LOCUS       sequence-247256-       15504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15504)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX117
FEATURES             Location/Qualifiers
     source          1..15504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5618..5642)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5671..5760
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5794..6046
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6475..6727)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7195..8220)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7195..8220)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8230..8574)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8716..8733)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8716..8732)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8725..8747)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8872..8891)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9082..9103
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9350..9374
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10970..12118)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12417..13211)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13403..14170
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14863..15494
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtgtc tcttaccagt ttctatggcg
     4621 gcgattacac aacgaaaaga atcggatcgg attaagggtc catggagtcc tgaagaggac
     4681 gagctgttgc agactctagt ggagaaacat ggacccagaa attggtcttt aataagcaaa
     4741 tcggttccgg gtcgatccgg aaaatcctgc aggctccgat ggtgtaacca gctttcccct
     4801 caagtggaac accgtgcttt cactccagag gaggatgaaa ccatcatcag ggcccacgct
     4861 aagttcggta acaaatgggc tactattgcc cgtttactat cgggtcggac cgataacgct
     4921 attaagaacc actggaactc tactcttaaa cgtaagtgtt gttccatgtc tgaagattta
     4981 agctttgaaa ctcctcaaca gcctttgaaa agatcgtcta gtgtcggtcc tagtaacaac
     5041 ttttcttctg gcatgaatcc gggtagccct tccggatccg acttgagtga ttcgattcct
     5101 tcgggtttta cccaatcgct tgtttatagg cctgtccccc gaaccggcgg gattttccct
     5161 ctttccccac cagttcaaca gatcgaaact gctcctttta tacccgaccc acccacttct
     5221 ctttgccttt cgctacccgg atccgtgacc cgtgaaatct cgaatctgac tgcacaacca
     5281 acccagcaaa ctcagccccc tcaatcacca ccactaccac cgcctcctcc tttggctttg
     5341 ccgccggttg ataaactagt accgccacca acagctgctt tcatggctca gcttcctcaa
     5401 actaagcaaa gttatgattt ttgttcacca gctttaccaa tatcaggaga gaaacagttg
     5461 tttactccgg aatttttggg tgttctacaa gatatgataa gaaaggaagt aaggaattac
     5521 atgtctggtg ttgaacacaa tgggatttgc atgcaaactg atgcaataag aaatgcagta
     5581 gttaagcgta ttgggattag caagattgag cccggggacc cagctttctt gtacaaagtg
     5641 gttgataaca gcgggttaat taacatcttt tacccatacg atgttcctga ctatgcgggc
     5701 tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc agattacgct
     5761 gctcagtgca gcgcttagag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     5821 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     5881 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     5941 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     6001 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attagcttca
     6061 tcaacgcaag acatgcgcac gaccgtctga caggagagga atttccgacg agcacagaaa
     6121 ggacttgctc ttggacgtag gcctatttct caggcacatg tatcaagtgt tcggacgtgg
     6181 gttttcgatg gtgtatcagc cgccgccaac tgggagatga ggaggctttc ttggggggca
     6241 gtcagcagtt catttcacaa gacagaggaa cttgtaagga gatgcactga tttatcttgg
     6301 cgcaaaccag caggacgaat tagtgggaat agcccgcgaa tatctaagtt atgcctgtcg
     6361 gcatgagcag aaacttccaa ttcgaaacag tttggagagg ttgtttttgg gcataccttt
     6421 tgttagtcag cctctcgatt gctcatcgtc attacacagt accgaagttt gatcgatcta
     6481 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
     6541 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
     6601 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
     6661 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
     6721 aacgatctgc ttcgacgcac tccttcttta ctccaccatc tcgtccttat tgaaaacgtg
     6781 ggtagcacca aaacgaatca agtcgctgga actgaagtta ccaatcacgc tggatgattt
     6841 gccagttgga ttaatcttgc ctttccccgc atgaataata ttgatgaatg catgcgtgag
     6901 gggtatttcg attttggcaa tagctgcaat tgccgcgaca tcctccaacg agcataattc
     6961 ttcagaaaaa tagcgatgtt ccatgttgtc agggcatgca tgatgcacgt tatgaggtga
     7021 cggtgctagg cagtattccc tcaaagtttc atagtcagta tcatattcat cattgcattc
     7081 ctgcaagaga gaattgagac gcaatccaca cgctgcggca accttccggc gttcgtggtc
     7141 tatttgctct tggacgttgc aaacgtaagt gttggatccc ggtcggcatc tactctattc
     7201 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     7261 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     7321 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     7381 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg
     7441 cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     7501 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     7561 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     7621 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     7681 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     7741 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     7801 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
     7861 atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg
     7921 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc
     7981 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     8041 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     8101 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     8161 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
     8221 atcggggtcg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     8281 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     8341 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     8401 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     8461 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     8521 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     8581 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     8641 accatgttga cggatctcta ggacgcgtcg acgcgtcgac gcgtcgacgc gtcgacgcgt
     8701 cctagaagct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8761 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8821 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgccc gctcctttcg
     8881 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8941 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     9001 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     9061 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     9121 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggaacca ccatcaaaca
     9181 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     9241 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccccagt
     9301 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
     9361 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
     9421 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     9481 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
