LOCUS       sequence-24728-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Kwon Y, Hofmann T, Montell C
  TITLE     Integration of phosphoinositide- and calmodulin-mediated regulation 
            of TRPC6.
  JOURNAL   Mol Cell. 2007 Feb 23. 25(4):491-503.
  PUBMED    17317623
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2007 Feb 23. 25(4):491-503."
COMMENT     Sequence Label: pcDNA3-AKT-PH[R25C]-GFP
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      567..576
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(618..639)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cgnggnnntt agcgagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag cttgcggccg cgaattcgcc accatgagcg
      121 acgtggctat tgtgaaggag ggttggctgc acaaacgagg ggagtacatc aagacctggc
      181 ggccatgcta cttcctcctc aagaatgatg gcaccttcat tggctacaag gagcggccgc
      241 aggatgtgga ccaacgtgag gctcccctca acaacttctc tgtggcgcag tgccagctga
      301 tgaagacgga gcggccccgg cccaacacct tcatcatccg ctgcctgcag tggaccactg
      361 tcatcgaacg caccttccat gtggagactc ctgaggagcg ggaggagtgg acaaccgcca
      421 tccagactgt ggctgacggc ctcaagaagc aggaggagga ggagatggac ttccggtcgg
      481 gctcacccag tgacaactca ggggctgaag agatggaggt gtccctggcc aagcccaagc
      541 accgcgtgac catgaacgga tccatcgcca ccatggtgag caagggcgag gagctgttca
      601 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aanttcagcg
      661 tgtccggcga gtgcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      721 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      781 agtgctncag ccgctacc
//
