LOCUS       sequence-247200-       11609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast plasmid overexpressing LovB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang
            Y, Cai M
  TITLE     Engineered ethanol-driven biosynthetic system for improving 
            production of acetyl-CoA derived drugs in Crabtree-negative yeast.
  JOURNAL   Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. 
            Epub 2019 May 9.
  PUBMED    31077813
REFERENCE   2  (bases 1 to 11609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-07"; volume: "54:275-284. doi"; pages: " 
            10.1016/j.ymben.2019.05.001. Epub 2019 May 9"
COMMENT     Sequence Label: pZ_lacO-cP-AOX1-LovB
FEATURES             Location/Qualifiers
     source          1..11609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    105..121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             9424..9441
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      9521..9767
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        9782..10195
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        10202..10249
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10268..10642
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      10708..10955
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(10724..10742)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(join(11030..11609,1..9))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11110..11129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
       61 tcacgttaag ggattttggt catgagatca gatcttgtgt ggaattgtga gcggataaca
      121 atttcacaca ctaaccccta cttgacagca atatataaac agaaggaagc tgccctgtct
      181 taaacctttt tttttatcat cattattagc ttactttcat aattgcgact ggttccaatt
      241 gacaagcttt tgattttaac gacttttaac gacaacttga gaagatcaaa aaacaactaa
      301 ttattcgaaa tggctcaatc tatgtatcct aatgagccta ttgtcgtggt cggcagtggt
      361 tgtcgcttcc ctggtgacgc caacacaccc tccaagctct gggagctact ccagcatcct
      421 cgcgatgtgc agagtcgaat ccccaaagaa cgatttgacg tcgacacatt ttatcacccg
      481 gacgggaagc accacgggcg aacaaatgca ccctacgcct atgttctcca agacgatctg
      541 ggcgccttcg atgcggcctt cttcaatatc caggctggag aggccgagag tatggacccc
      601 cagcaccggc tgttgctgga gacggtgtac gaggccgtaa cgaatgctgg aatgcgtatc
      661 caggatctgc agggaacttc gactgctgtt tacgtcgggg tgatgacgca cgactatgag
      721 actgtctcaa cccgcgacct ggagagcatc cccacctact cggcgacggg tgtcgcggtc
      781 agtgttgcgt ccaaccgcat ctcgtatttt tttgactggc atggaccaag tatgacgatc
      841 gatacggcat gcagctcgtc gttggttgcc gttcatctgg cggtgcaaca gctacggacg
      901 ggtcaaagct ccatggcaat tgctgcgggt gcgaatctga ttctggggcc catgacattc
      961 gtccttgaaa gcaaattgag catgctatcc ccctcgggtc gatcccgcat gtgggacgcc
     1021 ggagctgacg gctatgccag aggcgaagct gtttgctctg tagtgttgaa gacattgagt
     1081 caagccttgc gcgatgggga cacgattgaa tgtgtcatcc gagaaactgg ggtgaatcaa
     1141 gatggccgaa cgaccggaat tacgatgccg aaccatagtg ctcaggaggc actcatcaag
     1201 gctacctacg cccaggctgg ccttgacatc accaaggccg aggacaggtg ccaattcttc
     1261 gaggctcatg ggactggtac tccggccgga gatccccagg aggcggaggc cattgcaaca
     1321 gccttcttcg gccacgagca ggtagcacgc agcgacggaa acgagagggc ccctctgttc
