LOCUS       sequence-247133-       15787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B6J Hipp11 targeting vector homology arms, Capecchi configuration, 
            DTA, I-CeuI, reverse cloning sites, low copy number.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15787)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Mouse safe harbor locus targeting plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pB6-Hipp11-DTA-Capecchi-rev
FEATURES             Location/Qualifiers
     source          1..15787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(7036..7060)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7292..7311)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7293..7311)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7461..7478)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7595..8140)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7675..7694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8305..9096,9097..9165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8928..8947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9166..9270)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9338..9356
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9394..9416)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9516..9535
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9729..9751
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9743..9760
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9744..9760
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    10064..10112
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     10137..10156
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     primer_bind     10191..10211
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10193..10211
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    10273..10497
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     10486..10503
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     intron          10571..10636
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(10713..11285)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     promoter        complement(11312..11811)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11360..11379)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     11753..11776
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    12018..12042
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcctggtca acaaagtgag ttccaggaca gccaaggcta cacagagaaa ccctgtctcg
       61 aaaaaaccca aaaaaaaaaa aaaaaaaaaa aaagagtaaa ggcatagtct accacatcca
      121 ctgaggtgtg ttcttacagc caaagacttt ttctcctgag gtctaaggga acctcgtagt
      181 atgagtctca tgacatggaa tgaatttcct gttttgtttt cttggcctta gttaagctaa
      241 gcagacccac tattctgcac cactcattag ttaggatgct aacactcatt taagaggtta
      301 atgattgaaa aggcattttc acagaaacca tatggcgctc cagtggcttt cattaggtca
      361 tttgagaatc cagattttct catcttctct attcactaca ggttgttggc agttttggcc
      421 agttagctca ccttgaaaat ggaaacatgt ctgacaagag ccttgagctg aatatcatcc
      481 agagtatttg ctagagacag ggtcttaagt ctcattaaat tgcttagaag tgtgtttagt
      541 cctataaact atgtctcatt tgtgtgtcct tcacaaagag cgctagtgtc gatcatccat
      601 tagcctagcc tataaaggtg acacaacctg gtcagtccct ctgtatgtct actatttccc
      661 cttctgattt ctactatctt ctcaagaact ggttcttcag cttcctttgg gaaatgtcac
      721 tttttaatga tgtgtgtttt gcttatgtat atgtctacat actacccatg tgcttggtca
      781 ctgcagaggc cagaagacgg tgtcaggtgc actggaacta gagttaatga cagatgtgag
      841 ccatagggtg ctttgttcac atctttccag tcccaaatgc gtctaaactt atgtgataca
      901 tactagatta ccacaaaatt gctccaaaga cacctttctc cctctgagat gaatctctgg
