LOCUS       sequence-247190-       15256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15256)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX113
FEATURES             Location/Qualifiers
     source          1..15256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5370..5394)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5423..5512
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5546..5798
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6227..6479)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6947..7972)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6947..7972)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7982..8326)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8468..8485)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8468..8484)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8477..8499)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8624..8643)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8834..8855
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9102..9126
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10722..11870)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12169..12963)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13155..13922
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14615..15246
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgctcta
     4621 ttttttctct ataaatggtg gttcactagt tctttttcaa ctccgatcct tgcccataat
     4681 aagagaggtg aaaatggtga gacaaaagat tcagatcaag aagattgaca acttgacggc
     4741 aaggcaagtg actttctcaa agagaagaag agggcttttc aagaaagctc aggagctgtc
     4801 aacactttgt gatgctgata ttggcctaat cgttttctct gctactggaa aactttttga
     4861 atattcaagc tccagtatga tgcaactgat tgaaaagcat aaggtgcact cagaaaggga
     4921 tatggatagt ccagaccaac tgcaatcttt taactttcag agcgcaaaga aaaactatgg
     4981 aatgcttagt tgggaatttg cggagaagaa ccgggagttg aggcaactta atggagaaga
     5041 gctgcaagga cttggcttgg aggaattaat gaaattggag aaaatagtcg agggaggaat
     5101 aagtcgtgtc atgaaaatta agggtgaaaa gtttatgaca gagatcagct ccctcaagaa
     5161 aaaggaagct caactgcaag aagagaattc acagttgaaa cagcaatcac aacaggcaag
     5221 attgaatgaa gttgagggcc ataatgcaat tgagggggga aactcggcag actctatcac
     5281 gactaatcgc agcttagtca atggtcatca cgattacatt gactcagata ctagcctcaa
     5341 gttaggcttg ccttttcctt accccgggga cccagctttc ttgtacaaag tggttgataa
     5401 cagcgggtta attaacatct tttacccata cgatgttcct gactatgcgg gctatcccta
     5461 tgacgtcccg gactatgcag gatcctatcc atatgacgtt ccagattacg ctgctcagtg
     5521 cagcgcttag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
     5581 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     5641 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     5701 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     5761 taaattatcg cgcgcggtgt catctatgtt actagatcga tcaaacttcg gtactgtgta
     5821 atgacgatga gcaatcgaga ggctgactaa caaaaggtat gcccaaaaac aacctctcca
     5881 aactgtttcg aattggaagt ttctgctcat gccgacaggc ataacttaga tattcgcggg
     5941 ctattcccac taattcgtcc tgctggtttg cgccaagata aatcagtgca tctccttaca
     6001 agttcctctg tcttgtgaaa tgaactgctg actgcccccc aagaaagcct cctcatctcc
     6061 cagttggcgg cggctgatac accatcgaaa acccacgtcc gaacacttga tacatgtgcc
     6121 tgagaaatag gcctacgtcc aagagcaagt cctttctgtg ctcgtcggaa attcctctcc
     6181 tgtcagacgg tcgtgcgcat gtcttgcgtt gatgaagcta attcccgatc tagtaacata
     6241 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
     6301 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
     6361 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
     6421 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
     6481 gcttcgacgc actccttctt tactccacca tctcgtcctt attgaaaacg tgggtagcac
     6541 caaaacgaat caagtcgctg gaactgaagt taccaatcac gctggatgat ttgccagttg
     6601 gattaatctt gcctttcccc gcatgaataa tattgatgaa tgcatgcgtg aggggtattt
     6661 cgattttggc aatagctgca attgccgcga catcctccaa cgagcataat tcttcagaaa
     6721 aatagcgatg ttccatgttg tcagggcatg catgatgcac gttatgaggt gacggtgcta
     6781 ggcagtattc cctcaaagtt tcatagtcag tatcatattc atcattgcat tcctgcaaga
     6841 gagaattgag acgcaatcca cacgctgcgg caaccttccg gcgttcgtgg tctatttgct
     6901 cttggacgtt gcaaacgtaa gtgttggatc ccggtcggca tctactctat tcctttgccc
     6961 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
     7021 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
     7081 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
     7141 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc
     7201 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
     7261 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
     7321 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
     7381 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
     7441 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
     7501 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
     7561 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg
     7621 cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
     7681 caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa
     7741 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     7801 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     7861 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
     7921 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atatcggggt
     7981 cgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
     8041 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
     8101 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     8161 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     8221 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     8281 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     8341 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     8401 gacggatctc taggacgcgt cgacgcgtcg acgcgtcgac gcgtcgacgc gtcctagaag
     8461 ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     8521 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     8581 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt cgctttcttc
     8641 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8701 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     8761 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8821 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     8881 tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa caggattttc
     8941 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     9001 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccacccca gtacattaaa
     9061 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     9121 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     9181 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     9241 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     9301 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     9361 atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc atttctcgct