     1381 gtgggcagtg cgaaaactgt tgtcgggcac accgagggca cggccggtct ggctggtctc
     1441 atgaaggcgt cgttcgctgt ccgccatggg gtaatccccc ccaacctgct gttcgacaaa
     1501 atcagcccgc gagtcgcccc attctataaa aacctgagga ttccgacaga agctacccaa
     1561 tggccagctc tcccacccgg acaaccgcgc cgcgccagtg tcaactcctt tggattcggc
     1621 ggcacgaatg cgcatgccat tattgaggaa tacatggagc cagagcaaaa ccagctgcga
     1681 gtctcgaata atgaggactg cccacccatg accggtgtcc tgagtttacc cttagtcctc
     1741 tcggcgaagt cccagcgctc cttaaagata atgatggagg agatgctgca attccttgag
     1801 tctcaccccg agatacactt gcacgacctc acctggtcct tactgcgcaa gcggtcagtt
     1861 ctacccttcc gccgggctat tgtcggccat agtcatgaaa ccatccgccg ggctttggag
     1921 gatgccatcg aggatggtat tgtgtcgagc gacttcacta cggaggtcag aggccagcca
     1981 tcggtgttgg gaatcttcac cgggcagggg gcgcagtggc cggggatgtt aaagaatctg
     2041 atagaggcat cgccatatgt gcggaacata gtgagggagc tggacgactc cctgcagagc
     2101 ttgccggaaa aataccggcc ctcgtggacg ctactggacc agttcatgct agaaggagag
     2161 gcctccaacg tccaatatgc tactttctcc cagccattat gctgcgcggt gcaaattgtc
     2221 ctggtccgtc tccttgaagc cgcgagaata cgattcacgg ctgttgttgg acatagctcc
     2281 ggcgaaattg cttgcgcctt tgctgccggg ctcatcagtg cctcgttggc gattcggatt
     2341 gcttacttac gtggagtcgt ctcggcaggg ggcgccagag gcacaccggg agccatgttg
     2401 gccgccggga tgtcctttga ggaagcacaa gagatctgcg agttggatgc ctttgagggc
     2461 cgcatctgcg tggctgccag caattcccca gacagtgtaa ctttctctgg cgacgcgaac
     2521 gcaattgatc acctgaaggg catgttggag gatgagtcca cttttgcgag actgctcaag
     2581 gtcgatacag cgtaccactc gcatcatatg cttccatgtg cagacccata tatgcaagcc
     2641 ctagaagagt gtggttgtgc tgttgccgat gcaggttccc cagccggaag tgtaccctgg
     2701 tattcgtccg tggacgccga gaacaggcaa atggcagcaa gagacgtgac cgccaagtac
     2761 tggaaagata acttagtatc tccggtgcta ttctcccacg cagtgcagcg ggcagtcgtc
     2821 acgcacaagg cgctggatat cgggattgaa gtgggctgtc acccagctct caagagccca
     2881 tgcgtcgcca ccatcaagga tgtcctatct ggggttgacc tggcgtatac aggttgcttg
     2941 gagcgaggaa agaatgatct cgattcattc tctcgagcac tggcatatct ctgggaaagg
     3001 tttggtgcct ccagtttcga tgcggacgag ttcatgcgtg cagtcgcgcc tgatcggccc
     3061 tgtatgagtg tgtcgaagct cctaccggcc tatccatggg accgctctcg tcgctactgg
     3121 gtggaatccc gagcaactcg ccaccatctt cgagggccca agccccatct tctattagga
     3181 aagctctccg aatacagcac tccgctaagc ttccagtggc tgaattttgt gcgcccacga
     3241 gacattgaat ggcttgatgg acatgcattg caaggccaga ctgtcttccc tgcggccggc
     3301 tatatcgtca tggcaatgga agcagcctta atgattgctg gcacccacgc aaagcaggtc
     3361 aagttactgg agatcttgga tatgagcatt gacaaggcgg tgatatttga cgacgaagac
     3421 agcttggttg agctcaacct gacagctgac gtgtctcgca acgccggcga agcaggttca
     3481 atgaccataa gcttcaagat cgattcctgt ctatcgaagg agggtaacct atccctatca
     3541 gccaagggcc aactggccct aacgatagaa gatgtcaatc ccaggacgac ttccgctagc
     3601 gaccagcacc atcttccccc gccagaagag gaacatcctc atatgaaccg tgtcaacatc
     3661 aatgctttct accacgagct ggggttgatg gggtacaact acagtaagga cttccggcgt
     3721 ctccataaca tgcaacgagc agatcttcga gccagcggca ccttagactt cattcctctg
     3781 atggacgagg gtaatggctg tcctctcctg ctgcatcctg catcattgga cgtcgccttc
     3841 cagactgtca tcggcgcata ctcctcccca ggtgatcggc gtctacgctg tctgtatgta
