LOCUS       sequence-247233-        9981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Buganim Y, Itskovich E, Hu YC, Cheng AW, Ganz K, Sarkar S, Fu D, 
            Welstead GG, Page DC, Jaenisch R
  TITLE     Direct Reprogramming of Fibroblasts into Embryonic Sertoli-like 
            Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2012 Sep 7;11(3):373-86.
  PUBMED    22958931
REFERENCE   2  (bases 1 to 9981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2012-09-7"; volume: "11(3)"; pages: "373-86"
COMMENT     Sequence Label: FUW-TetO-Sox9
FEATURES             Location/Qualifiers
     source          1..9981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1780..1798
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1822..1840
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1864..1882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1906..1924
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1948..1966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1990..2008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2041..2079
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2076..2100
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3839..4427
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3892..3912)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4310..4321)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4430..4446)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4431..4447)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4952..5132
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5158..5175)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5164..5388
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5434..5862
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5521..5540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5731..5752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5871..5891)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5876..6205
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6056..6191
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6118..6137
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6253..6300
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6319..6693
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6823..6944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6860..6879)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6914..6933
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6993..7009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6993..7009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7006..7028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7017..7033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7041..7047,7048..7065,7066..7071))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7086..7107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7224..7241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7395..7983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7475..7494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8154..8945,8946..9014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8777..8796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9015..9119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9194..9213)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9385..9764
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9766..9964
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt tagatctcga gtttaccact ccctatcagt gatagagaaa
     1801 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     1861 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     1921 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     1981 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagctcggta cccgggtcga
     2041 ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
     2101 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
     2161 ctccgcggcc ccgaattcgc ccttatgaat ctcctggacc ccttcatgaa gatgaccgac
     2221 gagcaggaga agggcctgtc tggcgccccc agccccacca tgtcggagga ctcggctggt
     2281 tcgccctgtc cctcgggctc cggctcggac acggagaaca cccggcccca ggagaacacc
     2341 ttccccaagg gcgagccgga tctgaagaag gagagcgagg aagataagtt ccccgtgtgc
     2401 atccgcgagg cggtcagcca ggtgctgaag ggctacgact ggacgctggt gcccatgccc
     2461 gtgcgcgtca acggctccag caagaacaag ccacacgtca agcgacccat gaacgccttc
     2521 atggtgtggg cgcaggctgc gcgcaggaag ctggcagacc agtacccgca tctgcacaac
     2581 gcggagctca gcaagactct gggcaagctc tggaggctgc tgaacgagag cgagaagaga
     2641 cccttcgtgg aggaggcgga gcggctgcgc gtgcagcaca agaaagacca ccccgattac
     2701 aagtaccagc cccggcggag gaagtcggtg aagaacggac aagcggaggc cgaagaggcc
     2761 acggaacaga ctcacatctc tcctaatgct atcttcaagg cgctgcaagc cgactcccca
     2821 cattcctcct ccggcatgag tgaggtgcac tccccgggcg agcactctgg gcaatctcag
     2881 ggtccgccga ccccacccac cactcccaaa accgacgtgc aagctggcaa agttgatctg
     2941 aagcgagagg ggcgccctct ggcagagggg ggcaggcagc cccccatcga cttccgcgac
     3001 gtggacatcg gtgaactgag cagcgacgtc atctccaaca ttgagacctt cgacgtcaat
     3061 gagtttgacc aatacttgcc acccaacggc cacccagggg ttccggccac ccacggccag
     3121 gtcacctaca ctggcagtta cggcatcagc agcaccgcac ccacccctgc gaccgcgggc
     3181 cacgtgtgga tgtcgaagca gcaggcgccg ccccctcctc cgcagcagcc tccgcaggcc
     3241 ccgcaagccc cacaggcgcc tccgcagcag caagcacccc cgcagcagcc gcaggcaccc
     3301 cagcagcagc aggcacacac gctcaccacg ctgagcagcg agccaggcca gtcccagcga
     3361 acgcacatca agacggagca gctgagcccc agccactaca gcgagcagca gcagcactcc
     3421 ccgcaacaga tctcctacag ccccttcaac cttcctcact acagcccctc ctacccgccc
     3481 atcacccgct cgcaatacga ctacgctgac catcagaact ccggctccta ctacagtcac
     3541 gcagccggcc agggctcagg gctctactcc accttcactt acatgaaccc cgcgcagcgc
     3601 cccatgtaca cccccatcgc tgacacctcc ggggtccctt ccatcccgca gacccacagc
     3661 ccgcagcact gggaacaacc agtctacaca cagctcacca gaccctgaga agagaaaagc
     3721 tatggtgaca gagctgatct tttttttttt tttttttaaa gaagaaaaga aagaaacgaa
     3781 aagaaaaagc tgaagaaatc aagaaccaaa gggcgaattc gatatcaagc ttatcgataa
     3841 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3901 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3961 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4021 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4081 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4141 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4201 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4261 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4321 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4381 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     4441 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     4501 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     4561 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     4621 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     4681 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     4741 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     4801 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     4861 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     4921 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     4981 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5041 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5101 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     5161 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5221 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5281 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5341 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     5401 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     5461 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5521 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5581 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5641 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5701 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5761 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5821 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     5881 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5941 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6001 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6061 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6121 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     6181 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     6241 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     6301 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     6361 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     6421 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     6481 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     6541 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     6601 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     6661 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     6721 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6781 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6841 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6901 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6961 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7021 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7081 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7141 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7201 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7261 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7321 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7381 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7441 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7501 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7561 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7621 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7681 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7741 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7801 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7861 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7921 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7981 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8041 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8101 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8161 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8221 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8281 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8341 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8401 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8461 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8521 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8581 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8641 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8701 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8761 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8821 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8881 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8941 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9001 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9061 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9121 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     9181 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     9241 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     9301 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     9361 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     9421 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     9481 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     9541 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     9601 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     9661 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     9721 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     9781 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     9841 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     9901 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     9961 gcagcgcgtt ttgcctgtac t
//
