LOCUS       sequence-247125-        9634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  control plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9634)
  TITLE     Root Lab control plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLX_TRC209
FEATURES             Location/Qualifiers
     source          1..9634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(234..253)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     581..602
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    672..1260
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(725..745)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1143..1154)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1332..1565
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1643..1764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1680..1699)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1734..1753
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1804..1939
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1959..1978)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1960..1978)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1988..2005)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1988..2004)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1997..2019)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2146..2601
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2233..2252)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2443..2464
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2627..2731
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2732..2800,2801..3592)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2950..2969)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3763..4351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4252..4271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4505..4522
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4639..4660
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4675..4680,4681..4698,4699..4705)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4713..4729
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4718..4740
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4737..4753
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4737..4753
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4772..4792
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4774..4792
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4820..5046
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5047..5227
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5274..5399
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5892..6125
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6310..6354
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6529..7029
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6948..6966
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7041..8060
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    8117..8234
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8373..9551
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8604..9542
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9499..9519
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(9562..9564,9565..9567,9568..9634,1..650)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKS"
     primer_bind     complement(9607..9628)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gccacaagtt cagcgtgtct ggcgagggcg agggcgatgc cacctacggc aagctgaccc
       61 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      121 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      181 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      241 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      301 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      361 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcga
      421 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      481 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      541 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      601 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtcctaa ctcgagacgc
      661 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
      721 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
      781 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
      841 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
      901 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
      961 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     1021 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     1081 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     1141 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     1201 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     1261 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     1321 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     1381 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     1441 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     1501 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     1561 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     1621 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     1681 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     1741 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     1801 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     1861 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     1921 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     1981 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     2041 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     2101 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     2161 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2221 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2281 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2341 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     2401 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2461 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     2521 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2581 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2641 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2701 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2761 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2821 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2881 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2941 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3001 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3061 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3121 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3181 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3241 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3301 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3361 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3421 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3481 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3541 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3601 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3661 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3721 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3781 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3841 ggatcaagag ccaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3901 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3961 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4021 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4081 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4141 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4201 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4261 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4321 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4381 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4441 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4501 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     4561 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     4621 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     4681 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     4741 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     4801 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     4861 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     4921 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     4981 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     5041 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5101 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5161 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5221 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     5281 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     5341 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     5401 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     5461 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     5521 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     5581 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     5641 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     5701 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     5761 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     5821 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     5881 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     5941 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     6001 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     6061 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     6121 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     6181 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     6241 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     6301 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     6361 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     6421 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     6481 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc
     6541 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     6601 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     6661 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     6721 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     6781 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     6841 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     6901 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     6961 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     7021 tctccccagg gggtaccacc atgaaaaagc ctgaactcac cgctacctct gtcgagaagt
     7081 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctccgag ggcgaagaat
     7141 ctcgggcttt cagcttcgat gtgggagggc gtggatatgt cctgcgggtg aatagctgcg
     7201 ccgatggttt ctacaaagat cgctatgttt atcggcactt tgcatccgcc gctctcccta
     7261 ttcccgaagt gcttgacatt ggggagttca gcgagagcct gacctattgc atctcccgcc
     7321 gtgcacaggg tgtcaccttg caagacctgc ctgaaaccga actgcccgct gttctccagc
     7381 ccgtcgccga ggccatggat gccatcgctg ccgccgatct tagccagacc agcgggttcg
     7441 gcccattcgg acctcaagga atcggtcaat acactacatg gcgcgatttc atctgcgcta
     7501 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcctccg
     7561 tcgcccaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     7621 tcgtgcacgc cgatttcggc tccaacaatg tcctgaccga caatggccgc ataacagccg
     7681 tcattgactg gagcgaggcc atgttcgggg attcccaata cgaggtcgcc aacatcttct
     7741 tctggaggcc ctggttggct tgtatggagc agcagacccg ctacttcgag cggaggcatc
     7801 ccgagcttgc aggatctcct cggctccggg cttatatgct ccgcattggt cttgaccaac
     7861 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggctcag ggtcgctgcg
     7921 acgcaatcgt ccggtccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     7981 ctgccgtctg gaccgatggc tgtgtggaag tgctcgccga tagtggaaac agacgcccca
     8041 gcactcgtcc tagggcaaag gatctgcagt aatgagaatt ctagatcttg agacaaatgg
     8101 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     8161 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     8221 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
     8281 agggggcccg ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
     8341 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
     8401 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     8461 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     8521 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     8581 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     8641 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
     8701 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     8761 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     8821 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     8881 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     8941 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     9001 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     9061 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     9121 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     9181 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     9241 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     9301 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     9361 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     9421 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     9481 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     9541 aggtgtcgtg agggatccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     9601 cccatcctgg tcgagctgga cggcgacgta aacg
//
