LOCUS       sequence-247101-       15505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15505)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX108
FEATURES             Location/Qualifiers
     source          1..15505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5619..5643)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5672..5761
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5795..6047
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6476..6728)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7196..8221)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7196..8221)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8231..8575)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8717..8734)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8717..8733)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8726..8748)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8873..8892)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9083..9104
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9351..9375
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10971..12119)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12418..13212)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13404..14171
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14864..15495
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg gatcatttcg gttcaataaa
     4621 ggaagagttt actttcatgt ccaatatgcc tcagccaatg gagggtcttc atgagagtgg
     4681 ccctccacca tttctcaaca aaacttacca acttgtcgat gatccaagca ccaacgatgt
     4741 cgtttcttgg agtagaggta acaatagttt catcgtttgg gatcctcaaa ccttggccat
     4801 gaatcttctt ccaaggtact tcaagcataa caacttctcc agctttgtca gacagctcaa
     4861 tacttatgga tttaggaagg ttaatccaga ccattgggag tttgctaatg aaggtttctt
     4921 gagggggaac aagcatctct tgaagaaagt taggaggaga aagacaagtt ataataattg
     4981 tttacactca gctggcactt ccaataaggc gtcaaaccaa ggtatggagt actcttcatg
     5041 tgttgagttg gggaagtttg gattagatgg agaaattgat cgactgaggc gcgacaagca
     5101 ggttctaatg atggaacttg ttaatcttag acagcagcag caggctaata aatcatacct
     5161 gaaatcaatt gaagaaaaac ttaaagggac agaaatgaag cagcagcaga tgatgagttt
     5221 cttggccaaa gcaatacaga atccaaactt tgtgcagcag ataatgcagc agaaggataa
     5281 aaggaaacaa cttgaggaag aaatcaacaa gaaaaggagg cggccaattg accaaggtcc
     5341 tatcaatata gaaaatgaat ttaatataaa gcaagaatct gcccaagatt attgtgatgt
     5401 ttatggattt gatgatttag aactggagga agatgactat gttgatcaga aagcacatat
     5461 agaaagtgga gataaagcaa ttaatgaagg attttgggag gacttgttga atgaaaatat
     5521 tggtgaatat gagatggctt tacttggtat tgaagaagag gatatggatt ctttgtctga
     5581 acaccttgac tttttaggtt ctaacctaaa acccggggac ccagctttct tgtacaaagt
     5641 ggttgataac agcgggttaa ttaacatctt ttacccatac gatgttcctg actatgcggg
     5701 ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc cagattacgc
     5761 tgctcagtgc agcgcttaga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag
     5821 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     5881 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     5941 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     6001 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattagcttc
     6061 atcaacgcaa gacatgcgca cgaccgtctg acaggagagg aatttccgac gagcacagaa
     6121 aggacttgct cttggacgta ggcctatttc tcaggcacat gtatcaagtg ttcggacgtg
     6181 ggttttcgat ggtgtatcag ccgccgccaa ctgggagatg aggaggcttt cttggggggc
     6241 agtcagcagt tcatttcaca agacagagga acttgtaagg agatgcactg atttatcttg
     6301 gcgcaaacca gcaggacgaa ttagtgggaa tagcccgcga atatctaagt tatgcctgtc
     6361 ggcatgagca gaaacttcca attcgaaaca gtttggagag gttgtttttg ggcatacctt
     6421 ttgttagtca gcctctcgat tgctcatcgt cattacacag taccgaagtt tgatcgatct
     6481 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     6541 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     6601 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     6661 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     6721 gaacgatctg cttcgacgca ctccttcttt actccaccat ctcgtcctta ttgaaaacgt
     6781 gggtagcacc aaaacgaatc aagtcgctgg aactgaagtt accaatcacg ctggatgatt
     6841 tgccagttgg attaatcttg cctttccccg catgaataat attgatgaat gcatgcgtga
     6901 ggggtatttc gattttggca atagctgcaa ttgccgcgac atcctccaac gagcataatt
     6961 cttcagaaaa atagcgatgt tccatgttgt cagggcatgc atgatgcacg ttatgaggtg
     7021 acggtgctag gcagtattcc ctcaaagttt catagtcagt atcatattca tcattgcatt
     7081 cctgcaagag agaattgaga cgcaatccac acgctgcggc aaccttccgg cgttcgtggt
     7141 ctatttgctc ttggacgttg caaacgtaag tgttggatcc cggtcggcat ctactctatt
     7201 cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac
     7261 agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg
     7321 ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc
     7381 cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc
     7441 gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac
     7501 aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca
     7561 tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg
     7621 agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca
     7681 gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt
     7741 gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga
     7801 ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg
     7861 catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt
     7921 gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaattccc
     7981 caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac
     8041 gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta
     8101 catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc
     8161 tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca
     8221 tatcggggtc gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga
     8281 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
     8341 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     8401 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     8461 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
     8521 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     8581 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     8641 caccatgttg acggatctct aggacgcgtc gacgcgtcga cgcgtcgacg cgtcgacgcg
     8701 tcctagaagc taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8761 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8821 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgcc cgctcctttc
     8881 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8941 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9001 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9061 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9121 atctcgggct attcttttga tttataaggg attttgccga tttcggaacc accatcaaac
     9181 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     9241 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccccag
