LOCUS       sequence-247127-       16714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B6J Hipp11 targeting vector homology arms, Capecchi configuration, 
            DTA, I-CeuI, forward cloning sites, low copy number.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Mouse safe harbor locus targeting plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pB6-Hipp11-DTA-Capecchi-for
FEATURES             Location/Qualifiers
     source          1..16714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     149..168
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             243..815
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          892..957
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(1025..1042)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1031..1255
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1317..1337)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1317..1335)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1372..1391)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1416..1464
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(1768..1785)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1768..1784)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1777..1799)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1993..2012)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2112..2134
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2172..2190)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2258..2362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2363..2431,2432..3223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2581..2600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3388..3933
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     3834..3853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4050..4067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4217..4236
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4217..4235
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4468..4492
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10766..11164
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(16200..16224)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(16431..16714,1..216)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(16466..16489)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
       61 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
      121 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
      181 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcaggtcctc gccatggatc
      241 ctgatgatgt tgttgattct tctaaatctt ttgtgatgga aaacttttct tcgtaccacg
      301 ggactaaacc tggttatgta gattccattc aaaaaggtat acaaaagcca aaatctggta
      361 cacaaggaaa ttatgacgat gattggaaag ggttttatag taccgacaat aaatacgacg
      421 ctgcgggata ctctgtagat aatgaaaacc cgctctctgg aaaagctgga ggcgtggtca
      481 aagtgacgta tccaggactg acgaaggttc tcgcactaaa agtggataat gccgaaacta
      541 ttaagaaaga gttaggttta agtctcactg aaccgttgat ggagcaagtc ggaacggaag
      601 agtttatcaa aaggttcggt gatggtgctt cgcgtgtagt gctcagcctt cccttcgctg
      661 aggggagttc tagcgttgaa tatattaata actgggaaca ggcgaaagcg ttaagcgtag
      721 aacttgagat taattttgaa acccgtggaa aacgtggcca agatgcgatg tatgagtata
      781 tggctcaagc ctgtgcagga aatcgtgtca ggcgatctct ttgtgaagga accttacttc
      841 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
      901 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
      961 ccaacctatg gaactgatga atgggagcag tggtggaatg cagatcctag agctcgctga
     1021 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1081 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1141 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1201 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     1261 gaggcggaaa gaaccagctg gggctcgagg gggggcccgg tacccagctt ttgttccctt