      961 ctggccttgc tctaggcaat cctgtgttca attcaaggac tgaatttgat tacatcggta
     1021 atccagtgcc ccaggcattt ctgctttttc tgtaaggttc ttatcccctg gaagactgtt
     1081 tacgtattca tttttttttg agactaagtt tcaggtagaa aaagcttgcc ttgaacttca
     1141 ctatataggg cttatcttga actcttgtgg gtcttccacc tttcttcagt tagcttctgt
     1201 acactgccag acatgaaaat cagatccatt tatagataga tggtcatgag ccaccatgtg
     1261 ggtgtctaaa ttaatggcgc gccgactagt cttaattaac gcgccattga tctcaggacc
     1321 tctgaaagac cagctagttc tcttaactgc tgagccatct ctctagcgtg tctatacaca
     1381 tttaatatcc ccttgttccc tttctgcttc atcttgctga tcatgattag tgtttgcctt
     1441 tgttacctgt tccatcagct tcagcctgaa gagtaagtag ttctctattg gcagtttgac
     1501 acatcctgcc cttaccttac taccactgtt ggctcagcag acacccagga taagtgcact
     1561 agtgttcctt tcctgaccag tgggactgct ttttccagat tcctcttgaa gctgaggagg
     1621 cagtgagagc atgatggcat ctaatgagct tggaagtacc agactgccct gatccacagc
     1681 caggttttgc tgaaaagtga ccagtttgtc ctcctccagt agagtgggca gctgaaggat
     1741 tataatctac tgtcaagact tggaggcccc tgcagtcaaa gtccaataga atattatgaa
     1801 atggagaatg gcttatttta atctctatag tggaattaaa atagcattta tggccccaga
     1861 tccataatta atccaatgac tggtcaaatt agcttgaacc tgactgaaaa acctgcaatc
     1921 agtatagtat ctttcagaat gctttacatt caatttataa taccagactt caagttgtaa
     1981 gttaacaatt ttgagagaaa ctaatgcagc agaggcaagg gaaagactta aattaatgtg
     2041 accattaatt agttggagtc aggctggatt aacattgtgc cagtaaattt cagaaaaatt
     2101 actatatgac ttctctgtaa actttgattt tgtagagtat aatattgatt ccttgatact
     2161 tgccacaagc tactttcagg gttagtccag cttaaactaa tgctatcaga actttatgtg
     2221 cagaagaaat tccagaagtc ctaaggtaaa attaaaacgt gaaaggaact catttagact
     2281 gtcttagttc atggaagaaa taaacacagt accagccata tcgttcacac acgtggcata
     2341 tgtaactttt aataaaccaa aagaaaaagt cccaaatatt caagtgaaaa aaatccaaac
     2401 agttgacaca agcctaactg atagttactt tatgtacagc tgtatgtata agttcaaaaa
     2461 aagctaccct atttgtatgt acaaaagttt atacacaggt ctgtacataa gggtctatac
     2521 attttatttt ttcagaaccc ttaggtgtca cctctagaag acaccaacac ttcattcaca
     2581 tattttataa aagaaagact tccaggactg acaatcttgt atcccttgta tttgaaccat
     2641 gtaggttcat tcgatttaac agccttctgt cataatgtag atcatggtag gtatgtggat
     2701 cccctaaaca tcttcacata gtgtgctcca atgtaaaagc ccattacaaa gagggaatga
     2761 gagtcatgac cagggtgtca ccacaggtcc aggtatgggt gagccagcct gccctcttca
     2821 ctgtctatat tccagttcat ctacactgat gactttggtg ggcatggagg gcagaggctg
     2881 ctgtagcaca tctgagccaa accatttggc aaggcccaca ggggagctgc tcctttggct
     2941 ggtacgatgc tccagctggg agtgcatgtg gggcatgccc gaccggctgg gtacattctg
     3001 aggagtctga acgctgatag ctgctgcctg ggaactagag cctggaggat gcagaactat
     3061 ggagagaaga gtgtcagtgc agcaaagtag ctctactcta cacctcaccc acagaacccc
     3121 acaagtctac taagaccacc cttggatact cactcccaag tcctgttctc agtattgaca
     3181 atattttctg acctactttg cttgaatggt ttgttacttt gggggatggg aagtctacca
     3241 gaggatttaa ccaaaggctt cctaaagtgc aaaatgcaac gtaagaaaat gcaacgtaag
     3301 aaagcccacc aaggctgaca tgttcacatc tcagcaaaaa ttctcaggct tataagaagt
     3361 gaaaaacaga gctagagcat ggctggctcc gactgtcctt gcagtagtaa taatccctga
     3421 gactgcacag gtcagttcac ttcacctgtt tttctagtga cacctaagaa acaaaggggc
     3481 agtgataact attgctcctg gaaatctcag aactttccag tgtgatcagc taacctggcc
     3541 tatttctaaa ccttacctga gcgctgtagc tgctgctgca tcaccgccaa atgcagaggg
     3601 gtcccaggac gagggtttag gagagggtga ctagcagcag agaccaaagg gtacaagggt
     3661 tgacccagga gtgggccaga tagcccctgt aaaggagcca tgtctatccc aggaggaagc
     3721 acacctgatg gggagaaaga agaaactcat gagttgtaga tgttcctaaa aatgtcaaag
