LOCUS       sequence-247088-       15358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15358)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX101
FEATURES             Location/Qualifiers
     source          1..15358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5472..5496)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5525..5614
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5648..5900
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6329..6581)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7049..8074)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7049..8074)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8084..8428)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8570..8587)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8570..8586)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8579..8601)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8726..8745)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8936..8957
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9204..9228
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10824..11972)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12271..13065)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13257..14024
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14717..15348
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgggatc
     4621 tgggcatata tttttcgacc cgtcttgtca cggcaacatg ttgttccttg ggaattcaga
     4681 tcctgttttc cgaggatctt cgatgatgaa gatggagaac tcctcaaaga agcgaccttt
     4741 ctttagctcg ccggaggaac tatatgacga ggaatattac gacgagcagt cgccggagaa
     4801 gaaacgccgt ctcactcccg agcaggtgca cttgctggag aagagctttg agacggagaa
     4861 caagctggag ccagagcgta agactcagct agccaagaag cttggcctgc agcccaggca
     4921 ggtggctgtg tggttccaaa accgccgtgc ccggtggaag accaagacgc ttgaaagaga
     4981 ttatgatcag ctcaaatcct cttatgactc tcttctctct gattttgact ccatgcgcaa
     5041 agataatgag aagctcaaag ctgaggtggt ttcattgatg gagaagttgc aggggaaagt
     5101 ggttggtgaa ggaggggtaa caagtgaaaa atgtgaggtt ttggaggtag acgctatgac
     5161 ccttcaagtg aaggtgaagg cggaggacag gctgagcagt ggcagcggcg gaagcgcggt
     5221 ggtagatgaa cacagtccac agctggtgga cagtggggac tcctattttc acactggtca
     5281 tattgaagag tatccaatag gggctggagg atgcaataat attcctccac ctatggatgg
     5341 tttgcagtca gaggaggatg atggtagtga tgatggcggc ggccgtggct acttctctaa
     5401 tgtctttgtg gcggcagagg agcagcaaca tgaagaagga gagcctattg gatggttctg
     5461 gtctcccggg gacccagctt tcttgtacaa agtggttgat aacagcgggt taattaacat
     5521 cttttaccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc cggactatgc
     5581 aggatcctat ccatatgacg ttccagatta cgctgctcag tgcagcgctt agagctcgaa
     5641 tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     5701 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     5761 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     5821 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     5881 gtcatctatg ttactagatc gggaattagc ttcatcaacg caagacatgc gcacgaccgt
     5941 ctgacaggag aggaatttcc gacgagcaca gaaaggactt gctcttggac gtaggcctat
     6001 ttctcaggca catgtatcaa gtgttcggac gtgggttttc gatggtgtat cagccgccgc
     6061 caactgggag atgaggaggc tttcttgggg ggcagtcagc agttcatttc acaagacaga
     6121 ggaacttgta aggagatgca ctgatttatc ttggcgcaaa ccagcaggac gaattagtgg
     6181 gaatagcccg cgaatatcta agttatgcct gtcggcatga gcagaaactt ccaattcgaa
     6241 acagtttgga gaggttgttt ttgggcatac cttttgttag tcagcctctc gattgctcat
     6301 cgtcattaca cagtaccgaa gtttgatcga tctagtaaca tagatgacac cgcgcgcgat
     6361 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     6421 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     6481 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     6541 ttcaatctta agaaacttta ttgccaaatg tttgaacgat ctgcttcgac gcactccttc
     6601 tttactccac catctcgtcc ttattgaaaa cgtgggtagc accaaaacga atcaagtcgc
     6661 tggaactgaa gttaccaatc acgctggatg atttgccagt tggattaatc ttgcctttcc
     6721 ccgcatgaat aatattgatg aatgcatgcg tgaggggtat ttcgattttg gcaatagctg
     6781 caattgccgc gacatcctcc aacgagcata attcttcaga aaaatagcga tgttccatgt
     6841 tgtcagggca tgcatgatgc acgttatgag gtgacggtgc taggcagtat tccctcaaag
     6901 tttcatagtc agtatcatat tcatcattgc attcctgcaa gagagaattg agacgcaatc
     6961 cacacgctgc ggcaaccttc cggcgttcgt ggtctatttg ctcttggacg ttgcaaacgt
     7021 aagtgttgga tcccggtcgg catctactct attcctttgc cctcggacga gtgctggggc
     7081 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc
     7141 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc
     7201 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
     7261 caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc
     7321 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga
     7381 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg
     7441 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc
     7501 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg
     7561 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc
     7621 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc
     7681 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca
     7741 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg
     7801 agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga
     7861 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc
     7921 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt
     7981 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
     8041 cgacagacgt cgcggtgagt tcaggctttt tcatatcggg gtcgtcctct ccaaatgaaa
     8101 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
     8161 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     8221 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     8281 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
     8341 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
     8401 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
     8461 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttgacggatc tctaggacgc
     8521 gtcgacgcgt cgacgcgtcg acgcgtcgac gcgtcctaga agctaattca ctggccgtcg
     8581 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     8641 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     8701 agttgcgcag cctgaatggc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8761 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8821 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     8881 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8941 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     9001 gggattttgc cgatttcgga accaccatca aacaggattt tcgcctgctg gggcaaacca
     9061 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
     9121 ccgtctcact ggtgaaaaga aaaaccaccc cagtacatta aaaacgtccg caatgtgtta
     9181 ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa
     9241 cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc
     9301 cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg
     9361 ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg
     9421 tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaaata tcatctccct
     9481 cgcagagatc cgaattatca gccttcttat tcatttctcg cttaaccgtg acaggctgtc
     9541 gatcttgaga actatgccga cataatagga aatcgctgga taaagccgct gaggaagctg
     9601 agtggcgcta tttctttaga agtgaacgtt gacgatatca actcccctat ccattgctca
     9661 ccgaatggta caggtcgggg acccgaagtt ccgactgtcg gcctgatgca tccccggctg
     9721 atcgacccca gatctggggc tgagaaagcc cagtaaggaa acaactgtag gttcgagtcg
     9781 cgagatcccc cggaaccaaa ggaagtaggt taaacccgct ccgatcaggc cgagccacgc
     9841 caggccgaga acattggttc ctgtaggcat cgggattggc ggatcaaaca ctaaagctac
     9901 tggaacgagc agaagtcctc cggccgccag ttgccaggcg gtaaaggtga gcagaggcac
     9961 gggaggttgc cacttgcggg tcagcacggt tccgaacgcc atggaaaccg cccccgccag
    10021 gcccgctgcg acgccgacag gatctagcgc tgcgtttggt gtcaacacca acagcgccac
    10081 gcccgcagtt ccgcaaatag cccccaggac cgccatcaat cgtatcgggc tacctagcag
    10141 agcggcagag atgaacacga ccatcagcgg ctgcacagcg cctaccgtgg ccgcgacccc
    10201 gcccggcagg cggtagaccg aaataaacaa caagctccag aatagcgaaa tattaagtgc
    10261 gccgaggatg aagatgcgca tccaccagat tcccgttgga atctgtcgga cgatcatcac
    10321 gagcaataaa cccgccggca acgcccgcag cagcataccg gcgacccctc ggcctcgctg
    10381 ttcgggctcc acgaaaacgc cggacagatg cgccttgtga gcgtccttgg ggccgtcctc
    10441 ctgtttgaag accgacagcc caatgatctc gccgtcgatg taggcgccga atgccacggc
    10501 atctcgcaac cgttcagcga acgcctccat gggctttttc tcctcgtgct cgtaaacgga
    10561 cccgaacatc tctggagctt tcttcagggc cgacaatcgg atctcgcgga aatcctgcac
    10621 gtcggccgct ccaagccgtc gaatctgagc cttaatcaca attgtcaatt ttaatcctct
    10681 gtttatcggc agttcgtaga gcgcgccgtg cgtcccgagc gatactgagc gaagcaagtg
    10741 cgtcgagcag tgcccgcttg ttcctgaaat gccagtaaag cgctggctgc tgaaccccca
    10801 gccggaactg accccacaag gccctagcgt ttgcaatgca ccaggtcatc attgacccag
    10861 gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc
    10921 cacttcttca cgcgggtgga atccgatccg cacatgaggc ggaaggtttc cagcttgagc
    10981 gggtacggct cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc
    11041 ttgcggtact tctcccatat gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt
    11101 tcctcgtcga tcaggacctg gcaacgggac gttttcttgc cacggtccag gacgcggaag
    11161 cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc
    11221 gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt aataccggcc attgatcgac
    11281 cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg
    11341 cgcttcgcgt actccaacac ctgctgccac accagttcgt catcgtcggc ccgcagctcg
