LOCUS       sequence-247081-       15326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15326)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX099
FEATURES             Location/Qualifiers
     source          1..15326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5472..5496)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5525..5614
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5648..5900
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6329..6581)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7049..8074)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7049..8074)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8084..8428)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8538..8555)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8538..8554)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8547..8569)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8694..8713)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8904..8925
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9172..9196
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10792..11940)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12239..13033)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLLLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13225..13992
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14685..15316
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgggatc
     4621 tgggcatata tttttcgacc cgtcttgtca cggcaacatg ttgttccttg ggaattcaga
     4681 tcctgttttc cgaggatctt cgatgatgaa gatggagaac tcctcaaaga agcgaccttt
     4741 ctttagctcg ccggaggaac tatatgacga ggaatattac gacgagcagt cgccggagaa
     4801 gaaacgccgt ctcactcccg agcaggtgca cttgctggag aagagctttg agacggagaa
     4861 caagctggag ccagagcgta agactcagct agccaagaag cttggcctgc agcccaggca
     4921 ggtggctgtg tggttccaaa accgccgtgc ccggtggaag accaagacgc ttgaaagaga
     4981 ttatgatcag ctcaaatcct cttatgactc tcttctctct gattttgact ccatgcgcaa
     5041 agataatgag aagctcaaag ctgaggtggt ttcattgatg gagaagttgc aggggaaagt
     5101 ggttggtgaa ggaggggtaa caagtgaaaa atgtgaggtt ttggaggtag acgctatgac
     5161 ccttcaagtg aaggtgaagg cggaggacag gctgagcagt ggcagcggcg gaagcgcggt
     5221 ggtagatgaa cacagtccac agctggtgga cagtggggac tcctattttc acactggtca
     5281 tattgaagag tatccaatag gggctggagg atgcaataat attcctccac ctatggatgg
     5341 tttgcagtca gaggaggatg atggtagtga tgatggcggc ggccgtggct acttctctaa
     5401 tgtctttgtg gcggcagagg agcagcaaca tgaagaagga gagcctattg gatggttctg
     5461 gtctcccggg gacccagctt tcttgtacaa agtggttgat aacagcgggt taattaacat
     5521 cttttaccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc cggactatgc
     5581 aggatcctat ccatatgacg ttccagatta cgctgctcag tgcagcgctt agagctcgaa
     5641 tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     5701 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     5761 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     5821 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     5881 gtcatctatg ttactagatc gggaattagc ttcatcaacg caagacatgc gcacgaccgt
     5941 ctgacaggag aggaatttcc gacgagcaca gaaaggactt gctcttggac gtaggcctat
     6001 ttctcaggca catgtatcaa gtgttcggac gtgggttttc gatggtgtat cagccgccgc
     6061 caactgggag atgaggaggc tttcttgggg ggcagtcagc agttcatttc acaagacaga
     6121 ggaacttgta aggagatgca ctgatttatc ttggcgcaaa ccagcaggac gaattagtgg
     6181 gaatagcccg cgaatatcta agttatgcct gtcggcatga gcagaaactt ccaattcgaa
     6241 acagtttgga gaggttgttt ttgggcatac cttttgttag tcagcctctc gattgctcat
     6301 cgtcattaca cagtaccgaa gtttgatcga tctagtaaca tagatgacac cgcgcgcgat
     6361 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     6421 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     6481 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     6541 ttcaatctta agaaacttta ttgccaaatg tttgaacgat ctgcttcgac gcactccttc
     6601 tttactccac catctcgtcc ttattgaaaa cgtgggtagc accaaaacga atcaagtcgc
     6661 tggaactgaa gttaccaatc acgctggatg atttgccagt tggattaatc ttgcctttcc
     6721 ccgcatgaat aatattgatg aatgcatgcg tgaggggtat ttcgattttg gcaatagctg
     6781 caattgccgc gacatcctcc aacgagcata attcttcaga aaaatagcga tgttccatgt
     6841 tgtcagggca tgcatgatgc acgttatgag gtgacggtgc taggcagtat tccctcaaag
     6901 tttcatagtc agtatcatat tcatcattgc attcctgcaa gagagaattg agacgcaatc
     6961 cacacgctgc ggcaaccttc cggcgttcgt ggtctatttg ctcttggacg ttgcaaacgt
     7021 aagtgttgga tcccggtcgg catctactct attcctttgc cctcggacga gtgctggggc
     7081 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc
     7141 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc
     7201 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
     7261 caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc
     7321 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga
     7381 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg
     7441 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc
     7501 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg
     7561 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc
     7621 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc
     7681 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca
     7741 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg
     7801 agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga
     7861 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc
     7921 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt
     7981 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
     8041 cgacagacgt cgcggtgagt tcaggctttt tcatatcggg gtcgtcctct ccaaatgaaa
     8101 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
     8161 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     8221 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     8281 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
     8341 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
     8401 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
     8461 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttgacggatc tctaggacgc
     8521 gtcctagaag ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     8581 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8641 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt
     8701 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8761 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8821 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8881 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8941 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
     9001 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     9061 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccacccca
     9121 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     9181 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     9241 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     9301 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     9361 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     9421 gctgcctgtg atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc
