LOCUS       sequence-247126-       20648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B6J Lrig1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy 
            number.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20648)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Lrig1 expression prospectively identifies stem cells in the 
            ventricular-subventricular zone that are neurogenic throughout adult
            life.
  JOURNAL   Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5.
  PUBMED    32183906
REFERENCE   2  (bases 1 to 20648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neural 
            Dev."; date: "2020-03-17"; volume: "15(1):3. doi"; pages: " 
            10.1186/s13064-020-00139-5"
COMMENT     Sequence Label: pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK
FEATURES             Location/Qualifiers
     source          1..20648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(23..70)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(82..581)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(130..149)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    complement(645..678)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             4341..4352
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6026..6050)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6295..6357
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           6379..6418
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             6436..7566
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(7564..7583)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7608..7656
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(7960..7977)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7960..7976)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7969..7991)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8185..8204)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8304..8326
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8364..8382)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8450..8554
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8555..8623,8624..9415)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8773..8792)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9580..10125
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     10026..10045
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10242..10259
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10409..10428
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10409..10427
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    10670..10694
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             16626..16679
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(16689..16691,16692..17402)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             16689..17402
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(16719..16747)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     17342..17362
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             18525..19460
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             18573..19466
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     protein_bind    complement(19499..19532)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    19587..19811
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     19800..19817
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(19849..20648,1..4))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(19960..19979)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     20570..20589
