LOCUS       sequence-247089-       16687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16687)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX102
FEATURES             Location/Qualifiers
     source          1..16687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6801..6825)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6854..6943
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6977..7229
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7658..7910)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8378..9403)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8378..9403)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9413..9757)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9899..9916)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9899..9915)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9908..9930)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10055..10074)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10265..10286
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10533..10557
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12153..13301)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13600..14394)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14586..15353
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16046..16677
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgtttca
     4621 gcaaaccatg ttcgatagcc acaatttttt gctagatttg agccataaaa catcagaaaa
     4681 cgagatggac ttgattcgag atgaagaatt cgagagcaaa tcaggcgctg ataccacgga
     4741 gttagcccct tcaggagacg atcaagatcc taataaacgt cccaagaaaa agcaatacca
     4801 tcggcataca caacatcaaa tccaagaatt ggaagcgttt ttcaaagaat gccctcatcc
     4861 agatgataaa caaaggaaag agttggggaa aagattaggg ttagagcctt tgcaaattaa
     4921 gttttggttc caaaacaagc gtactcaaat gaaggctcat cacgaacgcc acgagaacac
     4981 acaattgagg aacgagaatg agaaactacg tgccgagaat atacggtata aagaagccct
     5041 tagtactgca acatgtccaa actgtggagg gccggctgcc ataggcgaga tgtcatttga
     5101 tgaacagcac ttgaggattg aaaatgcccg tctaagagaa gagattgaca ggatatctgg
     5161 gattgcagca aaatatgttg ggaaaccaat gcttaattat cctcaacttc cttctcctat
     5221 accaccaact cgttcacttg atcttggagt ggcaaatttt gttcatcaat taggagaaat
     5281 ttacaatgct ggtgaccttc ttagatcaat ttctggctca acagatgccg ataaaccaat
     5341 gataattgag cttgctgttg cagcaatgga ggaattaatc agattggctg aaactagaga
     5401 gcccttatgg attcaaagtt cggaaaattc cgttgagatt ttaagtgagg aggaatatgc
     5461 tcggacattt cctcgagggc ttggaccaaa gtcacttgga ttaaaatctg aagcgtcacg
     5521 agaatcggcc gtagtcatta tgaatcacat taatttagtg gaaattttga tggatgtgaa
     5581 ccaatggaca agttttttcg ctgggctagt gtccaaagca acgacgttgg aagtcttatc
     5641 gactggcgtc gcaggaaact acaatggagc tttgctagtt atgacagctg agttccaagt
     5701 cccctcacca cttgttccaa ctcgtgaaaa ctattttgta agatattgca aacaacatgc
     5761 tgatggaact tgggcagtag ttgatgtttc cttagacagt ttacgcccaa cttcagtagt
     5821 gtcacgttgt agaagaaggc catctggttg cctaattcga gaattaccaa atggttactc
     5881 caaggtaacg tggatcgaac acgttgaggt ggacgataga gctgtcaaca gtatttatag
     5941 acctcttgtg aattcaggcc tggcatttgg tgctaaaaga tgggtagcaa cattagatag
     6001 acaatgcgaa cgacttgcta gtgtaatggc caataacatc ccaactggag atgtcattac
     6061 aagtccagaa ggtagaaaaa gtatgttaaa acttgctgag agaatggtga tgagcttttg
     6121 cgcgggcgtt ggtgcatcaa cggctcacac atggacaaca ttatccggaa gtggtgctga
     6181 tgatgtgaga gtgatgacta ggaagagtat agatgatcca ggaagacctc ctggtattgt
     6241 actgagtgca gctacttcat tttggttgcc agttcctact aagagagtat ttgactttct
     6301 tcgcaatgag aattcaagaa atgagtggga tattctttcg aatggtggcc tagctcaaga
     6361 aatggcacac attgcaaatg gtcgtgaatc tggcaacagt gtctctctat tgcgcgttaa
     6421 tagtggaaac tcaagccaga ataatatgtt gatactccaa gagagttgca ctgattcaac
     6481 cggctcgtac gttatttacg cgccggttga tatagcagca atgaatgtgg tgctaagtgg
     6541 cggcgatcct gactatgtgg ctcttttgcc ttctggtttt gctatactcc ctgatggttc
     6601 tgctactact aatggtgttg gaattaatcc tgagagtaat gctgcgggtg gttcattact
     6661 cactgttgca tttcagatat tagttgattc agtcccaact gcgaaactat cgcttggatc
     6721 agttgcaaca gttaatagtc tcatcaaatg cactgttgaa aggatcaaag cttccatagt
     6781 atgcgaaagc gcacccgggg acccagcttt cttgtacaaa gtggttgata acagcgggtt
     6841 aattaacatc ttttacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc
     6901 ggactatgca ggatcctatc catatgacgt tccagattac gctgctcagt gcagcgctta
     6961 gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     7021 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     7081 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     7141 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     7201 gcgcgcggtg tcatctatgt tactagatcg ggaattagct tcatcaacgc aagacatgcg
     7261 cacgaccgtc tgacaggaga ggaatttccg acgagcacag aaaggacttg ctcttggacg
     7321 taggcctatt tctcaggcac atgtatcaag tgttcggacg tgggttttcg atggtgtatc
     7381 agccgccgcc aactgggaga tgaggaggct ttcttggggg gcagtcagca gttcatttca
     7441 caagacagag gaacttgtaa ggagatgcac tgatttatct tggcgcaaac cagcaggacg
     7501 aattagtggg aatagcccgc gaatatctaa gttatgcctg tcggcatgag cagaaacttc
     7561 caattcgaaa cagtttggag aggttgtttt tgggcatacc ttttgttagt cagcctctcg
     7621 attgctcatc gtcattacac agtaccgaag tttgatcgat ctagtaacat agatgacacc
     7681 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     7741 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     7801 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
