LOCUS       sequence-247108-       16439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16439)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX111
FEATURES             Location/Qualifiers
     source          1..16439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6516..6525
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6553..6577)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6606..6695
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6729..6981
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7410..7662)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8130..9155)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8130..9155)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9165..9509)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9651..9668)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9651..9667)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9660..9682)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9807..9826)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10017..10038
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10285..10309
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11905..13053)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13352..14146)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14338..15105
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15798..16429
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggcttcccat gggaagagga agggtacagt tgaagagaat tgagaacaaa
     5881 attaatagac aagttacttt ctcaaaacgt cgatctggtt tgcttaagaa agctcatgag
     5941 atttctgtgc tttgtgatgc tgaggttggt ttgattgttt tttctactaa aggcaaactc
     6001 tttgaatatg ccactgattc ttgcatggag aggatcctcg aaagatatga aagatactca
     6061 tatgctgaga ggcagcttgt tgctaatact gatcatagct ccccgggaag ctggactgtg
     6121 gaacatgcaa aacttaaggc tagacttgag gttatacaga gaaaccaaag gcattatatg
     6181 ggagaagatt tgaactcgtt aagcatgaaa gaacttcaga atttggagca tcagctggat
     6241 tctgctctta aacacactcg ctcaagaaag aaccaattga tgcatgagtc tatttctgag
     6301 cttcaaaaaa aggacaaagc actgcaggag caaaacaacc agctttccaa gaaggtgaag
     6361 gagaggaaga aagagctggc ccaacaaaat cagtgggagc agcagaaaga actcaactct
     6421 cctaaccttg gggaagcata tcagagtact gtaaaagaaa atggagaagt agaaggaggt
     6481 tcgaattcga agcagcaaac tgctaatact gtgatgccac catggatgat tcgccatctt
     6541 aatggctcga ggacccagct ttcttgtaca aagtggttga taacagcggg ttaattaaca
     6601 tcttttaccc atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg
     6661 caggatccta tccatatgac gttccagatt acgctgctca gtgcagcgct tagagctcga
     6721 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     6781 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     6841 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     6901 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     6961 tgtcatctat gttactagat cgggaattag cttcatcaac gcaagacatg cgcacgaccg
     7021 tctgacagga gaggaatttc cgacgagcac agaaaggact tgctcttgga cgtaggccta
     7081 tttctcaggc acatgtatca agtgttcgga cgtgggtttt cgatggtgta tcagccgccg
     7141 ccaactggga gatgaggagg ctttcttggg gggcagtcag cagttcattt cacaagacag
     7201 aggaacttgt aaggagatgc actgatttat cttggcgcaa accagcagga cgaattagtg
     7261 ggaatagccc gcgaatatct aagttatgcc tgtcggcatg agcagaaact tccaattcga
     7321 aacagtttgg agaggttgtt tttgggcata ccttttgtta gtcagcctct cgattgctca
     7381 tcgtcattac acagtaccga agtttgatcg atctagtaac atagatgaca ccgcgcgcga
     7441 taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg
     7501 cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat
     7561 tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg
     7621 attcaatctt aagaaacttt attgccaaat gtttgaacga tctgcttcga cgcactcctt
     7681 ctttactcca ccatctcgtc cttattgaaa acgtgggtag caccaaaacg aatcaagtcg
     7741 ctggaactga agttaccaat cacgctggat gatttgccag ttggattaat cttgcctttc
     7801 cccgcatgaa taatattgat gaatgcatgc gtgaggggta tttcgatttt ggcaatagct
     7861 gcaattgccg cgacatcctc caacgagcat aattcttcag aaaaatagcg atgttccatg
     7921 ttgtcagggc atgcatgatg cacgttatga ggtgacggtg ctaggcagta ttccctcaaa
     7981 gtttcatagt cagtatcata ttcatcattg cattcctgca agagagaatt gagacgcaat
     8041 ccacacgctg cggcaacctt ccggcgttcg tggtctattt gctcttggac gttgcaaacg
     8101 taagtgttgg atcccggtcg gcatctactc tattcctttg ccctcggacg agtgctgggg
     8161 cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
     8221 ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
     8281 cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
     8341 tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc
     8401 ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
     8461 atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
     8521 gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
     8581 cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
     8641 gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
     8701 catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
     8761 ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc
     8821 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg
     8881 gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg
     8941 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
     9001 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
     9061 tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
     9121 tcgacagacg tcgcggtgag ttcaggcttt ttcatatcgg ggtcgtcctc tccaaatgaa
     9181 atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt gcgtcatccc
     9241 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
     9301 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
     9361 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
     9421 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
     9481 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
     9541 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttgacggat ctctaggacg
     9601 cgtcgacgcg tcgacgcgtc gacgcgtcga cgcgtcctag aagctaattc actggccgtc
     9661 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     9721 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9781 cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     9841 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     9901 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     9961 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    10021 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
    10081 agggattttg ccgatttcgg aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
    10141 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg
    10201 cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt aaaaacgtcc gcaatgtgtt
    10261 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
    10321 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
    10381 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
    10441 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
    10501 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaaat atcatctccc
    10561 tcgcagagat ccgaattatc agccttctta ttcatttctc gcttaaccgt gacaggctgt