     1321 tagtgagggt taattgcgcg cacgtttgcc cgggagatgg gggaggctaa ctgaaacacg
     1381 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     1441 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     1501 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     1561 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     1621 ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga atggtttatg
     1681 gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgacc caagcttggc
     1741 tgcaggtcga gctcgcgaaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     1801 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     1861 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     1921 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     1981 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     2041 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     2101 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     2161 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2221 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2281 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2341 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2401 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2461 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     2521 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2581 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     2641 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     2701 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     2761 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2821 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2881 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     2941 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3001 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3061 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3121 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3181 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3241 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3301 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3361 gtcagacccc ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg
     3421 ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa
     3481 ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt
     3541 ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg
     3601 ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata
     3661 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     3721 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
     3781 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac
     3841 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg
     3901 tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt
     3961 ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt
     4021 ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata
     4081 tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag
     4141 cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcaa
     4201 cctggcttat cgaaattaat acgactcact atagggagac cggcagatcc taactataac
     4261 ggtcctaagg tagcgaaatc gatgcgacgc tagcggccgc gtttgctttt gtttcgtgtt
     4321 ggtcaccacg gccgagtttc ctcgggaccc cgggcagctg ctttacatcc caaagaccta
     4381 tctgctcggc cggcctcgga acgcgagcct gcccggaaga tccccgggta ccgagctcga
     4441 attcatcgtc accatcacct cgaatcaaca agtttgtaca aaaaagcagg ctttaaagga
     4501 accaattcag tcgagcaaat agaatttaag gccagcctgg ggccttgtga gggcctactg
     4561 tgaccctcca aaaaataaaa caagattagc tgttgtggga aagtagaagg acaaatgtga
     4621 ggcaggagat gagagaggaa tgactggtca catgatggga gtccagtgaa catgctggca
