LOCUS       sequence-247192-       16527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16527)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX114
FEATURES             Location/Qualifiers
     source          1..16527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6641..6665)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6694..6783
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6817..7069
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7498..7750)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8218..9243)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8218..9243)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9253..9597)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9739..9756)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9739..9755)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9748..9770)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9895..9914)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10105..10126
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10373..10397
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11993..13141)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13440..14234)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14426..15193
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15886..16517
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggctccacca ctagtgtgtt ccctcttaat tctctctctc tcttgactct
     5881 ctttctgtct atttttggaa agaaaagcaa aagtagcaat aattaacaac aatggctaga
     5941 gagaaaattc agatcaagaa aattgataac gcaacagcaa gacaagtaac cttctcaaag
     6001 agaagaagag ggcttttcaa gaaagctgaa gaactttctg ttctttgtga tgctgatatt
     6061 gctctcatta ttttctcatc tactggaaaa ctctttgagt attctagctc cagcatgaag
     6121 gaaattcttg agaggcgtga tttgcattca aagaatttgg aaaaattaga ccaaccatca
     6181 cttgagctgc agctagtaga gaacagcaat tactccagac taagcaagga aatatctgag
     6241 aaaagtcatc gattaagaca aatgagagga gaagaactcc aaggattaag tattgaagaa
     6301 ttgcagcagt tggagagatc tctcgaagtt ggattgacac gtgtcataga gagaaagggt
     6361 gataaaataa tgacagagat caaccagctt cagcaaaagg gcatgcaact aatggaagag
     6421 aatgaaagat tgaagcaaca ggtgatggag atatccaata atggatacaa caacccagca
     6481 gcagtattag tagcagttga atcagaaaat gggtatatta acgaggaagg acaatcatct
     6541 gaatctgtca ctaatgcatg taactctact ggccctcctc aggatgatga cagttctgat
     6601 acttctctca agttggggct gccctactca ggtcccgggg acccagcttt cttgtacaaa
     6661 gtggttgata acagcgggtt aattaacatc ttttacccat acgatgttcc tgactatgcg
     6721 ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt tccagattac
     6781 gctgctcagt gcagcgctta gagctcgaat ttccccgatc gttcaaacat ttggcaataa
     6841 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     6901 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     6961 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     7021 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattagct
     7081 tcatcaacgc aagacatgcg cacgaccgtc tgacaggaga ggaatttccg acgagcacag
     7141 aaaggacttg ctcttggacg taggcctatt tctcaggcac atgtatcaag tgttcggacg
     7201 tgggttttcg atggtgtatc agccgccgcc aactgggaga tgaggaggct ttcttggggg
     7261 gcagtcagca gttcatttca caagacagag gaacttgtaa ggagatgcac tgatttatct
     7321 tggcgcaaac cagcaggacg aattagtggg aatagcccgc gaatatctaa gttatgcctg
     7381 tcggcatgag cagaaacttc caattcgaaa cagtttggag aggttgtttt tgggcatacc
     7441 ttttgttagt cagcctctcg attgctcatc gtcattacac agtaccgaag tttgatcgat
     7501 ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt
     7561 tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa
     7621 ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata
     7681 tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt
     7741 ttgaacgatc tgcttcgacg cactccttct ttactccacc atctcgtcct tattgaaaac
     7801 gtgggtagca ccaaaacgaa tcaagtcgct ggaactgaag ttaccaatca cgctggatga
     7861 tttgccagtt ggattaatct tgcctttccc cgcatgaata atattgatga atgcatgcgt
     7921 gaggggtatt tcgattttgg caatagctgc aattgccgcg acatcctcca acgagcataa
     7981 ttcttcagaa aaatagcgat gttccatgtt gtcagggcat gcatgatgca cgttatgagg
     8041 tgacggtgct aggcagtatt ccctcaaagt ttcatagtca gtatcatatt catcattgca
     8101 ttcctgcaag agagaattga gacgcaatcc acacgctgcg gcaaccttcc ggcgttcgtg
     8161 gtctatttgc tcttggacgt tgcaaacgta agtgttggat cccggtcggc atctactcta
     8221 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
     8281 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
     8341 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
     8401 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
     8461 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
     8521 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
     8581 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
     8641 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
     8701 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
     8761 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
     8821 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
     8881 cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc
     8941 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc
     9001 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
     9061 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
     9121 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac
     9181 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt
     9241 catatcgggg tcgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc
     9301 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
     9361 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
     9421 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
     9481 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
     9541 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
     9601 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
     9661 tccaccatgt tgacggatct ctaggacgcg tcgacgcgtc gacgcgtcga cgcgtcgacg
     9721 cgtcctagaa gctaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     9781 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     9841 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg cccgctcctt
     9901 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     9961 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    10021 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    10081 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    10141 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa
    10201 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
    10261 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccacccc
    10321 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
    10381 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
    10441 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
    10501 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
    10561 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
    10621 tgctgcctgt gatcaaatat catctccctc gcagagatcc gaattatcag ccttcttatt
    10681 catttctcgc ttaaccgtga caggctgtcg atcttgagaa ctatgccgac ataataggaa