     9481 atttctcgct taaccgtgac aggctgtcga tcttgagaac tatgccgaca taataggaaa
     9541 tcgctggata aagccgctga ggaagctgag tggcgctatt tctttagaag tgaacgttga
     9601 cgatatcaac tcccctatcc attgctcacc gaatggtaca ggtcggggac ccgaagttcc
     9661 gactgtcggc ctgatgcatc cccggctgat cgaccccaga tctggggctg agaaagccca
     9721 gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta
     9781 aacccgctcc gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg
     9841 ggattggcgg atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt
     9901 gccaggcggt aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc
     9961 cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg
    10021 cgtttggtgt caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg
    10081 ccatcaatcg tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct
    10141 gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca
    10201 agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc
    10261 ccgttggaat ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca
    10321 gcataccggc gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg
    10381 ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc
    10441 cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg
    10501 gctttttctc ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg
    10561 acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct
    10621 taatcacaat tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg
    10681 tcccgagcga tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc
    10741 cagtaaagcg ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt
    10801 gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc
    10861 ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca
    10921 catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc
    10981 gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta
    11041 gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt
    11101 tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc
    11161 aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc
    11221 cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt
    11281 gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac
    11341 cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt
    11401 gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt
    11461 gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc
    11521 aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc
    11581 ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt
    11641 ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg
    11701 ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag
    11761 ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg
    11821 gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc
    11881 ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat
    11941 tcaccctcct tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt
    12001 tgacccgcct ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc
    12061 gtagaccgtc tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac
    12121 cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat
    12181 gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggtact
    12241 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
    12301 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
    12361 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
    12421 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
    12481 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
    12541 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
    12601 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
    12661 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
    12721 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
    12781 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagcaata
    12841 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
    12901 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
    12961 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
    13021 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
    13081 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
    13141 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
    13201 gccgattttg aaaccacaat tatgggtgat gctgccaact tactgattta gtgtatgatg
    13261 gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac
    13321 tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca
    13381 ctgccgtaaa acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca
    13441 gaaaatcatt gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg
    13501 cgttggcctc aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg
    13561 cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat
    13621 gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg
    13681 gttgttgcgc acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg
    13741 ctgtcaccct ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc
    13801 ctgaagggaa agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac
    13861 ctgaatctga ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg
    13921 gtggagctgc atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt
    13981 ggagtcatta cccaattatg atagaattta caagctataa ggttattgtc ctgggtttca
    14041 agcattagtc catgcaagtt tttatgcttt gcccattcta tagatatatt gataagcgcg
    14101 ctgcctatgc cttgccccct gaaatcctta catacggcga tatcttctat ataaaagata
    14161 tattatctta tcagtattgt caatatattc aaggcaatct gcctcctcat cctcttcatc
    14221 ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag agtaattctg taaaggtcca
    14281 attctcgttt tcatacctcg gtataatctt acctatcacc tcaaatggtt cgctgggttt
    14341 atcgcacccc cgaacacgag cacggcaccc gcgaccacta tgccaagaat gcccaaggta
    14401 aaaattgccg gccccgccat gaagtccgtg aatgccccga cggccgaagt gaagggcagg
    14461 ccgccaccca ggccgccgcc ctcactgccc ggcacctggt cgctgaatgt cgatgccagc
    14521 acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc tgatcgccca tcccgttact
    14581 gccccgatcc cggcaatggc aaggactgcc agcgctgcca tttttggggt gaggccgttc
    14641 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt
    14701 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg
    14761 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg
    14821 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc
    14881 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg
    14941 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc
    15001 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg
    15061 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc
    15121 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc
    15181 caagttttcc gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga
    15241 cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac
    15301 cagcccggtg agcgtcggaa aggcgc
//
