LOCUS       sequence-247090-       15335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15335)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX103
FEATURES             Location/Qualifiers
     source          1..15335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5481..5505)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5534..5623
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5657..5909
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6338..6590)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7058..8083)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7058..8083)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8093..8437)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8547..8564)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8547..8563)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8556..8578)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8703..8722)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8913..8934
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9181..9205
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10801..11949)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12248..13042)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13234..14001
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14694..15325
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgatggt
     4621 tgagaaagaa gatttggggt tgagtcttag cctcagtttt ccagataata acaagaatag
     4681 tacaaccaat aatcctttgc aacatcatca gctcaatctc atcccttcat cttcaccttt
     4741 taatctcttt cacaaaactt cttggactga ttctttccct tcttcagatc ggaactcgga
     4801 gacatgcaga gtggagacaa ggacatttct gaaggtaata gacgtgaacc ggctgccggc
     4861 gacggcggac gccgatgaag aagccggcgt atcatcacct aataacagca ccatttcgag
     4921 tgtgagtgga aataagagaa gcgaaagaga gacaaataac tgtgaggagc atgaaataga
     4981 aagggcttct gatgaagaag acggagaaac ttctagaaag aaacttcggc tttcaaaaga
     5041 ccaatctgcc attctcgaag agactttcaa agaacacaac actctcaatc ctaaacaaaa
     5101 gctggctttg gctaaaagac tgggattgag acctaggcaa gttgaggtct ggtttcagaa
     5161 caggagggca aggacaaagt tgaagcaaac agaagttgac tgcgaatttt tgaagagatg
     5221 ttgtgagaat ttgacagagg aaaaccggag attactgaag gaagttcaag agttgagggc
     5281 acttaagctt tcacctcaat tctacatgca aatgactcct cctaccaccc ttaccatgtg
     5341 tccttcatgt gagcgtgtcg ggggcccctc aacctcatcc ggtcccacat cttcacatgt
     5401 gggcccggcc caacataggc ccatgccatt taacctttgg gccgacactc cacaatccct
     5461 tcacccgaga tctcccgggg acccagcttt cttgtacaaa gtggttgata acagcgggtt
     5521 aattaacatc ttttacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc
     5581 ggactatgca ggatcctatc catatgacgt tccagattac gctgctcagt gcagcgctta
     5641 gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     5701 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     5761 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     5821 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     5881 gcgcgcggtg tcatctatgt tactagatcg ggaattagct tcatcaacgc aagacatgcg
     5941 cacgaccgtc tgacaggaga ggaatttccg acgagcacag aaaggacttg ctcttggacg
     6001 taggcctatt tctcaggcac atgtatcaag tgttcggacg tgggttttcg atggtgtatc
     6061 agccgccgcc aactgggaga tgaggaggct ttcttggggg gcagtcagca gttcatttca
     6121 caagacagag gaacttgtaa ggagatgcac tgatttatct tggcgcaaac cagcaggacg
     6181 aattagtggg aatagcccgc gaatatctaa gttatgcctg tcggcatgag cagaaacttc
     6241 caattcgaaa cagtttggag aggttgtttt tgggcatacc ttttgttagt cagcctctcg
     6301 attgctcatc gtcattacac agtaccgaag tttgatcgat ctagtaacat agatgacacc
     6361 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     6421 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     6481 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
     6541 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttcgacg
     6601 cactccttct ttactccacc atctcgtcct tattgaaaac gtgggtagca ccaaaacgaa
     6661 tcaagtcgct ggaactgaag ttaccaatca cgctggatga tttgccagtt ggattaatct
     6721 tgcctttccc cgcatgaata atattgatga atgcatgcgt gaggggtatt tcgattttgg
     6781 caatagctgc aattgccgcg acatcctcca acgagcataa ttcttcagaa aaatagcgat
     6841 gttccatgtt gtcagggcat gcatgatgca cgttatgagg tgacggtgct aggcagtatt
     6901 ccctcaaagt ttcatagtca gtatcatatt catcattgca ttcctgcaag agagaattga
     6961 gacgcaatcc acacgctgcg gcaaccttcc ggcgttcgtg gtctatttgc tcttggacgt
     7021 tgcaaacgta agtgttggat cccggtcggc atctactcta ttcctttgcc ctcggacgag
     7081 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
     7141 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
     7201 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
     7261 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
     7321 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
     7381 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
     7441 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
     7501 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
     7561 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
     7621 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
     7681 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
     7741 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
     7801 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
     7861 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
     7921 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
     7981 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
     8041 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatcgggg tcgtcctctc
     8101 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
     8161 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     8221 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     8281 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     8341 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     8401 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     8461 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tgacggatct
     8521 ctaggacgcg tcctagaagc taattcactg gccgtcgttt tacaacgtcg tgactgggaa
     8581 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     8641 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgcc
     8701 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8761 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     8821 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     8881 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     8941 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggaacc
     9001 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
     9061 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
     9121 accaccccag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     9181 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     9241 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     9301 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     9361 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     9421 cagagcgttg ctgcctgtga tcaaatatca tctccctcgc agagatccga attatcagcc
