LOCUS       sequence-247099-       15502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15502)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX107
FEATURES             Location/Qualifiers
     source          1..15502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5616..5640)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5669..5758
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5792..6044
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6473..6725)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7193..8218)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7193..8218)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8228..8572)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8714..8731)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8714..8730)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8723..8745)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8870..8889)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9080..9101
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9348..9372
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10968..12116)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12415..13209)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13401..14168
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14861..15492
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgactcc
     4621 accgccgata gagcggaacg gcggagaatc aactgtcggt gaaacaacga ggtctgtccc
     4681 gacgccgttt ttaacaaaga cgtaccagct cgtcgaggat ccttccatcg acgacgtagt
     4741 ctcttggaac gaagacggat ctacctttat cgtatggaat ccgacggagt ttgctagaga
     4801 tttgcttcct aaatatttta agcataacaa tttctctagc ttcgttcggc agctcaacac
     4861 ctatggattt cggaaagtcg tacctgatcg atgggaattc tcaaacgact gctttcggag
     4921 aggtgacaaa agtctgttac gcgatatcca gcgtcggaaa gtagtcacgc cgattactac
     4981 tccttgcgca gctgtaacag cggtcgtgac cgtggctgct ccgcctgctc agccaccacg
     5041 gcctgtaata tctacctcgg attctggtga cgagcaagtc gtctcttcaa attcatctac
     5101 aggtggcaca gcggggctgc tgggagaaaa cgaacggtta aggaaagaga atttacagct
     5161 caacaaagag ctgagccaaa tgaggagcct ctgcggcaat atatactctt tgatgtcgaa
     5221 ttacgcagac ccttcgacca gtggtaacca gtcggcggag agtatttcac cggcggcaaa
     5281 gccacttgat ctcttaccga caaaacggtc tgttgatgaa tcagatatca ataaggccat
     5341 gggcgatggg aaccgggctg aacccgagga tattcaggcg agattattcg gtttctctat
     5401 aggtatgaaa cgtgcgaggg aaggcgatga agcaagggcg gagcatggcc aggaattacg
     5461 cctgcggcag cctgggaacg acgtgaaatc agaaccgttg gatcaggaaa atggcggtga
     5521 aaatgaagag agatcgtggc tgatacattg cagtggacga aatcaaaggg tgtgtaatga
     5581 tagtgagggt ggtgttagta acttagatcc cggggaccca gctttcttgt acaaagtggt
     5641 tgataacagc gggttaatta acatctttta cccatacgat gttcctgact atgcgggcta
     5701 tccctatgac gtcccggact atgcaggatc ctatccatat gacgttccag attacgctgc
     5761 tcagtgcagc gcttagagct cgaatttccc cgatcgttca aacatttggc aataaagttt
     5821 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     5881 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     5941 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     6001 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tagcttcatc
     6061 aacgcaagac atgcgcacga ccgtctgaca ggagaggaat ttccgacgag cacagaaagg
     6121 acttgctctt ggacgtaggc ctatttctca ggcacatgta tcaagtgttc ggacgtgggt
     6181 tttcgatggt gtatcagccg ccgccaactg ggagatgagg aggctttctt ggggggcagt
     6241 cagcagttca tttcacaaga cagaggaact tgtaaggaga tgcactgatt tatcttggcg
     6301 caaaccagca ggacgaatta gtgggaatag cccgcgaata tctaagttat gcctgtcggc
     6361 atgagcagaa acttccaatt cgaaacagtt tggagaggtt gtttttgggc ataccttttg
     6421 ttagtcagcc tctcgattgc tcatcgtcat tacacagtac cgaagtttga tcgatctagt
     6481 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
     6541 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
     6601 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
     6661 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
     6721 cgatctgctt cgacgcactc cttctttact ccaccatctc gtccttattg aaaacgtggg
     6781 tagcaccaaa acgaatcaag tcgctggaac tgaagttacc aatcacgctg gatgatttgc
     6841 cagttggatt aatcttgcct ttccccgcat gaataatatt gatgaatgca tgcgtgaggg
     6901 gtatttcgat tttggcaata gctgcaattg ccgcgacatc ctccaacgag cataattctt
     6961 cagaaaaata gcgatgttcc atgttgtcag ggcatgcatg atgcacgtta tgaggtgacg
     7021 gtgctaggca gtattccctc aaagtttcat agtcagtatc atattcatca ttgcattcct
     7081 gcaagagaga attgagacgc aatccacacg ctgcggcaac cttccggcgt tcgtggtcta
     7141 tttgctcttg gacgttgcaa acgtaagtgt tggatcccgg tcggcatcta ctctattcct
     7201 ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc
     7261 catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc
     7321 cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt
     7381 caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg
     7441 gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag
     7501 ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg
     7561 cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc
     7621 cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct
     7681 catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat
     7741 acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc
     7801 cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat
     7861 ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca
     7921 acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aattccccaa
     7981 tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat
     8041 ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat
     8101 cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt
     8161 cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat
     8221 cggggtcgtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
     8281 atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt
     8341 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
     8401 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
     8461 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
     8521 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
     8581 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
     8641 catgttgacg gatctctagg acgcgtcgac gcgtcgacgc gtcgacgcgt cgacgcgtcc
     8701 tagaagctaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     8761 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     8821 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgcccgc tcctttcgct
     8881 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     8941 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     9001 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9061 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9121 tcgggctatt cttttgattt ataagggatt ttgccgattt cggaaccacc atcaaacagg
     9181 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     9241 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accccagtac