    10741 atcgctggat aaagccgctg aggaagctga gtggcgctat ttctttagaa gtgaacgttg
    10801 acgatatcaa ctcccctatc cattgctcac cgaatggtac aggtcgggga cccgaagttc
    10861 cgactgtcgg cctgatgcat ccccggctga tcgaccccag atctggggct gagaaagccc
    10921 agtaaggaaa caactgtagg ttcgagtcgc gagatccccc ggaaccaaag gaagtaggtt
    10981 aaacccgctc cgatcaggcc gagccacgcc aggccgagaa cattggttcc tgtaggcatc
    11041 gggattggcg gatcaaacac taaagctact ggaacgagca gaagtcctcc ggccgccagt
    11101 tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc acttgcgggt cagcacggtt
    11161 ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga cgccgacagg atctagcgct
    11221 gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc cgcaaatagc ccccaggacc
    11281 gccatcaatc gtatcgggct acctagcaga gcggcagaga tgaacacgac catcagcggc
    11341 tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc ggtagaccga aataaacaac
    11401 aagctccaga atagcgaaat attaagtgcg ccgaggatga agatgcgcat ccaccagatt
    11461 cccgttggaa tctgtcggac gatcatcacg agcaataaac ccgccggcaa cgcccgcagc
    11521 agcataccgg cgacccctcg gcctcgctgt tcgggctcca cgaaaacgcc ggacagatgc
    11581 gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga ccgacagccc aatgatctcg
    11641 ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc gttcagcgaa cgcctccatg
    11701 ggctttttct cctcgtgctc gtaaacggac ccgaacatct ctggagcttt cttcagggcc
    11761 gacaatcgga tctcgcggaa atcctgcacg tcggccgctc caagccgtcg aatctgagcc
    11821 ttaatcacaa ttgtcaattt taatcctctg tttatcggca gttcgtagag cgcgccgtgc
    11881 gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt tcctgaaatg
    11941 ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg ccctagcgtt
    12001 tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg cctcgcaact
    12061 cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc
    12121 acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt
    12181 cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg aatttcgtgt
    12241 agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg caacgggacg
    12301 ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc
    12361 caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg
    12421 ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg
    12481 tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc tgctgccaca
    12541 ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc acgtccttgt
    12601 tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg
    12661 tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg
    12721 caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg
    12781 cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt tttcgcttct
    12841 tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct
    12901 gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca gggggagcca
    12961 gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag ccgacggact
    13021 ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg
    13081 cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac gtccgattca
    13141 ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct tattcctgat
    13201 ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg aagtcggtcc
    13261 cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc tgcacgaata
    13321 ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc ctgagagcca aaacacttga
    13381 tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac atctaggtac
    13441 taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc
    13501 agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg
    13561 acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag
    13621 ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag
    13681 acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta
    13741 aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag
    13801 taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg
    13861 atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt
    13921 tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc
    13981 cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat
    14041 agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc
    14101 atttttaaat ataggttttc attttctccc accagcttat ataccttagc aggagacatt
    14161 ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt
    14221 ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag
    14281 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
    14341 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
    14401 agccgatttt gaaaccacaa ttatgggtga tgctgccaac ttactgattt agtgtatgat
    14461 ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta
    14521 ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat ctctgctctc
    14581 actgccgtaa aacatggcaa ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc
    14641 agaaaatcat tgatatggcc atgaatggcg ttggatgccg ggcaacagcc cgcattatgg
    14701 gcgttggcct caacacgatt ttacgtcact taaaaaactc aggccgcagt cggtaacctc
    14761 gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac agtggggcta
    14821 tgtcggggct aaatcgcgcc agcgctggct gttttacgcg tatgacagtc tccggaagac
    14881 ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct
    14941 gctgtcaccc tttgacgtgg tgatatggat gacggatggc tggccgctgt atgaatcccg
    15001 cctgaaggga aagctgcacg taatcagcaa gcgatatacg cagcgaattg agcggcataa
    15061 cctgaatctg aggcagcacc tggcacggct gggacggaag tcgctgtcgt tctcaaaatc
    15121 ggtggagctg catgacaaag tcatcgggca ttatctgaac ataaaacact atcaataagt
    15181 tggagtcatt acccaattat gatagaattt acaagctata aggttattgt cctgggtttc
    15241 aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat tgataagcgc
    15301 gctgcctatg ccttgccccc tgaaatcctt acatacggcg atatcttcta tataaaagat
    15361 atattatctt atcagtattg tcaatatatt caaggcaatc tgcctcctca tcctcttcat
    15421 cctcttcgtc ttggtagctt tttaaatatg gcgcttcata gagtaattct gtaaaggtcc
    15481 aattctcgtt ttcatacctc ggtataatct tacctatcac ctcaaatggt tcgctgggtt
    15541 tatcgcaccc ccgaacacga gcacggcacc cgcgaccact atgccaagaa tgcccaaggt
    15601 aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg acggccgaag tgaagggcag
    15661 gccgccaccc aggccgccgc cctcactgcc cggcacctgg tcgctgaatg tcgatgccag
    15721 cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg ctgatcgccc atcccgttac
    15781 tgccccgatc ccggcaatgg caaggactgc cagcgctgcc atttttgggg tgaggccgtt
    15841 cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg gccgggaggg
    15901 ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct
    15961 ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt
    16021 ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct
    16081 caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc
    16141 gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc
    16201 ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt
    16261 gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg
    16321 ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg
    16381 ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg
    16441 acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa
    16501 ccagcccggt gagcgtcgga aaggcgc
//