     3901 cccactcacg ttgatcgcat cacacttgtc ccatcccttt gcctggcaac ggctgagtcc
     3961 ggatgcgaga aggttgcctt caatactatc aatacgtacg acaagggaga ctacttgagc
     4021 ggtgacattg tggtgtttga cgcggagcag accaccctgt tccaggttga aaatattact
     4081 tttaagccct tttcaccccc ggatgcttca actgaccatg cgatgtttgc ccgatggagc
     4141 tggggtccgt tgactccgga ctcgctgctg gataacccgg agtattgggc caccgcgcag
     4201 gacaaggagg cgattcctat tatcgaacgc atcgtctact tctatatccg atcgttcctc
     4261 agtcagctta cgctggagga gcgccagcag gcagccttcc atttgcagaa gcagatcgag
     4321 tggctcgaac aagtcctggc cagcgccaag gagggtcgtc acctatggta cgaccccggg
     4381 tgggagaatg atactgaggc ccagattgag cacctttgta ctgctaactc ctaccaccct
     4441 catgttcgcc tggttcagcg agtcggccaa cacctgctcc ccaccgtacg atcgaacggc
     4501 aacccattcg accttctgga ccacgatggg ctcctgacgg agttctatac caacacactc
     4561 agcttcggac ccgcactaca ctacgcccgg gaattggtgg cgcagatcgc ccatcgctat
     4621 cagtcaatgg atattctgga gattggagca gggaccggcg gcgctaccaa gtacgtgttg
     4681 gccacgcccc agctggggtt caacagctac acatacaccg atatctccac cggattcttc
     4741 gagcaagcgc gggagcaatt tgcccccttc gaggaccgga tggtgtttga acccctcgat
     4801 atccgccgca gtcccgccga gcagggcttc gagccgcatg cctatgatct gatcattgcc
     4861 tccaatgtgc tacatgcgac acccgaccta gagaaaacca tggctcacgc ccgctctctg
     4921 ctcaagcctg gaggccagat ggttattctg gagattaccc acaaagaaca cacacggctc
     4981 gggtttatct ttggtctgtt cgccgactgg tgggctgggg tggatgatgg tcgctgcact
     5041 gagccgtttg tctcgttcga ccgctgggat gcgatcctaa agcgtgtcgg gttttccggt
     5101 gtggacagtc gcaccacgga tcgggacgca aatctattcc cgacctctgt gtttagtacc
     5161 catgcaattg acgccaccgt ggagtactta gacgcgccgc ttgccagcag cggcaccgtc
     5221 aaggactctt accctccctt ggtggtggta ggagggcaga ccccccaatc tcagcgtctc
     5281 ctgaacgata taaaagcgat catgcctcct cgtccgctcc agacatacaa gcgcctcgtg
     5341 gatttgctag acgcggagga gctgccgatg aagtccacgt ttgtcatgct cacggagctg
     5401 gacgaggaat tattcgccgg gctcactgaa gagaccttcg aggcaaccaa gctgctgctc
     5461 acgtacgcca gcaatacggt ctggctgaca gaaaatgcct gggtccaaca tcctcaccag
     5521 gcgagcacga tcggcatgct acgctccatc cgccgggagc atcctgactt gggagttcat
     5581 gttctggacg tcgacgcggt tgaaaccttc gatgcaacct tcctggttga acaggtgctt
     5641 cggcttgagg agcatacgga tgagctggcc agttcaacta catggactca agaacccgag
     5701 gtctcctggt gtaaaggccg cccgtggatt cctcgtctga agcgcgatct ggctcgcaat
     5761 aaccgaatga actcctcgcg ccgtcccata tacgagatga tcgattcgtc gcgggctccc
     5821 gtggcattac agacggctcg ggattcatca tcctacttct tggagtccgc tgaaacctgg
     5881 tttgtgcctg agagtgttca gcagatggaa acaaagacga tctatgtcca ctttagctgt
     5941 ccccatgcgc ttagggtcgg acagctcggg tttttctatc ttgtgcaggg tcacgtccag
     6001 gagggcaatc gcgaagtgcc cgtcgtggcc ttagcagagc gtaacgcatc cattgtgcac
     6061 gttcgtcccg attatatata tactgaggca gataacaatc tgtctgaggg tggtggcagc
     6121 cttatggtaa ccgtcctcgc cgcggcggtg ttggcggaga cggtgatcag taccgccaag
     6181 tgcctggggg taactgactc aatcctcgtt ctgaatcccc ccagcatatg tgggcagatg
     6241 ttgctccatg ctggtgaaga gatcggtctt caagttcatc tggccaccac ttctggcaac
     6301 aggagttcgg tttctgctgg agacgccaag tcctggctaa cattgcatgc tcgcgacacg
