LOCUS       sequence-247291-       15798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15798)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX121
FEATURES             Location/Qualifiers
     source          1..15798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5912..5936)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5965..6054
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6088..6340
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6769..7021)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7489..8514)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7489..8514)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8524..8868)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9010..9027)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9010..9026)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9019..9041)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9166..9185)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9376..9397
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9644..9668
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11264..12412)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12711..13505)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13697..14464
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15157..15788
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg gatcatcacc atgttaaagt
     4621 cggtttgagc cgtggaggta attataataa aaataaaaac catcaacaac agcatgaaga
     4681 agacatggac ctccgacgag gtccatggac tgctgaagaa gacttcaccc tcatcaacta
     4741 catagctcac catggtgaag gtcgttggaa ttctctcgcc cactgtgctg gtatattttt
     4801 atgtgttttg tttcacctat tctttttata attaaccatc gacgacgttg atttaaaatt
     4861 gatgagacgc acgtttatcg tgttggttag aacttttatt ctgaaaagaa tgggtatata
     4921 tgttggactg tttgtggtag ataaggattt aagtgggcgt ggtttataat ttctttccgt
     4981 acttggtaat cttttaaaag cagagaattc atccaataaa caaataaagg caaaggaaag
     5041 gagaaataat tgacaagatt tattattaaa aatagagaag aaaaaacgtt caattgctac
     5101 ctgtatgaac tttaccaata attgagctca gaaatgagaa acctaatact tgaatgacat
     5161 ggaactgtaa taggtttaaa gcgaactggg aaaagttgca gattgagatg gctaaattat
     5221 ctccgcccag atgttcgacg tggaaacatc actcttgaag aacaacttgt gattcttgag
     5281 ctgcattctc gttggggcaa tcggtacgtt aaacattacc gagtgtagtt tcaggcttgt
     5341 ttgggtactc aattctgata atttatgtta tatatgtgta ggtggtcaaa aattgctgag
     5401 catttgccag gaagaactga caacgagatc aagaattact ggagaacccg agtgcaaaag
     5461 catgccaaac aactgaaatg cgacgtcaac agcaagcaat ttaaggatac catgcgttat
     5521 ttatggatgc caaggctagt tgagagaatt caagcagctg ccaccacaaa ctccaatcaa
     5581 gtgacaatta atccaaacaa taacaacgct attaataagc caagaatgag tgaaaattat
     5641 tcaattagca tggcagcttc atcagaaaac tcatttggga cgcctgtttc tgaccccggt
     5701 gattgttact acaattacgc cccagtcata agtagcaatc agcaagatta ttttcatcaa
     5761 agtactcaac tcggagaatc tttaaccatc cccacgggtc tagaattcca tactgcaatg
     5821 gatcagcaaa acacgagtca atggttggac ggtgggaatt tctctgacaa tttgtggaac
     5881 aatgaggaga cgtggttcat acagcccggg gacccagctt tcttgtacaa agtggttgat
     5941 aacagcgggt taattaacat cttttaccca tacgatgttc ctgactatgc gggctatccc
     6001 tatgacgtcc cggactatgc aggatcctat ccatatgacg ttccagatta cgctgctcag
     6061 tgcagcgctt agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
     6121 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     6181 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     6241 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     6301 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattagc ttcatcaacg
     6361 caagacatgc gcacgaccgt ctgacaggag aggaatttcc gacgagcaca gaaaggactt
     6421 gctcttggac gtaggcctat ttctcaggca catgtatcaa gtgttcggac gtgggttttc
     6481 gatggtgtat cagccgccgc caactgggag atgaggaggc tttcttgggg ggcagtcagc
     6541 agttcatttc acaagacaga ggaacttgta aggagatgca ctgatttatc ttggcgcaaa
     6601 ccagcaggac gaattagtgg gaatagcccg cgaatatcta agttatgcct gtcggcatga
     6661 gcagaaactt ccaattcgaa acagtttgga gaggttgttt ttgggcatac cttttgttag
     6721 tcagcctctc gattgctcat cgtcattaca cagtaccgaa gtttgatcga tctagtaaca
     6781 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
     6841 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
     6901 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca
     6961 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
     7021 ctgcttcgac gcactccttc tttactccac catctcgtcc ttattgaaaa cgtgggtagc
     7081 accaaaacga atcaagtcgc tggaactgaa gttaccaatc acgctggatg atttgccagt
     7141 tggattaatc ttgcctttcc ccgcatgaat aatattgatg aatgcatgcg tgaggggtat
     7201 ttcgattttg gcaatagctg caattgccgc gacatcctcc aacgagcata attcttcaga
     7261 aaaatagcga tgttccatgt tgtcagggca tgcatgatgc acgttatgag gtgacggtgc
     7321 taggcagtat tccctcaaag tttcatagtc agtatcatat tcatcattgc attcctgcaa
     7381 gagagaattg agacgcaatc cacacgctgc ggcaaccttc cggcgttcgt ggtctatttg
     7441 ctcttggacg ttgcaaacgt aagtgttgga tcccggtcgg catctactct attcctttgc
