LOCUS       sequence-247152-       11719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A self-assembling split-GFP based Golden Gate vector to determine 
            protein targeting to plastids in plant cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB
  TITLE     Targeting specificity of nuclear-encoded organelle proteins with a 
            self-assembling split-fluorescent protein toolkit.
  JOURNAL   J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 
            10.1242/jcs.230839.
  PUBMED    31085714
REFERENCE   2  (bases 1 to 11719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-06-3"; volume: "132(11). pii: jcs.230839. doi"; 
            pages: " 10.1242/jcs.230839"
COMMENT     Sequence Label: pLaNGo11-7
FEATURES             Location/Qualifiers
     source          1..11719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1030..1375
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1634..2275
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     terminator      2306..3013
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        4030..4375
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(4505..4810)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4607..4626)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5130..5813)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     5728..5747
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5814..5916)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5947..6372
                     /codon_start=1
                     /product="seven tandem copies of the 11th beta-strand of 
                     superfolder GFP"
                     /label=GFP11x7
                     /note="each GFP11 associates with GFP(1-10), consisting of 
                     the first 10 beta-strands, to reconstitute fluorescent GFP 
                     (Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDH
                     MVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSG
                     GRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGIT"
     misc_feature    7077..7101
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7381..8010
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8439..9512
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9578..9772
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9834..9938
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9939..10733
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10904..11492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11393..11412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11628..11652
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgatgt atcacctctc gcaagaattc aagcttggag gaattccaat cccacaaaaa
       61 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
      121 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
      181 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
      241 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
      301 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
      361 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
      421 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
      481 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
      541 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
      601 gcaagtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
      661 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
      721 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
      781 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
      841 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tcaagactgg
      901 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
      961 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca
     1021 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     1081 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     1141 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     1201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac
     1261 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     1321 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
     1381 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
     1441 aattacattt acaattatcg ataccatgac caccgctgtc accgccgctg tttctttccc
     1501 ctctaccaaa accacctctc tctccgcccg aagctcctcc gtcatttccc ctgacaaaat
     1561 cagctacaaa aaggttcctt tgtactacag gaatgtatct gcaactggga aaatgggacc
     1621 catcagggcc gcaatgtcca aaggagaaga actgtttacc ggtgttgtgc caattttggt
     1681 tgaactcgat ggtgatgtca acggacataa gttctcagtg agaggcgaag gagaaggtga
     1741 cgccaccatt ggaaaattga ctcttaaatt catctgtact actggtaaac ttcctgtacc
     1801 atggccgact ctcgtaacaa cgcttacgta cggagttcag tgcttttcga gatacccaga
     1861 ccatatgaaa agacatgact tttttaagtc ggctatgcct gaaggttacg tgcaagaaag
     1921 aacaatttcg ttcaaagatg atggaaaata taaaactaga gcagttgtta aatttgaagg
     1981 agatactttg gttaaccgca ttgaactgaa aggaacagat tttaaagaag atggtaatat
     2041 tcttggacac aaactcgaat acaattttaa tagtcataac gtatacatca ctgctgataa
     2101 gcaaaagaac ggaattaaag cgaatttcac agtacgccat aatgtagaag atggcagtgt
     2161 tcaacttgcc gaccattacc aacaaaacac ccctattgga gacggtccgg tacttcttcc
     2221 tgataatcac tacctctcaa cacaaacagt cctgagcaaa gatccaaatg aaaaaggaac
     2281 aggtggcggc ggaagttagc ttgtcctgct ttaatgagat atgcgagaag cctatgatcg
     2341 catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc
     2401 agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt
     2461 tttatgaata atattctccg ttcaatttac tgattgtacc ctactactta tatgtacaat
     2521 attaaaatga aaacaatata ttgtgctgaa taggtttata gcgacatcta tgatagagcg
     2581 ccacaataac aaacaattgc gttttattat tacaaatcca attttaaaaa aagcggcaga
     2641 accggtcaaa cctaaaagac tgattacata aatcttattc aaatttcaaa agtgccccag
     2701 gggctagtat ctacgacaca ccgagcggcg aactaataac gctcactgaa gggaactccg
     2761 gttccccgcc ggcgcgcatg ggtgagattc cttgaagttg agtattggcc gtccgctcta
     2821 ccgaaagtta cgggcaccat tcaacccggt ccagcacggc ggccgggtaa ccgacttgct
     2881 gccccgagaa ttatgcagca tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca
     2941 aaccttgaca gtgacgacaa atcgttgggc gggtccaggg cgaattttgc gacaacatgt
     3001 cgaggctcag caggaccgct actagaattc gagctcggag gaattccaat cccacaaaaa
     3061 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
     3121 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
     3181 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
     3241 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
     3301 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
     3361 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
     3421 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
     3481 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
     3541 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
     3601 gcaagtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
     3661 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
     3721 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
     3781 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
     3841 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tcaagactgg
     3901 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
     3961 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca
     4021 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     4081 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     4141 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     4201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac
     4261 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     4321 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
     4381 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
     4441 aattacattt acaattatcg atacaatgtg agaccgactg gctgtgtata agggagcctg
     4501 acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc
     4561 tgagatcagc cacttcttcc ccgataacgg aaaccggcac actggccata tcggtggtca