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ccatggttta gttcctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt
       61 aattgtcaac aggctgcagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc
      121 agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc
      181 cccgcccctg agcccgcccc tgagcccgcc cccggaccca ccccttccca gcctctgagc
      241 ccagaaagcg aaggagcaaa gctgctattg gccgctgccc caaaggccta cccgcttcca
      301 ttgctcagcg gtgctgtcca tctgcacgag actagtgaga cgtgctactt ccatttgtca
      361 cgtcctgcac gacgcgagct gcggggcggg ggggaacttc ctgactaggg gaggagtaga
      421 aggtggcgcg aaggggccac caaagaacgg agccggttgg cgcctaccgg tggatgtgga
      481 atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc
      541 gcatgctcca gactgccttg ggaaaagcgc ctcccctacc cggtagaatt tcgacgacct
      601 gcagccaagc tagcttggct ggacgtaaac tcctcttcag acctgaagtt cctatacttt
      661 ctagagaata ggaacttcgg aattcgcttc atctacctca gctcttttaa agcagtcgct
      721 acaggaaaga ggtaggagag gccgtggaaa gcttgtgtcc agacgtcact ccggacagtc
      781 cgacttccgt gtggaatgtc agtcagtcgg ctagaggagt ggcatctgga gctcagaaac
      841 gtgagagact atttgtgtac aaaaggcagg aaaaagtatt tgataccact gtacataaga
      901 gttttcagag atttcatata tattatatat cttttacaga gactatttta atccttagcg
      961 catgggtccc gcttttggtg atattttcac attaggttgc tgtctaattt tggagagggt
     1021 caggaatcgt tctggtgcct taatgcacag ctggaattca ggtaaacaca tcagctgttg
     1081 ctggcggttg gaaaggaggg catcttggct gtgtggcact tagtggtcag attcgagtgc
     1141 actggccaga cctatgcagg cgtggtgttg gcagcctcac tgtagagcag ggtgcacggg
     1201 tctgtagttg aggacgagat tgagagctgt taatccacca aatgcaatgg ctcagaacaa
     1261 ttaagcactg cctttcgtct ttctttgtaa ctcacttggt aaaagcaaat gtcttgtctc
     1321 cttcagtggt tctttgaagc ttagtgaggc ggaggctgcc agtgttggta cctgtggatt
     1381 ttccaatagt gagggttagc tctgcctcca gggcccgcta agttctgctg agcaggcctg
     1441 tccacgctgt tccttaccgg tgagactggg cttggactgt acaagacttg agtcctagac
     1501 ctgggtgtat ttgcactttg gggccctgtt gctagccatc ttgtgagtgc caatgtataa
     1561 tgcagtaaag actacatgga aacacaaact cagtccttaa aaccccagga gttgagggga
     1621 agtgttggca cagtgatggc ctgtgtcacc gtgtagtgtc ataggccatc actgtgccag
     1681 agcaagcacg ctgagaaggc tagtctccat agttgatggt taatgttact tccacaaaat
     1741 atgtgaattt gctgcttctg agaggcaatg tgaaagagga agtattactt tttatgtaca
     1801 gagttattta tttatagaaa ttttggtaca gtgtacattg aaaacatgta aaatattgaa
     1861 gtgtctaaca aatggcataa agtgtcttta ataaaggttc atttataaat actaagtgtg
     1921 tgcggcggtc actgctcttt tattctgcta ttaatgtttc tccaaaagga gaatacttag
     1981 acgaagcagc atagactaca cactgttgcc ccagaaagac agtggcactg cattcctcag
     2041 atagcttttt tttttccaca tggagttggt tagtcaagcc ttatgctccc caagtatgct
     2101 gaccctccca cctcctgctg gcatatacca tgaggacaaa ggagctgctc cagctggcct
     2161 ccagggacgg acagtggtgg gcatgggctt tgcttccctc aacatgttcc taactgaagt
     2221 cacttgggcc cacccactca ctggcgctgc agctgtctgc tgtctgtcac acactccagg
     2281 gactttgggg cactttgctg taaagtaata taatcatgtg tttttatagg agaaatacaa
     2341 ggtaattaaa aacagcatcc tcaccagaaa gaatgcatgc tatgcacctg tagctcatgc
     2401 tttttaaaca aagctataat cttacacaat aggcacccgc ccaggggaga aacagccaca
     2461 cgcacggttc ttgccccctt ccttttccca gtctggtcat tcactgcagt tgtagccagt
     2521 ttgctttcct ggtgactcca acagacagca gaaagcacag gatgctaata aaaagcattc
     2581 tgaagggcct ggccttggcc ttaccgggag tatttattga gaggactgag acggtggccc
     2641 cagtttcacc aagaaaagtt tcctggggca gcctgtgctt tggccttcat taagctgctc
     2701 tctgcagcca tcttcaggga gccacactgg agccccaggc acaggaagga ctgcaaggac
     2761 atgtagtgtc tgcttcatgg gctcttcctg cccacctcta acagcaagct tcgcgcctgg
     2821 tcataggcgc ccagaaagat gaagccgccc atgctgattg ctgccatcct cgggaggaca