     6361 gactggcacc tgcgacgggt actgccccgg ggtgtccagg ctttagtcga cttatcagcc
     6421 gaccagagct gtgaaggttt gactcagagg atgatgaaag ttctgatgcc tggctgtgcc
     6481 cattaccgtg cggcagacct gttcacagac accgtttcca ctgaattgca tagcggatcg
     6541 cggcatcaag cttcactgcc cgccgcatat tgggagcatg tggtatcctt agcccgccag
     6601 ggacttccta gtgtcagcga ggggtgggag gtgatgccgt gcactcaatt tgcagcgcat
     6661 gccgacaaga cgcgcccgga tctctcgaca gttatttcct ggccccggga gtcggacgag
     6721 gctacgcttc ctaccagggt tcgctccatt gacgctgaga ccctctttgc ggccgacaaa
     6781 acatatctcc tggtcggact gactggagat cttggacgat cactaggtcg ttggatggtc
     6841 cagcatgggg cctgccacat tgtacttacg agcagaaatc cgcaggtgaa ccccaagtgg
     6901 ctggcgcatg ttgaagaact gggtggtcga gtcactgttc tttccatgga cgtgacaagc
     6961 caaaactcag tggaagctgg cctggctaaa ctcaaggatc tgcatctgcc accagtgggg
     7021 ggtattgcct ttggccctct ggttctgcag gatgtgatgc taaataatat ggaactgcca
     7081 atgatggaga tggtgctcaa ccccaaggtc gaaggcgtcc gcatcctgca cgagaagttc
     7141 tccgatccga ccagtagcaa ccctctcgac ttcttcgtga tgttctcctc gattgtggcc
     7201 gtcatgggca acccgggtca ggctaactac agtgcggcta actgctacct tcaagcgctg
     7261 gcgcagcagc gagttgcatc cggattagca gcgtccacca tcgacatcgg tgccgtgtac
     7321 ggcgttgggt tcgtcactcg ggcggagctg gaggaggact ttaatgcaat tcggttcatg
     7381 ttcgattcgg ttgaggaaca tgaactgcat acactgtttg ctgaggcagt ggtggccggt
     7441 cgacgagccg tgcaccagca agagcagcag cggaagttcg cgacagtgct cgacatggct
     7501 gatctggaac tgacaaccgg aattccgccc ctggaaccag ccctcaaaga tcggatcacc
     7561 ttcttcgacg acccccgcat aggcaactta aaaattccgg agtaccgagg ggccaaagca
     7621 ggcgaagggg cagccggctc caagggctcg gtcaaagaac agctcttgca ggcgacgaac
     7681 ctggaccagg tccgtcagat cgtcatcgat ggactctccg cgaagctgca ggtgaccctg
     7741 cagatccccg atggggaaag cgtgcatccc accatcccac taatcgatca gggggtggac
     7801 tctctgggcg cggtcaccgt gggaacctgg ttctccaagc agctgtacct tgatttgcca
     7861 ctcctgaaag tgcttggggg tgcttcgatc accgatctcg ctaatgaggc tgctgcgcga
     7921 ttgccaccta gctccattcc cctcgtcgca gccaccgacg ggggtgcaga gagcactgac
     7981 aatacttccg agaatgaagt ttcgggacgc gaggatactg accttagtgc cgccgccacc
     8041 atcactgagc cctcgtctgc cgacgaagac gatacggagc cgggcgacga ggacgtcccg
     8101 cgttcccacc atccactgtc tctcgggcaa gaatactcct ggagaatcca gcagggagcc
     8161 gaagacccca ccgtctttaa caacaccatt ggtatgttca tgaagggctc tattgacctt
     8221 aaacggctgt acaaggcgtt gagagcggtc ttgcgccgcc acgagatctt ccgcacgggg
     8281 tttgccaacg tggatgagaa cgggatggcc cagctggtgt ttggtcaaac caaaaacaaa
     8341 gtccagacca tccaagtgtc tgaccgagcc ggcgccgaag agggctaccg acaactggtg
     8401 cagacacggt ataaccctgc cgcaggagac accttgcggc tggtggactt cttctggggc
     8461 caggacgacc atctgctggt tgtggcttac caccgactcg tcggggatgg atctactaca
     8521 gagaacatct tcgtcgaagc gggccagctc tacgacggca cgtcgctaag tccacatgtc
     8581 cctcagtttg cggacctggc ggcacggcaa cgcgcaatgc tcgaggatgg gagaatggag
     8641 gaggatctcg cgtactggaa gaaaatgcat taccgaccgt cctcaattcc agtgctccca
     8701 ctgatgcggc ccctggtagg taacagtagc aggtccgata ctccaaattt ccagcactgt
     8761 ggaccctggc agcagcacga agccgtggcg cgacttgatc cgatggtggc cttccgcatc
     8821 aaggagcgca gtcgcaagca caaggcgacg ccgatgcagt tctatctggc ggcgtatcag