    10621 cgatcttgag aactatgccg acataatagg aaatcgctgg ataaagccgc tgaggaagct
    10681 gagtggcgct atttctttag aagtgaacgt tgacgatatc aactccccta tccattgctc
    10741 accgaatggt acaggtcggg gacccgaagt tccgactgtc ggcctgatgc atccccggct
    10801 gatcgacccc agatctgggg ctgagaaagc ccagtaagga aacaactgta ggttcgagtc
    10861 gcgagatccc ccggaaccaa aggaagtagg ttaaacccgc tccgatcagg ccgagccacg
    10921 ccaggccgag aacattggtt cctgtaggca tcgggattgg cggatcaaac actaaagcta
    10981 ctggaacgag cagaagtcct ccggccgcca gttgccaggc ggtaaaggtg agcagaggca
    11041 cgggaggttg ccacttgcgg gtcagcacgg ttccgaacgc catggaaacc gcccccgcca
    11101 ggcccgctgc gacgccgaca ggatctagcg ctgcgtttgg tgtcaacacc aacagcgcca
    11161 cgcccgcagt tccgcaaata gcccccagga ccgccatcaa tcgtatcggg ctacctagca
    11221 gagcggcaga gatgaacacg accatcagcg gctgcacagc gcctaccgtc gccgcgaccc
    11281 cgcccggcag gcggtagacc gaaataaaca acaagctcca gaatagcgaa atattaagtg
    11341 cgccgaggat gaagatgcgc atccaccaga ttcccgttgg aatctgtcgg acgatcatca
    11401 cgagcaataa acccgccggc aacgcccgca gcagcatacc ggcgacccct cggcctcgct
    11461 gttcgggctc cacgaaaacg ccggacagat gcgccttgtg agcgtccttg gggccgtcct
    11521 cctgtttgaa gaccgacagc ccaatgatct cgccgtcgat gtaggcgccg aatgccacgg
    11581 catctcgcaa ccgttcagcg aacgcctcca tgggcttttt ctcctcgtgc tcgtaaacgg
    11641 acccgaacat ctctggagct ttcttcaggg ccgacaatcg gatctcgcgg aaatcctgca
    11701 cgtcggccgc tccaagccgt cgaatctgag ccttaatcac aattgtcaat tttaatcctc
    11761 tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt
    11821 gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc
    11881 agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat cattgaccca
    11941 ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg
    12001 ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt ccagcttgag
    12061 cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg tcggcgacag
    12121 cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat
    12181 ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca ggacgcggaa
    12241 gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga agcccatcgc
    12301 cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc cattgatcga
    12361 ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt
    12421 gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg cccgcagctc
    12481 gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag
    12541 cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt
    12601 tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc
    12661 cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt
    12721 tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat
    12781 ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc
    12841 ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct cgatcttggc
    12901 cgtagcttgc tggaccatcg agccgacgga ctggaaggtt tcgcggggcg cacgcatgac
    12961 ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg
    13021 cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga ttgccccgac
    13081 tcacgccggg gcaatgtgcc cttattcctg atttgacccg cctggtgcct tggtgtccag
    13141 ataatccacc ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt ccttctcgta
    13201 cttggtattc cgaatcttgc cctgcacgaa taccagcgac cccttgccca aatacttgcc
    13261 gtgggcctcg gcctgagagc caaaacactt gatgcggaag aagtcggtgc gctcctgctt
    13321 gtcgccggca tcgttgcgcc acatctaggt actaaaacaa ttcatccagt aaaatataat
    13381 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
    13441 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
    13501 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
    13561 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
    13621 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
    13681 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
    13741 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
    13801 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
    13861 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
    13921 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
    13981 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
    14041 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
    14101 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
    14161 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
    14221 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
    14281 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccac aattatgggt
    14341 gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc
    14401 tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag
    14461 caccgccgga catcagcgct atctctgctc tcactgccgt aaaacatggc aactgcagtt
    14521 cacttacacc gcttctcaac ccggtacgca ccagaaaatc attgatatgg ccatgaatgg
    14581 cgttggatgc cgggcaacag cccgcattat gggcgttggc ctcaacacga ttttacgtca
    14641 cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc
    14701 gtctgcgcgg aaatggacga acagtggggc tatgtcgggg ctaaatcgcg ccagcgctgg
    14761 ctgttttacg cgtatgacag tctccggaag acggttgttg cgcacgtatt cggtgaacgc
    14821 actatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt ggtgatatgg
    14881 atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca cgtaatcagc
    14941 aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca cctggcacgg
    15001 ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg
    15061 cattatctga acataaaaca ctatcaataa gttggagtca ttacccaatt atgatagaat
    15121 ttacaagcta taaggttatt gtcctgggtt tcaagcatta gtccatgcaa gtttttatgc
    15181 tttgcccatt ctatagatat attgataagc gcgctgccta tgccttgccc cctgaaatcc
    15241 ttacatacgg cgatatcttc tatataaaag atatattatc ttatcagtat tgtcaatata
    15301 ttcaaggcaa tctgcctcct catcctcttc atcctcttcg tcttggtagc tttttaaata
    15361 tggcgcttca tagagtaatt ctgtaaaggt ccaattctcg ttttcatacc tcggtataat
    15421 cttacctatc acctcaaatg gttcgctggg tttatcgcac ccccgaacac gagcacggca
    15481 cccgcgacca ctatgccaag aatgcccaag gtaaaaattg ccggccccgc catgaagtcc
    15541 gtgaatgccc cgacggccga agtgaagggc aggccgccac ccaggccgcc gccctcactg
    15601 cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg gcacgtcaat gcttccgggc
    15661 gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga tcccggcaat ggcaaggact
    15721 gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag cccctggggg
    15781 gatgggaggc ccgcgttagc gggccgggag ggttcgagaa gggggggcac cccccttcgg
    15841 cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta taaatattgg
    15901 tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga aacccttgca
    15961 aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg cccctcatct
    16021 gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc
    16081 cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca tctgtgggaa
    16141 actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg
    16201 ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt
    16261 gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt atccacaacg
    16321 ccggcggccg cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc
    16381 atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtcg gaaaggcgc
//