     2881 cctgcgaata acctagggac aggaaatggt catgttcctg actgcccgca gttcgctcat
     2941 tccatcacat ccattgtgaa atcccacaga aatttacagt gtctttcctt ccttttagaa
     3001 ggctactaag taatattccc caatatgcat gagaaactgg agcttagcct agcacggcca
     3061 cactagctgg gaacactcac attaacacag taaccactct cccagcagtg tgctcgaaga
     3121 agggagtcaa ggatacatct gaggtgaggc tggtaagggt gtgtggccct gggacatctg
     3181 aggggcctct aggtctcagt cagggagaca aagcaaattc tgaaacagtt ctgctctcct
     3241 accctgggct cggttatatt tgtcattgga gtcccacaaa tgcgattcta caagaagggg
     3301 atgatgaatc tttgtggact ccctttttct tgttcaatcg aaataaggac gccagagtca
     3361 catgactgtg gtaaaggtgg agcgcctcac agtgtcagtt cgagattctc aggtgtccca
     3421 tccccaatcc agtcagaaca ccacagtggt gtcaggaagc cattctccag aggcccagga
     3481 aatttcaaat atccatcgtg acattactga agtaatctac tagtgacctt acctggccag
     3541 atgagacagc attgtgaaaa cagagtggcg cacagctgga gagaacaaga cagaagcccc
     3601 aaccctaaaa ggttaccatc cccatggtcg ctgggctgat gaacacaggg caagtcctag
     3661 gctgtaaatt actgctttct acagaggcag tgggagtgag agagtgacag acagaaagcc
     3721 tgatcttatt tctactgaaa ccctgttacc cagaaaagtg cttctatatg cagaaagaca
     3781 aacttagaag ccagaacttg ggctgcctcg gtgggaaaag gcacttgctg tcaagactga
     3841 tggataaccc aagttcgatc cctggacaag gaaagaacca gtttgatgtc tatgtgtgca
     3901 cactcgtgga gaggctttgg gtatagggca agtgttttat tataacaatg ctcatgcctt
     3961 ttaaagatgt gggaaagcct ttgtgctaag ctaagtggaa gctaatgaag aaagctgaca
     4021 ggaatctctg ctggatacac agcccatgcc ctacacagcc agtggaagag atccgctgcc
     4081 cctcccacct ctagcaggca gcacagaaga aagcaaggac tttacatagt gggacccctc
     4141 ttgctaagct acttagggag ctagagagaa tagcttagtg ggtaaagcga gcaagacctg
     4201 agttcaaatg tctagaaacc tcgtgaggct gcgcgtggca gctctgcagt aaccccagtg
     4261 ctcctgtagt gacaggaagt cccggaggct cttgggtcag ctagcctggc ccaggaagtg
     4321 gcaaacaagg cacctaccat atagaaggca ggcctggaga cactgagctg ggcttggagg
     4381 tcagtctccc tcagctttgc cactacccag ctcagcagag gccagagaca gatgggagca
     4441 gttacagcgt cacagatgac caacagactg cagcctcctt aaagtggagg ttttgtaggg
     4501 gggaggggtt ccacctctgc ttacaggcag acttgtgagt agggcaggtt gtgttccagg
     4561 gacaacaggt gacaggataa gcaggagctc ccagcagcag cgagtacata aagggcatat
     4621 tcaagggaaa acgttcaggg gatcagccag gggcaatcca ggacaagtgg acctcccctc
     4681 tgccctgcag cttctgggct aaccccagtc tgccctccag cgtggctccc taccatctcc
     4741 tctgagatag catcaggttc cttggtggga ggggctgctg ggagttatgt agacttgata
     4801 ggcttagtgt gtgtgcaggg cagggggcac agacagtggt ggctctatct ggcactgcca
     4861 cagaggaagt aggctgagcc acctgcccag aaagggacca gtagttttca tttgtctctt
     4921 ggtccaatca cttgctcact agaggggctg gagttgctgt atgctcaggg gtggccactg
     4981 ctgctgggaa catgctgttc agtgctgccc tggacaaggt gaccagagtc caagactgga
     5041 gactcagaag acgagccaaa aatccaagct acctttcagc accaaggctg ggtcagaaca
     5101 aacacacacc cttggcctgt tttgctgtct ttcctctggc actgcttagt tatagtcaag
     5161 tgtgtgccat gacctggaca gggaaggccg ctcttgcatc atctacccag cagcatgcgg
     5221 tggccacagc ggggcactgc ctcatccagt gtgtcctttc ttgtcaccac tttctcgaca
     5281 ctggcaaatg ggctgcagcc ccacaacagt atagtcaggg gcccagctaa gtcaccacac
     5341 aatctggcag ctggccttca ggatgtcaaa agtcactgtc accagctgct gcctcgtgag
     5401 cgcagccact gctttcctaa gaggcttctc aaataatgca tatggggcac acaacccatg
     5461 tccctcctca ttccatcttt gttcagtgtc aaattcgtac ccaaattaac aactaatatg
     5521 ttcttattcc tcacgcgggg tcagccatga acagcagact cactccggaa gctagcacca
     5581 tgcctttcct atgtctgaca catctagagt cagtaagtcc tgtctgttag atccaatctg
     5641 cacctccgac catcagagca gacattgagt gaaagcaccc agccaccact ttgaagcatc
     5701 cagctggaaa actcaaacag acatggagtt tgctctctgg caaaagcatc accatgtttc