     9541 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
     9601 tgcctgtgat caaatatcat ctccctcgca gagatccgaa ttatcagcct tcttattcat
     9661 ttctcgctta accgtgacag gctgtcgatc ttgagaacta tgccgacata ataggaaatc
     9721 gctggataaa gccgctgagg aagctgagtg gcgctatttc tttagaagtg aacgttgacg
     9781 atatcaactc ccctatccat tgctcaccga atggtacagg tcggggaccc gaagttccga
     9841 ctgtcggcct gatgcatccc cggctgatcg accccagatc tggggctgag aaagcccagt
     9901 aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa gtaggttaaa
     9961 cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt aggcatcggg
    10021 attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc cgccagttgc
    10081 caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag cacggttccg
    10141 aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg
    10201 tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc caggaccgcc
    10261 atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat cagcggctgc
    10321 acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat aaacaacaag
    10381 ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca ccagattccc
    10441 gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc ccgcagcagc
    10501 ataccggcga cccctcggcc tcgctgttcg ggctccacga aaacgccgga cagatgcgcc
    10561 ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg acagcccaat gatctcgccg
    10621 tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc ctccatgggc
    10681 tttttctcct cgtgctcgta aacggacccg aacatctctg gagctttctt cagggccgac
    10741 aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat ctgagcctta
    10801 atcacaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc
    10861 ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca
    10921 gtaaagcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc
    10981 aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt
    11041 cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca
    11101 tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga
    11161 acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt
    11221 ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt
    11281 tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa
    11341 cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct
    11401 tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga
    11461 aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca
    11521 gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga
    11581 cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga
    11641 acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa
    11701 tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg
    11761 cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg
    11821 tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt
    11881 cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt
    11941 gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg acggactgga
    12001 aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg
    12061 aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc
    12121 accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat tcctgatttg
    12181 acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag tcggtcccgt
    12241 agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc acgaatacca
    12301 gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa cacttgatgc
    12361 ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc taggtactaa
    12421 aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt
    12481 aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg
    12541 cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca
    12601 cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca
    12661 agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat
    12721 ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa
    12781 tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg
    12841 gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca
    12901 tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag
    12961 ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc
    13021 atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt
    13081 tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct
    13141 tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc
    13201 attttagcca tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag
    13261 ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc
    13321 agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc
    13381 cgattttgaa accacaatta tgggtgatgc tgccaactta ctgatttagt gtatgatggt
    13441 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
    13501 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctatctc tgctctcact
    13561 gccgtaaaac atggcaactg cagttcactt acaccgcttc tcaacccggt acgcaccaga
    13621 aaatcattga tatggccatg aatggcgttg gatgccgggc aacagcccgc attatgggcg
    13681 ttggcctcaa cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg
    13741 catacagccg ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt ggggctatgt
    13801 cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat gacagtctcc ggaagacggt
    13861 tgttgcgcac gtattcggtg aacgcactat ggcgacgctg gggcgtctta tgagcctgct
    13921 gtcacccttt gacgtggtga tatggatgac ggatggctgg ccgctgtatg aatcccgcct
    13981 gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc ggcataacct
    14041 gaatctgagg cagcacctgg cacggctggg acggaagtcg ctgtcgttct caaaatcggt
    14101 ggagctgcat gacaaagtca tcgggcatta tctgaacata aaacactatc aataagttgg
    14161 agtcattacc caattatgat agaatttaca agctataagg ttattgtcct gggtttcaag
    14221 cattagtcca tgcaagtttt tatgctttgc ccattctata gatatattga taagcgcgct
    14281 gcctatgcct tgccccctga aatccttaca tacggcgata tcttctatat aaaagatata
    14341 ttatcttatc agtattgtca atatattcaa ggcaatctgc ctcctcatcc tcttcatcct
    14401 cttcgtcttg gtagcttttt aaatatggcg cttcatagag taattctgta aaggtccaat
    14461 tctcgttttc atacctcggt ataatcttac ctatcacctc aaatggttcg ctgggtttat
    14521 cgcacccccg aacacgagca cggcacccgc gaccactatg ccaagaatgc ccaaggtaaa
    14581 aattgccggc cccgccatga agtccgtgaa tgccccgacg gccgaagtga agggcaggcc
    14641 gccacccagg ccgccgccct cactgcccgg cacctggtcg ctgaatgtcg atgccagcac
    14701 ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc ccgttactgc
    14761 cccgatcccg gcaatggcaa ggactgccag cgctgccatt tttggggtga ggccgttcgc
    14821 ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc
    14881 gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt
    14941 taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc
    15001 cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa
    15061 atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg
    15121 cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca
    15181 ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg
    15241 aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg
    15301 cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca
    15361 agttttccgc gaggtatcca caacgccggc ggccgcggtg tctcgcacac ggcttcgacg
    15421 gcgtttctgg cgcgtttgca gggccataga cggccgccag cccagcggcg agggcaacca
    15481 gcccggtgag cgtcggaaag gcgc
//