     4681 ggtctgtaaa ttgttctctt atgggtttat ttgtgatgat acatctagct ttaccagtgg
     4741 gttgggattt ttgtttttgt ttttaactga tacagactta tccaatcaaa actgatagac
     4801 aaggggacta gaatagttct gtgcttcagg actccatatg aaaccctgca ctaaatatcc
     4861 caaaggctct tgctttagag aaactccaga aggaattttg ttcatgagtt aggccctgtc
     4921 tcatccttta taaaattcat tataaaatta tataatttca ttataaaata aacaagaaca
     4981 aaggatcctt aatggtaagt tctagtaagg tgtacgataa ttgagttttc atgtatgtaa
     5041 ttttaaaaga aaaatacaag gaacaagtgg aatcataggt tctgaggtct ctgaaacatc
     5101 tcttccccat tactaaggta tatttccagc catgctcttc cctcttctta gccctatttc
     5161 agggttgaga aacatgagaa cacacacagg cccattttac agggtggcaa agatggattg
     5221 aagttcttgg gacaatgact acttgcccct ttgtgttctc ttgtagtagc tataggtcag
     5281 aacattagaa ttacctctgc ccaagtaaac aaaatgggaa ctctttaccc tgttgaagat
     5341 ggcttcacct ggtaaagcta ggcttaagag cgagtgctgc atagcccagt ggtcaagtgc
     5401 tccaaagcat atgtaagacc ctaggcttaa ttcccagtgt cctgaaaaga taaagcactg
     5461 gcttagtgtt aggcaaaggc acttgagtgt gctgatctct gcctcttctc tgcagctttc
     5521 tccttccttc acccctacct cagtgatccg gaagatgtat gaaagcagag agaaaaccaa
     5581 ggaggaaatg gcacctggaa tggtggttcc tggtgatggt aaagaggata ctcagaagac
     5641 cagcgaaggt actgctgctt acctgtgtca gaacataggc atgagtgact gcagtgtcag
     5701 ggagatgggt tagtgagctc aaacttgctc acttgacatt gtggccagcc ggggtaacga
     5761 tgtgctttag cttatgattc ttaccatctt tcaagttgaa taacaaactc atttagcata
     5821 ttgatatagc tcaattcttt ttttaacgta tttgtgtatt tgcctgtggt atgtctgcat
     5881 catgtgtatc cagtgcccaa ggaggccaga agagggcatc agaacctcta gaattgtgag
     5941 ccacatgtgg gtgctgggaa gaggtcctct tcaagagtat ccaggtagat agggggatag
     6001 atgcagggtt gttgttgttt ctgttacagg ttgtttgttt tatttgcaac aggatcttac
     6061 tgtgtagctg tggctaacct caaactcaca gagatctact ggtctctgcc tcccaagtct
     6121 taggattaaa ggtgcctcaa ctacatgcct ggcaacttgt gctttttgtt ttgtaacact
     6181 atgtcagagg tttttgagtt attcctttat gcaccgggaa tgcagctcaa ttgtactaag
     6241 tagtagaata cttgccagca tgtacaagac catgggttca tgttcactcc ccaagaaagg
     6301 aaatgacaac atcaaaaata attatttcag caaagaattt gaagaccttg gcatctatgg
     6361 actggagaga tagatcaggg cactgtttgc tcttccagag gacctgggtt caattgccag
     6421 cacccacatg atagctcacc ttttgtgact tcagccctag gtgacctagc accctcccct
     6481 ggcctctgtg agcactgaac tcgacaggca cacagacata catacaggca aaacaccatc
     6541 catatacatc ataatataag aaaataaggt tataaataaa atgtgggcat ctattcctag
     6601 ctcaaggcct aactactcta tgaccataac tcaactccgg ggcttatgga gagtaccagc
     6661 tgtgcatctg tctacattta aagcaaatag atgactttcc atgaaagcat ctaacatggt
     6721 taagaggaaa aaagagccgg catggtggca cacgccttta atcccagcac ttgggaagca
     6781 taggcaggtg gatttctgag ttcgaggcca gcctggtcta caaagtgagt tccaggacag
     6841 ccagggctat acagagaaac catgtcttga aaaaccaaaa aaaaaaaaaa ggaaaaaaga
     6901 attgtcagaa tttttaaaag taagatttgc tgggttgttg ggaattgaaa taaattgata
     6961 aaggatatca aaatgtcctg taaatgaatc cttacagaga acctgctgtc atccaatccc
     7021 atacccaata ctgatcaaga ctcttctacg acaaatccta agctttcaac attacagcgg
     7081 tcttcatgtt ccaccccact ctcccagacc agccgttata ccaaagaaca agattatcga
     7141 cctaaaacag caggaaggaa aacacctacc ttggcatccc cagtcccagg aacacctttt
     7201 ctccgcccta cccaccaagt tccccttgtc cctcatgttc ctattgttcg acctgcacac
     7261 cagcttcacc caggattagt ccaaaggttg atagcccaag gggtacatcc acagcatctt
     7321 ccaagtttgc tccaagctgg tgagtttctg actgggtgtg ggcattggtt attggtcgta
     7381 gtataaaaat tgctgccact aattaaatgt tacattatac taagtattcc aaaaagatct
     7441 tactagattt aaattcttct acctaagtag tgagtccaat ccagtctcac ttccttagct
     7501 cacaaaaaga ttaggaaacc tgccagacag tggtaataca cgcctttaat ccctgcactc
     7561 aggaagcaga ggcaggtgga tctctttgag ttcaaggata gccagggtac actaaggaac
     7621 cctggctgtg gcgttcattt acaattgtgt ctgtgtatgc atattcatgt accctggtac
