LOCUS       sequence-247076-       15397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15397)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX097
FEATURES             Location/Qualifiers
     source          1..15397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5511..5535)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5564..5653
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5687..5939
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6368..6620)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7088..8113)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7088..8113)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8123..8467)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8609..8626)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8609..8625)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8618..8640)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8765..8784)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8975..8996
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9243..9267
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10863..12011)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12310..13104)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13296..14063
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14756..15387
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg gctggtccag aattgttcta
     4621 cggcgatgct gctggtttta atggtattct gcttcagaac cagaggctta tgtcttcatt
     4681 tgctacaaaa gaggctgttg cttctcctcg ctattcccct tccttccttg cctccggttc
     4741 catggtcagc tttcgtgttg taaatggagg gaacaaatca gataggtcat tgtttcagtc
     4801 agtcgaagag aatgaaacag atgagttgga ggaatattta aatcaaccag agaaaaagag
     4861 gcgacttaca gctaaccaag tacagtttct tgagaagagt tttgaggagg tgaacaaact
     4921 ggagccagag agaaaagacc agcttgctaa agagctcggt ttgcaacctc gccaaatagc
     4981 gatttggttt cagaatcgtc gtgcacgatg gaggacaaaa cagcttgaga ataattacga
     5041 ggcgctgaga aataaatatg acaatctgaa agcagactac atcaatcttc tcaaagagaa
     5101 ggatgatcta agagctcagg ttagccagtt tactgatatg ctgctacaca aggagaaaga
     5161 aaagggggaa ctggctactt ctgatataaa ggaactttcc gatgcatcgc caaaagaaaa
     5221 catgattgat cagaaagtgt cagctattac acttaagcag cacaaagact tgaattctct
     5281 tcaaagtgat atgtttgatt cagaaagact ggtgcaaccc tctatcttag atcagggtga
     5341 ttcttcttat gcatttgaac ctgaccagtc agatttatcc caagatgaag aagaaaactt
     5401 gagcaagaat ctggttgtac aagatcatta tcccatgaca tcctcaaact tgagctgctt
     5461 tggatattca gttgaagatc agggctttgg tttctggtct tttcccgggg acccagcttt
     5521 cttgtacaaa gtggttgata acagcgggtt aattaacatc ttttacccat acgatgttcc
     5581 tgactatgcg ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt
     5641 tccagattac gctgctcagt gcagcgctta gagctcgaat ttccccgatc gttcaaacat
     5701 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     5761 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     5821 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     5881 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     5941 ggaattagct tcatcaacgc aagacatgcg cacgaccgtc tgacaggaga ggaatttccg
     6001 acgagcacag aaaggacttg ctcttggacg taggcctatt tctcaggcac atgtatcaag
     6061 tgttcggacg tgggttttcg atggtgtatc agccgccgcc aactgggaga tgaggaggct
     6121 ttcttggggg gcagtcagca gttcatttca caagacagag gaacttgtaa ggagatgcac
     6181 tgatttatct tggcgcaaac cagcaggacg aattagtggg aatagcccgc gaatatctaa
     6241 gttatgcctg tcggcatgag cagaaacttc caattcgaaa cagtttggag aggttgtttt
     6301 tgggcatacc ttttgttagt cagcctctcg attgctcatc gtcattacac agtaccgaag
     6361 tttgatcgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
     6421 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
     6481 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
     6541 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
     6601 tgccaaatgt ttgaacgatc tgcttcgacg cactccttct ttactccacc atctcgtcct
     6661 tattgaaaac gtgggtagca ccaaaacgaa tcaagtcgct ggaactgaag ttaccaatca
     6721 cgctggatga tttgccagtt ggattaatct tgcctttccc cgcatgaata atattgatga
     6781 atgcatgcgt gaggggtatt tcgattttgg caatagctgc aattgccgcg acatcctcca
     6841 acgagcataa ttcttcagaa aaatagcgat gttccatgtt gtcagggcat gcatgatgca
     6901 cgttatgagg tgacggtgct aggcagtatt ccctcaaagt ttcatagtca gtatcatatt
     6961 catcattgca ttcctgcaag agagaattga gacgcaatcc acacgctgcg gcaaccttcc
     7021 ggcgttcgtg gtctatttgc tcttggacgt tgcaaacgta agtgttggat cccggtcggc
     7081 atctactcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     7141 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     7201 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     7261 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     7321 acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     7381 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     7441 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     7501 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     7561 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     7621 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     7681 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     7741 ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt
     7801 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
     7861 cgctgaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     7921 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     7981 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     8041 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     8101 caggcttttt catatcgggg tcgtcctctc caaatgaaat gaacttcctt atatagagga
     8161 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
     8221 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
     8281 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
     8341 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
     8401 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
     8461 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
     8521 agtgtcgtgc tccaccatgt tgacggatct ctaggacgcg tcgacgcgtc gacgcgtcga
     8581 cgcgtcgacg cgtcctagaa gctaattcac tggccgtcgt tttacaacgt cgtgactggg
     8641 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     8701 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     8761 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8821 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8881 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8941 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9001 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa
     9061 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
     9121 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
     9181 aaaccacccc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
     9241 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     9301 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
     9361 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
     9421 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