     9301 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     9361 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     9421 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     9481 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     9541 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     9601 cctgtgatca aatatcatct ccctcgcaga gatccgaatt atcagccttc ttattcattt
     9661 ctcgcttaac cgtgacaggc tgtcgatctt gagaactatg ccgacataat aggaaatcgc
     9721 tggataaagc cgctgaggaa gctgagtggc gctatttctt tagaagtgaa cgttgacgat
     9781 atcaactccc ctatccattg ctcaccgaat ggtacaggtc ggggacccga agttccgact
     9841 gtcggcctga tgcatccccg gctgatcgac cccagatctg gggctgagaa agcccagtaa
     9901 ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt aggttaaacc
     9961 cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag gcatcgggat
    10021 tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg ccagttgcca
    10081 ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca cggttccgaa
    10141 cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt
    10201 tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca ggaccgccat
    10261 caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca gcggctgcac
    10321 agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa acaacaagct
    10381 ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc agattcccgt
    10441 tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc gcagcagcat
    10501 accggcgacc cctcggcctc gctgttcggg ctccacgaaa acgccggaca gatgcgcctt
    10561 gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac agcccaatga tctcgccgtc
    10621 gatgtaggcg ccgaatgcca cggcatctcg caaccgttca gcgaacgcct ccatgggctt
    10681 tttctcctcg tgctcgtaaa cggacccgaa catctctgga gctttcttca gggccgacaa
    10741 tcggatctcg cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct gagccttaat
    10801 cacaattgtc aattttaatc ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc
    10861 gagcgatact gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt
    10921 aaagcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa
    10981 tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg
    11041 caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg
    11101 aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac
    11161 atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg
    11221 tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc
    11281 ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg
    11341 cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc
    11401 gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag
    11461 gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt
    11521 tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg
    11581 tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac
    11641 agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata
    11701 tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc
    11761 tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc
    11821 gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg
    11881 agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc
    11941 acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag
    12001 gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa
    12061 aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac
    12121 cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac
    12181 ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag
    12241 accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc
    12301 gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg
    12361 aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta ggtactaaaa
    12421 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
    12481 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
    12541 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
    12601 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
    12661 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
    12721 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
    12781 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
    12841 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
    12901 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
    12961 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
    13021 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
    13081 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
    13141 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
    13201 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
    13261 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
    13321 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
    13381 attttgaaac cacaattatg ggtgatgctg ccaacttact gatttagtgt atgatggtgt
    13441 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
    13501 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
    13561 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
    13621 atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt
    13681 ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgca
    13741 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg
    13801 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga cagtctccgg aagacggttg
    13861 ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg agcctgctgt
    13921 caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa tcccgcctga
    13981 agggaaagct gcacgtaatc agcaagcgat atacgcagcg aattgagcgg cataacctga
    14041 atctgaggca gcacctggca cggctgggac ggaagtcgct gtcgttctca aaatcggtgg
    14101 agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcaa taagttggag
    14161 tcattaccca attatgatag aatttacaag ctataaggtt attgtcctgg gtttcaagca
    14221 ttagtccatg caagttttta tgctttgccc attctataga tatattgata agcgcgctgc
    14281 ctatgccttg ccccctgaaa tccttacata cggcgatatc ttctatataa aagatatatt
    14341 atcttatcag tattgtcaat atattcaagg caatctgcct cctcatcctc ttcatcctct
    14401 tcgtcttggt agctttttaa atatggcgct tcatagagta attctgtaaa ggtccaattc
    14461 tcgttttcat acctcggtat aatcttacct atcacctcaa atggttcgct gggtttatcg
    14521 cacccccgaa cacgagcacg gcacccgcga ccactatgcc aagaatgccc aaggtaaaaa
    14581 ttgccggccc cgccatgaag tccgtgaatg ccccgacggc cgaagtgaag ggcaggccgc
    14641 cacccaggcc gccgccctca ctgcccggca cctggtcgct gaatgtcgat gccagcacct
    14701 gcggcacgtc aatgcttccg ggcgtcgcgc tcgggctgat cgcccatccc gttactgccc
    14761 cgatcccggc aatggcaagg actgccagcg ctgccatttt tggggtgagg ccgttcgcgg
    14821 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
    14881 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
    14941 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
    15001 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
    15061 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
    15121 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
    15181 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
    15241 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
    15301 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
    15361 ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc
    15421 gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc
    15481 ccggtgagcg tcggaaaggc gc
//
