LOCUS       sequence-24709-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Vorvis C, Markus SM, Lee WL
  TITLE     Photoactivatable GFP tagging cassettes for protein-tracking studies 
            in the budding yeast Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2008 Sep;25(9):651-9.
  PUBMED    18727145
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2008-09"; volume: "25(9)"; pages: "651-9"
COMMENT     Sequence Label: pDH3-PA-GFP
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(29..31,32..34,35..547)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(74..95)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(335..354)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctactngtng ctgcaggtcg acggatccat ggtgagcaag ggcgaggagc tgttcaccgg
       61 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      121 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      181 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcagctacg gcgtgcagtg
      241 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      301 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      361 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      421 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      481 ctatatcatg gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
      541 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      601 cggccccgtg ctgctgcccg acaaccacta cctgagccac cagtcgnccc tgagcaaaga
      661 cccaacgaga cgcgcgatca catggtcttg ctganttngt gaaccgccgc ngatnntctc
      721 tcggctgang actntacagt aaaattgttn atttgctcgt cccgcggtca ccggcacgan
      781 tgagccaaat acctctnaan tntnatggnn cnanggggat gnngngccct tntnacctct
      841 cntgnttctt ntc
//