     9301 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     9361 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     9421 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     9481 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     9541 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     9601 ctgcctgtga tcaaatatca tctccctcgc agagatccga attatcagcc ttcttattca
     9661 tttctcgctt aaccgtgaca ggctgtcgat cttgagaact atgccgacat aataggaaat
     9721 cgctggataa agccgctgag gaagctgagt ggcgctattt ctttagaagt gaacgttgac
     9781 gatatcaact cccctatcca ttgctcaccg aatggtacag gtcggggacc cgaagttccg
     9841 actgtcggcc tgatgcatcc ccggctgatc gaccccagat ctggggctga gaaagcccag
     9901 taaggaaaca actgtaggtt cgagtcgcga gatcccccgg aaccaaagga agtaggttaa
     9961 acccgctccg atcaggccga gccacgccag gccgagaaca ttggttcctg taggcatcgg
    10021 gattggcgga tcaaacacta aagctactgg aacgagcaga agtcctccgg ccgccagttg
    10081 ccaggcggta aaggtgagca gaggcacggg aggttgccac ttgcgggtca gcacggttcc
    10141 gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc
    10201 gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg caaatagccc ccaggaccgc
    10261 catcaatcgt atcgggctac ctagcagagc ggcagagatg aacacgacca tcagcggctg
    10321 cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa taaacaacaa
    10381 gctccagaat agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc accagattcc
    10441 cgttggaatc tgtcggacga tcatcacgag caataaaccc gccggcaacg cccgcagcag
    10501 cataccggcg acccctcggc ctcgctgttc gggctccacg aaaacgccgg acagatgcgc
    10561 cttgtgagcg tccttggggc cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc
    10621 gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg
    10681 ctttttctcc tcgtgctcgt aaacggaccc gaacatctct ggagctttct tcagggccga
    10741 caatcggatc tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt
    10801 aatcacaatt gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt
    10861 cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc
    10921 agtaaagcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg
    10981 caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct
    11041 tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac
    11101 atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg
    11161 aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag
    11221 tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt
    11281 ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca
    11341 acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc
    11401 ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg
    11461 aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc
    11521 agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg
    11581 acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg
    11641 aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca
    11701 atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg
    11761 gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg
    11821 gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt
    11881 tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt
    11941 tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg
    12001 aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg
    12061 gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt
    12121 caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt
    12181 gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg
    12241 tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc
    12301 agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa acacttgatg
    12361 cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta
    12421 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
    12481 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
    12541 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
    12601 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
    12661 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
    12721 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
    12781 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
    12841 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
    12901 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
    12961 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
    13021 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
    13081 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
    13141 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
    13201 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
    13261 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
    13321 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
    13381 ccgattttga aaccacaatt atgggtgatg ctgccaactt actgatttag tgtatgatgg
    13441 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact
    13501 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct ctgctctcac
    13561 tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg tacgcaccag
    13621 aaaatcattg atatggccat gaatggcgtt ggatgccggg caacagcccg cattatgggc
    13681 gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg gtaacctcgc
    13741 gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag tggggctatg
    13801 tcggggctaa atcgcgccag cgctggctgt tttacgcgta tgacagtctc cggaagacgg
    13861 ttgttgcgca cgtattcggt gaacgcacta tggcgacgct ggggcgtctt atgagcctgc
    13921 tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat gaatcccgcc
    13981 tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag cggcataacc
    14041 tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg
    14101 tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat caataagttg
    14161 gagtcattac ccaattatga tagaatttac aagctataag gttattgtcc tgggtttcaa
    14221 gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg ataagcgcgc
    14281 tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata taaaagatat
    14341 attatcttat cagtattgtc aatatattca aggcaatctg cctcctcatc ctcttcatcc
    14401 tcttcgtctt ggtagctttt taaatatggc gcttcataga gtaattctgt aaaggtccaa
    14461 ttctcgtttt catacctcgg tataatctta cctatcacct caaatggttc gctgggttta
    14521 tcgcaccccc gaacacgagc acggcacccg cgaccactat gccaagaatg cccaaggtaa
    14581 aaattgccgg ccccgccatg aagtccgtga atgccccgac ggccgaagtg aagggcaggc
    14641 cgccacccag gccgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca
    14701 cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg
    14761 ccccgatccc ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg
    14821 cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt
    14881 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg
    14941 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg
    15001 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca
    15061 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc
    15121 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc
    15181 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc
    15241 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc
    15301 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc
    15361 aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac
    15421 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc
    15481 agcccggtga gcgtcggaaa ggcgc
//