     9481 ttcttattca tttctcgctt aaccgtgaca ggctgtcgat cttgagaact atgccgacat
     9541 aataggaaat cgctggataa agccgctgag gaagctgagt ggcgctattt ctttagaagt
     9601 gaacgttgac gatatcaact cccctatcca ttgctcaccg aatggtacag gtcggggacc
     9661 cgaagttccg actgtcggcc tgatgcatcc ccggctgatc gaccccagat ctggggctga
     9721 gaaagcccag taaggaaaca actgtaggtt cgagtcgcga gatcccccgg aaccaaagga
     9781 agtaggttaa acccgctccg atcaggccga gccacgccag gccgagaaca ttggttcctg
     9841 taggcatcgg gattggcgga tcaaacacta aagctactgg aacgagcaga agtcctccgg
     9901 ccgccagttg ccaggcggta aaggtgagca gaggcacggg aggttgccac ttgcgggtca
     9961 gcacggttcc gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat
    10021 ctagcgctgc gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg caaatagccc
    10081 ccaggaccgc catcaatcgt atcgggctac ctagcagagc ggcagagatg aacacgacca
    10141 tcagcggctg cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa
    10201 taaacaacaa gctccagaat agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc
    10261 accagattcc cgttggaatc tgtcggacga tcatcacgag caataaaccc gccggcaacg
    10321 cccgcagcag cataccggcg acccctcggc ctcgctgttc gggctccacg aaaacgccgg
    10381 acagatgcgc cttgtgagcg tccttggggc cgtcctcctg tttgaagacc gacagcccaa
    10441 tgatctcgcc gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg
    10501 cctccatggg ctttttctcc tcgtgctcgt aaacggaccc gaacatctct ggagctttct
    10561 tcagggccga caatcggatc tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa
    10621 tctgagcctt aatcacaatt gtcaatttta atcctctgtt tatcggcagt tcgtagagcg
    10681 cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc
    10741 ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc
    10801 ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc
    10861 tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc
    10921 cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct
    10981 gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa
    11041 tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca
    11101 acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc
    11161 caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca
    11221 ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc
    11281 gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg
    11341 ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac
    11401 gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt
    11461 gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg
    11521 ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt
    11581 cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt
    11641 tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc
    11701 cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg
    11761 gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc
    11821 gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc
    11881 atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt
    11941 ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta
    12001 ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa
    12061 gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg
    12121 cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa
    12181 acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat
    12241 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
    12301 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
    12361 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
    12421 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
    12481 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
    12541 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
    12601 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
    12661 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
    12721 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
    12781 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
    12841 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
    12901 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
    12961 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
    13021 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
    13081 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
    13141 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
    13201 atcgacggag ccgattttga aaccacaatt atgggtgatg ctgccaactt actgatttag
    13261 tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg
    13321 ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct
    13381 ctgctctcac tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg
    13441 tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg caacagcccg
    13501 cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg
    13561 gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag
    13621 tggggctatg tcggggctaa atcgcgccag cgctggctgt tttacgcgta tgacagtctc
    13681 cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct ggggcgtctt
    13741 atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat
    13801 gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag
    13861 cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc
    13921 tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat
    13981 caataagttg gagtcattac ccaattatga tagaatttac aagctataag gttattgtcc
    14041 tgggtttcaa gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg
    14101 ataagcgcgc tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata
    14161 taaaagatat attatcttat cagtattgtc aatatattca aggcaatctg cctcctcatc
    14221 ctcttcatcc tcttcgtctt ggtagctttt taaatatggc gcttcataga gtaattctgt
    14281 aaaggtccaa ttctcgtttt catacctcgg tataatctta cctatcacct caaatggttc
    14341 gctgggttta tcgcaccccc gaacacgagc acggcacccg cgaccactat gccaagaatg
    14401 cccaaggtaa aaattgccgg ccccgccatg aagtccgtga atgccccgac ggccgaagtg
    14461 aagggcaggc cgccacccag gccgccgccc tcactgcccg gcacctggtc gctgaatgtc
    14521 gatgccagca cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat
    14581 cccgttactg ccccgatccc ggcaatggca aggactgcca gcgctgccat ttttggggtg
    14641 aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc
    14701 cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc
    14761 gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg
    14821 ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga
    14881 cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt
    14941 caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc
    15001 acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc
    15061 gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc
    15121 tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc
    15181 agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca
    15241 cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc
    15301 gagggcaacc agcccggtga gcgtcggaaa ggcgc
//