     3781 tttactactt ttgttgattt ggatacttac aaggaggtgg atgtggtgaa cacttgccac
     3841 catacatgta ccagggtaca tgaatatgca tacacagaca caattgtaaa tgaacgccac
     3901 agccagggtt ccttagtgta ccctggctat ccttgaactc aaagagatcc acctgcctct
     3961 gcttcctgag tgcagggatt aaaggcgtgt attaccactg tctggcaggt ttcctaatct
     4021 ttttgtgagc taaggaagtg agactggatt ggactcacta cttaggtaga agaatttaaa
     4081 tctagtaaga tctttttgga atacttagta taatgtaaca tttaattagt ggcagcaatt
     4141 tttatactac gaccaataac caatgcccac acccagtcag aaactcacca gcttggagca
     4201 aacttggaag atgctgtgga tgtacccctt gggctatcaa cctttggact aatcctgggt
     4261 gaagctggtg tgcaggtcga acaataggaa catgagggac aaggggaact tggtgggtag
     4321 ggcggagaaa aggtgttcct gggactgggg atgccaaggt aggtgttttc cttcctgctg
     4381 ttttaggtcg ataatcttgt tctttggtat aacggctggt ctgggagagt ggggtggaac
     4441 atgaagaccg ctgtaatgtt gaaagcttag gatttgtcgt agaagagtct tgatcagtat
     4501 tgggtatggg attggatgac agcaggttct ctgtaaggat tcatttacag gacattttga
     4561 tatcctttat caatttattt caattcccaa caacccagca aatcttactt ttaaaaattc
     4621 tgacaattct tttttccttt tttttttttt tggtttttca agacatggtt tctctgtata
     4681 gccctggctg tcctggaact cactttgtag accaggctgg cctcgaactc agaaatccac
     4741 ctgcctatgc ttcccaagtg ctgggattaa aggcgtgtgc caccatgccg gctctttttt
     4801 cctcttaacc atgttagatg ctttcatgga aagtcatcta tttgctttaa atgtagacag
     4861 atgcacagct ggtactctcc ataagccccg gagttgagtt atggtcatag agtagttagg
     4921 ccttgagcta ggaatagatg cccacatttt atttataacc ttattttctt atattatgat
     4981 gtatatggat ggtgttttgc ctgtatgtat gtctgtgtgc ctgtcgagtt cagtgctcac
     5041 agaggccagg ggagggtgct aggtcaccta gggctgaagt cacaaaaggt gagctatcat
     5101 gtgggtgctg gcaattgaac ccaggtcctc tggaagagca aacagtgccc tgatctatct
     5161 ctccagtcca tagatgccaa ggtcttcaaa ttctttgctg aaataattat ttttgatgtt
     5221 gtcatttcct ttcttgggga gtgaacatga acccatggtc ttgtacatgc tggcaagtat
     5281 tctactactt agtacaattg agctgcattc ccggtgcata aaggaataac tcaaaaacct
     5341 ctgacatagt gttacaaaac aaaaagcaca agttgccagg catgtagttg aggcaccttt
     5401 aatcctaaga cttgggaggc agagaccagt agatctctgt gagtttgagg ttagccacag
     5461 ctacacagta agatcctgtt gcaaataaaa caaacaacct gtaacagaaa caacaacaac
     5521 cctgcatcta tccccctatc tacctggata ctcttgaaga ggacctcttc ccagcaccca
     5581 catgtggctc acaattctag aggttctgat gccctcttct ggcctccttg ggcactggat
     5641 acacatgatg cagacatacc acaggcaaat acacaaatac gttaaaaaaa gaattgagct
     5701 atatcaatat gctaaatgag tttgttattc aacttgaaag atggtaagaa tcataagcta
     5761 aagcacatcg ttaccccggc tggccacaat gtcaagtgag caagtttgag ctcactaacc
     5821 catctccctg acactgcagt cactcatgcc tatgttctga cacaggtaag cagcagtacc
     5881 ttcgctggtc ttctgagtat cctctttacc atcaccagga accaccattc caggtgccat
     5941 ttcctccttg gttttctctc tgctttcata catcttccgg atcactgagg taggggtgaa
     6001 ggaaggagaa agctgcagag aagaggcaga gatcagcaca ctcaagtgcc tttgcctaac
     6061 actaagccag tgctttatct tttcaggaca ctgggaatta agcctagggt cttacatatg
     6121 ctttggagca cttgaccact gggctatgca gcactcgctc ttaagcctag ctttaccagg
     6181 tgaagccatc ttcaacaggg taaagagttc ccattttgtt tacttgggca gaggtaattc
     6241 taatgttctg acctatagct actacaagag aacacaaagg ggcaagtagt cattgtccca
     6301 agaacttcaa tccatctttg ccaccctgta aaatgggcct gtgtgtgttc tcatgtttct
     6361 caaccctgaa atagggctaa gaagagggaa gagcatggct ggaaatatac cttagtaatg
     6421 gggaagagat gtttcagaga cctcagaacc tatgattcca cttgttcctt gtatttttct
     6481 tttaaaatta catacatgaa aactcaatta tcgtacacct tactagaact taccattaag
     6541 gatcctttgt tcttgtttat tttataatga aattatataa ttttataatg aattttataa
     6601 aggatgagac agggcctaac tcatgaacaa aattccttct ggagtttctc taaagcaaga