     9481 gacagagcgt tgctgcctgt gatcaaatat catctccctc gcagagatcc gaattatcag
     9541 ccttcttatt catttctcgc ttaaccgtga caggctgtcg atcttgagaa ctatgccgac
     9601 ataataggaa atcgctggat aaagccgctg aggaagctga gtggcgctat ttctttagaa
     9661 gtgaacgttg acgatatcaa ctcccctatc cattgctcac cgaatggtac aggtcgggga
     9721 cccgaagttc cgactgtcgg cctgatgcat ccccggctga tcgaccccag atctggggct
     9781 gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc ggaaccaaag
     9841 gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa cattggttcc
     9901 tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca gaagtcctcc
     9961 ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc acttgcgggt
    10021 cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga cgccgacagg
    10081 atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc cgcaaatagc
    10141 ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga tgaacacgac
    10201 catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc ggtagaccga
    10261 aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga agatgcgcat
    10321 ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac ccgccggcaa
    10381 cgcccgcagc agcataccgg cgacccctcg gcctcgctgt tcgggctcca cgaaaacgcc
    10441 ggacagatgc gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga ccgacagccc
    10501 aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc gttcagcgaa
    10561 cgcctccatg ggctttttct cctcgtgctc gtaaacggac ccgaacatct ctggagcttt
    10621 cttcagggcc gacaatcgga tctcgcggaa atcctgcacg tcggccgctc caagccgtcg
    10681 aatctgagcc ttaatcacaa ttgtcaattt taatcctctg tttatcggca gttcgtagag
    10741 cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt
    10801 tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg
    10861 ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg
    10921 cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa
    10981 tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag
    11041 ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg
    11101 aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg
    11161 caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat
    11221 tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg
    11281 cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc
    11341 tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc
    11401 tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc
    11461 acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg
    11521 ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc
    11581 ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt
    11641 ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt
    11701 tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct
    11761 gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca
    11821 gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag
    11881 ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg
    11941 gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac
    12001 gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct
    12061 tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg
    12121 aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc
    12181 tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc ctgagagcca
    12241 aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac
    12301 atctaggtac taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg
    12361 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct
    12421 gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag
    12481 atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc
    12541 gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg
    12601 cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt
    12661 attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc
    12721 cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc
    12781 agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag
    12841 cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc
    12901 ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg
    12961 gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat ataccttagc
    13021 aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc
    13081 cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag
    13141 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
    13201 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
    13261 gtatcgacgg agccgatttt gaaaccacaa ttatgggtga tgctgccaac ttactgattt
    13321 agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc
    13381 tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat
    13441 ctctgctctc actgccgtaa aacatggcaa ctgcagttca cttacaccgc ttctcaaccc
    13501 ggtacgcacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg ggcaacagcc
    13561 cgcattatgg gcgttggcct caacacgatt ttacgtcact taaaaaactc aggccgcagt
    13621 cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac
    13681 agtggggcta tgtcggggct aaatcgcgcc agcgctggct gttttacgcg tatgacagtc
    13741 tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc
    13801 ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc tggccgctgt
    13861 atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg cagcgaattg
    13921 agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag tcgctgtcgt
    13981 tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac ataaaacact
    14041 atcaataagt tggagtcatt acccaattat gatagaattt acaagctata aggttattgt
    14101 cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat
    14161 tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg atatcttcta
    14221 tataaaagat atattatctt atcagtattg tcaatatatt caaggcaatc tgcctcctca
    14281 tcctcttcat cctcttcgtc ttggtagctt tttaaatatg gcgcttcata gagtaattct
    14341 gtaaaggtcc aattctcgtt ttcatacctc ggtataatct tacctatcac ctcaaatggt
    14401 tcgctgggtt tatcgcaccc ccgaacacga gcacggcacc cgcgaccact atgccaagaa
    14461 tgcccaaggt aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg acggccgaag
    14521 tgaagggcag gccgccaccc aggccgccgc cctcactgcc cggcacctgg tcgctgaatg
    14581 tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg ctgatcgccc
    14641 atcccgttac tgccccgatc ccggcaatgg caaggactgc cagcgctgcc atttttgggg
    14701 tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg
    14761 gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg
    14821 gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca
    14881 ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg
    14941 gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt
    15001 gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg
    15061 gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt
    15121 tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca
    15181 tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg
    15241 tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca
    15301 cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg
    15361 gcgagggcaa ccagcccggt gagcgtcgga aaggcgc
//
