LOCUS       sequence-247205-        3584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast plasmid overexpressing LovC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang
            Y, Cai M
  TITLE     Engineered ethanol-driven biosynthetic system for improving 
            production of acetyl-CoA derived drugs in Crabtree-negative yeast.
  JOURNAL   Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. 
            Epub 2019 May 9.
  PUBMED    31077813
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-07"; volume: "54:275-284. doi"; pages: " 
            10.1016/j.ymben.2019.05.001. Epub 2019 May 9"
COMMENT     Sequence Label: pZ_lacO-cP-AOX1-LovC
FEATURES             Location/Qualifiers
     source          1..3584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    64..80
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1358..1375
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1455..1701
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1716..2129
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2136..2183
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2202..2576
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      2642..2889
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2658..2676)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(2964..3552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3044..3063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagatcag atcttgtgtg
       61 gaattgtgag cggataacaa tttcacacac taacccctac ttgacagcaa tatataaaca
      121 gaaggaagct gccctgtctt aaaccttttt ttttatcatc attattagct tactttcata
      181 attgcgactg gttccaattg acaagctttt gattttaacg acttttaacg acaacttgag
      241 aagatcaaaa aacaactaat tattcgaaat gggcgaccag ccattcattc caccaccgca
      301 gcaaacagcg ctgacggtaa atgaccatga tgaagtcacc gtctggaatg ccgcaccctg
      361 ccccatgctg ccccgcgacc aggtatacgt ccgcgtcgag gccgtggcga tcaatcccag
      421 tgacacgaag atgcgcggac agtttgccac gccctgggcg tttctcggaa cggactatgc
      481 cggcacggtc gtcgcagtgg gttcggacgt gactcatatc caagtgggtg accgggtcta
      541 cggggcacag aacgagatgt gcccacgcac cccggatcag ggggcattct cgcagtacac
      601 ggtcacgcga ggccgtgttt gggccaagat ccccaagggc ttgtcgttcg agcaggctgc
      661 cgcgctacct gcgggcatca gtaccgctgg attggcgatg aagttgcttg ggctgccttt
      721 gccatcgcct tcggcagacc agccacccac ccactccaag ccggtgtatg tgttggtcta
      781 tgggggcagt acggccactg ccactgtcac tatgcaaatg ctccgcctgt ccggatatat
      841 tccaattgca acatgctccc cccacaattt cgacctggcc aaatcgcgcg gcgcagagga
      901 ggtctttgac tatcgggccc cgaatctcgc gcagacgatc cgtacctaca ccaagaacaa
      961 tctccgctat gctctcgact gtatcaccaa cgtcgagtcc accacattct gcttcgcagc
     1021 catcggccgc gcgggggggc actacgtctc cctgaacccg ttccctgaac acgcggccac
     1081 gcgcaagatg gtcacgaccg actggaccct ggggccgacc atctttggcg agggatcaac
     1141 ctggcccgcc ccctatgggc gtcccggcag tgaggaagag cggcagttcg gcgaggatct
     1201 gtggcgcatc gcggggcagc tcgtcgaaga tggacgcctc gtccatcatc cgttgcgcgt
     1261 ggtgcagggc ggcttcgatc acattaagca aggcatggag ctcgtccgga agggagagct
     1321 gtcgggggag aaactcgtgg ttcggctcga ggggccgcat catcatcatc atcattgagt
     1381 ttgtagcctt agacatgact gttcctcagt tcaagttggg cacttacgag aagaccggtc
     1441 ttgctagatt ctaatcaaga ggatgtcaga atgccatttg cctgagagat gcaggcttca
     1501 tttttgatac ttttttattt gtaacctata tagtatagga ttttttttgt cattttgttt
     1561 cttctcgtac gagcttgctc ctgatcagcc tatctcgcag ctgatgaata tcttgtggta
     1621 ggggtttggg aaaatcattc gagtttgatg tttttcttgg tatttcccac tcctcttcag
     1681 agtacagaag attaagtgag accttcgttt gtgcggatcc cccacacacc atagcttcaa
     1741 aatgtttcta ctcctttttt actcttccag attttctcgg actccgcgca tcgccgtacc
     1801 acttcaaaac acccaagcac agcatactaa atttcccctc tttcttcctc tagggtgtcg
     1861 ttaattaccc gtactaaagg tttggaaaag aaaaaagaga ccgcctcgtt tctttttctt
     1921 cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt tcttgaaaat tttttttttt
     1981 gatttttttc tctttcgatg acctcccatt gatatttaag ttaataaacg gtcttcaatt
     2041 tctcaagttt cagtttcatt tttcttgttc tattacaact ttttttactt cttgctcatt
     2101 agaaagaaag catagcaatc taatctaagg gcggtgttga caattaatca tcggcatagt
     2161 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     2221 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     2281 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     2341 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     2401 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     2461 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     2521 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     2581 tccgacgcgg cccgacgggt ccgaggcctc ggagatccgt cccccttttc ctttgtcgat
     2641 atcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc
     2701 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg
     2761 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg
     2821 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct
     2881 ttaatttgca agctggagac caacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2941 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3001 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3061 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3121 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3181 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3241 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3301 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3361 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3421 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3481 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3541 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctga
//