     8881 gtgctgttgg cgcgcctcac cgacagcacc gatctcaccg tgggcctcgc cgacaccaac
     8941 cgtgcgactg tcgacgagat ggcggccatg gggttcttcg ccaacctcct tcccctgcgc
     9001 ttccgggatt tccgccccca tataacgttt ggcgagcacc ttatcgccac ccgtgacctg
     9061 gtgcgtgagg ccttgcagca cgcccgcgtg ccctacggcg tcctcctcga tcaactgggg
     9121 ctggaggtcc cggtcccgac cagcaatcaa cctgcgcctt tgttccaggc cgtcttcgat
     9181 tacaagcagg gccaggcgga aagtggaacg attgggggtg ccaagataac cgaggtgatt
     9241 gccacgcgcg agcgcacccc ttacgatgtc gtgctggaga tgtcggatga tcccaccaag
     9301 gaaccgctgc tcacggccaa gttacagagt tcccgctacg aggctcacca ccctcaagcc
     9361 ttcttggaga gctacatgtc ccttctctct atgttctcga tgaatcccgc cctgaagctg
     9421 gcacatcatc atcatcatca ttgagtttgt agccttagac atgactgttc ctcagttcaa
     9481 gttgggcact tacgagaaga ccggtcttgc tagattctaa tcaagaggat gtcagaatgc
     9541 catttgcctg agagatgcag gcttcatttt tgatactttt ttatttgtaa cctatatagt
     9601 ataggatttt ttttgtcatt ttgtttcttc tcgtacgagc ttgctcctga tcagcctatc
     9661 tcgcagctga tgaatatctt gtggtagggg tttgggaaaa tcattcgagt ttgatgtttt
     9721 tcttggtatt tcccactcct cttcagagta cagaagatta agtgagacct tcgtttgtgc
     9781 ggatccccca cacaccatag cttcaaaatg tttctactcc ttttttactc ttccagattt
     9841 tctcggactc cgcgcatcgc cgtaccactt caaaacaccc aagcacagca tactaaattt
     9901 cccctctttc ttcctctagg gtgtcgttaa ttacccgtac taaaggtttg gaaaagaaaa
     9961 aagagaccgc ctcgtttctt tttcttcgtc gaaaaaggca ataaaaattt ttatcacgtt
    10021 tctttttctt gaaaattttt ttttttgatt tttttctctt tcgatgacct cccattgata
    10081 tttaagttaa taaacggtct tcaatttctc aagtttcagt ttcatttttc ttgttctatt
    10141 acaacttttt ttacttcttg ctcattagaa agaaagcata gcaatctaat ctaagggcgg
    10201 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
    10261 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
    10321 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
    10381 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
    10441 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
    10501 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
    10561 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
    10621 cgtggccgag gagcaggact gacacgtccg acgcggcccg acgggtccga ggcctcggag
    10681 atccgtcccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac
    10741 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
    10801 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
    10861 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
    10921 agaaggtttt gggacgctcg aaggctttaa tttgcaagct ggagaccaac atgtgagcaa
    10981 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    11041 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    11101 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    11161 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    11221 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    11281 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    11341 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    11401 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    11461 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    11521 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    11581 gcaagcagca gattacgcgc agaaaaaaa
//