     5761 ccggaccatc agctgaaatt taaaatgcca aagttacaca aaaatagtga tcagtgatgt
     5821 accctggcat gggggtgagc ggggcggcta tgccaggtaa agaggccagg agggcacagt
     5881 gctgctgctc ccaatgccag ccatcccagc tccaggtgga ggcacgtcca cacagcatgt
     5941 ccagaccgtt ccgtagagaa aacccttccg cttttctaac aactcagcat agacacagct
     6001 ccagcaccac cgtagagata tctagaccca gctttcttgt acaaagtggt tgattcgagg
     6061 ctgctaacaa atcgagcagt gtggttttca agaggaagca aaaagcctct ccacccaggc
     6121 ctggaatgtt tccacccaat gtcgagcagt gtggttttgc aagaggaagc aaaaagcctc
     6181 tccacccagg cctggaatgt ttccacccaa tgtcgagcaa accccgccca gcgtcttgtc
     6241 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca
     6301 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag
     6361 cgtcaacagc gtgccgcaga tcttggtggc gtgaaactcc cgcacctctt cggccagcgc
     6421 cttgtagaag cgcgtatggc ttcgtacccc ggccatcaac acgcgtctgc gttcgaccag
     6481 gctgcgcgtt ctcgcggcca tagcaaccga cgtacggcgt tgcgccctcg ccggcagcaa
     6541 gaagccacgg aagtccgccc ggagcagaaa atgcccacgc tactgcgggt ttatatagac
     6601 ggtccccacg ggatggggaa aaccaccacc acgcaactgc tggtggccct gggttcgcgc
     6661 gacgatatcg tctacgtacc cgagccgatg acttactggc gggtgctggg ggcttccgag
     6721 acaatcgcga acatctacac cacacaacac cgcctcgacc agggtgagat atcggccggg
     6781 gacgcggcgg tggtaatgac aagcgcccag ataacaatgg gcatgcctta tgccgtgacc
     6841 gacgccgttc tggctcctca tatcgggggg gaggctggga gctcacatgc cccgcccccg
     6901 gccctcaccc tcatcttcga ccgccatccc atcgccgccc tcctgtgcta cccggccgcg
     6961 cggtacctta tgggcagcat gaccccccag gccgtgctgg cgttcgtggc cctcatcccg
     7021 ccgaccttgc ccggcaccaa catcgtgctt ggggcccttc cggaggacag acacatcgac
     7081 cgcctggcca aacgccagcg ccccggcgag cggctggacc tggctatgct ggctgcgatt
     7141 cgccgcgttt acgggctact tgccaatacg gtgcggtatc tgcagtgcgg cgggtcgtgg
     7201 cgggaggact ggggacagct ttcggggacg gccgtgccgc cccagggtgc cgagccccag
     7261 agcaacgcgg gcccacgacc ccatatcggg gacacgttat ttaccctgtt tcgggccccc
     7321 gagttgctgg cccccaacgg cgacctgtat aacgtgtttg cctgggcctt ggacgtcttg
     7381 gccaaacgcc tccgttccat gcacgtcttt atcctggatt acgaccaatc gcccgccggc
     7441 tgccgggacg ccctgctgca acttacctcc gggatggtcc agacccacgt caccaccccc
     7501 ggctccatac cgacgatatg cgacctggcg cgcacgtttg cccgggagat gggggaggct
     7561 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     7621 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     7681 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     7741 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     7801 gggcggcagg ccctgccata gccactggcc ccgtgggtta gggacggggt cccccatggg
     7861 gaatggttta tggttcgtgg gggttattat tttgggcgtt gcgtggggtc aggtccacga
     7921 cccaagcttg gctgcaggtc gagctcgcga aagcttggca ctggccgtcg ttttacaacg
     7981 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     8041 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     8101 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     8161 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     8221 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     8281 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     8341 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     8401 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     8461 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     8521 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     8581 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     8641 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     8701 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     8761 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     