     7681 atgtatggtg gcaagtgttc accacatcca cctccttgta agtatccaaa tcaacaaaag
     7741 tagtaaactt tgacattttt aggaacatct acaactcatg agtttcttct ttctccccat
     7801 caggtgtgct tcctcctggg atagacatgg ctcctttaca ggggctatct ggcccactcc
     7861 tgggtcaacc cttgtaccct ttggtctctg ctgctagtca ccctctccta aaccctcgtc
     7921 ctgggacccc tctgcatttg gcggtgatgc agcagcagct acagcgctca ggtaaggttt
     7981 agaaataggc caggttagct gatcacactg gaaagttctg agatttccag gagcaatagt
     8041 tatcactgcc cctttgtttc ttaggtgtca ctagaaaaac aggtgaagtg aactgacctg
     8101 tgcagtctca gggattatta ctactgcaag gacagtcgga gccagccatg ctctagctct
     8161 gtttttcact tcttataagc ctgagaattt ttgctgagat gtgaacatgt cagccttggt
     8221 gggctttctt acgttgcatt ttcttacgtt gcattttgca ctttaggaag cctttggtta
     8281 aatcctctgg tagacttccc atcccccaaa gtaacaaacc attcaagcaa agtaggtcag
     8341 aaaatattgt caatactgag aacaggactt gggagtgagt atccaagggt ggtcttagta
     8401 gacttgtggg gttctgtggg tgaggtgtag agtagagcta ctttgctgca ctgacactct
     8461 tctctccata gttctgcatc ctccaggctc tagttcccag gcagcagcta tcagcgttca
     8521 gactcctcag aatgtaccca gccggtcggg catgccccac atgcactccc agctggagca
     8581 tcgtaccagc caaaggagca gctcccctgt gggccttgcc aaatggtttg gctcagatgt
     8641 gctacagcag cctctgccct ccatgcccac caaagtcatc agtgtagatg aactggaata
     8701 tagacagtga agagggcagg ctggctcacc catacctgga cctgtggtga caccctggtc
     8761 atgactctca ttccctcttt gtaatgggct tttacattgg agcacactat gtgaagatgt
     8821 ttaggggatc cacataccta ccatgatcta cattatgaca gaaggctgtt aaatcgaatg
     8881 aacctacatg gttcaaatac aagggataca agattgtcag tcctggaagt ctttctttta
     8941 taaaatatgt gaatgaagtg ttggtgtctt ctagaggtga cacctaaggg ttctgaaaaa
     9001 ataaaatgta tagaccctta tgtacagacc tgtgtataaa cttttgtaca tacaaatagg
     9061 gtagcttttt ttgaacttat acatacagct gtacataaag taactatcag ttaggcttgt
     9121 gtcaactgtt tggatttttt tcacttgaat atttgggact ttttcttttg gtttattaaa
     9181 agttacatat gccacgtgtg tgaacgatat ggctggtact gtgtttattt cttccatgaa
     9241 ctaagacagt ctaaatgagt tcctttcacg ttttaatttt accttaggac ttctggaatt
     9301 tcttctgcac ataaagttct gatagcatta gtttaagctg gactaaccct gaaagtagct
     9361 tgtggcaagt atcaaggaat caatattata ctctacaaaa tcaaagttta cagagaagtc
     9421 atatagtaat ttttctgaaa tttactggca caatgttaat ccagcctgac tccaactaat
     9481 taatggtcac attaatttaa gtctttccct tgcctctgct gcattagttt ctctcaaaat
     9541 tgttaactta caacttgaag tctggtatta taaattgaat gtaaagcatt ctgaaagata
     9601 ctatactgat tgcaggtttt tcagtcaggt tcaagctaat ttgaccagtc attggattaa
     9661 ttatggatct ggggccataa atgctatttt aattccacta tagagattaa aataagccat
     9721 tctccatttc ataatattct attggacttt gactgcaggg gcctccaagt cttgacagta
     9781 gattataatc cttcagctgc ccactctact ggaggaggac aaactggtca cttttcagca
     9841 aaacctggct gtggatcagg gcagtctggt acttccaagc tcattagatg ccatcatgct
     9901 ctcactgcct cctcagcttc aagaggaatc tggaaaaagc agtcccactg gtcaggaaag
     9961 gaacactagt gcacttatcc tgggtgtctg ctgagccaac agtggtagta aggtaagggc
    10021 aggatgtgtc aaactgccaa tagagaacta cttactcttc aggctgaagc tgatggaaca
    10081 ggtaacaaag gcaaacacta atcatgatca gcaagatgaa gcagaaaggg aacaagggga
    10141 tattaaatgt gtatagacac gctagagaga tggctcagca gttaagagaa ctagctggtc
    10201 tttcagaggt cctgagatca atggcgcgcc agtcttaatt aaggcctggt gatgatggcg
    10261 ggatcgttgt atatttcttg acaccttttc ggcatcgccc taaaattcgg cgtcctcata
    10321 ttgtgtgagg acgttttatt acgtgtttac gaagcaaaag ctaaaaccag gagctattta
    10381 atggcaacag ttaaccagct ggtacgcaaa ccacgtgctc gcaaagttgc gaaaagcaac
    10441 gtgcctgcgc tggaagcatg cccgcaaaac gtggcgtatg tactcgtgta tatactacca
    10501 ctcctaaaaa accgaactcc gcgctgcgta aagtatgccg tgttcgtctg actaacggtt
    10561 tcgaagtgac ttcctacatc ggtggtgaag gtcacaacct gcaggagcac tccgtgatcc
    10621 tgatccgtgg cggtcgtgtt aaagacctcc cgggtgttcg ttaccacacc gtacgtggtg
