LOCUS       sequence-247094-       15085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15085)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX106
FEATURES             Location/Qualifiers
     source          1..15085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5199..5223)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5252..5341
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5375..5627
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6056..6308)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6776..7801)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6776..7801)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7811..8155)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8297..8314)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8297..8313)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8306..8328)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8453..8472)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8663..8684
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8931..8955
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10551..11699)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11998..12792)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12984..13751
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14444..15075
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg cctcgaccaa gatggagtcc
     4621 aacaccacaa cagctgatga ttttacaaga tttgtataga aaagggttga gaaatccaac
     4681 ttcttctcaa gtacaaaaga tcacaggtca cctttctctt tttggaaaga ttcaatgcaa
     4741 gaatgttttt tactggtttc aaaatctcaa agctagagac aggcaaaagc taagaaaaca
     4801 gctgcttcac caaatagaga aaaaccctac taaagataat gataatactc aattacctct
     4861 tttcatcaac aacaccaaca gcaacgacgt cgttcacttc gttgataatt atccttcttt
     4921 tcttcctaat gtccatacac tttaccctca ttctcctact atacttcttc atcagactga
     4981 aggaaaggag acatcgtcag ctcaaatgat gaacaacgtt gggaaagtgg atcttcgttt
     5041 aacagagaat gatatgatga gaacaaatat tcaaggttgg gtattgatga tgagagacaa
     5101 tatgggtcaa atttctattc cctgttgcag caacgttaga cccctcgaaa ccctacaact
     5161 cttccctgtc aaagaaaccg gagttaagga gcccggggac ccagctttct tgtacaaagt
     5221 ggttgataac agcgggttaa ttaacatctt ttacccatac gatgttcctg actatgcggg
     5281 ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc cagattacgc
     5341 tgctcagtgc agcgcttaga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag
     5401 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     5461 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     5521 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     5581 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattagcttc
     5641 atcaacgcaa gacatgcgca cgaccgtctg acaggagagg aatttccgac gagcacagaa
     5701 aggacttgct cttggacgta ggcctatttc tcaggcacat gtatcaagtg ttcggacgtg
     5761 ggttttcgat ggtgtatcag ccgccgccaa ctgggagatg aggaggcttt cttggggggc
     5821 agtcagcagt tcatttcaca agacagagga acttgtaagg agatgcactg atttatcttg
     5881 gcgcaaacca gcaggacgaa ttagtgggaa tagcccgcga atatctaagt tatgcctgtc
     5941 ggcatgagca gaaacttcca attcgaaaca gtttggagag gttgtttttg ggcatacctt
     6001 ttgttagtca gcctctcgat tgctcatcgt cattacacag taccgaagtt tgatcgatct
     6061 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     6121 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     6181 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     6241 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     6301 gaacgatctg cttcgacgca ctccttcttt actccaccat ctcgtcctta ttgaaaacgt
     6361 gggtagcacc aaaacgaatc aagtcgctgg aactgaagtt accaatcacg ctggatgatt
     6421 tgccagttgg attaatcttg cctttccccg catgaataat attgatgaat gcatgcgtga
     6481 ggggtatttc gattttggca atagctgcaa ttgccgcgac atcctccaac gagcataatt
     6541 cttcagaaaa atagcgatgt tccatgttgt cagggcatgc atgatgcacg ttatgaggtg
     6601 acggtgctag gcagtattcc ctcaaagttt catagtcagt atcatattca tcattgcatt
     6661 cctgcaagag agaattgaga cgcaatccac acgctgcggc aaccttccgg cgttcgtggt
     6721 ctatttgctc ttggacgttg caaacgtaag tgttggatcc cggtcggcat ctactctatt
     6781 cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac
     6841 agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg
     6901 ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc
     6961 cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc
     7021 gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac
     7081 aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca
     7141 tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg
     7201 agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca
     7261 gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt
     7321 gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga
     7381 ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg
     7441 catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt
     7501 gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaattccc
     7561 caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac
     7621 gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta
     7681 catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc
     7741 tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca
     7801 tatcggggtc gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga
     7861 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
     7921 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     7981 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     8041 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
     8101 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     8161 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     8221 caccatgttg acggatctct aggacgcgtc gacgcgtcga cgcgtcgacg cgtcgacgcg
     8281 tcctagaagc taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8341 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8401 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgcc cgctcctttc
     8461 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8521 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8581 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8641 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8701 atctcgggct attcttttga tttataaggg attttgccga tttcggaacc accatcaaac
     8761 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     8821 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccccag
     8881 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     8941 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     9001 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     9061 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     9121 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     9181 ctgcctgtga tcaaatatca tctccctcgc agagatccga attatcagcc ttcttattca
     9241 tttctcgctt aaccgtgaca ggctgtcgat cttgagaact atgccgacat aataggaaat