8821 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8881 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8941 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     9001 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     9061 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     9121 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     9181 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     9241 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     9301 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     9361 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     9421 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     9481 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     9541 gttccactga gcgtcagacc ccttaataag atgatcttct tgagatcgtt ttggtctgcg
     9601 cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct
     9661 ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact
     9721 tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat
     9781 taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat
     9841 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     9901 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     9961 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg
    10021 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
    10081 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg
    10141 gccctctcac ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt
    10201 cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg
    10261 aagcggaata tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc
    10321 tgccacatga agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtataca
    10381 ctccgctagc aacctggctt atcgaaatta atacgactca ctatagggag accggcagat
    10441 ccggcgcgcc tgcaggcgcg ccactagtta attatttaaa tcgatgcgac gctagcggcc
    10501 gcgtttgctt ttgtttcgtg ttggtcacca cggccgagtt tcctcgggac cccgggcagc
    10561 tgctttacat cccaaagacc tatctgctcg gccggcctcg gaacgcgagc ctgcccggaa
    10621 gatccccggg taccgagctc gaattcatcg tcaccatcac ctcgaatcaa caagtttgta
    10681 caaaaaagca ggctttaaag gaaccaattc agtcgagacc atggctgtgg tgggcaagga
    10741 catccgtttc acatgctccg cagccagcag cagcagctca ccaatgacct tcgcctggaa
    10801 gaaggacaat gaggtcctgg ccaatgcaga catggagaac tttgcccacg tccgtgcaca
    10861 ggacggcgaa gtgatggagt ataccactat cctgcacctc cgtcacgtca cctttgggca
    10921 cgagggccgc taccagtgta tcatcacaaa ccactttggc tccacatact cccacaaagc
    10981 caggctcact gtgaatggta aggacatggc atctcgtgct tctcaagcac agagtcccac
    11041 agactacact gctgattgtc ctctctgaca ctggtgtgaa gagacacgag ccgtggggtg
    11101 catctctttg taccagtcct tgggtttgtc ccagaccaag tttaacagat tgaaccatcg
    11161 ttttttgttt ggggtgggag atttttaaag tccagttatg ttaagtgaca ttgtggagtt
    11221 aagctttgta tttttatgtt ccttgtcttc cataagccaa gagatgcggt ttctagattc
    11281 caagcatccc taggttctaa ggaagcttgt gaccttagag tagatgttat aactgggagc
    11341 aaggccgttt gggacaagta caagccccac ttcctctaga acaatggttc ttaaccttcc
    11401 taatgcttga ccccttaata tagtccctca tgctgtgctg acccccaacc ataaaactac
    11461 tttctttgct atttcataac tgtaacttcg caactgttat gaatcacagt gtaaatattt
    11521 ttgaacatag aggtttgcca aaggggttca tgatccacag ggtgacccat tgttccgagc
    11581 cgctactctt caggctgtgg agagcaggtg tgggttgagc ccctggctcc gaggtccagt
    11641 gtgcagggaa gttgtgacaa gggtctctta ctcaggagcc agcccctggc catcagggtg
    11701 gctttcttct ggactactga tggatgtggt atgttgaggg atgaggacag catcagtagc
    11761 taactcttcc acactaggct aagctggctt gtgggtggcc tgaagcactg cctccatgct
    11821 ctctgctatt gtacaacacc acaaagcact ctaccacagc gaggctggcc taagagaggc