     6661 gcctttggga tatttagtgc agggtttcat atggagtcct gaagcacaga actattctag
     6721 tccccttgtc tatcagtttt gattggataa gtctgtatca gttaaaaaca aaaacaaaaa
     6781 tcccaaccca ctggtaaagc tagatgtatc atcacaaata aacccataag agaacaattt
     6841 acagacctgc cagcatgttc actggactcc catcatgtga ccagtcattc ctctctcatc
     6901 tcctgcctca catttgtcct tctactttcc cacaacagct aatcttgttt tattttttgg
     6961 agggtcacag taggccctca caaggcccca ggctggcctt aaattctatt tgctcgactg
     7021 aattggttcc tttaaagcct gcttttttgt acaaacttgt tgattcgagg tgatggtgac
     7081 gatgaattcg agctcggtac ccggggatct tccgggcagg ctcgcgttcc gaggccggcc
     7141 gagcagatag gtctttggga tgtaaagcag ctgcccgggg tcccgaggaa actcggccgt
     7201 ggtgaccaac acgaaacaaa agcaaacgcg gccgctagcg tcgcatcgat ttcgctacct
     7261 taggaccgtt atagttagga tctgccggtc tccctatagt gagtcgtatt aatttcgata
     7321 agccaggttg ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag
     7381 tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga
     7441 tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
     7501 cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt
     7561 aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca
     7621 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
     7681 accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     7741 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
     7801 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc
     7861 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca
     7921 ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta
     7981 aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt
     8041 caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt
     8101 ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaaggg
     8161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8221 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8281 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8341 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8401 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8461 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8581 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     8641 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8701 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8761 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8821 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8881 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     8941 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9001 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9061 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9181 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9241 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9301 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     9361 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     9421 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     9481 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     9541 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     9601 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     9661 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     9721 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttt cgcgagctcg
     9781 acctgcagcc aagcttgggt cgtggacctg accccacgca acgcccaaaa taataacccc
     9841 cacgaaccat aaaccattcc ccatggggga ccccgtccct aacccacggg gccagtggct
     9901 atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg
     9961 gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg tctcggtggg
