LOCUS       sequence-24711-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Vorvis C, Markus SM, Lee WL
  TITLE     Photoactivatable GFP tagging cassettes for protein-tracking studies 
            in the budding yeast Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2008 Sep;25(9):651-9.
  PUBMED    18727145
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2008-09"; volume: "25(9)"; pages: "651-9"
COMMENT     Sequence Label: pFA6a-PA-GFP-kanMX6
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(55..57,58..60,61..771)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALXKDPNEKRDHMVLL
                     EFVTAAGXTLGMXXLYK"
     primer_bind     complement(100..121)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(361..380)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     708..729
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cttcntacgc tgcaggtcga cggnnccccg ggttaattaa ggcgcgccag atccatggtg
       61 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      121 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      181 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      241 accaccttca gctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      301 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      361 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      421 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      481 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
      541 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      601 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      661 agccaccagt ccgccctgan caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      721 gagttcgtga ccgccgccgg gntcactctc ggcatggnng anctgtacaa gtagagatct
      781 gtttagctng nctcgtcccc gccgggncac ccngnccann nacatggnan gccca
//