     4621 tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat
     4681 ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccaggc gtgtcaataa
     4741 tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
     4801 taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat ttcaataaac
     4861 cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga
     4921 cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct
     4981 tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc
     5041 tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc
     5101 gcgcaaatac gcatactgtt atctggcttt tagtaagccg gatccacgcg gcgtttacgc
     5161 cccccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     5221 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     5281 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     5341 aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata
     5401 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     5461 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     5521 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     5581 ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     5641 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     5701 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     5761 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     5821 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     5881 ttatggtgaa agttggaacc tcttacgtgc cgatcaaggt ctcattcgat gggcggcaaa
     5941 ttcatgcgtg accacatggt ccttcatgag tatgtaaatg ctgctgggat tacaggtggc
     6001 tctggaggta gagatcatat ggttctccac gaatacgtta acgccgcagg catcactggc
     6061 ggtagtggag gacgcgacca tatggtacta catgaatatg tcaatgcagc cggaataacc
     6121 ggagggtccg gaggccggga tcacatggtg ctgcatgagt atgtgaacgc ggcgggtata
     6181 actggtgggt cgggcggacg agatcatatg gtgcttcacg aatacgtaaa cgcagctggc
     6241 attactggcg gatcaggtgg cagggatcac atggtactcc atgagtacgt gaacgctgct
     6301 ggaatcacag gcggtagcgg cggtcgggac catatggtcc tgcacgaata tgtcaatgct
     6361 gccggtatca cctaagctta gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca
     6421 agttcaatgc atcagtttca ttgcgcacac accagaatcc tactgagttt gagtattatg
     6481 gcattgggaa aactgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt
     6541 attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat
     6601 atggtccttt tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg
     6661 ttgaatttga aattataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat
     6721 cgtggcctct aatgaccgaa gttaatatga ggagtaaaac acttgtagtt gtaccattat
     6781 gcttattcac taggcaacaa atatattttc agacctagaa aagctgcaaa tgttactgaa
     6841 tacaagtatg tcctcttgtg ttttagacat ttatgaactt tcctttatgt aattttccag
     6901 aatccttgtc agattctaat cattgcttta taattatagt tatactcatg gatttgtagt
     6961 tgagtatgaa aatatttttt aatgcatttt atgacttgcc aattgattga caacgcttta
     7021 cgaggatgca catgtgaccg agggacacga agtgatccgt ttaaactatc agtgtttgac
     7081 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     7141 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgccagccg tgcggctgca
     7201 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     7261 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     7321 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     7381 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     7441 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     7501 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     7561 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     7621 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     7681 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     7741 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     7801 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     7861 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     7921 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     7981 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     8041 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     8101 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     8161 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     8221 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     8281 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     8341 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc
     8401 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     8461 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     8521 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     8581 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg tcgtcgatta
     8641 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     8701 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     8761 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     8821 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     8881 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     8941 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     9001 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     9061 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     9121 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     9181 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     9241 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     9301 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     9361 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     9421 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     9481 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     9541 ccctagcagg ggaaaaaggt cgaaaaagct tctttcctgt ggatagcacg tacattggga
     9601 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     9661 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     9721 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     9781 gccagcgcac agccgaacag ctgcaaaaag cgcccacatc aaggctccga gtgcgcggaa
     9841 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     9901 cctgataaat gcttcaataa tattgaaaaa ggaagagtat ggctaaaatg agaatatcac
     9961 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
    10021 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
    10081 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
    10141 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
    10201 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
    10261 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
    10321 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
    10381 ataacgatct ggccgatgtg gattgcgaaa actgggaaga ggacactcca tttaaagatc
    10441 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
    10501 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
    10561 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcgc
    10621 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
    10681 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagctgtcag
    10741 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    10801 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    10861 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    10921 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    10981 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    11041 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    11101 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    11161 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    11221 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    11281 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    11341 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    11401 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    11461 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    11521 tcctgctcgg atctgttgga ccggacagta gtcatggttg atgggctgcc tgtatcgagt
    11581 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
    11641 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
    11701 tactggggtt gaacactct
//