     9301 cgctggataa agccgctgag gaagctgagt ggcgctattt ctttagaagt gaacgttgac
     9361 gatatcaact cccctatcca ttgctcaccg aatggtacag gtcggggacc cgaagttccg
     9421 actgtcggcc tgatgcatcc ccggctgatc gaccccagat ctggggctga gaaagcccag
     9481 taaggaaaca actgtaggtt cgagtcgcga gatcccccgg aaccaaagga agtaggttaa
     9541 acccgctccg atcaggccga gccacgccag gccgagaaca ttggttcctg taggcatcgg
     9601 gattggcgga tcaaacacta aagctactgg aacgagcaga agtcctccgg ccgccagttg
     9661 ccaggcggta aaggtgagca gaggcacggg aggttgccac ttgcgggtca gcacggttcc
     9721 gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc
     9781 gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg caaatagccc ccaggaccgc
     9841 catcaatcgt atcgggctac ctagcagagc ggcagagatg aacacgacca tcagcggctg
     9901 cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa taaacaacaa
     9961 gctccagaat agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc accagattcc
    10021 cgttggaatc tgtcggacga tcatcacgag caataaaccc gccggcaacg cccgcagcag
    10081 cataccggcg acccctcggc ctcgctgttc gggctccacg aaaacgccgg acagatgcgc
    10141 cttgtgagcg tccttggggc cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc
    10201 gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg
    10261 ctttttctcc tcgtgctcgt aaacggaccc gaacatctct ggagctttct tcagggccga
    10321 caatcggatc tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt
    10381 aatcacaatt gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt
    10441 cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc
    10501 agtaaagcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg
    10561 caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct
    10621 tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac
    10681 atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg
    10741 aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag
    10801 tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt
    10861 ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca
    10921 acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc
    10981 ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg
    11041 aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc
    11101 agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg
    11161 acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg
    11221 aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca
    11281 atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg
    11341 gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg
    11401 gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt
    11461 tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt
    11521 tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg
    11581 aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg
    11641 gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt
    11701 caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt
    11761 gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg
    11821 tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc
    11881 agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa acacttgatg
    11941 cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta
    12001 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
    12061 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
    12121 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
    12181 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
    12241 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
    12301 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
    12361 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
    12421 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
    12481 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
    12541 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
    12601 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
    12661 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
    12721 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
    12781 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
    12841 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
    12901 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
    12961 ccgattttga aaccacaatt atgggtgatg ctgccaactt actgatttag tgtatgatgg
    13021 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact
    13081 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct ctgctctcac
    13141 tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg tacgcaccag
    13201 aaaatcattg atatggccat gaatggcgtt ggatgccggg caacagcccg cattatgggc
    13261 gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg gtaacctcgc
    13321 gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag tggggctatg
    13381 tcggggctaa atcgcgccag cgctggctgt tttacgcgta tgacagtctc cggaagacgg
    13441 ttgttgcgca cgtattcggt gaacgcacta tggcgacgct ggggcgtctt atgagcctgc
    13501 tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat gaatcccgcc
    13561 tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag cggcataacc
    13621 tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg
    13681 tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat caataagttg
    13741 gagtcattac ccaattatga tagaatttac aagctataag gttattgtcc tgggtttcaa
    13801 gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg ataagcgcgc
    13861 tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata taaaagatat
    13921 attatcttat cagtattgtc aatatattca aggcaatctg cctcctcatc ctcttcatcc
    13981 tcttcgtctt ggtagctttt taaatatggc gcttcataga gtaattctgt aaaggtccaa
    14041 ttctcgtttt catacctcgg tataatctta cctatcacct caaatggttc gctgggttta
    14101 tcgcaccccc gaacacgagc acggcacccg cgaccactat gccaagaatg cccaaggtaa
    14161 aaattgccgg ccccgccatg aagtccgtga atgccccgac ggccgaagtg aagggcaggc
    14221 cgccacccag gccgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca
    14281 cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg
    14341 ccccgatccc ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg
    14401 cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt
    14461 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg
    14521 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg
    14581 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca
    14641 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc
    14701 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc
    14761 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc
    14821 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc
    14881 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc
    14941 aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac
    15001 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc
    15061 agcccggtga gcgtcggaaa ggcgc
//