    10021 gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca
    10081 cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
    10141 ctccttccgt gtttcagtta gcctccccca tctcccgggc aaacgtgcgc gcaattaacc
    10201 ctcactaaag ggaacaaaag ctgggtaccg ggccccccct cgagccccag ctggttcttt
    10261 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
    10321 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
    10381 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
    10441 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
    10501 aggctgatca gcgagctcta ggatctgcat tccaccactg ctcccattca tcagttccat
    10561 aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta
    10621 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
    10681 caccacagaa gtaaggttcc ttcacaaaga gatcgcctga cacgatttcc tgcacaggct
    10741 tgagccatat actcatacat cgcatcttgg ccacgttttc cacgggtttc aaaattaatc
    10801 tcaagttcta cgcttaacgc tttcgcctgt tcccagttat taatatattc aacgctagaa
    10861 ctcccctcag cgaagggaag gctgagcact acacgcgaag caccatcacc gaaccttttg
    10921 ataaactctt ccgttccgac ttgctccatc aacggttcag tgagacttaa acctaactct
    10981 ttcttaatag tttcggcatt atccactttt agtgcgagaa ccttcgtcag tcctggatac
    11041 gtcactttga ccacgcctcc agcttttcca gagagcgggt tttcattatc tacagagtat
    11101 cccgcagcgt cgtatttatt gtcggtacta taaaaccctt tccaatcatc gtcataattt
    11161 ccttgtgtac cagattttgg cttttgtata cctttttgaa tggaatctac ataaccaggt
    11221 ttagtcccgt ggtacgaaga aaagttttcc atcacaaaag atttagaaga atcaacaaca
    11281 tcatcaggat ccatggcgag gacctgcagg tcgaaaggcc cggagatgag gaagaggaga
    11341 acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc
    11401 gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca ccccttccca
    11461 gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc caaaggccta
    11521 cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga cgtgctactt
    11581 ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc ctgactaggg
    11641 gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg cgcctaccgg
    11701 tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg
    11761 ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc cggtagaatt
    11821 cgccaatgac aagacgctgg gcggggtttg ctcgacattg ggtggaaaca ttccaggcct
    11881 gggtggagag gctttttgct tcctcttgca aaaccacact gctcgacatt gggtggaaac
    11941 attccaggcc tgggtggaga ggctttttgc ttcctcttga aaaccacact gctcgatttg
    12001 ttagcagcct cgaatcaacc actttgtaca agaaagctgg gtctagatat ctccagttgg
    12061 tttaaactaa ttggctgttg aatgacagcg atcccatgac tcagtgtgca acactgcttt
    12121 agcaagtggc tttgactgtt tgtttattct gatgtattta gaagagggct tttatctgtt
    12181 gtagttctgt gccttttaag atccaggatg ctaaatttga taagctggca cctgggcagt
    12241 tagggaagca gctgcttgct acagccactg actctttatt cctctccata cagtgctttg
    12301 gtctggggtc tgtctgtgaa acataatcct cagaaagtgg gtaaagacca cacattggaa
    12361 gatgaagacg tcattcagat tgtgaagaag tgaagtcctc ttgccatctg ctggctggcc
    12421 acagcagtgt tccttatgac taagtacctt cctcagcccc tctgtctttg gcagcctctg
    12481 gattgggata cagggattga tatggagact tatcaaactg aaacaccaca atttttttct
    12541 ttccggagat agggtgttat gtatttcagg ctggctttga actcttgcag ctgaggctgg
    12601 cctagaatcc ctttcatcag cctccatctc ctcagtgctg ggcttaccac tgtgcacctt
    12661 tagctcctca gcaacaccac tttccctact ttgctgtcac ttctattgaa ctgtataaag
    12721 aagctggtgg gcccgctggc catgtgtcgt tattcatgtg aggtttgctg tgtgatgagg
    12781 tgaatgaggc aacgcctgct tggtcatagg aaatgggaca gagaatggga cctgtgtccg
    12841 tttgtccatt ggtagtgtgc tgtaatgata ggctttcacc tgggcttggc tttctgtcct
    12901 ttcaagtggt ccttctcagg acattggaga gaggaaggta taataggtaa agttaactgc
