LOCUS       sequence-247302-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Golden Gate Cassette: SaCas9 expression cassette for 
            dicotyledonous plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR1CB0015
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(258..278)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(375..720)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(412..432)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tcggtcagca ggttgtagtc gaacagcagc ttcttcactc tctggattct atgccgcctc
       61 cgccgcttca gccttctggc gcctctcttg ctccgcctgc cctcgttgtt ttccacgttg
      121 gcctctttga acagccgcac gccggcatcg atcacgtccc gtgtctcgta gtcgatgatg
      181 ccgtagccca cgctggtgat gccgatgtcc aggcccagga tgtagttccg cttggctgct
      241 gggactccgt ggataccgac cttccgcttc ttctttgggg ccattgtatc gataattgta
      301 aatgtaattg taatgttgtt tgttgtttgt tgttgttggt aattgttgta aaaatgagct
      361 cttatactcg agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct
      421 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatgtc acatcaatcc
      481 acttgctttg tagacgtggt tggaacctct tctttttcca cgatgctcct cgtgggtggg
      541 ggtccatctt tgggaccact gtcggcagag agatcttgaa tgatagcctt tcctttatcg
      601 caatgatggc atttgtagga gccaccttcc ttttctactg tcctttcgat gaagtgacag
      661 atagctgggc aatggaatcc gaggaggttt cccgaaatta tcctttgttg aaaagtctca
      721 atagcccttt gatcttctga gactgtatct ttgacatttt tggagtagac cagagtgtcg
      781 tgctccacca tgttatcaca tcnatccact tgctttgtag acgtggttgg aacctcttct
      841 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagagaga
      901 tcttgaatga tagcctttcc tttatcgcaa tgatgggcat ttgtaggagc caccttccct
      961 tttctactgt cctttcgatg aagtgaca
//