     9421 taaccgtgac aggctgtcga tcttgagaac tatgccgaca taataggaaa tcgctggata
     9481 aagccgctga ggaagctgag tggcgctatt tctttagaag tgaacgttga cgatatcaac
     9541 tcccctatcc attgctcacc gaatggtaca ggtcggggac ccgaagttcc gactgtcggc
     9601 ctgatgcatc cccggctgat cgaccccaga tctggggctg agaaagccca gtaaggaaac
     9661 aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta aacccgctcc
     9721 gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg ggattggcgg
     9781 atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt gccaggcggt
     9841 aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc cgaacgccat
     9901 ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg cgtttggtgt
     9961 caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg
    10021 tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct gcacagcgcc
    10081 taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca agctccagaa
    10141 tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc ccgttggaat
    10201 ctgtcggacg atcatcacga gcaataaacc ckccggcaac gcccgcagca gcataccggc
    10261 gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg ccttgtgagc
    10321 gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc cgtcgatgta
    10381 ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc
    10441 ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg acaatcggat
    10501 ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct taatcacaat
    10561 tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga
    10621 tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg
    10681 ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc
    10741 aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct
    10801 tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg
    10861 aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt
    10921 cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca
    10981 gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca
    11041 cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg
    11101 gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa
    11161 taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc
    11221 ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca
    11281 tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa
    11341 atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca
    11401 gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac
    11461 aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg
    11521 gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata
    11581 gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga
    11641 cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg
    11701 tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg
    11761 cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc
    11821 gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct
    11881 tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct
    11941 ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc
    12001 tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc
    12061 ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag
    12121 tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggtact aaaacaattc
    12181 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
    12241 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
    12301 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
    12361 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
    12421 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
    12481 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
    12541 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
    12601 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
    12661 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
    12721 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
    12781 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
    12841 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
    12901 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
    12961 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
    13021 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
    13081 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
    13141 aaaccacaat tatgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga
    13201 ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg
    13261 gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca ctgccgtaaa
    13321 acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt
    13381 gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg cgttggcctc
    13441 aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc
    13501 cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat gtcggggcta
    13561 aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg gttgttgcgc
    13621 acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct
    13681 ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa
    13741 agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga
    13801 ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc
    13861 atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt ggagtcatta
    13921 cccaattatg atagaattta caagctataa ggttattgtc ctgggtttca agcattagtc
    13981 catgcaagtt tttatgcttt gcccattcta tagatatatt gataagcgcg ctgcctatgc
    14041 cttgccccct gaaatcctta catacggcga tatcttctat ataaaagata tattatctta
    14101 tcagtattgt caatatattc aaggcaatct gcctcctcat cctcttcatc ctcttcgtct
    14161 tggtagcttt ttaaatatgg cgcttcatag agtaattctg taaaggtcca attctcgttt
    14221 tcatacctcg gtataatctt acctatcacc tcaaatggtt cgctgggttt atcgcacccc
    14281 cgaacacgag cacggcaccc gcgaccacta tgccaagaat gcccaaggta aaaattgccg
    14341 gccccgccat gaagtccgtg aatgccccga cggccgaagt gaagggcagg ccgccaccca
    14401 ggccgccgcc ctcactgccc ggcacctggt cgctgaatgt cgatgccagc acctgcggca
    14461 cgtcaatgct tccgggcgtc gcgctcgggc tgatcgccca tcccgttact gccccgatcc
    14521 cggcaatggc aaggactgcc agcgctgcca tttttggggt gaggccgttc gcggccgagg
    14581 ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg
    14641 ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca
    14701 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac
    14761 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat
    14821 aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat
    14881 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc
    14941 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc
    15001 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt
    15061 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc
    15121 gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct
    15181 ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggtg
    15241 agcgtcggaa aggcgc
//