    11401 acgccggtgt aggtgatctt cacgtccttg ttgacgtgga aaatgacctt gttttgcagc
    11461 gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt
    11521 ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga acaaggaaag ctgcatttcc
    11581 ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct gacctgtttt
    11641 gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg
    11701 gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg
    11761 gccgatggcg cgggcagggc agggggagcc agttgcacgc tgtcgcgctc gatcttggcc
    11821 gtagcttgct ggaccatcga gccgacggac tggaaggttt cgcggggcgc acgcatgacg
    11881 gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc
    11941 ctgtatgcct tccggtcaaa cgtccgattc attcaccctc cttgcgggat tgccccgact
    12001 cacgccgggg caatgtgccc ttattcctga tttgacccgc ctggtgcctt ggtgtccaga
    12061 taatccacct tatcggcaat gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac
    12121 ttggtattcc gaatcttgcc ctgcacgaat accagcgacc ccttgcccaa atacttgccg
    12181 tgggcctcgg cctgagagcc aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg
    12241 tcgccggcat cgttgcgcca catctaggta ctaaaacaat tcatccagta aaatataata
    12301 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
    12361 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
    12421 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
    12481 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
    12541 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
    12601 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
    12661 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
    12721 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
    12781 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
    12841 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
    12901 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
    12961 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
    13021 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
    13081 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
    13141 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
    13201 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccaca attatgggtg
    13261 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct
    13321 gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc
    13381 accgccggac atcagcgcta tctctgctct cactgccgta aaacatggca actgcagttc
    13441 acttacaccg cttctcaacc cggtacgcac cagaaaatca ttgatatggc catgaatggc
    13501 gttggatgcc gggcaacagc ccgcattatg ggcgttggcc tcaacacgat tttacgtcac
    13561 ttaaaaaact caggccgcag tcggtaacct cgcgcataca gccgggcagt gacgtcatcg
    13621 tctgcgcgga aatggacgaa cagtggggct atgtcggggc taaatcgcgc cagcgctggc
    13681 tgttttacgc gtatgacagt ctccggaaga cggttgttgc gcacgtattc ggtgaacgca
    13741 ctatggcgac gctggggcgt cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga
    13801 tgacggatgg ctggccgctg tatgaatccc gcctgaaggg aaagctgcac gtaatcagca
    13861 agcgatatac gcagcgaatt gagcggcata acctgaatct gaggcagcac ctggcacggc
    13921 tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct gcatgacaaa gtcatcgggc
    13981 attatctgaa cataaaacac tatcaataag ttggagtcat tacccaatta tgatagaatt
    14041 tacaagctat aaggttattg tcctgggttt caagcattag tccatgcaag tttttatgct
    14101 ttgcccattc tatagatata ttgataagcg cgctgcctat gccttgcccc ctgaaatcct
    14161 tacatacggc gatatcttct atataaaaga tatattatct tatcagtatt gtcaatatat
    14221 tcaaggcaat ctgcctcctc atcctcttca tcctcttcgt cttggtagct ttttaaatat
    14281 ggcgcttcat agagtaattc tgtaaaggtc caattctcgt tttcatacct cggtataatc
    14341 ttacctatca cctcaaatgg ttcgctgggt ttatcgcacc cccgaacacg agcacggcac
    14401 ccgcgaccac tatgccaaga atgcccaagg taaaaattgc cggccccgcc atgaagtccg
    14461 tgaatgcccc gacggccgaa gtgaagggca ggccgccacc caggccgccg ccctcactgc
    14521 ccggcacctg gtcgctgaat gtcgatgcca gcacctgcgg cacgtcaatg cttccgggcg
    14581 tcgcgctcgg gctgatcgcc catcccgtta ctgccccgat cccggcaatg gcaaggactg
    14641 ccagcgctgc catttttggg gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg
    14701 atgggaggcc cgcgttagcg ggccgggagg gttcgagaag ggggggcacc ccccttcggc
    14761 gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat aaatattggt
    14821 ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa acccttgcaa
    14881 atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg
    14941 tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc
    15001 ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat ctgtgggaaa
    15061 ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc
    15121 cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg
    15181 tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc
    15241 cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca
    15301 tagacggccg ccagcccagc ggcgagggca accagcccgg tgagcgtcgg aaaggcgc
//