     7861 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttcgacg
     7921 cactccttct ttactccacc atctcgtcct tattgaaaac gtgggtagca ccaaaacgaa
     7981 tcaagtcgct ggaactgaag ttaccaatca cgctggatga tttgccagtt ggattaatct
     8041 tgcctttccc cgcatgaata atattgatga atgcatgcgt gaggggtatt tcgattttgg
     8101 caatagctgc aattgccgcg acatcctcca acgagcataa ttcttcagaa aaatagcgat
     8161 gttccatgtt gtcagggcat gcatgatgca cgttatgagg tgacggtgct aggcagtatt
     8221 ccctcaaagt ttcatagtca gtatcatatt catcattgca ttcctgcaag agagaattga
     8281 gacgcaatcc acacgctgcg gcaaccttcc ggcgttcgtg gtctatttgc tcttggacgt
     8341 tgcaaacgta agtgttggat cccggtcggc atctactcta ttcctttgcc ctcggacgag
     8401 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
     8461 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
     8521 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
     8581 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
     8641 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
     8701 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
     8761 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
     8821 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
     8881 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
     8941 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
     9001 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
     9061 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
     9121 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
     9181 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
     9241 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
     9301 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
     9361 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatcgggg tcgtcctctc
     9421 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
     9481 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     9541 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     9601 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     9661 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     9721 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     9781 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tgacggatct
     9841 ctaggacgcg tcgacgcgtc gacgcgtcga cgcgtcgacg cgtcctagaa gctaattcac
     9901 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     9961 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    10021 cttcccaaca gttgcgcagc ctgaatggcg cccgctcctt tcgctttctt cccttccttt
    10081 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    10141 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
    10201 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    10261 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
    10321 gatttataag ggattttgcc gatttcggaa ccaccatcaa acaggatttt cgcctgctgg
    10381 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
    10441 agctgttgcc cgtctcactg gtgaaaagaa aaaccacccc agtacattaa aaacgtccgc
    10501 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
    10561 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
    10621 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
    10681 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
    10741 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaaatat
    10801 catctccctc gcagagatcc gaattatcag ccttcttatt catttctcgc ttaaccgtga
    10861 caggctgtcg atcttgagaa ctatgccgac ataataggaa atcgctggat aaagccgctg
    10921 aggaagctga gtggcgctat ttctttagaa gtgaacgttg acgatatcaa ctcccctatc
    10981 cattgctcac cgaatggtac aggtcgggga cccgaagttc cgactgtcgg cctgatgcat
    11041 ccccggctga tcgaccccag atctggggct gagaaagccc agtaaggaaa caactgtagg
    11101 ttcgagtcgc gagatccccc ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc
    11161 gagccacgcc aggccgagaa cattggttcc tgtaggcatc gggattggcg gatcaaacac
    11221 taaagctact ggaacgagca gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag
    11281 cagaggcacg ggaggttgcc acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc
    11341 ccccgccagg cccgctgcga cgccgacagg atctagcgct gcgtttggtg tcaacaccaa
    11401 cagcgccacg cccgcagttc cgcaaatagc ccccaggacc gccatcaatc gtatcgggct
    11461 acctagcaga gcggcagaga tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc
    11521 cgcgaccccg cccggcaggc ggtagaccga aataaacaac aagctccaga atagcgaaat
    11581 attaagtgcg ccgaggatga agatgcgcat ccaccagatt cccgttggaa tctgtcggac
    11641 gatcatcacg agcaataaac ccgccggcaa cgcccgcagc agcataccgg cgacccctcg
    11701 gcctcgctgt tcgggctcca cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg
    11761 gccgtcctcc tgtttgaaga ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa
    11821 tgccacggca tctcgcaacc gttcagcgaa cgcctccatg ggctttttct cctcgtgctc
    11881 gtaaacggac ccgaacatct ctggagcttt cttcagggcc gacaatcgga tctcgcggaa
    11941 atcctgcacg tcggccgctc caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt
    12001 taatcctctg tttatcggca gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg
    12061 aagcaagtgc gtcgagcagt gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct
    12121 gaacccccag ccggaactga ccccacaagg ccctagcgtt tgcaatgcac caggtcatca
    12181 ttgacccagg cgtgttccac caggccgctg cctcgcaact cttcgcaggc ttcgccgacc
