgtgaaa atattgattg gtataaagaa tattttgata agaaagggat
     3361 taaatttaac attattgagg tgaaaaagaa tattccggtt aaaattgcaa aggtggttgg
     3421 gtcgaacatt tgtaatccca ttaaaggttc ttatgttctc aaaaatgata aggcttttat
     3481 tgttaccacc gatattaaag atggggttgc tagcccgaac cccctgaaaa ttgaaaagac
     3541 ctatggtgac gtggagatga aaagcattct agaacagatt tatagcctgt ctcaaattca
     3601 tgttgggagc accaagtctc taaggctccc gattaccacc ggttatgctg ataaaatttg
     3661 caaagcaatt gagtacattc cgcaaggtgt tgttgataat cgtctattct ttctgtaata
     3721 acattggaag tggataacgg atccgaattc gagcgccgtc gacaagcttg cggccgcact
     3781 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
     3841 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     3901 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
     3961 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4021 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4081 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4141 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4201 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4261 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4321 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4381 tttaacaaac tagtaacgtt tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga
     4441 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc
     4501 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     4561 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     4621 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     4681 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     4741 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
     4801 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     4861 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     4921 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaatg ttttcacctg aatcaggata
     4981 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
     5041 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     5101 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     5161 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
     5221 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     5281 cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     5341 gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact
     5401 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5461 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5521 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5581 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5641 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5701 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5761 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5821 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5881 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5941 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6001 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6061 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6121 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6181 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     6241 tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata
     6301 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     6361 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6421 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6481 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
     6541 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
     6601 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
     6661 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
     6721 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
     6781 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat
     6841 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata
     6901 atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc
     6961 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
     7021 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
     7081 aacgacagga gcacgatcat gcgcacccgt ggggccgcca tgccggcgat aatggcctgc
     7141 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
     7201 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
     7261 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
     7321 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     7381 aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta
     7441 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7501 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct
     7561 tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg
     7621 cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa
     7681 cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc
     7741 accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt
     7801 ggcaaccagc atcgcagtgg gaacgatacc ctcattcagc atttgcatgg tttgttgaaa
     7861 accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt
     7921 gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc
     7981 taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc
     8041 gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa
     8101 cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata
     8161 gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc
     8221 ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg
     8281 agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac
     8341 gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt
     8401 cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg
     8461 gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa
     8521 cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat
     8581 accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg
     8641 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
     8701 ggaatggtgc atg
//