     7501 cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
     7561 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
     7621 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
     7681 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
     7741 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
     7801 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
     7861 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
     7921 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
     7981 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
     8041 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
     8101 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
     8161 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
     8221 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc
     8281 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
     8341 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
     8401 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa
     8461 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatcggg
     8521 gtcgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag
     8581 tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa
     8641 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
     8701 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
     8761 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
     8821 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
     8881 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
     8941 ttgacggatc tctaggacgc gtcgacgcgt cgacgcgtcg acgcgtcgac gcgtcctaga
     9001 agctaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     9061 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     9121 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gcccgctcct ttcgctttct
     9181 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     9241 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     9301 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     9361 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     9421 gctattcttt tgatttataa gggattttgc cgatttcgga accaccatca aacaggattt
     9481 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     9541 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc cagtacatta
     9601 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
     9661 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
     9721 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
     9781 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
     9841 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
     9901 tgatcaaata tcatctccct cgcagagatc cgaattatca gccttcttat tcatttctcg
     9961 cttaaccgtg acaggctgtc gatcttgaga actatgccga cataatagga aatcgctgga
    10021 taaagccgct gaggaagctg agtggcgcta tttctttaga agtgaacgtt gacgatatca
    10081 actcccctat ccattgctca ccgaatggta caggtcgggg acccgaagtt ccgactgtcg
    10141 gcctgatgca tccccggctg atcgacccca gatctggggc tgagaaagcc cagtaaggaa
    10201 acaactgtag gttcgagtcg cgagatcccc cggaaccaaa ggaagtaggt taaacccgct
    10261 ccgatcaggc cgagccacgc caggccgaga acattggttc ctgtaggcat cgggattggc
    10321 ggatcaaaca ctaaagctac tggaacgagc agaagtcctc cggccgccag ttgccaggcg
    10381 gtaaaggtga gcagaggcac gggaggttgc cacttgcggg tcagcacggt tccgaacgcc
    10441 atggaaaccg cccccgccag gcccgctgcg acgccgacag gatctagcgc tgcgtttggt
    10501 gtcaacacca acagcgccac gcccgcagtt ccgcaaatag cccccaggac cgccatcaat
    10561 cgtatcgggc tacctagcag agcggcagag atgaacacga ccatcagcgg ctgcacagcg
    10621 cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg aaataaacaa caagctccag
    10681 aatagcgaaa tattaagtgc gccgaggatg aagatgcgca tccaccagat tcccgttgga
    10741 atctgtcgga cgatcatcac gagcaataaa cccgccggca acgcccgcag cagcataccg
    10801 gcgacccctc ggcctcgctg ttcgggctcc acgaaaacgc cggacagatg cgccttgtga
    10861 gcgtccttgg ggccgtcctc ctgtttgaag accgacagcc caatgatctc gccgtcgatg
    10921 taggcgccga atgccacggc atctcgcaac cgttcagcga acgcctccat gggctttttc
    10981 tcctcgtgct cgtaaacgga cccgaacatc tctggagctt tcttcagggc cgacaatcgg
    11041 atctcgcgga aatcctgcac gtcggccgct ccaagccgtc gaatctgagc cttaatcaca
    11101 attgtcaatt ttaatcctct gtttatcggc agttcgtaga gcgcgccgtg cgtcccgagc
    11161 gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg ttcctgaaat gccagtaaag
    11221 cgctggctgc tgaaccccca gccggaactg accccacaag gccctagcgt ttgcaatgca
    11281 ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg
    11341 cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg cacatgaggc
    11401 ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag tcgaacatcc
    11461 gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg tagtggtcgc
    11521 cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac gttttcttgc
    11581 cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt
    11641 cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt
    11701 aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga
    11761 tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac accagttcgt
    11821 catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg ttgacgtgga
    11881 aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg
    11941 cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga
    12001 acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct
    12061 tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca
    12121 tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac
    12181 gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc agttgcacgc
    12241 tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga gccgacggac tggaaggttt
    12301 cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc
    12361 ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc attcaccctc
    12421 cttgcgggat tgccccgact cacgccgggg caatgtgccc ttattcctga tttgacccgc
    12481 ctggtgcctt ggtgtccaga taatccacct tatcggcaat gaagtcggtc ccgtagaccg
    12541 tctggccgtc cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat accagcgacc
    12601 ccttgcccaa atacttgccg tgggcctcgg cctgagagcc aaaacacttg atgcggaaga
    12661 agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca catctaggta ctaaaacaat
    12721 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
    12781 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
    12841 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
    12901 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
    12961 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
    13021 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
    13081 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
    13141 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
    13201 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
    13261 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
    13321 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
    13381 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
    13441 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
    13501 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
    13561 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
    13621 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
    13681 tgaaaccaca attatgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
    13741 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
    13801 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta tctctgctct cactgccgta
    13861 aaacatggca actgcagttc acttacaccg cttctcaacc cggtacgcac cagaaaatca
    13921 ttgatatggc catgaatggc gttggatgcc gggcaacagc ccgcattatg ggcgttggcc
    13981 tcaacacgat tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca
    14041 gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa cagtggggct atgtcggggc
    14101 taaatcgcgc cagcgctggc tgttttacgc gtatgacagt ctccggaaga cggttgttgc
    14161 gcacgtattc ggtgaacgca ctatggcgac gctggggcgt cttatgagcc tgctgtcacc
    14221 ctttgacgtg gtgatatgga tgacggatgg ctggccgctg tatgaatccc gcctgaaggg
    14281 aaagctgcac gtaatcagca agcgatatac gcagcgaatt gagcggcata acctgaatct
    14341 gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct
    14401 gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat
    14461 tacccaatta tgatagaatt tacaagctat aaggttattg tcctgggttt caagcattag
    14521 tccatgcaag tttttatgct ttgcccattc tatagatata ttgataagcg cgctgcctat
    14581 gccttgcccc ctgaaatcct tacatacggc gatatcttct atataaaaga tatattatct
    14641 tatcagtatt gtcaatatat tcaaggcaat ctgcctcctc atcctcttca tcctcttcgt
    14701 cttggtagct ttttaaatat ggcgcttcat agagtaattc tgtaaaggtc caattctcgt
    14761 tttcatacct cggtataatc ttacctatca cctcaaatgg ttcgctgggt ttatcgcacc
    14821 cccgaacacg agcacggcac ccgcgaccac tatgccaaga atgcccaagg taaaaattgc
    14881 cggccccgcc atgaagtccg tgaatgcccc gacggccgaa gtgaagggca ggccgccacc
    14941 caggccgccg ccctcactgc ccggcacctg gtcgctgaat gtcgatgcca gcacctgcgg
    15001 cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc catcccgtta ctgccccgat
    15061 cccggcaatg gcaaggactg ccagcgctgc catttttggg gtgaggccgt tcgcggccga
    15121 ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg gttcgagaag
    15181 ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa
    15241 caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa
    15301 acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc tcaaatgtca
    15361 ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat cgcgcccctc
    15421 atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc cccaggcttg
    15481 tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg
    15541 gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg
    15601 gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg gccaagtttt
    15661 ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc gacggcgttt
    15721 ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca accagcccgg
    15781 tgagcgtcgg aaaggcgc
//