    12961 agatttttct ccagcgcaat agtctctgga ttctgagctg agcggactgc tgtgatgagg
    13021 aggtattttc ttcatttata tttccaatgc tactctgaaa gtctcccaac ccccctcccc
    13081 tcgctttctt ggccctggtg ttcccttgta ctgaggcaca taaagtttgt aagaccaatg
    13141 ggcctctctt tccactgatg gccatctagg ccatcttctg ctatatatcc agctagagac
    13201 acgagctccg gggggtactg gttagttcat actgttgttc cacctatagg gtgaggtgca
    13261 agttgtctat tagccctttc tgataccctc ttttgctgtc tgaagttata tatatatata
    13321 tatatataaa cactacagag aaaccctgtc tcaggaaaaa aacaaaaaac aaaacaaaaa
    13381 caaaaacagg ctgagtgaca agaaccacca tacagtgaat atttcctacg actgacactg
    13441 tgctaagcag cttccaaaac cctcccctta ataccagcaa tcccaacctt aaaagttaat
    13501 gccactgttc catgtacgac acgatgagat tgaggctcac aaatacagta atcttttttt
    13561 tttttttttt tttttttttt tggtttttcg agacagggtt tctctgtata gctctggctg
    13621 tcctggaact cactttgtag accaggctgg cctcaaactc agagatccgg cctgcctctg
    13681 cctcccaagt gctgggatta aaggtgtgtg ccaccaccgc ctggctggaa gattatttta
    13741 atgtggtctg cagtgatgat agaggaggcc ttgagaaaga caggaacgtc tttgtaggag
    13801 aacagacttt gcttcttctg tcttcaaagg agatgagcta gagccttcag aggtctgagg
    13861 aaagctgtgg gccttaccac acactaccct tccctcaggg ctccgtctgc tgccctagag
    13921 ctagctctct tcctttctcc gggtttaaaa ctgtccttgg aatcaggcct ctcaagcctc
    13981 aaaatataga ctggtttctc actaggacat tgactgatag gctatgactg aaagcctgtc
    14041 cccacactaa agaattggtt tccactgggc cggagagcac tggttaagag cactgactgc
    14101 tcttctgaag gtcctgagtt aaaatcccag caaccacaag gtagctcaca gccatccgtc
    14161 atgagatctg ggatgtctga agacagctac agtgtactta cttataaata aataaatggt
    14221 taaaaaaaaa aagaattggc ttatcagaac tgtggaattt ccagctaggt ttcttctcca
    14281 gtgagagcct agatttttgg aagtcctcag gccaagcaaa aatttacttt gctttagctt
    14341 tgtacttcag gatgctgaaa agagaaggaa gttcaaatcc tgtacaaggc ctcagtggtt
    14401 ttgaagaggc cttgagtcca ttttttagta cccctctaca ctcctcccac ccagttgcta
    14461 gcccaacagg atttgagaag ctggtgtgac aaatacctat attggcattt ggaaggtaga
    14521 ggcaagagtg ttaggagttc aaggctcacc ttgacttgaa atgctttctc cagccaaaca
    14581 tacatgaatt tacctttgct cttggggctt agacttgatg gttctgaatg aagatagagt
    14641 ttgtatcaga tacactagct gggaatgtgc tgcccgttgt tgggatctgc ggcaagtgtt
    14701 ctggcctctt cccccccaaa ttgaaacgaa gacctcacat ggatttgtac tagaagtaag
    14761 gtttggagca aaacagtagt gtagcttatg gcaggaaaca ctatcagaga ttgactgcag
    14821 gctgagtgag tgggggtacg taaggaccca aagctatgta gcctagcttc tcaaggtgtc
    14881 taaagaaagg aagggtagat taccaatgag cagagtttgg gcttctctat ttttaaagtt
    14941 ttaaaatgtt tatatcaagg caaggtttct gttgtccttg ctggtctgga actagattgg
    15001 ccttgaccta agagatgatg cgacttctgc ctcccaagtg ctaattaaag gtgtgagtca
    15061 ccacgcttgc cttggtgttt tctatttata ggttatatat tcatttttcc tattgcatgt
    15121 cacttcccaa attacatctc atctagtcat ctgtgcaccc aatcacaaat aggcataacc
    15181 tggtagattt ccactaaaag ttacttagag gaagtttgtg aatttattat tacaggcaag
    15241 ccatggaaat gtgtgtagct gttgcagttg gtttgaggag gtgtaagtgc atggtgtatt
    15301 tggtagagag actaggctgc ctagttcatt atgagaagat atgtacaaca caaacgaagt
    15361 agggtctcag gttcaactgt taagctttct tgcctcaggc tgctggagag ttgcactcgt
    15421 acttattcac tcaattcatt ctttggccag gtgggtggtg gtggtggtgg ttcacacctt
    15481 tagtcatagt gttgaggagg cagaggcaag aggttctgtg tttgaggcca gctgggtcta
    15541 tagattgaat tctaggaaag ccagggctgc atagagaaac ctgttttcaa aagaaaaaag
    15601 aaaaaaagaa aagacgaatg gtgtatgtgt gtgtttgtca gtcatctgtc tggaattgaa
    15661 tttgagatcc ccctgcctcc caagtgtcct gattaaaggc atagtctata tagctgggag
    15721 tggtggcgca cacttttaat tctagcactt gagaggcaga ggcaggcgga tctctgagtt
    15781 cgaggcc
//