    11881 cagagtgttt cttggagacc aagtagctgt agactctcca ccacagtagc tcagacatca
    11941 agtctggata ctgtccactg tggcagcatt gatactaaag ctacagaagc gaacgaacta
    12001 catatttttg cctgggtgtg gctggtagtg tagcccccat agaccccacc cagagtgggt
    12061 ttcctcaaga gaggtgagcc agctcactgg ttcctcctgg tgacagcatc cagctatccc
    12121 ctaatagcaa atgttttgtt gcccagctct ctccagatag ctaactaaag caggtcagca
    12181 ccagactttt acttctggag gattccctac atagtaacta ctgtggagct tcgggaggca
    12241 aaacgctcca tcagaagcct tccgagagtg cttagcacca ggctatagca ataggaaggc
    12301 gttaagggta atgggtgcag tattgtgtct gtgacgggga gaggctgggc accccttggg
    12361 tgggatggag taacaatgaa ctgaggcctg ggcacagtgg gatctgttta ctatgagttt
    12421 ccttttgtat gcagccattc caagtagctt tacctatcat ttttttccag tgttgccatc
    12481 attcactaaa ataccccatg acattgccat ccggactggc accacagccc gcctcgagtg
    12541 tgctgccacg ggccacccta accctcagat tgcctggcag aaggatggag gcaccgattt
    12601 cccggcagct cgtgagcgac gcatgcatgt tatgccagac gatgatgtgt tcttcatcac
    12661 tgatgtgaaa atagacgaca tgggggtcta cagctgcact gcccagaact cggcaggctc
    12721 ggtttcagcc aacgctaccc tcacagtctt aggttcgcta cgacagctct cgcgcacgtg
    12781 tggcagggtc gggtggggat tcaactcaag tattagagaa cctgctttct ggagtccctg
    12841 tggtagactc tgtctagtta atttgaattt tggggctgct tttaagcgag tagtttcagc
    12901 tcataaaaga caatgagcta tatgttagtc tgcgtgagaa ttctgtgtgt gtgtgtgtgt
    12961 gcactcacac aggtgggcta tgatcttagg agtagtgaca aaggtctata acaggacagt
    13021 ttgggatagg gtagggcttt agttctttgc tctgttgagc tgttcctaga atggcagtga
    13081 tttaattaca ttaggctttc tggaaagctt ggtgctccaa taaaagatct cagatagcat
    13141 ttcactacaa catgggattg tcttctgtct ggaaaacatg tatacctcca gcaagcgaga
    13201 cccatagaat aaatgtgcaa tttccactgc cttcctgcag aaactccatc cttggcagtg
    13261 cctctggaag accgtgtggt aactgtggga gaaacagtgg ccttccagtg caaagcaacc
    13321 gggagcccca caccacgcat cacctggctt aagggaggtc gcccattgag cctcacagag
    13381 cgccaccatt tcactccagg caaccagctg ctggttgttc agaatgtgat gatagacgat
    13441 gcagggcggt atacctgtga gatgtctaat cccctgggca ctgagcgagc acatagccag
    13501 ctgagcattt tacctacccc tggctgccgg aaggatggga ccaccgtagg catcttcacc
    13561 attgctgtgg tgtgcagtat tgtcctgacc tcgctggtgt gggtgtgcat catctaccag
    13621 accaggaaga agagcgagga gtacagcgtc actaacacag gtcagcagca cagcggactt
    13681 ggggggctgg gggcagatgg ggtgacactg ccgcagtccg aggtaaacga tgcaggcctg
    13741 ggggcttgcc tagaacctga aaggtctcta gaacatttag gaatatggac tctgcctgta
    13801 gagttgggac tttggtccat gtcctccccc ccccccaaca gcaagctagc tctgaaggac
    13861 agaacagaaa atgggaatac tctcacatgc aaggactcgc tggtctctgc agtttctggt
    13921 ctctttggca gctcagaggc cctagggcca cgcctgttat gaaccggatt gttcctaggc
    13981 tctcagtctt ggttaggcct tcctttcctt gttcctcatg gagccaggcg tctgactgcc
    14041 gtgatgttta caccagggtg taaaggaggt taatgagcgg ctgccgagcc tctgcacagc
    14101 tttctgtgtg tgagccactg tcgtgtgacc ttgtgatagt gcgtagttat ctgtatttat
    14161 tgttgtggca gccctgttat tttattgtct tggctccttg cagatgaaac cattgtgcct
    14221 ccagatgttc caagctacct ctcctctcag ggaacccttt ctgaccggca ggaaactgtg
    14281 gtcaggactg agggtggtca tcaggccaat gggcacattg aaagcaatgg taagttcgga
    14341 acagtgagcc agctgccctc gtcctggatg aagtctggca ctgggtcaca tttggtgaac
    14401 caggccttta attgataaga tagtttgctt tggatctggt gtgtgcaagt tctatacttt
    14461 taacgttaga tcctccggaa agtccctgca ctgggcttag cactctgtct tctggtcaga
    14521 aggattatat gataggattg ccagaaagaa tatatgcaaa ggacattcca ggcccccaaa
    14581 tcagcccata gcttttacag tactgtagca ctttacatgg gctaagtata agtcaaccaa
    14641 ccacagactg aaaatattta gcaaagaaaa actgaatcta tatggacatt ttacgtcgtt
    14701 actaggtact atgagtgaag atgacttaag gtctgtcaga gaaggtgtac agtttatgtg