    10681 cgcttgactg ctccggcgtt aaagaccgta agcaggctcg ttccaagtat ggcgtgaagc
    10741 gtcctaaggc ttaaggagga caatcatggc caagcctttg tctcaagaag aatccaccct
    10801 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
    10861 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
    10921 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
    10981 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
    11041 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
    11101 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
    11161 ctaaggcgcg ccagtcttaa ttaatttaga cacccacatg gtggctcatg accatctatc
    11221 tataaatgga tctgattttc atgtctggca gtgtacagaa gctaactgaa gaaaggtgga
    11281 agacccacaa gagttcaaga taagccctat atagtgaagt tcaaggcaag ctttttctac
    11341 ctgaaactta gtctcaaaaa aaaatgaata cgtaaacagt cttccagggg ataagaacct
    11401 tacagaaaaa gcagaaatgc ctggggcact ggattaccga tgtaatcaaa ttcagtcctt
    11461 gaattgaaca caggattgcc tagagcaagg ccagccagag attcatctca gagggagaaa
    11521 ggtgtctttg gagcaatttt gtggtaatct agtatgtatc acataagttt agacgcattt
    11581 gggactggaa agatgtgaac aaagcaccct atggctcaca tctgtcatta actctagttc
    11641 cagtgcacct gacaccgtct tctggcctct gcagtgacca agcacatggg tagtatgtag
    11701 acatatacat aagcaaaaca cacatcatta aaaagtgaca tttcccaaag gaagctgaag
    11761 aaccagttct tgagaagata gtagaaatca gaaggggaaa tagtagacat acagagggac
    11821 tgaccaggtt gtgtcacctt tataggctag gctaatggat gatcgacact agcgctcttt
    11881 gtgaaggaca cacaaatgag acatagttta taggactaaa cacacttcta agcaatttaa
    11941 tgagacttaa gaccctgtct ctagcaaata ctctggatga tattcagctc aaggctcttg
    12001 tcagacatgt ttccattttc aaggtgagct aactggccaa aactgccaac aacctgtagt
    12061 gaatagagaa gatgagaaaa tctggattct caaatgacct aatgaaagcc actggagcgc
    12121 catatggttt ctgtgaaaat gccttttcaa tcattaacct cttaaatgag tgttagcatc
    12181 ctaactaatg agtggtgcag aatagtgggt ctgcttagct taactaaggc caagaaaaca
    12241 aaacaggaaa ttcattccat gtcatgagac tcatactacg aggttccctt agacctcagg
    12301 agaaaaagtc tttggctgta agaacacacc tcagtggatg tggtagacta tgcctttact
    12361 cttttttttt tttttttttt tttttgggtt ttttcgagac agggtttctc tgtgtagcct
    12421 tggctgtcct ggaactcact ttgttgacca ggctggcctc gaactcagag atccgcctgc
    12481 ctctgcctct caagtgctag aattaaaagt gtgcgccacc actcccagct atatagacta
    12541 tgcctttaat caggacactt gggaggcagg gggatctcaa attcaattcc agacagatga
    12601 ctgacaaaca cacacataca ccattcgtct tttctttttt tcttttttct tttgaaaaca
    12661 ggtttctcta tgcagccctg gctttcctag aattcaatct atagacccag ctggcctcaa
    12721 acacagaacc tcttgcctct gcctcctcaa cactatgact aaaggtgtga accaccacca
    12781 ccaccaccca cctggccaaa gaatgaattg agtgaataag tacgagtgca actctccagc
    12841 agcctgaggc aagaaagctt aacagttgaa cctgagaccc tacttcgttt gtgttgtaca
    12901 tatcttctca taatgaacta ggcagcctag tctctctacc aaatacacca tgcacttaca
    12961 cctcctcaaa ccaactgcaa cagctacaca catttccatg gcttgcctgt aataataaat
    13021 tcacaaactt cctctaagta acttttagtg gaaatctacc aggttatgcc tatttgtgat
    13081 tgggtgcaca gatgactaga tgagatgtaa tttgggaagt gacatgcaat aggaaaaatg
    13141 aatatataac ctataaatag aaaacaccaa ggcaagcgtg gtgactcaca cctttaatta
    13201 gcacttggga ggcagaagtc gcatcatctc ttaggtcaag gccaatctag ttccagacca
    13261 gcaaggacaa cagaaacctt gccttgatat aaacatttta aaactttaaa aatagagaag
    13321 cccaaactct gctcattggt aatctaccct tcctttcttt agacaccttg agaagctagg
    13381 ctacatagct ttgggtcctt acgtaccccc actcactcag cctgcagtca atctctgata
    13441 gtgtttcctg ccataagcta cactactgtt ttgctccaaa ccttacttct agtacaaatc
    13501 catgtgaggt cttcgtttca atttgggggg gaagaggcca gaacacttgc cgcagatccc
    13561 aacaacgggc agcacattcc cagctagtgt atctgataca aactctatct tcattcagaa
    13621 ccatcaagtc taagccccaa gagcaaaggt aaattcatgt atgtttggct ggagaaagca
