LOCUS       sequence-247201-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescnece reporter of beta-Actin with Skylan-S. Express in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Yi X, Son S, Ando R, Miyawaki A, Weiss S
  TITLE     Moments reconstruction and local dynamic range compression of high 
            order superresolution optical fluctuation imaging.
  JOURNAL   Biomed Opt Express. 2019 Apr 16;10(5):2430-2445. doi: 
            10.1364/BOE.10.002430. eCollection 2019 May 1.
  PUBMED    31149378
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1364/BOE.10.002430"; journalName: "Biomed Opt Express"; date: 
            "2019-04-16- 16"; volume: "10"; issue: "5"; pages: "2430-2445"
COMMENT     Sequence Label: Skylan-S-(GGGGS)x3-beta-Actin
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..681
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1..681
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.1
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 atgagtgcga ttaagccaga catgaagatc aaactccgta tggaaggcaa cgtaaacggg
       61 caccactttg tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg
      121 gatcttgaag tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca
      181 ttctcctacg gcaacagggt attcgccaaa tatccagaca acatacaaga ctattttaag
      241 cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt
      301 tgcaacgcca gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt
      361 tatggtacca actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag
      421 ccctccactg agaaaatgta tgtgcgtgat ggagtgctga cgggtgatgt agagatggct
      481 ttgttgcttg aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag
      541 gagaagggtg tcaagttacc aggcgcccac tttgtggacc actgcattga gattttaagc
      601 catgacaaag attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg
      661 cctgacaatg ccagacgata a
//