    14761 caaacagcat ttcacagaaa aggaacttgg catccttgga tttgggcatc tcagaggagc
    14821 ataggaacca atccctgggt ggatgccaag gtgctgggaa aagcagtggt ctggtggcca
    14881 tcaggctgcg tgatgtaagc agcttctaag tggtaagggc tctgtttcag gtgtgtgtct
    14941 gagagacccg agcctcttcc cagaggtcga cattcatagc actacatgca ggcagcccaa
    15001 gctgtgtgtt ggatacacta gagagccctg gaaggtgacg gagaaagccg acaggacagc
    15061 tgccccacat acaacaggta ggagacactt aagtcagggg acgcggttgc ttttggccct
    15121 gaagctggct tgactgagta aactagagta gcggcagatc actggccgca cgctgtctct
    15181 gagttatgtt tagaatacaa acaggatttt gtgtgattct agagtcctgt gtcgcttagg
    15241 taagtcagtc tatgatgtgg tgagctgaag gctgagtttt cttaacatgg ctgagttttc
    15301 ttaacataag gaacttacaa gacaaggcct gcccttgcgc caggactgtt gtcccgacaa
    15361 ggtttcactt cgtggatcag gctgtcctca gcctcctgag atgctgtggc tgggctaccg
    15421 tgcccgtccc ttagagtgcg ccttcctcat gtcactcagc tcatgtcctc agtcactctt
    15481 tgtcctgtgt aatgaacagg gattctctct caaacccagg gttcactgat ccaattttct
    15541 agcccgctag cctgctccgg ggtttcctgc ctctgcgtta gctgggccac cacacctgcc
    15601 tggcatttcc aggagtactg gggacctgaa ctcgggtcct tatgctcagc aagcgctttc
    15661 cctactgagc cctccctctc cgcagccctg ctggcgggac tctttaaggc ctactgatta
    15721 agattcctta ctgtatgatg cctaatgcaa accgttcctc ttcctctccc caccctcatt
    15781 ttcctgtgat tagagtgtgc cttcgaaatt aattttacac gtatacttag gcacacaaga
    15841 ctaagacatg gttggtctag ctcctctcca catacacatt ccagggggtg gggaggggag
    15901 agacagggtg gttatgaagg cagtggtaag ctggactcat cttccaatag tgcctctgag
    15961 agggaatgga gactcagttg acctctggtt ctcttcttcc ctcagcacac agtggctctg
    16021 ctgtatgtag tgactgcagt actgacacag cctaccatcc ccagcctgtg cccagagaca
    16081 gtgggcagcc aggcacagcg agcagccaag agctcaggca gcatgaccgg gaatattctc
    16141 cacaccatcc ttacagtggg actgcggatg ggtctcatac cctctctggg gggtccctct
    16201 atccaagcaa ccatgacaga atactgccat ccttgaagaa caaggcggcg tctgcagatg
    16261 ggaacggtgt gtattgtgtt agaggaacct cggtggctgc tgggagccct ttggttacgg
    16321 tgcataggct gagctgcctc tttttttttt ttttttttta aacaggagat tcctcttgga
    16381 ctttagcaaa gttacatgaa gcagactgca tagacctaaa gccttctcct acgttagctt
    16441 caggcagccc agagctcatg gaagacgcca tatctactga agcccagcac ttgcttgttt
    16501 ccaatggcca cctccccaag gcctgtgact ccagccctga atctgtgcca ctgaaagggc
    16561 agatcactgg gaaacggagg ggaccactgc tattggcacc gagaagcggt ttaaactctg
    16621 gttccgaggg cagaggaagt ctgttaacat gcggtgacgt cgaggagaat cctggcccat
    16681 ctggctccat gagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
    16741 tagatggtga tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta
    16801 caaacggaaa actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc
    16861 caacacttgt cactactctg acctatggtg ttcaatgctt ttcccgttat ccggatcaca
    16921 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta
    16981 tatctttcaa agatgacggg acctacaaga cgcgtgctga agtcaagttt gaaggtgata
    17041 cccttgttaa tcgtatcgag ttaaagggta ttgattttaa agaagatgga aacattcttg
    17101 gacacaaact cgagtacaac tttaactcac acaatgtata catcacggca gacaaacaaa
    17161 agaatggaat caaagctaac ttcaaaattc gccacaacgt tgaagatggt tccgttcaac
    17221 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
    17281 accattacct gtcgacacaa tctgtccttt cgaaagatcc caacgaaaag cgtgaccaca
    17341 tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat gagctctaca
    17401 aaggaggatc tggcggccgc tctgccacaa acttcagcct gctgaagcag gccggcgacg
    17461 ccgctgaaaa tcctggccct ggaagcacca acctgctgac tgtgcaccaa aacctgcctg
    17521 ccctccctgt ggatgccacc tctgatgaag tcaggaagaa cctgatggac atgttcaggg
    17581 acaggcaggc cttctctgaa cacacctgga agatgctcct gtctgtgtgc agatcctggg