    13681 tttcaagtca aggtgagcct tgaactccta acactcttgc ctctaccttc caaatgccaa
    13741 tataggtatt tgtcacacca gcttctcaaa tcctgttggg ctagcaactg ggtgggagga
    13801 gtgtagaggg gtactaaaaa atggactcaa ggcctcttca aaaccactga ggccttgtac
    13861 aggatttgaa cttccttctc ttttcagcat cctgaagtac aaagctaaag caaagtaaat
    13921 ttttgcttgg cctgaggact tccaaaaatc taggctctca ctggagaaga aacctagctg
    13981 gaaattccac agttctgata agccaattct tttttttttt aaccatttat ttatttataa
    14041 gtaagtacac tgtagctgtc ttcagacatc ccagatctca tgacggatgg ctgtgagcta
    14101 ccttgtggtt gctgggattt taactcagga ccttcagaag agcagtcagt gctcttaacc
    14161 agtgctctcc ggcccagtgg aaaccaattc tttagtgtgg ggacaggctt tcagtcatag
    14221 cctatcagtc aatgtcctag tgagaaacca gtctatattt tgaggcttga gaggcctgat
    14281 tccaaggaca gttttaaacc cggagaaagg aagagagcta gctctagggc agcagacgga
    14341 gccctgaggg aagggtagtg tgtggtaagg cccacagctt tcctcagacc tctgaaggct
    14401 ctagctcatc tcctttgaag acagaagaag caaagtctgt tctcctacaa agacgttcct
    14461 gtctttctca aggcctcctc tatcatcact gcagaccaca ttaaaataat cttccagcca
    14521 ggcggtggtg gcacacacct ttaatcccag cacttgggag gcagaggcag gccggatctc
    14581 tgagtttgag gccagcctgg tctacaaagt gagttccagg acagccagag ctatacagag
    14641 aaaccctgtc tcgaaaaacc aaaaaaaaaa aaaaaaaaaa aaaaaannga ttactgtatt
    14701 tgtgagcctc aatctcatcg tgtcgtacat ggaacagtgg cattaacttt taaggttggg
    14761 attgctggta ttaaggggag ggttttggaa gctgcttagc acagtgtcag tcgtaggaaa
    14821 tattcactgt atggtggttc ttgtcactca gcctgttttt gtttttgttt tgttttttgt
    14881 ttttttcctg agacagggtt tctctgtagt gtttatatat atatatatat atataacttc
    14941 agacagcaaa agagggtatc agaaagggct aatagacaac ttgcacctca ccctataggt
    15001 ggaacaacag tatgaactaa ccagtacccc ccggagctcg tgtctctagc tggatatata
    15061 gcagaagatg gcctagatgg ccatcagtgg aaagagaggc ccattggtct tacaaacttt
    15121 atgtgcctca gtacaaggga acaccagggc caagaaagcg aggggagggg ggttgggaga
    15181 ctttcagagt agcattggaa atataaatga agaaaatacc tcctcatcac agcagtccgc
    15241 tcagctcaga atccagagac tattgcgctg gagaaaaatc tgcagttaac tttacctatt
    15301 ataccttcct ctctccaatg tcctgagaag gaccacttga aaggacagaa agccaagccc
    15361 aggtgaaagc ctatcattac agcacactac caatggacaa acggacacag gtcccattct
    15421 ctgtcccatt tcctatgacc aagcaggcgt tgcctcattc acctcatcac acagcaaacc
    15481 tcacatgaat aacgacacat ggccagcggg cccaccagct tctttataca gttcaataga
    15541 agtgacagca aagtagggaa agtggtgttg ctgaggagct aaaggtgcac agtggtaagc
    15601 ccagcactga ggagatggag gctgatgaaa gggattctag gccagcctca gctgcaagag
    15661 ttcaaagcca gcctgaaata cataacaccc tatctccgga aagaaaaaaa ttgtggtgtt
    15721 tcagtttgat aagtctccat atcaatccct gtatcccaat ccagaggctg ccaaagacag
    15781 aggggctgag gaaggtactt agtcataagg aacactgctg tggccagcca gcagatggca
    15841 agaggacttc acttcttcac aatctgaatg acgtcttcat cttccaatgt gtggtcttta
    15901 cccactttct gaggattatg tttcacagac agaccccaga ccaaagcact gtatggagag
    15961 gaataaagag tcagtggctg tagcaagcag ctgcttccct aactgcccag gtgccagctt
    16021 atcaaattta gcatcctgga tcttaaaagg cacagaacta caacagataa aagccctctt
    16081 ctaaatacat cagaataaac aaacagtcaa agccacttgc taaagcagtg ttgcacactg
    16141 agtcatggga tcgctgtcat tcaacagcca attagtttaa accaactgga gatatctaga
    16201 cccagctttc ttgtacaaag tggttgattc gaggctgcta acaaatcgag cagtgtggtt
    16261 ttcaagagga agcaaaaagc ctctccaccc aggcctggaa tgtttccacc caatgtcgag
    16321 cagtgtggtt ttgcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
    16381 ccaatgtcga gcaaaccccg cccagcgtct tgtcattggc gaattctacc gggtagggga
    16441 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
    16501 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
    16561 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
    16621 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
    16681 tctcgtgcag atggacagca ccgctgagca atgg
//