    12241 tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc
    12301 agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc
    12361 ggcgacagct tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc
    12421 acgacgattt cctcgtcgat caggacctgg caacgggacg ttttcttgcc acggtccagg
    12481 acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag
    12541 cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta ataccggcca
    12601 ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg
    12661 ataggggtgc gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc
    12721 cgcagctcga cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg
    12781 ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc
    12841 gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa caaggaaagc
    12901 tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg
    12961 acctgttttg ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat agttcctcgc
    13021 gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct gttcgagacg acgcgaacgc
    13081 tccacggcgg ccgatggcgc gggcagggca gggggagcca gttgcacgct gtcgcgctcg
    13141 atcttggccg tagcttgctg gaccatcgag ccgacggact ggaaggtttc gcggggcgca
    13201 cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc
    13261 agttcttgcc tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt
    13321 gccccgactc acgccggggc aatgtgccct tattcctgat ttgacccgcc tggtgccttg
    13381 gtgtccagat aatccacctt atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc
    13441 ttctcgtact tggtattccg aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa
    13501 tacttgccgt gggcctcggc ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc
    13561 tcctgcttgt cgccggcatc gttgcgccac atctaggtac taaaacaatt catccagtaa
    13621 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
    13681 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
    13741 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
    13801 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
    13861 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
    13921 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
    13981 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
    14041 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
    14101 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
    14161 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
    14221 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
    14281 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
    14341 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
    14401 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
    14461 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
    14521 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccacaa
    14581 ttatgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca
    14641 gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac
    14701 ggcaaaagca ccgccggaca tcagcgctat ctctgctctc actgccgtaa aacatggcaa
    14761 ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc
    14821 atgaatggcg ttggatgccg ggcaacagcc cgcattatgg gcgttggcct caacacgatt
    14881 ttacgtcact taaaaaactc aggccgcagt cggtaacctc gcgcatacag ccgggcagtg
    14941 acgtcatcgt ctgcgcggaa atggacgaac agtggggcta tgtcggggct aaatcgcgcc
    15001 agcgctggct gttttacgcg tatgacagtc tccggaagac ggttgttgcg cacgtattcg
    15061 gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg
    15121 tgatatggat gacggatggc tggccgctgt atgaatcccg cctgaaggga aagctgcacg
    15181 taatcagcaa gcgatatacg cagcgaattg agcggcataa cctgaatctg aggcagcacc
    15241 tggcacggct gggacggaag tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag
    15301 tcatcgggca ttatctgaac ataaaacact atcaataagt tggagtcatt acccaattat
    15361 gatagaattt acaagctata aggttattgt cctgggtttc aagcattagt ccatgcaagt
    15421 ttttatgctt tgcccattct atagatatat tgataagcgc gctgcctatg ccttgccccc
    15481 tgaaatcctt acatacggcg atatcttcta tataaaagat atattatctt atcagtattg
    15541 tcaatatatt caaggcaatc tgcctcctca tcctcttcat cctcttcgtc ttggtagctt
    15601 tttaaatatg gcgcttcata gagtaattct gtaaaggtcc aattctcgtt ttcatacctc
    15661 ggtataatct tacctatcac ctcaaatggt tcgctgggtt tatcgcaccc ccgaacacga
    15721 gcacggcacc cgcgaccact atgccaagaa tgcccaaggt aaaaattgcc ggccccgcca
    15781 tgaagtccgt gaatgccccg acggccgaag tgaagggcag gccgccaccc aggccgccgc
    15841 cctcactgcc cggcacctgg tcgctgaatg tcgatgccag cacctgcggc acgtcaatgc
    15901 ttccgggcgt cgcgctcggg ctgatcgccc atcccgttac tgccccgatc ccggcaatgg
    15961 caaggactgc cagcgctgcc atttttgggg tgaggccgtt cgcggccgag gggcgcagcc
    16021 cctgggggga tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc
    16081 cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata
    16141 aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa
    16201 cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc
    16261 cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta
    16321 gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc
    16381 tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac
    16441 gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc
    16501 cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat
    16561 ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt
    16621 gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt gagcgtcgga
    16681 aaggcgc
//