    17641 ctgcctggtg caagctgaac aacaggaaat ggttccctgc tgaacctgag gatgtgaggg
    17701 actacctcct gtacctgcaa gccagaggcc tggctgtgaa gaccatccaa cagcacctgg
    17761 gccagctcaa catgctgcac aggagatctg gcctgcctcg cccttctgac tccaatgctg
    17821 tgtccctggt gatgaggaga atcagaaagg agaatgtgga tgctggggag agagccaagc
    17881 aggccctggc ctttgaacgc actgactttg accaagtcag atccctgatg gagaactctg
    17941 acagatgcca ggacatcagg aacctggcct tcctgggcat tgcctacaac accctgctgc
    18001 gcattgccga aattgccaga atcagagtga aggacatctc ccgcaccgat ggtgggagaa
    18061 tgctgatcca cattggcagg accaagaccc tggtgtccac agctggtgtg gagaaggccc
    18121 tgtccctggg ggttaccaag ctggtggaga gatggatctc tgtgtctggt gtggctgatg
    18181 accccaacaa ctacctgttc tgccgggtca gaaagaatgg tgtggctgcc ccttctgcca
    18241 cctcccaact gtccacccgg gccctggaag ggatctttga ggccacccac cgcctgatct
    18301 atggtgccaa ggatgactct gggcagagat acctggcctg gtctggccac tctgccagag
    18361 tgggtgctgc cagggacatg gccagggctg gtgtgtccat ccctgaaatc atgcaggctg
    18421 gtggctggac caatgtgaac attgtgatga actacatcag aaacctggac tctgagactg
    18481 gggccatggt gaggctgcta gaggatgggg acctcgagcc atctgctgga gacatgagag
    18541 ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac agcctggcct
    18601 tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc cccatactct
    18661 attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc ttactgacca
    18721 acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg ccaggctttg
    18781 tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta gagatcctga
    18841 tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt gctcctaact
    18901 tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc
    18961 tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag tttgtgtgcc
    19021 tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc accctgaagt
    19081 ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac actttgatcc
    19141 acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg gcccagctcc
    19201 tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat ctgtacagca
    19261 tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg gacgcccacc
    19321 gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac caaagccact
    19381 tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc acgggggagg
    19441 cagagggttt ccctgccaca gcttgaggcg cgccggatcc attatgtacc tgactgatga
    19501 agttcctata ctttctagag aataggaact tcgaagggtt ccgcaagctc tagtcgagcc
    19561 ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag catgcctgct
    19621 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
    19681 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
    19741 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
    19801 agaaggcaca gtcgaggctg atcagcgagc tctagagaat tgatcccctc agaagaactc
    19861 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
    19921 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
    19981 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
    20041 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcatc
    20101 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
    20161 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
    20221 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
    20281 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
    20341 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
    20401 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
    20461 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
    20521 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
    20581 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
    20641 ggccgatc
//
